LOCUS       Exported                8206 bp ds-DNA     circular SYN 04-OCT-2024
DEFINITION  T5.
ACCESSION   T5
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 12, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..8206
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(747..1607)
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(1608..1712)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             1755..1847
                     /label=tnpA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=96.8"
                     /note="/match_length=6.5"
                     /note="/other=CDS"
     polyA_signal    complement(1868..1916)
                     /label=poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(1980..2772)
                     /label=NeoR/KanR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.7"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     CDS             complement(2823..3755)
                     /label=hRluc
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     promoter        complement(3806..4163)
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    4228..4279
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.9"
                     /note="/other=misc_feature"
     rep_origin      complement(4280..4312)
                     /direction=LEFT
                     /label=M13 ori (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.0"
                     /note="/match_length=7.3"
                     /note="/other=rep_origin"
     CDS             4317..4385
                     /label=large T antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.1"
                     /note="/match_length=3.2"
                     /note="/other=CDS"
     polyA_signal    4408..4529
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     terminator      complement(4551..4570)
                     /label=T7 terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=41.7"
                     /note="/other=terminator"
     CDS             complement(4578..4627)
                     /label=SET1 (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=39.7"
                     /note="/other=CDS"
     misc_feature    complement(4634..4657)
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=42.1"
                     /note="/other=misc_feature"
     CDS             complement(5553..7205)
                     /label=luciferase
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(7252..7751)
                     /label=PGK promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    complement(7808..8091)
                     /label=cer region
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
ORIGIN
        1 ttgaaatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccggttg
      721 ggcgtcgctt ggtcggtcat ttcgaattac caatgcttaa tcagtgaggc acctatctca
      781 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
      841 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
      901 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
      961 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1021 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1081 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1141 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat agttgtcaga
     1201 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     1261 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     1321 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     1381 ccacatagca gaactttgaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     1441 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     1501 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     1561 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     1621 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     1681 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     1741 gtctaagaaa ccattacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
     1801 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagaggat ccttatcgct
     1861 atcgattcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg
     1921 ccatcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
     1981 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     2041 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     2101 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
     2161 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     2221 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
     2281 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     2341 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     2401 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     2461 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     2521 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     2581 gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     2641 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     2701 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     2761 tcttgttcaa tccctccgga gcctccgcca cctgatccgc cacctccgct cccaccaccg
     2821 gtctgctcgt tcttcagcac gcgctccacg aagctcttga tgtacttacc catttcatct
     2881 ggagcgtcct cctggctgaa gtggaggccc ttcaccttca cgaactcggt gttagggaac
     2941 ttcttagctc cctcgacaat agcgttggaa aagaacccag ggtcggactc gatgaacatc
     3001 ttaggcagat cgtcgctggc ccgaaggtag gcgttgtagt tgcggacaat ctggacgacg
     3061 tcgggcttgc ctcccttaac gagagggatc tcgcgaggcc aggagagggt aggccgtcta
     3121 acctcgccct tctccttgaa tggctccagg taggcagcga actcctcagg ctccagtttc
     3181 cgcatgatct tgcttgggag catggtctcg acgaagaagt tattctcaag caccattttc
     3241 tcgccctctt cgctcttgat cagggcgata tcctcctcga tgtcaggcca ctcgtcccag
     3301 gactcgatca cgtccacgac actctcagca tggacgatgg ccttgatctt gtcttggtgc
     3361 tcgtaggagt agtgaaaggc cagacaagcc ccccagtcgt ggcccacaaa gatgattttc
     3421 tttggaaggt tcagcagctc gaaccaagcg gtgaggtact tgtagtgatc caggaggcga
     3481 tatgagccat tcccgctctt gccggactta cccattccga tcagatcagg gatgatgcat
     3541 ctagccacgg gctcgatgtg aggcacgacg tgcctccaca ggtagctgga ggcagcgtta
     3601 ccatgcagaa aaatcacggc gttctcggcg tgcttctcgg aatcatagta gttgatgaag
     3661 gagtccagca cgttcatttg cttgcagcga gcccaccact gaggcccagt gatcatgcgt
     3721 ttgcgttgct cggggtcgta caccttggaa gccatggtgg ctccagcctt tggttcgaga
     3781 ctgttgtgtc agaagaatca agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     3841 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     3901 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     3961 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
     4021 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
     4081 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
     4141 acagctggtt ctttccgcct cagaaggtac ctaaccaagt tcctctttca gaggttattt
     4201 caggccatgg tgctgcgcag atccgcgtat gcggtgtgaa ataccgcaca gatgcgtaag
     4261 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgattttacc
     4321 acatttgtag aggttttact tgcttaaaaa aacctcccac atctccccct gaacctgaaa
     4381 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4441 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4501 ggtttgtcca aactcatcaa tgtatcttat catgtctgct cgaagcggcc gccccaaggg
     4561 gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt cgggctttgt
     4621 tagcagccgg atcagcttgc atgcctgcag gtcgactttg tggaaaactc aacctttatt
     4681 attacctgcc tagtgcaggg gattaaaatt gcctcaagct aggtccatat attagtgtaa
     4741 aaatctgtgt ctctcccccc aaaaatgtac aaaccccaag tgattataga aaaatcaatg
     4801 tggcagctac actagagatg tccaacccca aggctatggg ccgttgctcc ctctttcccc
     4861 ccaatcccaa tatttactcc acagaaacgt ataggcttag agaagttcta gctacaagac
     4921 tgcaagtggg aagtgggggt gactgagggc tggggcgggg ctacccccag gtccagtgtt
     4981 tcaggtgatg gaggaggaag aagcccaagc tgaggacaag tacaggagca ggcacaagga
     5041 aacattttcc tctccttctt gtcctcctca cccccagatt ctcagccagt ggggtccagt
     5101 gtgtgacagg gagagagaag ctgggggcgc agccacctct ccaggaatca gtgttgtcca
     5161 gtggggccca ggtgctccaa tgggccatgt ccagtgtcta tcctatattt ctccccaccc
     5221 ttccccaagg gatggctaga ggagggtgga gaggatccca tccacaggac cagctgggtg
     5281 ggcgacaggg gcttctagga aggaagaaca ggaaggggac atgagagtgt gtccaggaag
     5341 gggagaaggg gcatcagctc cctgggtggg cccagcccac ctgcagtctc actgcctcta
     5401 ttagaggtga tgggctgtcg ggaggggaag ggcagaggtg ccattgtgtc ctcttgtttt
     5461 agtgtcactg gcttcccctg gatcgctccc tgagggaggg gcagctggat gtgtgatact
     5521 cgaggctagc gagctcgttt aaacaactag aattacacgg cgatcttgcc gcccttcttg
     5581 gccttaatga gaatctcgcg gatcttgcgg gcgtccaact tgccggtcag tcctttaggc
     5641 acctcgtcca cgaacacaac accaccgcgc agcttcttgg cggttgtaac ctggctggcc
     5701 acatagtcca cgatctcctt ctcggtcatg gttttaccgt gttccagcac gacgactgcg
     5761 gcgggcagct cgccggcatc gtcgtcgggc aggccggcga ccccggcgtc gaagatgttg
     5821 gggtgttgca gcaggatgct ctccagttcg gctggggcta cctggtagcc cttgtatttg
     5881 atcaggctct tcagccggtc cacgatgaag aagtgctcgt cctcgtccca gtaggcgatg
     5941 tcgccgctgt gcagccagcc gtccttgtcg atgagagcgt ttgtagcctc ggggttgtta
     6001 acgtagccgc tcatgatcat ggggccacgg acgcacagct cgccgcgctg gttcacaccc
     6061 agtgtcttac cggtgtccaa gtccaccacc ttagcctcga agaagggcac caccttgcct
     6121 actgcgccag gcttgtcgtc cccttcgggg gtgatcagaa tggcgctggt tgtttctgtc
     6181 aggccgtagc cctggcggat gcctggtagg tggaagcgtt tggccacggc ctcacctacc
     6241 tccttgctga gcggcgcccc gccgctggcg atctcgtgca agttgcttag gtcgtacttg
     6301 tcgatgagag tgctcttagc gaagaagcta aatagtgtgg gcaccagcag ggcagattga
     6361 atcttatagt cttgcaagct gcgcaagaat agctcctcct cgaagcggta catgagcacg
     6421 acccgaaagc cgcagatcaa gtagcccagc gtggtgaaca tgccgaagcc gtggtgaaat
     6481 ggcaccacgc tgaggatagc ggtgtcgggg atgatctggt tgccgaagat ggggtcgcgg
     6541 gcatgactga atcggacaca agcggtgcgg tgcggtaggg ctacgccctt gggcaatccg
     6601 gtactgccac tactgttcat gatcagggcg atggttttgt cccggtcgaa gctctcgggc
     6661 acgaagtcgt actcgttgaa gccgggtggc aaatgggaag tcacgaaggt gtacatgctt
     6721 tggaagccct ggtagtcggt cttgctatcc atgatgatga tcttttgtat gatcggtagc
     6781 ttcttttgca cgttgaggat cttttgcagc cctttcttgc tcacgaatac gacggtgggc
     6841 tggctgatgc ccatgctgtt cagcagctcg cgctcgttgt agatgtcgtt agctggggcc
     6901 acagccacac cgatgaacag ggcacccaac acgggcatga agaactgcaa gctattctcg
     6961 ctgcacacca cgatccgatg gtttgtattc agcccatagc gcttcatagc ttctgccagc
     7021 cgaacgctca tctcgaagta ctcggcgtag gtaatgtcca cctcgatatg tgcgtcggta
     7081 aaggcgatgg tgccgggcac cagggcgtag cgcttcatgg ctttgtgcag ctgctcgccg
     7141 gcggtcccgt cttcgagtgg gtagaatggc gctgggccct tcttaatgtt tttggcatct
     7201 tccatggtgg ctttaccaac agtaccggat tgccaagctt gggctgcagg tcgaaaggcc
     7261 cggagatgag gaagaggaga acagcgcggc agacgtgcgc ttttgaagcg tgcagaatgc
     7321 cgggcctccg gaggaccttc gggcgcccgc cccgcccctg agcccgcccc tgagcccgcc
     7381 cccggaccca ccccttccca gcctctgagc ccagaaagcg aaggagcaaa gctgctattg
     7441 gccgctgccc caaaggccta cccgcttcca ttgctcagcg gtgctgtcca tctgcacgag
     7501 actagtgaga cgtgctactt ccatttgtca cgtcctgcac gacgcgagct gcggggcggg
     7561 ggggaacttc ctgactaggg gaggagtaga aggtggcgcg aaggggccac caaagaacgg
     7621 agccggttgg cgcctaccgg tggatgtgga atgtgtgcga ggccagaggc cacttgtgta
     7681 gcgccaagtg cccagcgggg ctgctaaagc gcatgctcca gactgccttg ggaaaagcgc
     7741 ctcccctacc cggtagatct cgatcctcta cgccggacgc atcgtggccg gagtactgcg
     7801 gtttatctcg atggctacga gggcagacag taagtggatt taccataatc ccttaattgt
     7861 acgcaccgct aaaacgcgtt cagcgcgatc acggcagcag acaggtaaaa atggcaacaa
     7921 accacccgaa aaactgccgc aatcgcgcct gataaatttt aaccgtatga atacctatgc
     7981 aaccagaggg tacaggccac attaccccca cttaatccac tgaagctgcc atttttcatg
     8041 gtttcaccat cccagcgaag ggccatccag cgtgcgttcc tgtatttccg gactataaaa
     8101 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     8161 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttc
//
