LOCUS       Exported                9059 bp ds-DNA     circular SYN 25-NOV-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9059)
  AUTHORS   Triple Threat
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Mar 13, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9059
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     492..511
                     /label=PCDNA3.0-F2
     primer_bind     494..514
                     /label=PCDNA3.1-F
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     complement(2076..2095)
                     /label=PLKO1-R
     primer_bind     2088..2111
                     /label=U6-pll3-7
     primer_bind     2189..2241
                     /label=CAG-F
     promoter        2217..3935
                     /label=CAG promoter
                     /label=CAG
                     /note="vntifkey=29"
     primer_bind     complement(2381..2401)
                     /label=CAGR2
     primer_bind     3216..3226
                     /label=PLB-F
     primer_bind     3216..3226
                     /label=PJET1-2-F
     primer_bind     3812..3829
                     /label=PPY-F
     primer_bind     3852..3867
                     /label=PMlink-F
     primer_bind     3895..3914
                     /label=pCAG-F
     misc_feature    3946..3966
                     /label=DR+1
     misc_feature    3967..3986
                     /label=sg-MYC-1
     misc_feature    3987..4007
                     /label=DR+1
     misc_feature    4008..4027
                     /label=sg-MYC-2
     misc_feature    4028..4048
                     /label=DR+1
     misc_feature    4049..4068
                     /label=sg-MYC-3
     misc_feature    4069..4089
                     /label=DR+1
     misc_feature    4090..4109
                     /label=sg-MYC-4
     misc_feature    4110..4130
                     /label=DR+1
     misc_feature    4131..4150
                     /label=sg-MYC-5
     misc_feature    4151..4171
                     /label=DR+1
     misc_feature    4172..4191
                     /label=sg-MYC-6
     misc_feature    4192..4212
                     /label=DR+1
     misc_feature    4213..4232
                     /label=sg-MYC-7
     misc_feature    4233..4253
                     /label=DR+1
     misc_feature    4254..4273
                     /label=sg-MYC-8
     misc_feature    4274..4294
                     /label=DR+1
     misc_feature    4295..4314
                     /label=sg-MYC-9
     misc_feature    4315..4335
                     /label=DR+1
     misc_feature    4336..4355
                     /label=sg-MYC-10
     misc_feature    4356..4376
                     /label=DR+1
     misc_feature    4377..4396
                     /label=sg-MYC-11
     misc_feature    4397..4417
                     /label=DR+1
     misc_feature    4418..4437
                     /label=sg-MYC-12
     misc_feature    4438..4458
                     /label=DR+1
     misc_feature    4459..4478
                     /label=sg-MYC-13
     misc_feature    4479..4499
                     /label=DR+1
     misc_feature    4500..4519
                     /label=sg-MYC-14
     misc_feature    4520..4540
                     /label=DR+1
     misc_feature    4541..4560
                     /label=sg-MYC-15
     misc_feature    4561..4581
                     /label=DR+1
     misc_feature    4582..4601
     misc_feature    4582..4601
     misc_feature    4582..4601
                     /label=16
     primer_bind     4582..4601
                     /label=20-1
     misc_feature    4582..4600
     misc_feature    4582..4599
     misc_feature    complement(4582..4592)
     misc_feature    complement(4587..4601)
     misc_feature    complement(4598..4601)
     misc_feature    complement(4599..4601)
     misc_feature    complement(4600..4601)
     misc_feature    4602..4622
                     /label=DR+1
     misc_feature    4623..4642
     misc_feature    complement(4623..4642)
     misc_feature    4623..4642
                     /label=17
     misc_feature    4623..4624
     misc_feature    4633..4642
     misc_feature    4643..4663
                     /label=DR+1
     misc_feature    4664..4683
     misc_feature    4664..4683
     misc_feature    4664..4683
                     /label=18
     misc_feature    4664..4682
     misc_feature    complement(4664..4677)
     misc_feature    complement(4664..4676)
     misc_feature    complement(4664..4665)
     misc_feature    complement(4676..4683)
     misc_feature    4684..4704
                     /label=DR+1
     misc_feature    4705..4724
     misc_feature    4705..4724
     misc_feature    4705..4724
                     /label=19
     misc_feature    4705..4723
     misc_feature    4705..4722
     misc_feature    4705..4721
     misc_feature    4705..4720
     misc_feature    4705..4719
     misc_feature    4705..4718
     misc_feature    4705..4717
     misc_feature    4705..4716
     misc_feature    4705..4715
     misc_feature    4705..4714
     misc_feature    4725..4745
                     /label=DR+1
     misc_feature    4746..4765
     misc_feature    4746..4765
     misc_feature    4746..4765
                     /label=20
     misc_feature    4766..4786
                     /label=DR+1
     misc_feature    4787..4806
     misc_feature    4787..4806
     misc_feature    4787..4806
                     /label=21
     misc_feature    4787..4805
     misc_feature    4787..4804
     misc_feature    4787..4803
     misc_feature    4787..4802
     misc_feature    4787..4801
     misc_feature    4787..4800
     misc_feature    4787..4790
     misc_feature    4807..4827
                     /label=DR+1
     misc_feature    4828..4847
     misc_feature    complement(4828..4847)
     misc_feature    4828..4847
                     /label=22
     misc_feature    4828..4830
     misc_feature    4828..4829
     misc_feature    4828
     misc_feature    4848..4868
                     /label=DR+1
     misc_feature    4869..4888
     misc_feature    4869..4888
     misc_feature    4869..4888
                     /label=23
     misc_feature    4869..4887
     misc_feature    4869..4886
     misc_feature    4869..4879
     misc_feature    4869..4878
     misc_feature    4869..4877
     misc_feature    4881..4888
     misc_feature    complement(4888)
     misc_feature    4889..4909
                     /label=DR+1
     misc_feature    4910..4929
     misc_feature    complement(4910..4929)
     misc_feature    4910..4929
                     /label=24
     misc_feature    4910..4919
     misc_feature    4910..4918
     misc_feature    4910..4917
     misc_feature    4911..4929
     misc_feature    4930..4950
                     /label=DR+1
     misc_feature    4951..4970
     misc_feature    complement(4951..4970)
     misc_feature    4951..4970
     misc_feature    4951..4970
     misc_feature    4951..4970
                     /label=25
     misc_feature    4951..4966
     misc_feature    4951..4965
     misc_feature    4951
     misc_feature    complement(4952..4970)
     misc_feature    complement(4963..4970)
     misc_feature    complement(4964..4970)
     misc_feature    4971..4991
                     /label=DR+1
     misc_feature    4992..5011
     misc_feature    4992..5011
     misc_feature    4992..5011
                     /label=26
     misc_feature    complement(4993..5011)
     misc_feature    5012..5032
                     /label=DR+1
     misc_feature    5033..5052
     misc_feature    complement(5033..5052)
     misc_feature    5033..5052
                     /label=27
     misc_feature    5033..5050
     misc_feature    5033..5049
     misc_feature    5033..5034
     misc_feature    5033
     misc_feature    complement(5034..5052)
     misc_feature    5053..5073
                     /label=DR+1
     misc_feature    5074..5093
     misc_feature    5074..5093
     misc_feature    5074..5093
                     /label=28
     misc_feature    5094..5114
                     /label=DR+1
     misc_feature    5115..5134
     misc_feature    complement(5115..5134)
     misc_feature    5115..5134
                     /label=29
     misc_feature    5115..5130
     misc_feature    complement(5116..5134)
     misc_feature    complement(5117..5134)
     misc_feature    5135..5155
                     /label=DR+1
     misc_feature    5156..5175
     misc_feature    5156..5175
     misc_feature    5156..5175
                     /label=30
     misc_feature    5156..5174
     misc_feature    5156..5170
     misc_feature    5156..5166
     misc_feature    5176..5196
                     /label=DR+1
     misc_feature    5197..5216
     misc_feature    complement(5197..5216)
     misc_feature    5197..5216
                     /label=31
     misc_feature    5197..5205
     misc_feature    complement(5198..5216)
     misc_feature    5217..5237
                     /label=DR+1
     misc_feature    5238..5257
     misc_feature    5238..5257
     misc_feature    5238..5257
                     /label=32
     misc_feature    5238..5256
     misc_feature    5238..5255
     misc_feature    5238..5249
     misc_feature    5238..5248
     misc_feature    5238..5247
     misc_feature    5250..5257
     misc_feature    5258..5278
                     /label=DR+1
     misc_feature    5279..5298
     misc_feature    complement(5279..5298)
     misc_feature    5279..5298
                     /label=33
     misc_feature    complement(5280..5298)
     misc_feature    complement(5281..5298)
     misc_feature    complement(5282..5298)
     misc_feature    complement(5283..5298)
     misc_feature    complement(5284..5298)
     misc_feature    complement(5285..5298)
     misc_feature    complement(5286..5298)
     misc_feature    complement(5291..5298)
     misc_feature    5299..5319
                     /label=DR+1
     misc_feature    5320..5339
     misc_feature    complement(5320..5339)
     misc_feature    5320..5339
                     /label=34
     misc_feature    complement(5335..5339)
     misc_feature    5340..5360
                     /label=DR+1
     misc_feature    5361..5380
     misc_feature    5361..5380
     misc_feature    complement(5361..5380)
     misc_feature    5361..5380
                     /label=35
     misc_feature    5361..5379
     misc_feature    5361..5378
     misc_feature    5361..5374
     misc_feature    5361..5373
     misc_feature    5361..5372
     misc_feature    5361..5371
     misc_feature    complement(5362..5380)
     misc_feature    complement(5363..5380)
     misc_feature    complement(5364..5380)
     misc_feature    complement(5365..5380)
     misc_feature    5381..5401
                     /label=DR+1
     misc_feature    5402..5421
     misc_feature    complement(5402..5421)
     misc_feature    5402..5421
                     /label=36
     misc_feature    complement(5403..5421)
     misc_feature    complement(5404..5421)
     misc_feature    complement(5419..5421)
     misc_feature    complement(5420..5421)
     misc_feature    complement(5421)
     misc_feature    5449..6037
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5484..5506)
                     /label=PCDNA3-4-R
     primer_bind     complement(5502..5522)
                     /label=WPRE-R
     primer_bind     complement(5525..5542)
                     /label=GIPZ sequencing primer
     CDS             complement(5920..5931)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6112..6345
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     6203..6222
                     /label=PCDNA3.0-F2
     primer_bind     6205..6225
                     /label=PCDNA3.1-F
     primer_bind     complement(6464..6482)
                     /label=pBRforEco
     primer_bind     complement(6516..6535)
                     /label=PBR322F
     primer_bind     complement(6532..6551)
                     /label=PTRG-R
     promoter        6550..6654
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6610..6628)
                     /label=BLA-Prorev
     CDS             6655..7515
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6961..6983
                     /label=AmpR-1F
     primer_bind     complement(7186..7204)
                     /label=AmpR-1R
     primer_bind     7408..7431
                     /label=PrimerBlaM1
     primer_bind     7408..7431
                     /label=Primer Bla M1
     primer_bind     complement(7613..7636)
                     /label=PrimerBlaRV
     primer_bind     complement(7613..7636)
                     /label=Primer Bla RV
     rep_origin      7686..8274
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8461..8473
                     /label=PCAMBIA2300-5
     primer_bind     8537..8556
                     /label=SV40pro-F
     primer_bind     complement(8560..8571)
                     /label=SV40pro-F
     primer_bind     8592..8609
                     /label=SV40ori-3for
     protein_bind    8685..8706
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     8715..8734
                     /label=PCAMBIA1301-F(即为P1)
     primer_bind     8716..8737
                     /label=SRprimer
     primer_bind     8718..8737
                     /label=ptarget-R
     promoter        8721..8751
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     8735..8754
                     /label=Y-M13R-48
     primer_bind     8742..8759
                     /label=PCAMBIA1300-5
     protein_bind    8759..8775
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8762..8781
                     /label=pQE30-F
     primer_bind     8764..8787
                     /label=M13R-48
     primer_bind     8764..8785
                     /label=PQ60-F
     primer_bind     8783..8799
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8783..8799
                     /label=pDONR222-R
     primer_bind     8783..8799
                     /label=M13Rev(-26)
     primer_bind     8790..8808
                     /label=M13mp18-R
     primer_bind     8834..8853
                     /label=PIRES-R
     primer_bind     8839..8858
                     /label=PCI-NEO-R
     primer_bind     8849..8868
                     /label=EBVrev
     primer_bind     8857..8876
                     /label=pCMV-R
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggaca
     2221 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     2281 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     2341 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     2401 ccattgacgt caatgggtgg actatttacg gtaaactgcc cacttggcag tacatcaagt
     2461 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     2521 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     2581 catcgctatt accatgggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc
     2641 ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg
     2701 gggcgggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg
     2761 gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
     2821 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc
     2881 gctgcgttgc cttcgccccg tgccccgctc cgcgccgcct cgcgccgccc gccccggctc
     2941 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt
     3001 aattagcgct tggtttaatg acggctcgtt tcttttctgt ggctgcgtga aagccttaaa
     3061 gggctccggg agggcccttt gtgcgggggg gagcggctcg gggggtgcgt gcgtgtgtgt
     3121 gtgcgtgggg agcgccgcgt gcggcccgcg ctgcccggcg gctgtgagcg ctgcgggcgc
     3181 ggcgcggggc tttgtgcgct ccgcgtgtgc gcgaggggag cgcggccggg ggcggtgccc
     3241 cgcggtgcgg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg
     3301 gtgagcaggg ggtgtgggcg cggcggtcgg gctgtaaccc ccccctgcac ccccctcccc
     3361 gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt ggcgcggggc
     3421 tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg gggccgcctc
     3481 gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag
     3541 gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc
     3601 ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg
     3661 ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg
     3721 cgtcgccgcg ccgccgtccc cttctccatc tccagcctcg gggctgtccg cagggggacg
     3781 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct
     3841 ctagtgcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg
     3901 tgctggttat tgtgctgtct catcattttg gcaaagggcg gccgctaatt tctactaagt
     3961 gtagatcccc gaccccaaca ccttcttaat ttctactaag tgtagatgct acacaagaat
     4021 ctagaattaa tttctactaa gtgtagatgc cggagaccct ggattctata atttctacta
     4081 agtgtagata ctagaggacc aactcagtat aatttctact aagtgtagat gcaggggctg
     4141 ataacattct taatttctac taagtgtaga tgcaggaaat gtaaacgaag ataatttcta
     4201 ctaagtgtag atagtagcag ccttgtctag cctaatttct actaagtgta gatcatgaga
     4261 gaaaacaaag ccataatttc tactaagtgt agatacatag gaaggcacaa ataataattt
     4321 ctactaagtg tagatgctgc cttccaggca ttaattaatt tctactaagt gtagatactt
     4381 aaaacagctc aatacataat ttctactaag tgtagatcaa tgcccagatc cactcactaa
     4441 tttctactaa gtgtagatat ggaactcact atgaaacata atttctacta agtgtagatc
     4501 ctggcacgtg tccctggtct aatttctact aagtgtagat gcttcaatta gagtaatgcc
     4561 taatttctac taagtgtaga taacccagca aatgtctatt ctaatttcta ctaagtgtag
     4621 atagataatc tctttgtgat tctaatttct actaagtgta gataatgaag aggaaaatta
     4681 atctaatttc tactaagtgt agatgagatg gcacgtcacc acattaattt ctactaagtg
     4741 tagatccagt ctccaccact ccctataatt tctactaagt gtagatgtct atgtacttgt
     4801 gaattataat ttctactaag tgtagattgt gttgctcagg ctggtcttaa tttctactaa
     4861 gtgtagatgt agagacaggg tttcaccata atttctacta agtgtagatc tttaatgaat
     4921 gcagtatact aatttctact aagtgtagat ttggaggtgg tggagggaga taatttctac
     4981 taagtgtaga ttctgaagtg cagagttggt ataatttcta ctaagtgtag atattctgta
     5041 agatgatcat ggtaatttct actaagtgta gatctctagc tacacactat tcttaatttc
     5101 tactaagtgt agatcatcgt tggcctctac ccactaattt ctactaagtg tagatagaca
     5161 ggatctcact ctgtctaatt tctactaagt gtagatttgc atactcattt tagtattaat
     5221 ttctactaag tgtagatgtg gagacggggt ttcaccataa tttctactaa gtgtagatta
     5281 cagggtgaga ccctgtctta atttctacta agtgtagatg tctggggaac tgcctgaact
     5341 aatttctact aagtgtagat atgccgtgga ataacacaaa taatttctac taagtgtaga
     5401 tcagtgagac ctcatctcta ctttttttct cgagaatcga tagatcctaa tcaacctctg
     5461 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     5521 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     5581 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     5641 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     5701 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     5761 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     5821 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     5881 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     5941 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     6001 cagacgagtc ggatctccct ttgggccgcc tccccgcctg agatccttta agaccaatga
     6061 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     6121 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     6181 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     6241 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     6301 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     6361 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcccg
     6421 ggttaattaa ggaaagggct agatcattct tgaagacgaa agggcctcgt gatacgccta
     6481 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     6541 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     6601 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     6661 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     6721 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     6781 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     6841 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     6901 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     6961 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     7021 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     7081 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     7141 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     7201 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     7261 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     7321 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     7381 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     7441 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     7501 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     7561 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     7621 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     7681 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     7741 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     7801 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     7861 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     7921 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     7981 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     8041 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     8101 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     8161 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     8221 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     8281 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     8341 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     8401 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     8461 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag caagctcatg
     8521 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     8581 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc cccgtggcac
     8641 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     8701 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     8761 gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa tttcacaaat
     8821 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     8881 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
     8941 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
     9001 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagt
//
