LOCUS       Exported               12877 bp ds-DNA     circular SYN 10-MAR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12877)
  AUTHORS   MINYH
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Mar 13, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12877
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     492..511
                     /label=PCDNA3.0-F2
     primer_bind     494..514
                     /label=PCDNA3.1-F
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     complement(2076..2095)
                     /label=PLKO1-R
     primer_bind     2088..2111
                     /label=U6-pll3-7
     promoter        2224..3405
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     primer_bind     complement(2296..2318)
                     /label=pCDH1-R
     primer_bind     complement(2341..2360)
                     /label=EF1A-R
     intron          2454..3396
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3353..3373
                     /label=EF-1aFwd(PEF-F)
     CDS             3418..3465
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             3472..7152
                     /codon_start=1
                     /label=dLbCas12a
                     /translation="SKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAED
                     YKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKEI
                     AKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFRNRENMFSE
                     EAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEGE
                     FFNFVLTQEGIRVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVLS
                     DRESLSFYGRGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKNG
                     PAISTISKRIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQL
                     QEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKDL
                     LDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYSK
                     DKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNGN
                     YEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKLI
                     DFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLVE
                     EGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKKE
                     ELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKIN
                     TEVRVLLKHDDNPYVIGIARGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTDY
                     HSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALADLNSGF
                     KNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQN
                     GFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALDY
                     KNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQQ
                     GDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVAFLISPVKNSDGIFYDSRNY
                     EAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTSV
                     KH"
     primer_bind     complement(3914..3957)
                     /label=D156R-R
     primer_bind     3914..3957
                     /label=D156R-F
     misc_feature    3934..3936
                     /label=D156R
     primer_bind     complement(4154..4195)
                     /label=D235R-R
     primer_bind     4154..4195
                     /label=D235R-F
     misc_feature    4171..4173
                     /label=D235R
     primer_bind     4187..4205
                     /label=7-1
     primer_bind     complement(4325..4367)
                     /label=E292R-R
     primer_bind     4325..4367
                     /label=E292R-F
     misc_feature    4342..4344
                     /label=E292R
     primer_bind     complement(4495..4538)
                     /label=D350R-R
     primer_bind     4495..4538
                     /label=D350R-F
     misc_feature    4516..4518
                     /label=D350R
     CDS             7180..7896
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             7924..7971
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7990..8055
                     /codon_start=1
                     /product="three tandem FLAG epitope tags, followed by an 
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    8062..8629
                     /label=IRES
     primer_bind     complement(8077..8100)
                     /label=PQCXIP-R (=pQC-R)
     primer_bind     complement(8102..8121)
                     /label=pIRES2-EGFP-P3
     primer_bind     complement(8151..8170)
                     /label=IRESrev
     primer_bind     8504..8524
                     /label=IRES-F
     CDS             8649..9248
                     /codon_start=1
                     /label=PuroR
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    9267..9855
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9302..9324)
                     /label=PCDNA3-4-R
     primer_bind     complement(9320..9340)
                     /label=WPRE-R
     primer_bind     complement(9343..9360)
                     /label=GIPZ sequencing primer
     CDS             complement(9738..9749)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9930..10163
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     10021..10040
                     /label=PCDNA3.0-F2
     primer_bind     10023..10043
                     /label=PCDNA3.1-F
     primer_bind     complement(10282..10300)
                     /label=pBRforEco
     primer_bind     complement(10334..10353)
                     /label=PBR322F
     primer_bind     complement(10350..10369)
                     /label=PTRG-R
     promoter        10368..10472
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10428..10446)
                     /label=BLA-Prorev
     CDS             10473..11333
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10779..10801
                     /label=AmpR-1F
     primer_bind     complement(11004..11022)
                     /label=AmpR-1R
     primer_bind     11226..11249
                     /label=PrimerBlaM1
     primer_bind     11226..11249
                     /label=Primer Bla M1
     primer_bind     complement(11431..11454)
                     /label=PrimerBlaRV
     primer_bind     complement(11431..11454)
                     /label=Primer Bla RV
     rep_origin      11504..12092
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12279..12291
                     /label=PCAMBIA2300-5
     primer_bind     12355..12374
                     /label=SV40pro-F
     primer_bind     complement(12378..12389)
                     /label=SV40pro-F
     primer_bind     12410..12427
                     /label=SV40ori-3for
     protein_bind    12503..12524
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     12533..12552
                     /label=PCAMBIA1301-F(即为P1)
     primer_bind     12534..12555
                     /label=SRprimer
     primer_bind     12536..12555
                     /label=ptarget-R
     promoter        12539..12569
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     12553..12572
                     /label=Y-M13R-48
     primer_bind     12560..12577
                     /label=PCAMBIA1300-5
     protein_bind    12577..12593
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12580..12599
                     /label=pQE30-F
     primer_bind     12582..12605
                     /label=M13R-48
     primer_bind     12582..12603
                     /label=PQ60-F
     primer_bind     12601..12617
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12601..12617
                     /label=pDONR222-R
     primer_bind     12601..12617
                     /label=M13Rev(-26)
     primer_bind     12608..12626
                     /label=M13mp18-R
     primer_bind     12652..12671
                     /label=PIRES-R
     primer_bind     12657..12676
                     /label=PCI-NEO-R
     primer_bind     12667..12686
                     /label=EBVrev
     primer_bind     12675..12694
                     /label=pCMV-R
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     2581 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     2641 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     2701 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     2761 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     2821 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     2881 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     2941 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     3001 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     3061 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     3121 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     3181 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     3241 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     3301 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     3361 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaagcgg ccgcatgaag
     3421 cgacctgccg ccacaaagaa ggctggacag gctaagaaga agaaagctag ctcaaagctg
     3481 gagaaattca ccaactgtta tagcctgtct aagaccctgc gcttcaaggc aatcccagtg
     3541 ggcaagacac aagagaacat tgacaacaaa cggctcctgg tggaggatga gaagagggct
     3601 gaagattaca agggcgttaa gaagctgctg gataggtact atctgtcatt catcaacgat
     3661 gtcctccaca gtatcaagct gaagaatctg aacaattaca tttctctgtt ccggaagaag
     3721 acacggaccg agaaggagaa caaagagctg gagaatctgg agatcaacct gaggaaagaa
     3781 atagctaagg ctttcaaagg gaacgagggt tacaagtccc tgttcaagaa agacattatc
     3841 gagactattc tgcctgagtt cctggacgat aaagatgaga tcgccctcgt caattccttc
     3901 aatgggttta ccacagcctt taccggcttc ttcaggaata gagagaatat gttctctgaa
     3961 gaggccaaat ccactagcat cgcctttcgc tgcataaacg agaacctgac taggtacatc
     4021 agcaatatgg acatctttga gaaagtcgat gccatattcg acaaacatga ggtgcaggag
     4081 attaaggaga agatcctgaa ctcagattac gatgtcgaag atttcttcga gggagagttc
     4141 ttcaacttcg tgctcacaca agagggcatt agggtgtaca atgcaatcat tggagggttc
     4201 gtgacagaga gtggcgagaa gataaagggc ctgaacgagt atatcaacct ctacaaccag
     4261 aaaaccaagc agaaactgcc taagttcaag ccactgtaca aacaagtgct ctcagatagg
     4321 gaaagcctga gcttctacgg tagggggtat acatcagatg aagaagtgct cgaagtgttc
     4381 cgcaacaccc tcaataagaa cagtgaaatc ttctcttcaa tcaagaagct ggagaaactg
     4441 ttcaagaatt tcgatgagta ctcctctgcc ggaatctttg tgaagaatgg ccctgcaata
     4501 tccactatta gcaaaaggat ctttggcgag tggaacgtta tcagggataa gtggaatgcc
     4561 gagtacgatg atattcatct caagaagaaa gccgtggtta cagagaaata cgaggatgat
     4621 agacgcaaga gctttaagaa gattggtagc ttctctctcg aacagctgca ggagtacgcc
     4681 gacgctgacc tgtcagtcgt ggagaaactc aaggagatca taatccagaa ggtggatgaa
     4741 atctacaaag tgtatggaag ctctgagaaa ctcttcgatg cagactttgt tctggagaag
     4801 agtctgaaga agaacgacgc agtggttgct atcatgaagg acctgctgga ttctgttaag
     4861 tctttcgaga attacattaa ggcattcttt ggtgaaggga aggagacaaa tagggacgag
     4921 agcttctatg gcgactttgt tctggcctac gacatcctcc tcaaggttga ccacatctat
     4981 gacgctatac ggaattacgt tacccagaag ccctatagca aagacaagtt caagctgtat
     5041 ttccagaatc cacagtttat gggtgggtgg gataaagaca aagaaacaga ttacagggcc
     5101 actatcctgc ggtacggcag caaatactat ctggctatca tggataagaa gtacgccaaa
     5161 tgcctccaga agatcgacaa ggacgacgtg aacggtaact acgagaagat caattacaag
     5221 ctcctgccag gacctaacaa gatgctgccc aaggtgttct tctccaagaa atggatggcc
     5281 tactataacc caagcgagga cattcagaag atatacaaga atgggacatt caagaagggc
     5341 gatatgttca acctcaacga ctgccacaag ctgattgatt tcttcaagga tagcatttct
     5401 cgctatccca agtggtctaa tgcatacgat ttcaacttca gcgagactga gaagtacaaa
     5461 gacatcgctg gcttctaccg ggaggtggaa gagcaaggct ataaggtgtc attcgaatcc
     5521 gcttctaaga aggaagtgga taagctcgtg gaagagggta agctgtacat gttccagata
     5581 tacaacaaag acttcagcga taagagccac ggcactccaa acctccatac tatgtatttc
     5641 aagctgctgt ttgacgagaa caaccacgga cagattaggc tgtcaggagg cgcagaactc
     5701 ttcatgcgca gagcttcact gaagaaggag gaactcgttg tccacccagc caatagccct
     5761 atagccaata agaatccaga caatcctaag aaaaccacta ctctgtctta cgatgtgtat
     5821 aaggataaga gattctctga agatcagtac gaactgcaca tacccattgc cattaacaag
     5881 tgccctaaga acatcttcaa gattaacaca gaggttagag tgctcctgaa acacgacgat
     5941 aacccttatg ttataggcat tgctcgcgga gagagaaacc tgctgtacat cgtcgtggtg
     6001 gacggcaaag gcaacatcgt ggaacagtac agtctcaatg aaatcattaa caatttcaac
     6061 ggaatccgca ttaagaccga ctaccattct ctcctcgaca agaaggagaa agaaaggttc
     6121 gaagcaagac agaattggac aagtatagag aatatcaaag aactgaaggc tgggtacatc
     6181 tctcaggttg tgcacaagat atgtgagctg gtggagaagt acgacgctgt tatcgccctc
     6241 gcggacctga atagcggctt caagaactcc agggtgaagg tggagaagca ggtgtatcag
     6301 aagttcgaga agatgctgat cgacaagctc aactatatgg tggacaagaa atccaatcct
     6361 tgcgctactg gtggagccct gaagggctat caaatcacca ataagttcga atctttcaag
     6421 tctatgagca cccagaatgg cttcatcttc tacatacccg catggctgac atccaagatt
     6481 gatccctcta ccggatttgt taatctgctc aagactaagt acacctctat tgctgactca
     6541 aagaagttca tatcatcatt tgaccgcatc atgtacgtgc cagaagagga cctgttcgag
     6601 tttgccctgg attacaagaa tttctctcgg actgacgccg actacatcaa gaagtggaag
     6661 ctctactctt atggtaatcg gattcgcata ttccgcaatc ccaagaagaa taacgtgttc
     6721 gattgggagg aagtttgcct caccagcgct tacaaggagc tgttcaataa gtatgggatt
     6781 aactaccagc agggcgacat aagagccctg ctgtgcgaac aatctgataa ggcattctat
     6841 tcctctttca tggcactgat gtcactgatg ctgcaaatgc gcaattccat caccggaaga
     6901 acagacgtgg cctttctgat ctctcctgtc aagaactcag atggcatctt ctacgattcc
     6961 cgcaactatg aagcacagga gaatgctatc ctgcctaaga atgccgatgc aaatggagcc
     7021 tataacatcg ccagaaaggt cctctgggcc ataggacaat tcaagaaagc tgaagatgag
     7081 aagctggaca aggtgaagat cgccatttca aacaaagagt ggctcgaata tgctcagacc
     7141 tcagtgaagc atagtctaga ggcgctaccg gtcgccacca tggtgagcaa gggcgaggag
     7201 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     7261 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     7321 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     7381 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     7441 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     7501 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     7561 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     7621 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     7681 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     7741 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     7801 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     7861 gccgggatca ctctcggcat ggacgagctg tacaagtcga attctaagcc aggattccat
     7921 atgaagcgac ctgccgccac aaagaaggct ggacaggcta agaagaagaa acaacgcgtg
     7981 cagctaagcg actacaaaga ccatgacggt gattataaag atcatgacat cgattacaag
     8041 gatgacgatg acaagtaagg atccctcccc cccccctaac gttactggcc gaagccgctt
     8101 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     8161 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     8221 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     8281 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     8341 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     8401 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     8461 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     8521 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     8581 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataag gatccaccgg
     8641 agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     8701 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     8761 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     8821 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     8881 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     8941 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     9001 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     9061 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     9121 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     9181 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     9241 gtgcctgacg cccatcgata gatcctaatc aacctctgga ttacaaaatt tgtgaaagat
     9301 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     9361 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     9421 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     9481 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     9541 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     9601 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     9661 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     9721 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     9781 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     9841 gggccgcctc cccgcctgag atcctttaag accaatgact tacaaggcag ctgtagatct
     9901 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     9961 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
    10021 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
    10081 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
    10141 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
    10201 acttgcaaag aaatgaatat cagagagtga gaggcccggg ttaattaagg aaagggctag
    10261 atcattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
    10321 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
    10381 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
    10441 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
    10501 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
    10561 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
    10621 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
    10681 ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg
    10741 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
    10801 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
    10861 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
    10921 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
    10981 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
    11041 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
    11101 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
    11161 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
    11221 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
    11281 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
    11341 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
    11401 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
    11461 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
    11521 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
    11581 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
    11641 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
    11701 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
    11761 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
    11821 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
    11881 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
    11941 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
    12001 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
    12061 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
    12121 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
    12181 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
    12241 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
    12301 ccgcgcgttg gccgattcat taatgcagca agctcatggc tgactaattt tttttattta
    12361 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
    12421 tggaggccta ggcttttgca aaaagctccc cgtggcacga caggtttccc gactggaaag
    12481 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
    12541 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
    12601 caggaaacag ctatgacatg attacgaatt tcacaaataa agcatttttt tcactgcatt
    12661 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa
    12721 ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt
    12781 acctgtggtt tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa
    12841 attgtatttg ttaaatatgt actacaaact tagtagt
//
