LOCUS       Exported                8460 bp ds-DNA     circular SYN 04-OCT-2024
DEFINITION  T4.
ACCESSION   T4
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 12, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..8460
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(747..1607)
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(1608..1712)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             1755..1847
                     /label=tnpA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=96.8"
                     /note="/match_length=6.5"
                     /note="/other=CDS"
     polyA_signal    complement(1868..1916)
                     /label=poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(1980..2772)
                     /label=NeoR/KanR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.7"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     CDS             complement(2823..3755)
                     /label=hRluc
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     promoter        complement(3806..4163)
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    4228..4279
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.9"
                     /note="/other=misc_feature"
     rep_origin      complement(4280..4312)
                     /direction=LEFT
                     /label=M13 ori (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.0"
                     /note="/match_length=7.3"
                     /note="/other=rep_origin"
     CDS             4317..4385
                     /label=large T antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.1"
                     /note="/match_length=3.2"
                     /note="/other=CDS"
     polyA_signal    4408..4529
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     terminator      complement(4551..4570)
                     /label=T7 terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=41.7"
                     /note="/other=terminator"
     CDS             complement(4578..4627)
                     /label=SET1 (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=39.7"
                     /note="/other=CDS"
     misc_feature    complement(4634..4658)
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=43.9"
                     /note="/other=misc_feature"
     CDS             complement(5807..7459)
                     /label=luciferase
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(7506..8005)
                     /label=PGK promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    complement(8062..8345)
                     /label=cer region
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
ORIGIN
        1 ttgaaatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccggttg
      721 ggcgtcgctt ggtcggtcat ttcgaattac caatgcttaa tcagtgaggc acctatctca
      781 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
      841 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
      901 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
      961 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1021 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1081 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1141 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat agttgtcaga
     1201 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     1261 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     1321 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     1381 ccacatagca gaactttgaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     1441 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     1501 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     1561 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     1621 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     1681 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     1741 gtctaagaaa ccattacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
     1801 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagaggat ccttatcgct
     1861 atcgattcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg
     1921 ccatcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
     1981 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     2041 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     2101 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
     2161 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     2221 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
     2281 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     2341 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     2401 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     2461 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     2521 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     2581 gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     2641 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     2701 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     2761 tcttgttcaa tccctccgga gcctccgcca cctgatccgc cacctccgct cccaccaccg
     2821 gtctgctcgt tcttcagcac gcgctccacg aagctcttga tgtacttacc catttcatct
     2881 ggagcgtcct cctggctgaa gtggaggccc ttcaccttca cgaactcggt gttagggaac
     2941 ttcttagctc cctcgacaat agcgttggaa aagaacccag ggtcggactc gatgaacatc
     3001 ttaggcagat cgtcgctggc ccgaaggtag gcgttgtagt tgcggacaat ctggacgacg
     3061 tcgggcttgc ctcccttaac gagagggatc tcgcgaggcc aggagagggt aggccgtcta
     3121 acctcgccct tctccttgaa tggctccagg taggcagcga actcctcagg ctccagtttc
     3181 cgcatgatct tgcttgggag catggtctcg acgaagaagt tattctcaag caccattttc
     3241 tcgccctctt cgctcttgat cagggcgata tcctcctcga tgtcaggcca ctcgtcccag
     3301 gactcgatca cgtccacgac actctcagca tggacgatgg ccttgatctt gtcttggtgc
     3361 tcgtaggagt agtgaaaggc cagacaagcc ccccagtcgt ggcccacaaa gatgattttc
     3421 tttggaaggt tcagcagctc gaaccaagcg gtgaggtact tgtagtgatc caggaggcga
     3481 tatgagccat tcccgctctt gccggactta cccattccga tcagatcagg gatgatgcat
     3541 ctagccacgg gctcgatgtg aggcacgacg tgcctccaca ggtagctgga ggcagcgtta
     3601 ccatgcagaa aaatcacggc gttctcggcg tgcttctcgg aatcatagta gttgatgaag
     3661 gagtccagca cgttcatttg cttgcagcga gcccaccact gaggcccagt gatcatgcgt
     3721 ttgcgttgct cggggtcgta caccttggaa gccatggtgg ctccagcctt tggttcgaga
     3781 ctgttgtgtc agaagaatca agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     3841 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     3901 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     3961 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
     4021 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
     4081 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
     4141 acagctggtt ctttccgcct cagaaggtac ctaaccaagt tcctctttca gaggttattt
     4201 caggccatgg tgctgcgcag atccgcgtat gcggtgtgaa ataccgcaca gatgcgtaag
     4261 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgattttacc
     4321 acatttgtag aggttttact tgcttaaaaa aacctcccac atctccccct gaacctgaaa
     4381 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4441 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4501 ggtttgtcca aactcatcaa tgtatcttat catgtctgct cgaagcggcc gccccaaggg
     4561 gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt cgggctttgt
     4621 tagcagccgg atcagcttgc atgcctgcag gtcgactcag aaacacacag ctttatatta
     4681 ttacaaaaaa gaaaaacacc tcaccttgtc tttgtttcgc agcacagaca gaaatcattt
     4741 taagacagag gatcacatag gcagttgtac acccagctga caaacacttt gggtctggat
     4801 ttcctccccc aaccacatta catcagcttt gacacatctg taaaacatct tctctgcaga
     4861 aagtcagacc ctaaagatca aacccaggga ggcaccctct ccccaggcca gctactctaa
     4921 ctcataaccc tctgaagaca cttggtaaag gcaacacagc tgacgagagc agtgctgcct
     4981 cagcagtagt gggctctggc cccgccaagg cagggaggac tagggccgag cttctgagaa
     5041 gccatccaga taggcaaggg agaaggggag ggggtgcagt ggggataaaa gaacctgatg
     5101 aaatggaaga aagcattacc ttgggagtta ccattttgaa ttattatata atcagactct
     5161 agcctggcat cacggagact ccaagagcca cggacaatgg gaaagaagtg tacttcttga
     5221 atgcaattca aagttatctg aacttttttc actgtttcta gattccactt tgcttgggaa
     5281 actacaagaa cttctttaat cagccatggc cttccaaaaa gtagagtgat gtggcaggca
     5341 agacagggac aagcaggccc acctggtaaa ggatggctct gacattagaa cctgttggct
     5401 gccctctcca ctgccaggga aggaagctgg ccatgcaggt gaagaaaggc accagccctg
     5461 tttcctgcct gaagggcagc atcaaggtca tagtgtccaa aggcccggaa gaggtcagtc
     5521 tcagatgggt gctggcagag tcctagccaa gtggccaggc tgaatccttc ctgagccacc
     5581 tgcaggaaga gtcaggaggg cgaggctctg gccactgcct tgcagctcac agctggttca
     5641 cacatgcatg caaagggcag gtaactaagt aaccaacccc cagaaagcag tttcatcgtt
     5701 tttgttttta tttttcccct ttcaacctgg ttttcggctc tggaagctgt tcctgtgcac
     5761 agttctattt cactcgaggc tagcgagctc gtttaaacaa ctagaattac acggcgatct
     5821 tgccgccctt cttggcctta atgagaatct cgcggatctt gcgggcgtcc aacttgccgg
     5881 tcagtccttt aggcacctcg tccacgaaca caacaccacc gcgcagcttc ttggcggttg
     5941 taacctggct ggccacatag tccacgatct ccttctcggt catggtttta ccgtgttcca
     6001 gcacgacgac tgcggcgggc agctcgccgg catcgtcgtc gggcaggccg gcgaccccgg
     6061 cgtcgaagat gttggggtgt tgcagcagga tgctctccag ttcggctggg gctacctggt
     6121 agcccttgta tttgatcagg ctcttcagcc ggtccacgat gaagaagtgc tcgtcctcgt
     6181 cccagtaggc gatgtcgccg ctgtgcagcc agccgtcctt gtcgatgaga gcgtttgtag
     6241 cctcggggtt gttaacgtag ccgctcatga tcatggggcc acggacgcac agctcgccgc
     6301 gctggttcac acccagtgtc ttaccggtgt ccaagtccac caccttagcc tcgaagaagg
     6361 gcaccacctt gcctactgcg ccaggcttgt cgtccccttc gggggtgatc agaatggcgc
     6421 tggttgtttc tgtcaggccg tagccctggc ggatgcctgg taggtggaag cgtttggcca
     6481 cggcctcacc tacctccttg ctgagcggcg ccccgccgct ggcgatctcg tgcaagttgc
     6541 ttaggtcgta cttgtcgatg agagtgctct tagcgaagaa gctaaatagt gtgggcacca
     6601 gcagggcaga ttgaatctta tagtcttgca agctgcgcaa gaatagctcc tcctcgaagc
     6661 ggtacatgag cacgacccga aagccgcaga tcaagtagcc cagcgtggtg aacatgccga
     6721 agccgtggtg aaatggcacc acgctgagga tagcggtgtc ggggatgatc tggttgccga
     6781 agatggggtc gcgggcatga ctgaatcgga cacaagcggt gcggtgcggt agggctacgc
     6841 ccttgggcaa tccggtactg ccactactgt tcatgatcag ggcgatggtt ttgtcccggt
     6901 cgaagctctc gggcacgaag tcgtactcgt tgaagccggg tggcaaatgg gaagtcacga
     6961 aggtgtacat gctttggaag ccctggtagt cggtcttgct atccatgatg atgatctttt
     7021 gtatgatcgg tagcttcttt tgcacgttga ggatcttttg cagccctttc ttgctcacga
     7081 atacgacggt gggctggctg atgcccatgc tgttcagcag ctcgcgctcg ttgtagatgt
     7141 cgttagctgg ggccacagcc acaccgatga acagggcacc caacacgggc atgaagaact
     7201 gcaagctatt ctcgctgcac accacgatcc gatggtttgt attcagccca tagcgcttca
     7261 tagcttctgc cagccgaacg ctcatctcga agtactcggc gtaggtaatg tccacctcga
     7321 tatgtgcgtc ggtaaaggcg atggtgccgg gcaccagggc gtagcgcttc atggctttgt
     7381 gcagctgctc gccggcggtc ccgtcttcga gtgggtagaa tggcgctggg cccttcttaa
     7441 tgtttttggc atcttccatg gtggctttac caacagtacc ggattgccaa gcttgggctg
     7501 caggtcgaaa ggcccggaga tgaggaagag gagaacagcg cggcagacgt gcgcttttga
     7561 agcgtgcaga atgccgggcc tccggaggac cttcgggcgc ccgccccgcc cctgagcccg
     7621 cccctgagcc cgcccccgga cccacccctt cccagcctct gagcccagaa agcgaaggag
     7681 caaagctgct attggccgct gccccaaagg cctacccgct tccattgctc agcggtgctg
     7741 tccatctgca cgagactagt gagacgtgct acttccattt gtcacgtcct gcacgacgcg
     7801 agctgcgggg cgggggggaa cttcctgact aggggaggag tagaaggtgg cgcgaagggg
     7861 ccaccaaaga acggagccgg ttggcgccta ccggtggatg tggaatgtgt gcgaggccag
     7921 aggccacttg tgtagcgcca agtgcccagc ggggctgcta aagcgcatgc tccagactgc
     7981 cttgggaaaa gcgcctcccc tacccggtag atctcgatcc tctacgccgg acgcatcgtg
     8041 gccggagtac tgcggtttat ctcgatggct acgagggcag acagtaagtg gatttaccat
     8101 aatcccttaa ttgtacgcac cgctaaaacg cgttcagcgc gatcacggca gcagacaggt
     8161 aaaaatggca acaaaccacc cgaaaaactg ccgcaatcgc gcctgataaa ttttaaccgt
     8221 atgaatacct atgcaaccag agggtacagg ccacattacc cccacttaat ccactgaagc
     8281 tgccattttt catggtttca ccatcccagc gaagggccat ccagcgtgcg ttcctgtatt
     8341 tccggactat aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     8401 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
//
