LOCUS       Exported               13093 bp ds-DNA     circular SYN 25-NOV-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13093)
  AUTHORS   MINYH
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Mar 13, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13093
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     492..511
                     /label=PCDNA3.0-F2
     primer_bind     494..514
                     /label=PCDNA3.1-F
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     complement(2076..2095)
                     /label=PLKO1-R
     primer_bind     2088..2111
                     /label=U6-pll3-7
     promoter        2224..3405
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     primer_bind     complement(2296..2318)
                     /label=pCDH1-R
     primer_bind     complement(2341..2360)
                     /label=EF1A-R
     intron          2454..3396
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3353..3373
                     /label=EF-1aFwd(PEF-F)
     CDS             3418..3465
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     3450..3497
                     /label=dFnCas12a-NheI-F
     CDS             3472..7368
                     /codon_start=1
                     /label=FnCpf1
                     /translation="SIYQEFVNKYSLSKTLRFELIPQGKTLENIKARGLILDDEKRAKD
                     YKKAKQIIDKYHQFFIEEILSSVCISEDLLQNYSDVYFKLKKSDDDNLQKDFKSAKDTI
                     KKQISEYIKDSEKFKNLFNQNLIDAKKGQESDLILWLKQSKDNGIELFKANSDITDIDE
                     ALEIIKSFKGWTTYFKGFHRNRKNVYSSNDIPTSIIYRIVDDNLPKFLENKAKYESLKD
                     KAPEAINYEQIKKDLAEELTFDIDYKTSEVNQRVFSLDEVFEIANFNNYLNQSGIRKFN
                     TIIGGKFVNGENTKRKGINEYINLYSQQINDKTLKKYKMSVLFKQILSDTESKSFVIRK
                     LEDDSDVVTTMQSFYEQIAAFKTVEEKSIKETLSLLFDDLKAQKLDLSKIYFKNDKSLT
                     DLSQQVFDRYSVIGTAVLEYITQQIAPKNLDNPSKKEQELIAKKTEKAKYLSLETIKLA
                     LEEFNKHRDIDKQCRFEEILANFAAIPMIFDEIAQNKDNLAQISIKYQNQGKKDLLQAS
                     AEDDVKAIKDLLDQTNNLLHKLKIFHISQSEDKANILDKDEHFYLVFEECYFELANIVP
                     LYNKIRNYITQKPYSDEKFKLNFENSTLANGWDKNKEPDNTAILFIKDDKYYLGVMNKK
                     NNKIFDDKAIKENKGEGYKKIVYKLLPGANKMLPKVFFSAKSIKFYNPSEDILRIRNHS
                     THTKNGSPQKGYEKFEFNIEDCRKFIDFYKQSISKHPEWKDFGFRFSDTQRYNSIDEFY
                     REVENQGYKLTFENISESYIDSVVNQGKLYLFQIYNKDFSAYSKGRPNLHTLYWKALFD
                     ERNLQDVVYKLNGEAELFYRKQSIPKKITHPAKEAIANKNKDNPKKESVFEYDLIKDKR
                     FTEDKFFFHCPITINFKSSGANKFNDEINLLLKEKANDVHILSIARGERHLAYYTLVDG
                     KGNIIKQDTFNIIGNDRMKTNYHDKLAAIEKDRDSARKDWKKINNIKEMKEGYLSQVVH
                     EIAKLVIEYNAIVVFADLNFGFKRGRFKVEKQVYQKLEKMLIEKLNYLVFKDNEFDKTG
                     GVLRAYQLTAPFETFKKMGKQTGIIYYVPAGFTSKICPVTGFVNQLYPKYESVSKSQEF
                     FSKFDKICYNLDKGYFEFSFDYKNFGDKAAKGKWTIASFGSRLINFRNSDKNHNWDTRE
                     VYPTKELEKLLKDYSIEYGHGECIKAAICGESDKKFFAKLTSVLNTILQMRNSKTGTEL
                     DYLISPVADVNGNFFDSRQAPKNMPQDAAANGAYHIGLKGLMLLGRIKNNQEGKKLNLV
                     IKNEEYFEFVQNRNN"
     primer_bind     complement(4007..4034)
                     /label=Fn-hE184R-R
     primer_bind     4007..4034
                     /label=Fn-hE184R-F
     misc_feature    4018..4020
                     /label=hE184R
     primer_bind     4206..4224
                     /label=15-1
     primer_bind     complement(4294..4322)
                     /label=Fn-hT279R-R
     primer_bind     4294..4322
                     /label=Fn-hT279R-F
     misc_feature    4303..4305
                     /label=hT279R
     primer_bind     complement(4474..4501)
                     /label=Fn-hD340R-R
     primer_bind     4474..4501
                     /label=Fn-hD340R-F
     misc_feature    4486..4488
                     /label=hD340R
     primer_bind     complement(4681..4708)
                     /label=Fn-hD409R-R
     primer_bind     4681..4708
                     /label=Fn-hD409R-F
     misc_feature    4693..4695
                     /label=hD409R
     primer_bind     5026..5044
                     /label=15-2
     primer_bind     complement(5266..5324)
                     /label=FnRVR-R
     primer_bind     5278..5336
                     /label=FnRVR-F
     primer_bind     5853..5871
                     /label=15-3
     primer_bind     6677..6695
                     /label=15-4
     primer_bind     complement(7345..7403)
                     /label=FndCas12a-NcoI-R
     CDS             7396..8112
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7441..7462)
                     /label=pEGFP-N3
     primer_bind     complement(7447..7462)
                     /label=EGFP-Nrev
     primer_bind     complement(7472..7490)
                     /label=pRECEIVER-M03R
     primer_bind     7532..7544
                     /label=FR69 pTV00
     primer_bind     7637..7656
                     /label=EGFP-F
     primer_bind     complement(7861..7880)
                     /label=EGFP-R
     primer_bind     8002..8022
                     /label=EGFP-Cfor
     primer_bind     8049..8070
                     /label=pEGFP-C5
     CDS             8140..8187
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             8206..8271
                     /codon_start=1
                     /product="three tandem FLAG epitope tags, followed by an 
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    8278..8845
                     /label=IRES
     primer_bind     complement(8293..8316)
                     /label=PQCXIP-R (=pQC-R)
     primer_bind     complement(8318..8337)
                     /label=pIRES2-EGFP-P3
     primer_bind     complement(8367..8386)
                     /label=IRESrev
     primer_bind     8720..8740
                     /label=IRES-F
     CDS             8865..9464
                     /codon_start=1
                     /label=PuroR
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    9483..10071
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9518..9540)
                     /label=PCDNA3-4-R
     primer_bind     complement(9536..9556)
                     /label=WPRE-R
     primer_bind     complement(9559..9576)
                     /label=GIPZ sequencing primer
     CDS             complement(9954..9965)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10146..10379
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     10237..10256
                     /label=PCDNA3.0-F2
     primer_bind     10239..10259
                     /label=PCDNA3.1-F
     primer_bind     complement(10498..10516)
                     /label=pBRforEco
     primer_bind     complement(10550..10569)
                     /label=PBR322F
     primer_bind     complement(10566..10585)
                     /label=PTRG-R
     promoter        10584..10688
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10644..10662)
                     /label=BLA-Prorev
     CDS             10689..11549
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10995..11017
                     /label=AmpR-1F
     primer_bind     complement(11220..11238)
                     /label=AmpR-1R
     primer_bind     11442..11465
                     /label=Primer Bla M1
     primer_bind     11442..11465
                     /label=PrimerBlaM1
     primer_bind     complement(11647..11670)
                     /label=Primer Bla RV
     primer_bind     complement(11647..11670)
                     /label=PrimerBlaRV
     rep_origin      11720..12308
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12495..12507
                     /label=PCAMBIA2300-5
     primer_bind     12571..12590
                     /label=SV40pro-F
     primer_bind     complement(12594..12605)
                     /label=SV40pro-F
     primer_bind     12626..12643
                     /label=SV40ori-3for
     protein_bind    12719..12740
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     12749..12768
                     /label=PCAMBIA1301-F(即为P1)
     primer_bind     12750..12771
                     /label=SRprimer
     primer_bind     12752..12771
                     /label=ptarget-R
     promoter        12755..12785
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     12769..12788
                     /label=Y-M13R-48
     primer_bind     12776..12793
                     /label=PCAMBIA1300-5
     protein_bind    12793..12809
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12796..12815
                     /label=pQE30-F
     primer_bind     12798..12821
                     /label=M13R-48
     primer_bind     12798..12819
                     /label=PQ60-F
     primer_bind     12817..12833
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12817..12833
                     /label=M13Rev(-26)
     primer_bind     12817..12833
                     /label=pDONR222-R
     primer_bind     12824..12842
                     /label=M13mp18-R
     primer_bind     12868..12887
                     /label=PIRES-R
     primer_bind     12873..12892
                     /label=PCI-NEO-R
     primer_bind     12883..12902
                     /label=EBVrev
     primer_bind     12891..12910
                     /label=pCMV-R
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     2581 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     2641 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     2701 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     2761 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     2821 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     2881 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     2941 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     3001 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     3061 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     3121 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     3181 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     3241 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     3301 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     3361 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaagcgg ccgcatgaag
     3421 cgacctgccg ccacaaagaa ggctggacag gctaagaaga agaaagctag cagcatctac
     3481 caggagttcg tcaacaagta ttcactgagt aagacactgc ggttcgagct gatcccacag
     3541 ggcaagacac tggagaacat caaggcccga ggcctgattc tggacgatga gaagcgggca
     3601 aaagactata agaaagccaa gcagatcatt gataaatacc accagttctt tatcgaggaa
     3661 attctgagct ccgtgtgcat cagtgaggat ctgctgcaga attactcaga cgtgtacttc
     3721 aagctgaaga agagcgacga tgacaacctg cagaaggact tcaagtccgc caaggacacc
     3781 atcaagaaac agattagcga gtacatcaag gactccgaaa agtttaaaaa tctgttcaac
     3841 cagaatctga tcgatgctaa gaaaggccag gagtccgacc tgatcctgtg gctgaaacag
     3901 tctaaggaca atgggattga actgttcaag gctaactccg atatcactga tattgacgag
     3961 gcactggaaa tcatcaagag cttcaaggga tggaccacat actttaaagg cttccacaga
     4021 aaccgcaaga acgtgtactc cagcaacgac attcctacct ccatcatcta ccgaatcgtc
     4081 gatgacaatc tgccaaagtt cctggagaac aaggccaaat atgaatctct gaaggacaaa
     4141 gctcccgagg caattaatta cgaacagatc aagaaagatc tggctgagga actgacattc
     4201 gatatcgact ataagactag cgaggtgaac cagagggtct tttccctgga cgaggtgttt
     4261 gaaatcgcca atttcaacaa ttacctgaac cagtccggca ttagaaaatt caataccatc
     4321 attggcggga agtttgtgaa cggggagaat accaagcgca agggaattaa cgaatacatc
     4381 aatctgtata gccagcagat caacgacaaa actctgaaga aatacaagat gtctgtgctg
     4441 ttcaaacaga tcctgagtga taccgagtcc aagtcttttg tcattagaaa actggaagat
     4501 gactcagacg tggtcactac catgcagagc ttttatgagc agatcgccgc tttcaagaca
     4561 gtggaggaaa aatctattaa ggaaactctg agtctgctgt tcgatgacct gaaagcccag
     4621 aagctggacc tgagtaagat ctacttcaaa aacgataaga gtctgacaga cctgtcacag
     4681 caggtgtttg atagatattc cgtgattggg accgccgtcc tggagtacat tacacagcag
     4741 atcgctccaa agaacctgga taatccctct aagaaagagc aggaactgat cgctaagaaa
     4801 accgagaagg caaaatatct gagtctggaa acaattaagc tggcactgga ggagttcaac
     4861 aagcacaggg atattgacaa acagtgccgc tttgaggaaa tcctggccaa cttcgcagcc
     4921 atccccatga tttttgatga gatcgcccag aacaaagaca atctggctca gatcagtatt
     4981 aagtaccaga accagggcaa gaaagacctg ctgcaggctt cagcagaaga tgacgtgaaa
     5041 gccatcaagg atctgctgga ccagaccaac aatctgctgc acaagctgaa aatcttccat
     5101 attagtcagt cagaggataa ggctaatatc ctggataaag acgaacactt ctacctggtg
     5161 ttcgaggaat gttacttcga gctggcaaac attgtccccc tgtataacaa gattaggaac
     5221 tacatcacac agaagcctta ctctgacgag aagtttaaac tgaacttcga aaatagtacc
     5281 ctggccaacg ggtgggataa gaacaaggag cctgacaaca cagctatcct gttcatcaag
     5341 gatgacaagt actatctggg agtgatgaat aagaaaaaca ataagatctt cgatgacaaa
     5401 gccattaagg agaacaaagg ggaaggatac aagaaaatcg tgtataagct gctgcccggc
     5461 gcaaataaga tgctgcctaa ggtgttcttc agcgccaaga gtatcaaatt ctacaaccca
     5521 tccgaggaca tcctgcggat tagaaatcac tcaacacata ctaagaacgg gagcccccag
     5581 aagggatatg agaaatttga gttcaacatc gaggattgca ggaagtttat tgacttctac
     5641 aagcagagca tctccaaaca ccctgaatgg aaggattttg gcttccggtt ttccgacaca
     5701 cagagatata actctatcga cgagttctac cgcgaggtgg aaaatcaggg gtataagctg
     5761 acttttgaga acatttctga aagttacatc gacagcgtgg tcaatcaggg aaagctgtac
     5821 ctgttccaga tctataacaa agatttttca gcatacagca agggcagacc aaacctgcat
     5881 acactgtact ggaaggccct gttcgatgag aggaatctgc aggacgtggt ctataaactg
     5941 aacggagagg ccgaactgtt ttaccggaag cagtctattc ctaagaaaat cactcaccca
     6001 gctaaggagg ccatcgctaa caagaacaag gacaatccta agaaagagag cgtgttcgaa
     6061 tacgatctga ttaaggacaa gcggttcacc gaagataagt tctttttcca ttgtccaatc
     6121 accattaact tcaagtcaag cggcgctaac aagttcaacg acgagatcaa tctgctgctg
     6181 aaggaaaaag caaacgatgt gcacatcctg agcattgccc gaggagagcg gcatctggcc
     6241 tactataccc tggtggatgg caaagggaat atcattaagc aggatacatt caacatcatt
     6301 ggcaatgacc ggatgaaaac caactaccac gataaactgg ctgcaatcga gaaggataga
     6361 gactcagcta ggaaggactg gaagaaaatc aacaacatta aggagatgaa ggaaggctat
     6421 ctgagccagg tggtccatga gattgcaaag ctggtcatcg aatacaatgc cattgtggtg
     6481 ttcgcggatc tgaacttcgg ctttaagagg gggcgcttta aggtggaaaa acaggtctat
     6541 cagaagctgg agaaaatgct gatcgaaaag ctgaattacc tggtgtttaa agataacgag
     6601 ttcgacaaga ccggaggcgt cctgagagcc taccagctga cagctccctt tgaaactttc
     6661 aagaaaatgg gaaaacagac aggcatcatc tactatgtgc cagccggatt cacttccaag
     6721 atctgccccg tgaccggctt tgtcaaccag ctgtacccta aatatgagtc agtgagcaag
     6781 tcccaggaat ttttcagcaa gttcgataag atctgttata atctggacaa ggggtacttc
     6841 gagttttcct tcgattacaa gaacttcggc gacaaggccg ctaaggggaa atggaccatt
     6901 gcctccttcg gatctcgcct gatcaacttt cgaaattccg ataaaaacca caattgggac
     6961 actagggagg tgtacccaac caaggagctg gaaaagctgc tgaaagacta ctctatcgag
     7021 tatggacatg gcgaatgcat caaggcagcc atctgtggcg agagtgataa gaaatttttc
     7081 gccaagctga cctcagtgct gaatacaatc ctgcagatgc ggaactcaaa gaccgggaca
     7141 gaactggact atctgattag ccccgtggct gatgtcaacg gaaacttctt cgacagcaga
     7201 caggcaccca aaaatatgcc tcaggatgca gccgccaacg gggcctacca catcgggctg
     7261 aagggactga tgctgctggg ccggatcaag aacaatcagg aggggaagaa gctgaacctg
     7321 gtcattaaga acgaggaata cttcgagttt gtccagaata gaaataacag tctagaggcg
     7381 ctaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     7441 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     7501 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     7561 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     7621 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     7681 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     7741 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     7801 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     7861 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     7921 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     7981 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     8041 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     8101 gagctgtaca agtcgaattc taagccagga ttccatatga agcgacctgc cgccacaaag
     8161 aaggctggac aggctaagaa gaagaaacaa cgcgtgcagc taagcgacta caaagaccat
     8221 gacggtgatt ataaagatca tgacatcgat tacaaggatg acgatgacaa gtaaggatcc
     8281 ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
     8341 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
     8401 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
     8461 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
     8521 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
     8581 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
     8641 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
     8701 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
     8761 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
     8821 tttcctttga aaaacacgat gataaggatc caccggagct taccatgacc gagtacaagc
     8881 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     8941 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     9001 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     9061 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     9121 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     9181 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     9241 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     9301 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     9361 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     9421 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgccca tcgatagatc
     9481 ctaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     9541 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     9601 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     9661 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     9721 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     9781 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     9841 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     9901 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     9961 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
    10021 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctgagatcc
    10081 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
    10141 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
    10201 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
    10261 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
    10321 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
    10381 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
    10441 gagtgagagg cccgggttaa ttaaggaaag ggctagatca ttcttgaaga cgaaagggcc
    10501 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
    10561 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
    10621 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
    10681 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
    10741 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
    10801 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
    10861 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
    10921 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
    10981 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
    11041 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
    11101 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
    11161 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    11221 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    11281 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    11341 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    11401 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    11461 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    11521 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    11581 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    11641 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    11701 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    11761 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    11821 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    11881 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    11941 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    12001 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    12061 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    12121 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    12181 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    12241 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    12301 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    12361 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    12421 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    12481 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    12541 gcagcaagct catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
    12601 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
    12661 gctccccgtg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
    12721 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
    12781 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta
    12841 cgaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
    12901 tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca
    12961 aacttcccac cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa
    13021 cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta
    13081 caaacttagt agt
//
