LOCUS       Exported                9608 bp ds-DNA     circular SYN 25-NOV-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9608)
  AUTHORS   Triple Threat
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Mar 13, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9608
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     492..511
                     /label=PCDNA3.0-F2
     primer_bind     494..514
                     /label=PCDNA3.1-F
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     complement(2076..2095)
                     /label=PLKO1-R
     primer_bind     2088..2111
                     /label=U6-pll3-7
     primer_bind     2189..2241
                     /label=CAG-F
     promoter        2217..3935
                     /label=CAG promoter
                     /label=CAG
                     /note="vntifkey=29"
     primer_bind     complement(2381..2401)
                     /label=CAGR2
     primer_bind     3216..3226
                     /label=PJET1-2-F
     primer_bind     3216..3226
                     /label=PLB-F
     primer_bind     3812..3829
                     /label=PPY-F
     primer_bind     3852..3867
                     /label=PMlink-F
     primer_bind     3895..3914
                     /label=pCAG-F
     misc_feature    3971..3991
                     /label=DR+1
     misc_feature    4012..4032
                     /label=DR+1
     misc_feature    4053..4073
                     /label=DR+1
     misc_feature    4094..4114
                     /label=DR+1
     misc_feature    4135..4155
                     /label=DR+1
     misc_feature    4176..4196
                     /label=DR+1
     misc_feature    4217..4237
                     /label=DR+1
     misc_feature    4258..4278
                     /label=DR+1
     misc_feature    4299..4319
                     /label=DR+1
     misc_feature    4340..4360
                     /label=DR+1
     misc_feature    4381..4401
                     /label=DR+1
     misc_feature    4422..4442
                     /label=DR+1
     misc_feature    4463..4483
                     /label=DR+1
     misc_feature    4504..4524
                     /label=DR+1
     misc_feature    4545..4565
                     /label=DR+1
     misc_feature    4586..4606
                     /label=DR+1
     misc_feature    4627..4647
                     /label=DR+1
     misc_feature    4668..4688
                     /label=DR+1
     primer_bind     4689..4708
                     /label=21-1
     misc_feature    4709..4729
                     /label=DR+1
     misc_feature    4750..4770
                     /label=DR+1
     misc_feature    4791..4811
                     /label=DR+1
     misc_feature    4832..4852
                     /label=DR+1
     misc_feature    4873..4893
                     /label=DR+1
     misc_feature    4914..4934
                     /label=DR+1
     misc_feature    4955..4975
                     /label=DR+1
     misc_feature    4996..5016
                     /label=DR+1
     misc_feature    5037..5057
                     /label=DR+1
     misc_feature    5078..5098
                     /label=DR+1
     misc_feature    5119..5139
                     /label=DR+1
     misc_feature    5160..5180
                     /label=DR+1
     misc_feature    5201..5221
                     /label=DR+1
     misc_feature    5242..5262
                     /label=DR+1
     misc_feature    5283..5303
                     /label=DR+1
     misc_feature    5324..5344
                     /label=DR+1
     misc_feature    5365..5385
                     /label=DR+1
     misc_feature    5406..5426
                     /label=DR+1
     misc_feature    5447..5467
                     /label=DR+1
     misc_feature    5488..5508
                     /label=DR+1
     misc_feature    5529..5549
                     /label=DR+1
     misc_feature    5570..5590
                     /label=DR+1
     misc_feature    5611..5631
                     /label=DR+1
     misc_feature    5652..5672
                     /label=DR+1
     misc_feature    5693..5713
                     /label=DR+1
     misc_feature    5734..5754
                     /label=DR+1
     misc_feature    5775..5795
                     /label=DR+1
     misc_feature    5816..5836
                     /label=DR+1
     misc_feature    5857..5877
                     /label=DR+1
     misc_feature    5898..5918
                     /label=DR+1
     misc_feature    5939..5959
                     /label=DR+1
     misc_feature    5998..6586
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6033..6055)
                     /label=PCDNA3-4-R
     primer_bind     complement(6051..6071)
                     /label=WPRE-R
     primer_bind     complement(6074..6091)
                     /label=GIPZ sequencing primer
     primer_bind     complement(6339..6348)
                     /label=24XCCAT01-R
     CDS             complement(6469..6480)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6661..6894
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     6752..6771
                     /label=PCDNA3.0-F2
     primer_bind     6754..6774
                     /label=PCDNA3.1-F
     primer_bind     complement(7013..7031)
                     /label=pBRforEco
     primer_bind     complement(7065..7084)
                     /label=PBR322F
     primer_bind     complement(7081..7100)
                     /label=PTRG-R
     promoter        7099..7203
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7159..7177)
                     /label=BLA-Prorev
     CDS             7204..8064
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7510..7532
                     /label=AmpR-1F
     primer_bind     complement(7735..7753)
                     /label=AmpR-1R
     primer_bind     7957..7980
                     /label=Primer Bla M1
     primer_bind     7957..7980
                     /label=PrimerBlaM1
     primer_bind     complement(8162..8185)
                     /label=Primer Bla RV
     primer_bind     complement(8162..8185)
                     /label=PrimerBlaRV
     rep_origin      8235..8823
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9010..9022
                     /label=PCAMBIA2300-5
     primer_bind     9086..9105
                     /label=SV40pro-F
     primer_bind     complement(9109..9120)
                     /label=SV40pro-F
     primer_bind     9141..9158
                     /label=SV40ori-3for
     protein_bind    9234..9255
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     9264..9283
                     /label=PCAMBIA1301-F(即为P1)
     primer_bind     9265..9286
                     /label=SRprimer
     primer_bind     9267..9286
                     /label=ptarget-R
     promoter        9270..9300
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     9284..9303
                     /label=Y-M13R-48
     primer_bind     9291..9308
                     /label=PCAMBIA1300-5
     protein_bind    9308..9324
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9311..9330
                     /label=pQE30-F
     primer_bind     9313..9336
                     /label=M13R-48
     primer_bind     9313..9334
                     /label=PQ60-F
     primer_bind     9332..9348
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9332..9348
                     /label=M13Rev(-26)
     primer_bind     9332..9348
                     /label=pDONR222-R
     primer_bind     9339..9357
                     /label=M13mp18-R
     primer_bind     9383..9402
                     /label=PIRES-R
     primer_bind     9388..9407
                     /label=PCI-NEO-R
     primer_bind     9398..9417
                     /label=EBVrev
     primer_bind     9406..9425
                     /label=pCMV-R
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggaca
     2221 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     2281 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     2341 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     2401 ccattgacgt caatgggtgg actatttacg gtaaactgcc cacttggcag tacatcaagt
     2461 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     2521 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     2581 catcgctatt accatgggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc
     2641 ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg
     2701 gggcgggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg
     2761 gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
     2821 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc
     2881 gctgcgttgc cttcgccccg tgccccgctc cgcgccgcct cgcgccgccc gccccggctc
     2941 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt
     3001 aattagcgct tggtttaatg acggctcgtt tcttttctgt ggctgcgtga aagccttaaa
     3061 gggctccggg agggcccttt gtgcgggggg gagcggctcg gggggtgcgt gcgtgtgtgt
     3121 gtgcgtgggg agcgccgcgt gcggcccgcg ctgcccggcg gctgtgagcg ctgcgggcgc
     3181 ggcgcggggc tttgtgcgct ccgcgtgtgc gcgaggggag cgcggccggg ggcggtgccc
     3241 cgcggtgcgg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg
     3301 gtgagcaggg ggtgtgggcg cggcggtcgg gctgtaaccc ccccctgcac ccccctcccc
     3361 gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt ggcgcggggc
     3421 tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg gggccgcctc
     3481 gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag
     3541 gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc
     3601 ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg
     3661 ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg
     3721 cgtcgccgcg ccgccgtccc cttctccatc tccagcctcg gggctgtccg cagggggacg
     3781 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct
     3841 ctagtgcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg
     3901 tgctggttat tgtgctgtct catcattttg gcaaagggcg gccgcatctt gtggaaagga
     3961 cgaaacaccg taatttctac taagtgtaga tggtgaggcc aggctcatgg ataatttcta
     4021 ctaagtgtag atagtgtggc taatagtgtt aataatttct actaagtgta gatagatgac
     4081 aggacagaag gaataatttc tactaagtgt agatgttctc cctccctgag cacctaattt
     4141 ctactaagtg tagatcgtac ccctactcat ctgattaatt tctactaagt gtagatgagg
     4201 tccttaccct gctgcataat ttctactaag tgtagatctg ccagagatgc aactgtctaa
     4261 tttctactaa gtgtagatga cagaacactc cctgaaggta atttctacta agtgtagatg
     4321 gaggttctaa gaaatacaat aatttctact aagtgtagat ctaggggatg cttaccaact
     4381 taatttctac taagtgtaga tgtgctatat tacagtctcc ataatttcta ctaagtgtag
     4441 atgtgacatt agacagatga gctaatttct actaagtgta gatggtgata ttagccaaag
     4501 ctttaatttc tactaagtgt agatttacag cccacccaga aaattaattt ctactaagtg
     4561 tagattgagg aggactcctg cttgttaatt tctactaagt gtagatcata gagaaaacta
     4621 tgcagataat ttctactaag tgtagatctg acaccctgcc gtttatttaa tttctactaa
     4681 gtgtagatgg aatccacatc aaaagcaata atttctacta agtgtagatg taacatagga
     4741 aagagtaagt aatttctact aagtgtagat actgcaccgg gccaaattaa taatttctac
     4801 taagtgtaga tattgtgact cagggttaaa ttaatttcta ctaagtgtag atcgtgcaac
     4861 cctggtgctg actaatttct actaagtgta gatgccgacg gactaagggt tactaatttc
     4921 tactaagtgt agatagtaga gaaaccacgt cacataattt ctactaagtg tagatggtat
     4981 aatgaactag gggtataatt tctactaagt gtagatactt aggcagagct ggactgtaat
     5041 ttctactaag tgtagatgca gctagcagtg ctgcagataa tttctactaa gtgtagatac
     5101 ctcctggcta ctccctccta atttctacta agtgtagata gatggcagtg ggcttacagt
     5161 aatttctact aagtgtagat ctggcaacag ccaatattag taatttctac taagtgtaga
     5221 taaggaccac agcatactgc ctaatttcta ctaagtgtag atcccagaag cttgagtttc
     5281 agtaatttct actaagtgta gatgtccagc tttctcagca ctttaatttc tactaagtgt
     5341 agatgttacc ttaggaggaa ttcataattt ctactaagtg tagatgttgg tgagaggtga
     5401 gagagtaatt tctactaagt gtagataaag aggacacttc tgcagataat ttctactaag
     5461 tgtagatcgt tataaacttg gaaattctaa tttctactaa gtgtagatgg tgtggcccgg
     5521 agcactgata atttctacta agtgtagatg ttgtcctgcg agtagcagat aatttctact
     5581 aagtgtagat gtaaagggat agaaagtgct taatttctac taagtgtaga tcaagaggaa
     5641 gtgagtcaca ctaatttcta ctaagtgtag atatcctgca atcatcatag aataatttct
     5701 actaagtgta gatatctgta caaagattcc atttaatttc tactaagtgt agatagatga
     5761 ctcatgccag attctaattt ctactaagtg tagatacagt agctatgttg gctgataatt
     5821 tctactaagt gtagataaca tagtgtagcc ttaagttaat ttctactaag tgtagatgct
     5881 taattggaag gaatcattaa tttctactaa gtgtagatga agggatgatt aaagagaata
     5941 atttctacta agtgtagatt gcccccaccc agttccgtcc gagaatcgat agatcctaat
     6001 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     6061 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     6121 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     6181 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     6241 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     6301 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     6361 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     6421 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     6481 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     6541 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctga gatcctttaa
     6601 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     6661 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     6721 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     6781 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     6841 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     6901 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     6961 agaggcccgg gttaattaag gaaagggcta gatcattctt gaagacgaaa gggcctcgtg
     7021 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     7081 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     7141 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     7201 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     7261 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     7321 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     7381 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     7441 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     7501 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     7561 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     7621 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     7681 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     7741 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     7801 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     7861 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     7921 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     7981 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     8041 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     8101 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     8161 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     8221 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     8281 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     8341 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     8401 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     8461 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     8521 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     8581 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     8641 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     8701 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     8761 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     8821 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     8881 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     8941 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     9001 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     9061 aagctcatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     9121 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     9181 ccgtggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     9241 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     9301 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat
     9361 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     9421 gtatcttatc atgtctggat caactggata actcaagcta accaaaatca tcccaaactt
     9481 cccaccccat accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt
     9541 gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac
     9601 ttagtagt
//
