LOCUS       Exported               12859 bp ds-DNA     circular SYN 01-DEC-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12859)
  AUTHORS   MINYH
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Mar 13, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12859
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     492..511
                     /label=PCDNA3.0-F2
     primer_bind     494..514
                     /label=PCDNA3.1-F
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     complement(2076..2095)
                     /label=PLKO1-R
     primer_bind     2088..2111
                     /label=U6-pll3-7
     promoter        2224..3405
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     primer_bind     complement(2296..2318)
                     /label=pCDH1-R
     primer_bind     complement(2341..2360)
                     /label=EF1A-R
     intron          2454..3396
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3353..3373
                     /label=EF-1aFwd(PEF-F)
     CDS             3418..3465
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             3472..7152
                     /codon_start=1
                     /label=dLbCas12a
                     /translation="SKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAED
                     YKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKEI
                     AKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFSE
                     EAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEGE
                     FFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVLS
                     DRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKNG
                     PAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQL
                     QEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKDL
                     LDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYSK
                     DKFKLYFQNPQFMRGWDKDVETDRRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNGN
                     YEKINYKLLPGPNKMLPRVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKLI
                     DFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLVE
                     EGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKKE
                     ELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKIN
                     TEVRVLLKHDDNPYVIGIARGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTDY
                     HSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALADLNSGF
                     KNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQN
                     GFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALDY
                     KNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQQ
                     GDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVAFLISPVKNSDGIFYDSRNY
                     EAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTSV
                     KH"
     misc_feature    5062..5064
                     /label=G532R
     misc_feature    5080..5082
                     /label=K538V
     misc_feature    5092..5094
                     /label=Y542R
     misc_feature    5251..5253
                     /label=K595R
     CDS             7162..7878
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             7906..7953
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7972..8037
                     /codon_start=1
                     /product="three tandem FLAG epitope tags, followed by an 
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    8044..8611
                     /label=IRES
     primer_bind     complement(8059..8082)
                     /label=PQCXIP-R (=pQC-R)
     primer_bind     complement(8084..8103)
                     /label=pIRES2-EGFP-P3
     primer_bind     complement(8133..8152)
                     /label=IRESrev
     primer_bind     8486..8506
                     /label=IRES-F
     CDS             8631..9230
                     /codon_start=1
                     /label=PuroR
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    9249..9837
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9284..9306)
                     /label=PCDNA3-4-R
     primer_bind     complement(9302..9322)
                     /label=WPRE-R
     primer_bind     complement(9325..9342)
                     /label=GIPZ sequencing primer
     CDS             complement(9720..9731)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9912..10145
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     10003..10022
                     /label=PCDNA3.0-F2
     primer_bind     10005..10025
                     /label=PCDNA3.1-F
     primer_bind     complement(10264..10282)
                     /label=pBRforEco
     primer_bind     complement(10316..10335)
                     /label=PBR322F
     primer_bind     complement(10332..10351)
                     /label=PTRG-R
     promoter        10350..10454
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10410..10428)
                     /label=BLA-Prorev
     CDS             10455..11315
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10761..10783
                     /label=AmpR-1F
     primer_bind     complement(10986..11004)
                     /label=AmpR-1R
     primer_bind     11208..11231
                     /label=PrimerBlaM1
     primer_bind     11208..11231
                     /label=Primer Bla M1
     primer_bind     complement(11413..11436)
                     /label=PrimerBlaRV
     primer_bind     complement(11413..11436)
                     /label=Primer Bla RV
     rep_origin      11486..12074
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12261..12273
                     /label=PCAMBIA2300-5
     primer_bind     12337..12356
                     /label=SV40pro-F
     primer_bind     complement(12360..12371)
                     /label=SV40pro-F
     primer_bind     12392..12409
                     /label=SV40ori-3for
     protein_bind    12485..12506
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     12515..12534
                     /label=PCAMBIA1301-F(即为P1)
     primer_bind     12516..12537
                     /label=SRprimer
     primer_bind     12518..12537
                     /label=ptarget-R
     promoter        12521..12551
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     12535..12554
                     /label=Y-M13R-48
     primer_bind     12542..12559
                     /label=PCAMBIA1300-5
     protein_bind    12559..12575
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12562..12581
                     /label=pQE30-F
     primer_bind     12564..12587
                     /label=M13R-48
     primer_bind     12564..12585
                     /label=PQ60-F
     primer_bind     12583..12599
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12583..12599
                     /label=pDONR222-R
     primer_bind     12583..12599
                     /label=M13Rev(-26)
     primer_bind     12590..12608
                     /label=M13mp18-R
     primer_bind     12634..12653
                     /label=PIRES-R
     primer_bind     12639..12658
                     /label=PCI-NEO-R
     primer_bind     12649..12668
                     /label=EBVrev
     primer_bind     12657..12676
                     /label=pCMV-R
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     2581 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     2641 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     2701 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     2761 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     2821 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     2881 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     2941 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     3001 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     3061 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     3121 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     3181 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     3241 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     3301 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     3361 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaagcgg ccgcatgaag
     3421 cgacctgccg ccacaaagaa ggctggacag gctaagaaga agaaagctag ctcaaagctg
     3481 gagaaattca ccaactgtta tagcctgtct aagaccctgc gcttcaaggc aatcccagtg
     3541 ggcaagacac aagagaacat tgacaacaaa cggctcctgg tggaggatga gaagagggct
     3601 gaagattaca agggcgttaa gaagctgctg gataggtact atctgtcatt catcaacgat
     3661 gtcctccaca gtatcaagct gaagaatctg aacaattaca tttctctgtt ccggaagaag
     3721 acacggaccg agaaggagaa caaagagctg gagaatctgg agatcaacct gaggaaagaa
     3781 atagctaagg ctttcaaagg gaacgagggt tacaagtccc tgttcaagaa agacattatc
     3841 gagactattc tgcctgagtt cctggacgat aaagatgaga tcgccctcgt caattccttc
     3901 aatgggttta ccacagcctt taccggcttc ttcgacaata gagagaatat gttctctgaa
     3961 gaggccaaat ccactagcat cgcctttcgc tgcataaacg agaacctgac taggtacatc
     4021 agcaatatgg acatctttga gaaagtcgat gccatattcg acaaacatga ggtgcaggag
     4081 attaaggaga agatcctgaa ctcagattac gatgtcgaag atttcttcga gggagagttc
     4141 ttcaacttcg tgctcacaca agagggcatt gatgtgtaca atgcaatcat tggagggttc
     4201 gtgacagaga gtggcgagaa gataaagggc ctgaacgagt atatcaacct ctacaaccag
     4261 aaaaccaagc agaaactgcc taagttcaag ccactgtaca aacaagtgct ctcagatagg
     4321 gaaagcctga gcttctacgg tgaagggtat acatcagatg aagaagtgct cgaagtgttc
     4381 cgcaacaccc tcaataagaa cagtgaaatc ttctcttcaa tcaagaagct ggagaaactg
     4441 ttcaagaatt tcgatgagta ctcctctgcc ggaatctttg tgaagaatgg ccctgcaata
     4501 tccactatta gcaaagacat ctttggcgag tggaacgtta tcagggataa gtggaatgcc
     4561 gagtacgatg atattcatct caagaagaaa gccgtggtta cagagaaata cgaggatgat
     4621 agacgcaaga gctttaagaa gattggtagc ttctctctcg aacagctgca ggagtacgcc
     4681 gacgctgacc tgtcagtcgt ggagaaactc aaggagatca taatccagaa ggtggatgaa
     4741 atctacaaag tgtatggaag ctctgagaaa ctcttcgatg cagactttgt tctggagaag
     4801 agtctgaaga agaacgacgc agtggttgct atcatgaagg acctgctgga ttctgttaag
     4861 tctttcgaga attacattaa ggcattcttt ggtgaaggga aggagacaaa tagggacgag
     4921 agcttctatg gcgactttgt tctggcctac gacatcctcc tcaaggttga ccacatctat
     4981 gacgctatac ggaattacgt tacccagaag ccctatagca aagacaagtt caagctgtat
     5041 ttccagaatc cacagtttat gagggggtgg gataaagacg tggaaacaga taggagggcc
     5101 actatcctgc ggtacggcag caaatactat ctggctatca tggataagaa gtacgccaaa
     5161 tgcctccaga agatcgacaa ggacgacgtg aacggtaact acgagaagat caattacaag
     5221 ctcctgccag gacctaacaa gatgctgccc agggtgttct tctccaagaa atggatggcc
     5281 tactataacc caagcgagga cattcagaag atatacaaga atgggacatt caagaagggc
     5341 gatatgttca acctcaacga ctgccacaag ctgattgatt tcttcaagga tagcatttct
     5401 cgctatccca agtggtctaa tgcatacgat ttcaacttca gcgagactga gaagtacaaa
     5461 gacatcgctg gcttctaccg ggaggtggaa gagcaaggct ataaggtgtc attcgaatcc
     5521 gcttctaaga aggaagtgga taagctcgtg gaagagggta agctgtacat gttccagata
     5581 tacaacaaag acttcagcga taagagccac ggcactccaa acctccatac tatgtatttc
     5641 aagctgctgt ttgacgagaa caaccacgga cagattaggc tgtcaggagg cgcagaactc
     5701 ttcatgcgca gagcttcact gaagaaggag gaactcgttg tccacccagc caatagccct
     5761 atagctaata agaatccaga caatcctaag aaaaccacta ctctgtctta cgatgtgtat
     5821 aaggataaga gattctctga agatcagtac gaactgcaca tacccattgc cattaacaag
     5881 tgccctaaga acatcttcaa gattaacaca gaggttagag tgctcctgaa acacgacgat
     5941 aacccttatg ttataggcat tgctcgcgga gagagaaacc tgctgtacat cgtcgtggtg
     6001 gacggcaaag gcaacatcgt ggaacagtat agtctcaatg aaatcattaa caatttcaac
     6061 ggaatccgca ttaagaccga ctaccattct ctcctcgaca agaaggagaa agaaaggttc
     6121 gaagcaagac agaattggac aagtatagag aatatcaaag aactgaaggc tgggtacatc
     6181 tctcaggttg tgcacaagat atgtgagctg gtggagaagt acgacgctgt tatcgccctc
     6241 gcggacctga atagcggctt caagaactcc agggtgaagg tggagaagca ggtgtatcag
     6301 aagttcgaga agatgctgat cgacaagctc aactatatgg tggacaagaa atccaatcct
     6361 tgcgctactg gtggagccct gaagggctat caaatcacca ataagttcga atctttcaag
     6421 tctatgagca cccagaatgg cttcatcttc tacatacccg catggctgac atccaagatt
     6481 gatccctcta ccggatttgt taatctgctc aagactaagt acacctctat tgctgactca
     6541 aagaagttca tatcatcatt tgaccgcatc atgtacgtgc cagaagagga cctgttcgag
     6601 tttgccctgg attacaagaa tttctctcgg actgacgccg actacatcaa gaagtggaag
     6661 ctctactctt atggtaatcg gattcgcata ttccgcaatc ccaagaagaa taacgtgttc
     6721 gattgggagg aagtttgcct caccagcgct tacaaggagc tgttcaataa gtatgggatt
     6781 aactaccagc agggcgacat aagagccctg ctgtgcgaac aatctgataa ggcattctat
     6841 tcctctttca tggcactgat gtcactgatg ctgcaaatgc gcaattccat caccggaaga
     6901 acagacgtgg cctttctgat ctctcctgtc aagaactcag atggcatctt ctacgattcc
     6961 cgcaactatg aagcacagga gaatgctatc ctgcctaaga atgccgatgc aaatggagcc
     7021 tataacatcg ccagaaaggt cctctgggcc ataggacaat tcaagaaagc tgaagatgag
     7081 aagctggaca aggtgaagat cgccatttca aacaaagagt ggctcgaata tgctcagacc
     7141 tcagtgaagc atggatcacc catggtgagc aagggcgagg agctgttcac cggggtggtg
     7201 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     7261 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     7321 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     7381 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     7441 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     7501 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     7561 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     7621 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     7681 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     7741 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     7801 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     7861 atggacgagc tgtacaagtc gaattctaag ccaggattcc atatgaagcg acctgccgcc
     7921 acaaagaagg ctggacaggc taagaagaag aaacaacgcg tgcagctaag cgactacaaa
     7981 gaccatgacg gtgattataa agatcatgac atcgattaca aggatgacga tgacaagtaa
     8041 ggatccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc
     8101 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
     8161 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
     8221 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
     8281 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
     8341 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt
     8401 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg
     8461 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac
     8521 atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga
     8581 cgtggttttc ctttgaaaaa cacgatgata aggatccacc ggagcttacc atgaccgagt
     8641 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg
     8701 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg
     8761 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     8821 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     8881 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     8941 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     9001 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     9061 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     9121 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     9181 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccatcga
     9241 tagatcctaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     9301 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     9361 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     9421 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     9481 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     9541 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     9601 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     9661 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     9721 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     9781 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     9841 agatccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     9901 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     9961 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
    10021 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
    10081 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
    10141 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
    10201 atcagagagt gagaggcccg ggttaattaa ggaaagggct agatcattct tgaagacgaa
    10261 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
    10321 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
    10381 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
    10441 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
    10501 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
    10561 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
    10621 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
    10681 gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt
    10741 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
    10801 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
    10861 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
    10921 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
    10981 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
    11041 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
    11101 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
    11161 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
    11221 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
    11281 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
    11341 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
    11401 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    11461 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
    11521 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
    11581 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
    11641 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
    11701 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
    11761 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
    11821 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
    11881 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
    11941 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    12001 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    12061 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    12121 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    12181 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
    12241 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
    12301 attaatgcag caagctcatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
    12361 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
    12421 caaaaagctc cccgtggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
    12481 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
    12541 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca
    12601 tgattacgaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
    12661 aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc
    12721 atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac
    12781 tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat
    12841 gtactacaaa cttagtagt
//
