LOCUS       Exported                9649 bp ds-DNA     circular SYN 11-MAR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9649)
  AUTHORS   Triple Threat
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Mar 13, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9649
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     492..511
                     /label=PCDNA3.0-F2
     primer_bind     494..514
                     /label=PCDNA3.1-F
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     complement(2076..2095)
                     /label=PLKO1-R
     primer_bind     2088..2111
                     /label=U6-pll3-7
     primer_bind     2189..2241
                     /label=CAG-F
     promoter        2217..3935
                     /label=CAG promoter
                     /label=CAG
                     /note="vntifkey=29"
     primer_bind     complement(2381..2401)
                     /label=CAGR2
     primer_bind     3216..3226
                     /label=PJET1-2-F
     primer_bind     3216..3226
                     /label=PLB-F
     primer_bind     3812..3829
                     /label=PPY-F
     primer_bind     3852..3867
                     /label=PMlink-F
     primer_bind     3895..3914
                     /label=pCAG-F
     misc_feature    3971..3991
                     /label=DR+1
     misc_feature    4012..4032
                     /label=DR+1
     misc_feature    4053..4073
                     /label=DR+1
     misc_feature    4094..4114
                     /label=DR+1
     misc_feature    4135..4155
                     /label=DR+1
     misc_feature    4176..4196
                     /label=DR+1
     misc_feature    4217..4237
                     /label=DR+1
     misc_feature    4258..4278
                     /label=DR+1
     misc_feature    4299..4319
                     /label=DR+1
     misc_feature    4340..4360
                     /label=DR+1
     misc_feature    4381..4401
                     /label=DR+1
     misc_feature    4422..4442
                     /label=DR+1
     misc_feature    4463..4483
                     /label=DR+1
     misc_feature    4504..4524
                     /label=DR+1
     misc_feature    4545..4565
                     /label=DR+1
     misc_feature    4586..4606
                     /label=DR+1
     misc_feature    4627..4647
                     /label=DR+1
     misc_feature    4668..4688
                     /label=DR+1
     misc_feature    4709..4729
                     /label=DR+1
     primer_bind     4730..4749
                     /label=18-1
     misc_feature    4750..4770
                     /label=DR+1
     misc_feature    4791..4811
                     /label=DR+1
     misc_feature    4832..4852
                     /label=DR+1
     misc_feature    4873..4893
                     /label=DR+1
     misc_feature    4914..4934
                     /label=DR+1
     misc_feature    4955..4975
                     /label=DR+1
     misc_feature    4996..5016
                     /label=DR+1
     misc_feature    5037..5057
                     /label=DR+1
     misc_feature    5078..5098
                     /label=DR+1
     misc_feature    5119..5139
                     /label=DR+1
     misc_feature    5160..5180
                     /label=DR+1
     misc_feature    5201..5221
                     /label=DR+1
     misc_feature    5242..5262
                     /label=DR+1
     misc_feature    5283..5303
                     /label=DR+1
     misc_feature    5324..5344
                     /label=DR+1
     misc_feature    5365..5385
                     /label=DR+1
     misc_feature    5406..5426
                     /label=DR+1
     misc_feature    5447..5467
                     /label=DR+1
     misc_feature    5488..5508
                     /label=DR+1
     misc_feature    5529..5549
                     /label=DR+1
     misc_feature    5570..5590
                     /label=DR+1
     misc_feature    5611..5631
                     /label=DR+1
     misc_feature    5652..5672
                     /label=DR+1
     misc_feature    5693..5713
                     /label=DR+1
     misc_feature    5734..5754
                     /label=DR+1
     misc_feature    5775..5795
                     /label=DR+1
     misc_feature    5816..5836
                     /label=DR+1
     misc_feature    5857..5877
                     /label=DR+1
     misc_feature    5898..5918
                     /label=DR+1
     misc_feature    5939..5959
                     /label=DR+1
     misc_feature    5980..6000
                     /label=DR+1
     misc_feature    6039..6627
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6074..6096)
                     /label=PCDNA3-4-R
     primer_bind     complement(6092..6112)
                     /label=WPRE-R
     primer_bind     complement(6115..6132)
                     /label=GIPZ sequencing primer
     primer_bind     complement(6380..6389)
                     /label=24XCCAT01-R
     CDS             complement(6510..6521)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6702..6935
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     6793..6812
                     /label=PCDNA3.0-F2
     primer_bind     6795..6815
                     /label=PCDNA3.1-F
     primer_bind     complement(7054..7072)
                     /label=pBRforEco
     primer_bind     complement(7106..7125)
                     /label=PBR322F
     primer_bind     complement(7122..7141)
                     /label=PTRG-R
     promoter        7140..7244
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7200..7218)
                     /label=BLA-Prorev
     CDS             7245..8105
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7551..7573
                     /label=AmpR-1F
     primer_bind     complement(7776..7794)
                     /label=AmpR-1R
     primer_bind     7998..8021
                     /label=Primer Bla M1
     primer_bind     7998..8021
                     /label=PrimerBlaM1
     primer_bind     complement(8203..8226)
                     /label=Primer Bla RV
     primer_bind     complement(8203..8226)
                     /label=PrimerBlaRV
     rep_origin      8276..8864
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9051..9063
                     /label=PCAMBIA2300-5
     primer_bind     9127..9146
                     /label=SV40pro-F
     primer_bind     complement(9150..9161)
                     /label=SV40pro-F
     primer_bind     9182..9199
                     /label=SV40ori-3for
     protein_bind    9275..9296
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     9305..9324
                     /label=PCAMBIA1301-F(即为P1)
     primer_bind     9306..9327
                     /label=SRprimer
     primer_bind     9308..9327
                     /label=ptarget-R
     promoter        9311..9341
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     9325..9344
                     /label=Y-M13R-48
     primer_bind     9332..9349
                     /label=PCAMBIA1300-5
     protein_bind    9349..9365
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9352..9371
                     /label=pQE30-F
     primer_bind     9354..9377
                     /label=M13R-48
     primer_bind     9354..9375
                     /label=PQ60-F
     primer_bind     9373..9389
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9373..9389
                     /label=M13Rev(-26)
     primer_bind     9373..9389
                     /label=pDONR222-R
     primer_bind     9380..9398
                     /label=M13mp18-R
     primer_bind     9424..9443
                     /label=PIRES-R
     primer_bind     9429..9448
                     /label=PCI-NEO-R
     primer_bind     9439..9458
                     /label=EBVrev
     primer_bind     9447..9466
                     /label=pCMV-R
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggaca
     2221 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     2281 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     2341 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     2401 ccattgacgt caatgggtgg actatttacg gtaaactgcc cacttggcag tacatcaagt
     2461 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     2521 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     2581 catcgctatt accatgggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc
     2641 ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg
     2701 gggcgggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg
     2761 gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
     2821 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc
     2881 gctgcgttgc cttcgccccg tgccccgctc cgcgccgcct cgcgccgccc gccccggctc
     2941 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt
     3001 aattagcgct tggtttaatg acggctcgtt tcttttctgt ggctgcgtga aagccttaaa
     3061 gggctccggg agggcccttt gtgcgggggg gagcggctcg gggggtgcgt gcgtgtgtgt
     3121 gtgcgtgggg agcgccgcgt gcggcccgcg ctgcccggcg gctgtgagcg ctgcgggcgc
     3181 ggcgcggggc tttgtgcgct ccgcgtgtgc gcgaggggag cgcggccggg ggcggtgccc
     3241 cgcggtgcgg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg
     3301 gtgagcaggg ggtgtgggcg cggcggtcgg gctgtaaccc ccccctgcac ccccctcccc
     3361 gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt ggcgcggggc
     3421 tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg gggccgcctc
     3481 gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag
     3541 gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc
     3601 ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg
     3661 ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg
     3721 cgtcgccgcg ccgccgtccc cttctccatc tccagcctcg gggctgtccg cagggggacg
     3781 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct
     3841 ctagtgcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg
     3901 tgctggttat tgtgctgtct catcattttg gcaaagggcg gccgcatctt gtggaaagga
     3961 cgaaacaccg taatttctac taagtgtaga tgccagttga gttgagccga ttaatttcta
     4021 ctaagtgtag attgtggccg gtggacgttt ggtaatttct actaagtgta gatggaacca
     4081 agcgcgtctc cattaatttc tactaagtgt agatttctgg cgcggggggt cctctaattt
     4141 ctactaagtg tagatcgagc tcgggtggga gtccctaatt tctactaagt gtagatgcag
     4201 cgcccggtga tttgcttaat ttctactaag tgtagatact ccacgccttc ggagacctaa
     4261 tttctactaa gtgtagatca agccctcggg aaggttgtta atttctacta agtgtagatt
     4321 gagaggcctt tcggacgttt aatttctact aagtgtagat attgcagcgc cttcgcgccg
     4381 taatttctac taagtgtaga ttgtaatgaa tgccccgcgc gtaatttcta ctaagtgtag
     4441 attttctcct cgccggcccg agtaatttct actaagtgta gatatcgctg cctccgccgc
     4501 gcataatttc tactaagtgt agatcagccg tcatcttcgc cctataattt ctactaagtg
     4561 tagatcacct caacaaagtg tattctaatt tctactaagt gtagatggag agaagactac
     4621 cgtatctaat ttctactaag tgtagatccg atacacggat agtggtctaa tttctactaa
     4681 gtgtagatga aagccttcca gggcagcgta atttctacta agtgtagatt gctccattta
     4741 ctcgagttat aatttctact aagtgtagat cagttactcc ggcctagtga taatttctac
     4801 taagtgtaga tgccactgag tcccccgtct ctaatttcta ctaagtgtag attgggaatc
     4861 catagcatac cgtaatttct actaagtgta gatcaatgaa cacgattagc tgctaatttc
     4921 tactaagtgt agatcgtctc ttccgtagcc cgggtaattt ctactaagtg tagatgtgac
     4981 ctcctgtctg gtctgtaatt tctactaagt gtagatcctc ctgttacagg cagtagtaat
     5041 ttctactaag tgtagattct ggataccctg gttttactaa tttctactaa gtgtagatac
     5101 aattgtggcc tcgcagtgta atttctacta agtgtagatc tcatgctgcc gaatgtgtgt
     5161 aatttctact aagtgtagat gtaattgagg cgcagcgttg taatttctac taagtgtaga
     5221 tcaccctgtt tgtcgtcagc gtaatttcta ctaagtgtag atccagtcgt gttacgcaac
     5281 aataatttct actaagtgta gatgtgctgg ggcttgagac gactaatttc tactaagtgt
     5341 agatggcaag cctgtgagtc actgtaattt ctactaagtg tagataacgc actcttgttc
     5401 gtgattaatt tctactaagt gtagataaga caactcgacc ttcaagtaat ttctactaag
     5461 tgtagatgtt tacccttaca cttgcgataa tttctactaa gtgtagatta tccaggttgg
     5521 acttgtatta atttctacta agtgtagatg gacgtaatat agacgtaaat aatttctact
     5581 aagtgtagat accgtgggca gattatgcaa taatttctac taagtgtaga tgatagagaa
     5641 atcatccaag ctaatttcta ctaagtgtag atgcatcata ccctacaccg cttaatttct
     5701 actaagtgta gatcagtagg acagcagcgt ctataatttc tactaagtgt agataactct
     5761 ggagatcagt gttctaattt ctactaagtg tagatatcac tatcaatact ggagctaatt
     5821 tctactaagt gtagattgaa cctcaccgca gaacagtaat ttctactaag tgtagatccc
     5881 ccagaccaga tatgacctaa tttctactaa gtgtagatcc ctaaatgaaa cgcacgttta
     5941 atttctacta agtgtagata gacacgacaa gacaaatcat aatttctact aagtgtagat
     6001 ctgccttgtc aggatgggac cgagaatcga tagatcctaa tcaacctctg gattacaaaa
     6061 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     6121 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     6181 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     6241 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     6301 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     6361 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     6421 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     6481 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     6541 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     6601 ggatctccct ttgggccgcc tccccgcctg agatccttta agaccaatga cttacaaggc
     6661 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     6721 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     6781 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     6841 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     6901 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     6961 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcccg ggttaattaa
     7021 ggaaagggct agatcattct tgaagacgaa agggcctcgt gatacgccta tttttatagg
     7081 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     7141 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     7201 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     7261 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     7321 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     7381 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     7441 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc
     7501 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     7561 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     7621 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     7681 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     7741 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     7801 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     7861 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     7921 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     7981 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     8041 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     8101 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     8161 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     8221 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     8281 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     8341 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     8401 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     8461 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     8521 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     8581 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     8641 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     8701 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     8761 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     8821 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     8881 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     8941 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     9001 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     9061 aaccgcctct ccccgcgcgt tggccgattc attaatgcag caagctcatg gctgactaat
     9121 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     9181 aggaggcttt tttggaggcc taggcttttg caaaaagctc cccgtggcac gacaggtttc
     9241 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     9301 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     9361 aacaatttca cacaggaaac agctatgaca tgattacgaa tttcacaaat aaagcatttt
     9421 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     9481 tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta
     9541 ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt
     9601 cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagt
//
