LOCUS       Exported                9572 bp ds-DNA     circular SYN 25-NOV-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9572)
  AUTHORS   Triple Threat
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Mar 13, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9572
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     492..511
                     /label=PCDNA3.0-F2
     primer_bind     494..514
                     /label=PCDNA3.1-F
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     complement(2076..2095)
                     /label=PLKO1-R
     primer_bind     2088..2111
                     /label=U6-pll3-7
     primer_bind     2189..2241
                     /label=CAG-F
     promoter        2217..3935
                     /label=CAG promoter
                     /label=CAG
                     /note="vntifkey=29"
     primer_bind     complement(2381..2401)
                     /label=CAGR2
     primer_bind     3216..3226
                     /label=PJET1-2-F
     primer_bind     3216..3226
                     /label=PLB-F
     primer_bind     3812..3829
                     /label=PPY-F
     primer_bind     3852..3867
                     /label=PMlink-F
     primer_bind     3895..3914
                     /label=pCAG-F
     misc_feature    3971..3990
                     /label=LbDR
     misc_feature    3991..4010
                     /label=H3C1-sg1
     misc_feature    4011..4030
                     /label=LbDR
     misc_feature    4031..4050
                     /label=H3C1-sg2
     misc_feature    4051..4070
                     /label=LbDR
     misc_feature    4071..4090
                     /label=H3C1-sg3
     misc_feature    4091..4110
                     /label=LbDR
     misc_feature    4111..4130
                     /label=H3C1-sg4
     misc_feature    4131..4150
                     /label=LbDR
     misc_feature    4151..4170
                     /label=H3C1-sg5
     misc_feature    4171..4190
                     /label=LbDR
     misc_feature    4191..4210
                     /label=H3C1-sg5
     misc_feature    4211..4230
                     /label=LbDR
     misc_feature    4231..4250
                     /label=H3C1-sg6
     misc_feature    4251..4270
                     /label=LbDR
     misc_feature    4271..4290
                     /label=H3C1-sg7
     misc_feature    4291..4310
                     /label=LbDR
     misc_feature    4311..4330
                     /label=8
     misc_feature    4331..4350
                     /label=LbDR
     misc_feature    4351..4370
                     /label=H3C1-sg9
     misc_feature    4371..4390
                     /label=LbDR
     misc_feature    4391..4410
                     /label=H3C1-sg10
     misc_feature    4411..4430
                     /label=LbDR
     misc_feature    4431..4450
                     /label=H3C1-sg11
     misc_feature    4451..4470
                     /label=LbDR
     misc_feature    4471..4490
                     /label=H3C1-sg12
     misc_feature    4491..4510
                     /label=LbDR
     misc_feature    4511..4530
                     /label=H3C1-sg13
     misc_feature    4531..4550
                     /label=LbDR
     misc_feature    4551..4570
                     /label=H3C1-sg14
     misc_feature    4571..4590
                     /label=LbDR
     misc_feature    4591..4610
                     /label=H3C1-sg15
     misc_feature    4611..4630
                     /label=LbDR
     misc_feature    4631..4650
                     /label=H3C1-sg16
     misc_feature    4651..4670
                     /label=LbDR
     misc_feature    4671..4690
                     /label=H3C1-sg17
     misc_feature    4691..4710
                     /label=LbDR
     misc_feature    4711..4730
                     /label=H3C1-sg18
     misc_feature    4731..4750
                     /label=LbDR
     misc_feature    4751..4770
                     /label=H3C1-sg19
     primer_bind     4751..4770
                     /label=24-1
     misc_feature    4771..4790
                     /label=LbDR
     misc_feature    4791..4810
                     /label=20
     misc_feature    4811..4830
                     /label=LbDR
     misc_feature    4831..4850
                     /label=H3C1-sg21
     misc_feature    4851..4870
                     /label=LbDR
     misc_feature    4871..4890
                     /label=H3C1-sg22
     misc_feature    4891..4910
                     /label=LbDR
     misc_feature    4911..4930
                     /label=H3C1-sg23
     misc_feature    4931..4950
                     /label=LbDR
     misc_feature    4951..4970
                     /label=H3C1-sg24
     misc_feature    4971..4990
                     /label=LbDR
     misc_feature    4991..5010
                     /label=H3C1-sg25
     misc_feature    5011..5030
                     /label=LbDR
     misc_feature    5031..5050
                     /label=H3C1-sg26
     misc_feature    5051..5070
                     /label=LbDR
     misc_feature    5071..5090
                     /label=H3C1-sg27
     misc_feature    5091..5110
                     /label=LbDR
     misc_feature    5111..5130
                     /label=H3C1-sg28
     misc_feature    5131..5150
                     /label=LbDR
     misc_feature    5151..5170
                     /label=H3C1-sg29
     misc_feature    5171..5190
                     /label=LbDR
     misc_feature    5191..5210
                     /label=H3C1-sg30
     misc_feature    5211..5230
                     /label=LbDR
     misc_feature    5231..5250
                     /label=H3C1-sg31
     misc_feature    5251..5270
                     /label=LbDR
     misc_feature    5271..5290
                     /label=H3C1-sg32
     misc_feature    5291..5310
                     /label=LbDR
     misc_feature    5311..5330
                     /label=H3C1-sg33
     misc_feature    5331..5350
                     /label=LbDR
     misc_feature    5351..5370
                     /label=H3C1-sg34
     misc_feature    5371..5390
                     /label=LbDR
     misc_feature    5391..5410
                     /label=H3C1-sg35
     misc_feature    5411..5430
                     /label=LbDR
     misc_feature    5431..5450
                     /label=H3C1-sg36
     misc_feature    5451..5470
                     /label=LbDR
     misc_feature    5471..5490
                     /label=H3C1-sg37
     misc_feature    5491..5510
                     /label=LbDR
     misc_feature    5511..5530
                     /label=H3C1-sg38
     misc_feature    5531..5550
                     /label=LbDR
     misc_feature    5551..5570
                     /label=H3C1-sg39
     misc_feature    5571..5590
                     /label=LbDR
     misc_feature    5591..5610
                     /label=H3C1-sg40
     misc_feature    5611..5630
                     /label=LbDR
     misc_feature    5631..5650
                     /label=41
     misc_feature    5651..5670
                     /label=LbDR
     misc_feature    5671..5690
                     /label=H3C1-sg42
     misc_feature    5691..5710
                     /label=LbDR
     misc_feature    5711..5730
                     /label=H3C1-sg43
     misc_feature    5731..5750
                     /label=LbDR
     misc_feature    5751..5770
                     /label=44
     misc_feature    5771..5790
                     /label=LbDR
     misc_feature    5791..5810
                     /label=H3C1-sg45
     misc_feature    5811..5830
                     /label=LbDR
     misc_feature    5831..5850
                     /label=H3C1-sg46
     misc_feature    5851..5870
                     /label=LbDR
     misc_feature    5871..5890
                     /label=48
     misc_feature    5891..5910
                     /label=LbDR
     misc_feature    5962..6550
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5997..6019)
                     /label=PCDNA3-4-R
     primer_bind     complement(6015..6035)
                     /label=WPRE-R
     primer_bind     complement(6038..6055)
                     /label=GIPZ sequencing primer
     primer_bind     complement(6303..6312)
                     /label=24XCCAT01-R
     CDS             complement(6433..6444)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6625..6858
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     6716..6735
                     /label=PCDNA3.0-F2
     primer_bind     6718..6738
                     /label=PCDNA3.1-F
     primer_bind     complement(6977..6995)
                     /label=pBRforEco
     primer_bind     complement(7029..7048)
                     /label=PBR322F
     primer_bind     complement(7045..7064)
                     /label=PTRG-R
     promoter        7063..7167
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7123..7141)
                     /label=BLA-Prorev
     CDS             7168..8028
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7474..7496
                     /label=AmpR-1F
     primer_bind     complement(7699..7717)
                     /label=AmpR-1R
     primer_bind     7921..7944
                     /label=Primer Bla M1
     primer_bind     7921..7944
                     /label=PrimerBlaM1
     primer_bind     complement(8126..8149)
                     /label=Primer Bla RV
     primer_bind     complement(8126..8149)
                     /label=PrimerBlaRV
     rep_origin      8199..8787
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8974..8986
                     /label=PCAMBIA2300-5
     primer_bind     9050..9069
                     /label=SV40pro-F
     primer_bind     complement(9073..9084)
                     /label=SV40pro-F
     primer_bind     9105..9122
                     /label=SV40ori-3for
     protein_bind    9198..9219
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     9228..9247
                     /label=PCAMBIA1301-F(即为P1)
     primer_bind     9229..9250
                     /label=SRprimer
     primer_bind     9231..9250
                     /label=ptarget-R
     promoter        9234..9264
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     9248..9267
                     /label=Y-M13R-48
     primer_bind     9255..9272
                     /label=PCAMBIA1300-5
     protein_bind    9272..9288
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9275..9294
                     /label=pQE30-F
     primer_bind     9277..9300
                     /label=M13R-48
     primer_bind     9277..9298
                     /label=PQ60-F
     primer_bind     9296..9312
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9296..9312
                     /label=M13Rev(-26)
     primer_bind     9296..9312
                     /label=pDONR222-R
     primer_bind     9303..9321
                     /label=M13mp18-R
     primer_bind     9347..9366
                     /label=PIRES-R
     primer_bind     9352..9371
                     /label=PCI-NEO-R
     primer_bind     9362..9381
                     /label=EBVrev
     primer_bind     9370..9389
                     /label=pCMV-R
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggaca
     2221 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     2281 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     2341 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     2401 ccattgacgt caatgggtgg actatttacg gtaaactgcc cacttggcag tacatcaagt
     2461 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     2521 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     2581 catcgctatt accatgggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc
     2641 ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg
     2701 gggcgggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg
     2761 gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
     2821 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc
     2881 gctgcgttgc cttcgccccg tgccccgctc cgcgccgcct cgcgccgccc gccccggctc
     2941 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt
     3001 aattagcgct tggtttaatg acggctcgtt tcttttctgt ggctgcgtga aagccttaaa
     3061 gggctccggg agggcccttt gtgcgggggg gagcggctcg gggggtgcgt gcgtgtgtgt
     3121 gtgcgtgggg agcgccgcgt gcggcccgcg ctgcccggcg gctgtgagcg ctgcgggcgc
     3181 ggcgcggggc tttgtgcgct ccgcgtgtgc gcgaggggag cgcggccggg ggcggtgccc
     3241 cgcggtgcgg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg
     3301 gtgagcaggg ggtgtgggcg cggcggtcgg gctgtaaccc ccccctgcac ccccctcccc
     3361 gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt ggcgcggggc
     3421 tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg gggccgcctc
     3481 gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag
     3541 gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc
     3601 ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg
     3661 ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg
     3721 cgtcgccgcg ccgccgtccc cttctccatc tccagcctcg gggctgtccg cagggggacg
     3781 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct
     3841 ctagtgcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg
     3901 tgctggttat tgtgctgtct catcattttg gcaaagggcg gccgcatctt gtggaaagga
     3961 cgaaacaccg aatttctact aagtgtagat gatgcacccg ttgccttagt aatttctact
     4021 aagtgtagat catacaccta tctatataca aatttctact aagtgtagat aagagagcac
     4081 ccactgattc aatttctact aagtgtagat atctgtgtac tacagctctt aatttctact
     4141 aagtgtagat ggtgtctact gttgatgcat aatttctact aagtgtagat gattccagct
     4201 ttccaaagaa aatttctact aagtgtagat agagctgcca acgacacacc aatttctact
     4261 aagtgtagat gcattgccaa gagctaatgc aatttctact aagtgtagat cattgaccat
     4321 ggcttagaat aatttctact aagtgtagat aatatgagat gacatctcac aatttctact
     4381 aagtgtagat aataacactc ctaagtcaat aatttctact aagtgtagat actctgaggt
     4441 atacaaacag aatttctact aagtgtagat ggattcaaag agcataatca aatttctact
     4501 aagtgtagat aaataagtcc gaaccatgct aatttctact aagtgtagat ctactcaatc
     4561 tatcaaagat aatttctact aagtgtagat cggagaggag gtgagcagta aatttctact
     4621 aagtgtagat gagctgcaac ccaaaatccc aatttctact aagtgtagat cagttaccac
     4681 tgctgaacat aatttctact aagtgtagat cttagtgcag ttcaattcta aatttctact
     4741 aagtgtagat gaggtccgtt gccagagatg aatttctact aagtgtagat cattgcattg
     4801 gaatgacata aatttctact aagtgtagat gtgtactcaa attaagcaca aatttctact
     4861 aagtgtagat aattaataac cctaatcaaa aatttctact aagtgtagat atagaactag
     4921 tattcttcta aatttctact aagtgtagat aaggataaac ctaggtagct aatttctact
     4981 aagtgtagat actccagaac attaggtttg aatttctact aagtgtagat tgagtaatag
     5041 tgtaatccta aatttctact aagtgtagat agctaatata aacaataccg aatttctact
     5101 aagtgtagat taagcaagga ataagaataa aatttctact aagtgtagat gagcgatgtg
     5161 tctcgcgaat aatttctact aagtgtagat agagagaagc ggtaccctct aatttctact
     5221 aagtgtagat gaagggggag tgataaatca aatttctact aagtgtagat gctggcaaga
     5281 aggcaaagaa aatttctact aagtgtagat acatagcctc atctagtaag aatttctact
     5341 aagtgtagat gagaagcata acatcatgag aatttctact aagtgtagat cgtgtgtgca
     5401 tatgagttaa aatttctact aagtgtagat aactgagaag cataacatat aatttctact
     5461 aagtgtagat ctaaagagct gaaggttata aatttctact aagtgtagat gtgctgaaca
     5521 gaataccaga aatttctact aagtgtagat agatacctcg cttattacac aatttctact
     5581 aagtgtagat agacatggtg actaacacag aatttctact aagtgtagat gcctaaattg
     5641 tgttccccca aatttctact aagtgtagat ctacaggctt cagtggaagc aatttctact
     5701 aagtgtagat aatgcatgcc ttctcaataa aatttctact aagtgtagat gggcatgttg
     5761 tatggatgaa aatttctact aagtgtagat ccatggtact ccttgtcccc aatttctact
     5821 aagtgtagat atttcatcgc atgtaaacct aatttctact aagtgtagat aggaaaagca
     5881 tactgtccat aatttctact aagtgtagat aagtggaaac tcctgaatca gaattctcga
     5941 cctcgagaat cgatagatcc taatcaacct ctggattaca aaatttgtga aagattgact
     6001 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     6061 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     6121 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     6181 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     6241 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     6301 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     6361 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     6421 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     6481 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     6541 gcctccccgc ctgagatcct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     6601 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     6661 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     6721 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     6781 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     6841 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     6901 caaagaaatg aatatcagag agtgagaggc ccgggttaat taaggaaagg gctagatcat
     6961 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     7021 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     7081 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     7141 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     7201 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     7261 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     7321 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     7381 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     7441 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     7501 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     7561 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     7621 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     7681 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     7741 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     7801 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     7861 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     7921 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     7981 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     8041 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     8101 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     8161 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     8221 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     8281 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     8341 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     8401 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     8461 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     8521 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     8581 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     8641 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     8701 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     8761 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     8821 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     8881 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     8941 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     9001 cgttggccga ttcattaatg cagcaagctc atggctgact aatttttttt atttatgcag
     9061 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     9121 gcctaggctt ttgcaaaaag ctccccgtgg cacgacaggt ttcccgactg gaaagcgggc
     9181 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     9241 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     9301 aacagctatg acatgattac gaatttcaca aataaagcat ttttttcact gcattctagt
     9361 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa
     9421 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg
     9481 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt
     9541 atttgttaaa tatgtactac aaacttagta gt
//
