LOCUS       Exported               13141 bp ds-DNA     circular SYN 19-NOV-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13141)
  AUTHORS   MINYH
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Mar 13, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13141
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     492..511
                     /label=PCDNA3.0-F2
     primer_bind     494..514
                     /label=PCDNA3.1-F
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     complement(2076..2095)
                     /label=PLKO1-R
     primer_bind     2088..2111
                     /label=U6-pll3-7
     promoter        2224..3405
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     primer_bind     complement(2296..2318)
                     /label=pCDH1-R
     primer_bind     complement(2341..2360)
                     /label=EF1A-R
     intron          2454..3396
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3353..3373
                     /label=EF-1aFwd(PEF-F)
     CDS             3418..3465
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             3472..7416
                     /codon_start=1
                     /label=AS CPF1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYRNRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIRLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILREFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLRTISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIARGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLANLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEAYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRNGSPKKKRKV"
     primer_bind     complement(3976..4001)
                     /label=dAsCas12a(E174R)-R
     primer_bind     3976..4001
                     /label=dAsCas12a(E174R)-F
     misc_feature    3988..3990
                     /label=E174R
     primer_bind     complement(4223..4247)
                     /label=As-hD256R-R
     primer_bind     4223..4247
                     /label=As-hD256R-F
     misc_feature    4234..4236
                     /label=D256R
     primer_bind     complement(4414..4445)
                     /label=As-hE321R-R
     primer_bind     4414..4445
                     /label=As-hE321R-F
     misc_feature    4429..4431
                     /label=E321R
     primer_bind     complement(4571..4594)
                     /label=As-hE371R-R
     primer_bind     4571..4594
                     /label=As-hE371R-F
     misc_feature    4582..4584
                     /label=E371R
     CDS             7444..8160
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             8188..8235
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             8254..8319
                     /codon_start=1
                     /product="three tandem FLAG epitope tags, followed by an 
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    8326..8893
                     /label=IRES
     primer_bind     complement(8341..8364)
                     /label=PQCXIP-R (=pQC-R)
     primer_bind     complement(8366..8385)
                     /label=pIRES2-EGFP-P3
     primer_bind     complement(8415..8434)
                     /label=IRESrev
     primer_bind     8768..8788
                     /label=IRES-F
     CDS             8913..9512
                     /codon_start=1
                     /label=PuroR
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    9531..10119
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9566..9588)
                     /label=PCDNA3-4-R
     primer_bind     complement(9584..9604)
                     /label=WPRE-R
     primer_bind     complement(9607..9624)
                     /label=GIPZ sequencing primer
     CDS             complement(10002..10013)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10194..10427
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     10285..10304
                     /label=PCDNA3.0-F2
     primer_bind     10287..10307
                     /label=PCDNA3.1-F
     primer_bind     complement(10546..10564)
                     /label=pBRforEco
     primer_bind     complement(10598..10617)
                     /label=PBR322F
     primer_bind     complement(10614..10633)
                     /label=PTRG-R
     promoter        10632..10736
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10692..10710)
                     /label=BLA-Prorev
     CDS             10737..11597
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11043..11065
                     /label=AmpR-1F
     primer_bind     complement(11268..11286)
                     /label=AmpR-1R
     primer_bind     11490..11513
                     /label=Primer Bla M1
     primer_bind     11490..11513
                     /label=PrimerBlaM1
     primer_bind     complement(11695..11718)
                     /label=Primer Bla RV
     primer_bind     complement(11695..11718)
                     /label=PrimerBlaRV
     rep_origin      11768..12356
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12543..12555
                     /label=PCAMBIA2300-5
     primer_bind     12619..12638
                     /label=SV40pro-F
     primer_bind     complement(12642..12653)
                     /label=SV40pro-F
     primer_bind     12674..12691
                     /label=SV40ori-3for
     protein_bind    12767..12788
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     12797..12816
                     /label=PCAMBIA1301-F(即为P1)
     primer_bind     12798..12819
                     /label=SRprimer
     primer_bind     12800..12819
                     /label=ptarget-R
     promoter        12803..12833
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     12817..12836
                     /label=Y-M13R-48
     primer_bind     12824..12841
                     /label=PCAMBIA1300-5
     protein_bind    12841..12857
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12844..12863
                     /label=pQE30-F
     primer_bind     12846..12869
                     /label=M13R-48
     primer_bind     12846..12867
                     /label=PQ60-F
     primer_bind     12865..12881
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12865..12881
                     /label=M13Rev(-26)
     primer_bind     12865..12881
                     /label=pDONR222-R
     primer_bind     12872..12890
                     /label=M13mp18-R
     primer_bind     12916..12935
                     /label=PIRES-R
     primer_bind     12921..12940
                     /label=PCI-NEO-R
     primer_bind     12931..12950
                     /label=EBVrev
     primer_bind     12939..12958
                     /label=pCMV-R
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     2581 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     2641 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     2701 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     2761 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     2821 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     2881 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     2941 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     3001 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     3061 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     3121 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     3181 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     3241 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     3301 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     3361 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaagcgg ccgcatgaag
     3421 cgacctgccg ccacaaagaa ggctggacag gctaagaaga agaaagctag cactcagttc
     3481 gagggcttca caaacctgta tcaagtgtcc aagaccctcc gcttcgagct gatccctcag
     3541 ggtaagaccc tgaagcatat acaggagcag ggtttcatcg aggaggacaa ggccaggaat
     3601 gatcactaca aggagctgaa gcccatcatt gatcgcatct acaagacata cgcagatcaa
     3661 tgtctgcaac tcgtccagct ggattgggag aatctgtcag ccgcaatcga cagttatcgg
     3721 aaagagaaga cagaagaaac caggaacgcc ctcatcgagg aacaagcaac ctaccggaat
     3781 gcaatacacg actatttcat cggcaggact gacaatctga ctgacgccat caataagaga
     3841 cacgcagaaa tctataaggg cctctttaag gccgaactct tcaacggcaa agtcctcaaa
     3901 cagctgggca cagttaccac taccgagcac gagaatgccc tgctccggag cttcgacaag
     3961 ttcaccactt acttctctgg gttctacaga aatcggaaga acgtgttcag cgcagaggac
     4021 atctccacag ccattccaca ccggatcgtc caggataact ttcccaaatt caaagagaat
     4081 tgccatatct ttaccaggct gatcactgcc gttccatctc tgagggaaca ctttgagaac
     4141 gtgaagaaag ccatcgggat attcgtgtct acttccatcg aggaagtgtt ctctttccct
     4201 ttctacaatc agctgctgac ccagacccag atcagactct ataaccagct gctgggaggt
     4261 atcagcaggg aggcaggcac cgagaagatc aagggactga atgaggtcct gaacctcgcc
     4321 attcagaaga acgatgagac agcacacatc atcgcctctc tgccacatcg cttcattcct
     4381 ctgttcaaac agattctctc tgatcggaac accctcagct ttatcctgag agagtttaag
     4441 tccgatgaag aggttattca gtctttctgt aagtacaaga ctctgctgcg caatgagaac
     4501 gtgctggaga ctgccgaagc tctgtttaac gaactgaact ccatcgacct cacccacatc
     4561 ttcatcagcc acaagaagct gagaacaatt agcagtgccc tgtgtgacca ttgggatacc
     4621 ctgcgcaacg ccctgtacga gaggagaata tccgagctga caggtaagat aaccaagagc
     4681 gccaaagaga aagtccaaag gtccctgaaa catgaggaca taaacctgca ggagattatc
     4741 tcagcagctg gtaaggaact ctcagaggct ttcaagcaga agacctccga aattctctcc
     4801 catgcccacg cagcactcga tcaaccactg ccaaccaccc tgaagaaaca agaggagaag
     4861 gaaatcctga agagccagct ggacagcctc ctcgggctgt accatctgct cgattggttc
     4921 gccgtggacg aaagtaacga ggtggaccca gagttctcag caagactgac cggcatcaag
     4981 ctggagatgg agcccagtct ctccttctac aacaaagcta gaaactacgc caccaagaag
     5041 ccttattctg tggagaagtt taagctgaac ttccagatgc ccactctggc ttccggttgg
     5101 gacgtcaaca aggagaagaa taacggtgca atcctgttcg tgaagaacgg actgtactac
     5161 ctgggcatca tgccaaagca gaagggtcgc tacaaagccc tctcatttga acccacagag
     5221 aagacaagcg aaggatttga caagatgtat tacgattact ttcccgatgc cgctaagatg
     5281 atccctaagt gcagcaccca gctgaaggcc gtcaccgctc atttccagac ccatactaca
     5341 ccaattctgc tgtctaacaa cttcatagag ccactggaga taaccaagga aatctacgat
     5401 ctgaacaatc cagagaagga gcccaagaag tttcagaccg cctacgctaa gaagaccgga
     5461 gatcagaaag gttatcggga ggccctgtgc aaatggatcg acttcacacg cgatttcctc
     5521 agcaagtata caaagaccac tagcatagac ctgtccagcc tcaggccctc aagtcagtac
     5581 aaggatctcg gtgagtacta cgccgaactc aacccactgc tctaccacat tagctttcag
     5641 aggatcgccg agaaagagat catggatgca gtggagactg gcaagctgta tctgtttcag
     5701 atatacaaca aagacttcgc taaaggacac cacggtaagc caaacctgca tactctctat
     5761 tggaccggac tcttcagtcc agagaatctc gccaagacct caatcaagct gaacggtcag
     5821 gcagagctgt tctatagacc taagagtagg atgaaacgga tggcacatag actcggcgag
     5881 aagatgctga acaagaagct gaaagaccag aagactccca ttccagatac actgtaccag
     5941 gagctgtatg attacgtgaa tcaccggctc tcccacgacc tgtctgatga agccagggct
     6001 ctcctgccta acgtgataac taaagaggtg agccatgaga tcatcaaaga ccggagattc
     6061 acatccgaca agttcttctt tcatgttcca attacactga actatcaagc agcaaattct
     6121 cccagcaagt ttaaccaaag ggtgaacgcc tacctgaagg aacatcccga aacacccatc
     6181 attggaattg ctagaggtga gcgcaatctc atctacataa ccgtgatcga ctccacaggt
     6241 aagattctcg agcagaggtc cctcaacact attcagcagt ttgattatca gaagaaactc
     6301 gacaacagag agaaagagag agtcgctgcc agacaagcat ggagtgtcgt tggcacaatc
     6361 aaagacctga agcagggata cctgagccaa gttatacacg aaattgtgga tctgatgatc
     6421 cactaccagg ctgttgtggt gctggcgaac ctcaactttg gtttcaagtc taagaggaca
     6481 gggatcgctg agaaagctgt gtaccagcag tttgagaaga tgctcattga taaactgaac
     6541 tgtctcgtgc tcaaagacta ccctgccgag aaggtgggag gagtcctgaa tccttatcag
     6601 ctgactgacc agtttacctc cttcgctaag atgggcaccc aatcaggctt tctgttctac
     6661 gtgcctgcac cctacaccag taagattgat ccactgacag ggttcgttga tcctttcgtg
     6721 tggaagacca taaagaacca cgaaagcaga aagcacttcc tggaaggctt cgacttcctg
     6781 cattatgacg tcaagactgg agacttcata ctgcatttca agatgaatag aaacctgtca
     6841 tttcagagag gactgcctgg gttcatgcca gcttgggaca ttgtctttga gaagaatgag
     6901 acacagtttg acgccaaagg cactccattc attgctggca agcggatcgt ccctgtgata
     6961 gagaaccaca gattcactgg aagatacagg gacctgtacc cagcaaatga actcattgcc
     7021 ctcctggaag agaaagggat cgtgttccgg gatgggtcta acatcctgcc aaagctcctg
     7081 gagaatgacg attcacatgc cattgataca atggttgccc tgatccgcag tgtgctgcaa
     7141 atgaggaata gcaatgcagc caccggagag gcctacatca atagtccagt tcgggacctc
     7201 aatggcgtgt gtttcgatag tagattccag aaccctgagt ggcctatgga cgctgatgct
     7261 aacggtgctt atcatattgc cctgaagggc cagctcctgc tgaaccacct caaggagtct
     7321 aaggacctga agctccagaa tggcatttct aatcaggatt ggctggccta tatccaggaa
     7381 ctgaggaacg gcagtcccaa gaagaagagg aaggtgagtc tagaggcgct accggtcgcc
     7441 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     7501 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     7561 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     7621 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     7681 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     7741 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     7801 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     7861 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     7921 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     7981 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     8041 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     8101 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     8161 tcgaattcta agccaggatt ccatatgaag cgacctgccg ccacaaagaa ggctggacag
     8221 gctaagaaga agaaacaacg cgtgcagcta agcgactaca aagaccatga cggtgattat
     8281 aaagatcatg acatcgatta caaggatgac gatgacaagt aaggatccct cccccccccc
     8341 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
     8401 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
     8461 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
     8521 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
     8581 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
     8641 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
     8701 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
     8761 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta
     8821 gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa
     8881 aacacgatga taaggatcca ccggagctta ccatgaccga gtacaagccc acggtgcgcc
     8941 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
     9001 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
     9061 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     9121 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     9181 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     9241 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     9301 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     9361 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     9421 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     9481 cctggtgcat gacccgcaag cccggtgcct gacgcccatc gatagatcct aatcaacctc
     9541 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     9601 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     9661 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     9721 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     9781 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     9841 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     9901 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     9961 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
    10021 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
    10081 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tgagatcctt taagaccaat
    10141 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
    10201 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
    10261 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
    10321 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
    10381 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
    10441 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc
    10501 cgggttaatt aaggaaaggg ctagatcatt cttgaagacg aaagggcctc gtgatacgcc
    10561 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
    10621 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
    10681 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
    10741 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
    10801 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
    10861 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
    10921 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg
    10981 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
    11041 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
    11101 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
    11161 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
    11221 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
    11281 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
    11341 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
    11401 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
    11461 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
    11521 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
    11581 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
    11641 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
    11701 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
    11761 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
    11821 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
    11881 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
    11941 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
    12001 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
    12061 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
    12121 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
    12181 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
    12241 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
    12301 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
    12361 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
    12421 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
    12481 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
    12541 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agcaagctca
    12601 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
    12661 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tccccgtggc
    12721 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
    12781 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
    12841 ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg aatttcacaa
    12901 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
    12961 atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc
    13021 cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct
    13081 ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag
    13141 t
//
