LOCUS       Exported               12829 bp ds-DNA     circular SYN 19-NOV-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12829)
  AUTHORS   Triple Threat
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Mar 13, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12829
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     492..511
                     /label=PCDNA3.0-F2
     primer_bind     494..514
                     /label=PCDNA3.1-F
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     complement(2076..2095)
                     /label=PLKO1-R
     primer_bind     2088..2111
                     /label=U6-pll3-7
     promoter        2224..3405
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     primer_bind     complement(2296..2318)
                     /label=pCDH1-R
     primer_bind     complement(2341..2360)
                     /label=EF1A-R
     intron          2454..3396
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3353..3373
                     /label=EF-1aFwd(PEF-F)
     CDS             3418..3465
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             3472..7152
                     /codon_start=1
                     /label=dLbCas12a
                     /translation="SKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAED
                     YKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKEI
                     AKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFRNRENMFSE
                     EAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEGE
                     FFNFVLTQEGIRVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVLS
                     DRESLSFYGRGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKNG
                     PAISTISKRIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQL
                     QEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKDL
                     LDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYSK
                     DKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNGN
                     YEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKLI
                     DFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLVE
                     EGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKKE
                     ELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKIN
                     TEVRVLLKHDDNPYVIGIARGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTDY
                     HSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALADLNSGF
                     KNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQN
                     GFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALDY
                     KNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQQ
                     GDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVAFLISPVKNSDGIFYDSRNY
                     EAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTSV
                     KH"
     misc_feature    3934..3936
                     /label=D156R
     misc_feature    4171..4173
                     /label=D235R
     misc_feature    4342..4344
                     /label=E292R
     misc_feature    4516..4518
                     /label=D350R
     CDS             7161..7869
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMR
                     IKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDM
                     ALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVA
                     RYCDLPSKLGHKLN"
     primer_bind     7161..7178
                     /label=BFP-F-AgeI
     primer_bind     complement(7850..7869)
                     /label=BFP-R-XhoI
     CDS             7876..7923
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7942..8007
                     /codon_start=1
                     /product="three tandem FLAG epitope tags, followed by an 
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    8014..8581
                     /label=IRES
     primer_bind     complement(8029..8052)
                     /label=PQCXIP-R (=pQC-R)
     primer_bind     complement(8054..8073)
                     /label=pIRES2-EGFP-P3
     primer_bind     complement(8103..8122)
                     /label=IRESrev
     primer_bind     8456..8476
                     /label=IRES-F
     CDS             8601..9200
                     /codon_start=1
                     /label=PuroR
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    9219..9807
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9254..9276)
                     /label=PCDNA3-4-R
     primer_bind     complement(9272..9292)
                     /label=WPRE-R
     primer_bind     complement(9295..9312)
                     /label=GIPZ sequencing primer
     CDS             complement(9690..9701)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9882..10115
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     9973..9992
                     /label=PCDNA3.0-F2
     primer_bind     9975..9995
                     /label=PCDNA3.1-F
     primer_bind     complement(10234..10252)
                     /label=pBRforEco
     primer_bind     complement(10286..10305)
                     /label=PBR322F
     primer_bind     complement(10302..10321)
                     /label=PTRG-R
     promoter        10320..10424
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10380..10398)
                     /label=BLA-Prorev
     CDS             10425..11285
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10731..10753
                     /label=AmpR-1F
     primer_bind     complement(10956..10974)
                     /label=AmpR-1R
     primer_bind     11178..11201
                     /label=PrimerBlaM1
     primer_bind     11178..11201
                     /label=Primer Bla M1
     primer_bind     complement(11383..11406)
                     /label=PrimerBlaRV
     primer_bind     complement(11383..11406)
                     /label=Primer Bla RV
     rep_origin      11456..12044
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12231..12243
                     /label=PCAMBIA2300-5
     primer_bind     12307..12326
                     /label=SV40pro-F
     primer_bind     complement(12330..12341)
                     /label=SV40pro-F
     primer_bind     12362..12379
                     /label=SV40ori-3for
     protein_bind    12455..12476
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     12485..12504
                     /label=PCAMBIA1301-F(即为P1)
     primer_bind     12486..12507
                     /label=SRprimer
     primer_bind     12488..12507
                     /label=ptarget-R
     promoter        12491..12521
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     12505..12524
                     /label=Y-M13R-48
     primer_bind     12512..12529
                     /label=PCAMBIA1300-5
     protein_bind    12529..12545
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12532..12551
                     /label=pQE30-F
     primer_bind     12534..12557
                     /label=M13R-48
     primer_bind     12534..12555
                     /label=PQ60-F
     primer_bind     12553..12569
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12553..12569
                     /label=pDONR222-R
     primer_bind     12553..12569
                     /label=M13Rev(-26)
     primer_bind     12560..12578
                     /label=M13mp18-R
     primer_bind     12604..12623
                     /label=PIRES-R
     primer_bind     12609..12628
                     /label=PCI-NEO-R
     primer_bind     12619..12638
                     /label=EBVrev
     primer_bind     12627..12646
                     /label=pCMV-R
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     2581 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     2641 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     2701 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     2761 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     2821 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     2881 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     2941 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     3001 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     3061 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     3121 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     3181 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     3241 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     3301 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     3361 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaagcgg ccgcatgaag
     3421 cgacctgccg ccacaaagaa ggctggacag gctaagaaga agaaagctag ctcaaagctg
     3481 gagaaattca ccaactgtta tagcctgtct aagaccctgc gcttcaaggc aatcccagtg
     3541 ggcaagacac aagagaacat tgacaacaaa cggctcctgg tggaggatga gaagagggct
     3601 gaagattaca agggcgttaa gaagctgctg gataggtact atctgtcatt catcaacgat
     3661 gtcctccaca gtatcaagct gaagaatctg aacaattaca tttctctgtt ccggaagaag
     3721 acacggaccg agaaggagaa caaagagctg gagaatctgg agatcaacct gaggaaagaa
     3781 atagctaagg ctttcaaagg gaacgagggt tacaagtccc tgttcaagaa agacattatc
     3841 gagactattc tgcctgagtt cctggacgat aaagatgaga tcgccctcgt caattccttc
     3901 aatgggttta ccacagcctt taccggcttc ttcaggaata gagagaatat gttctctgaa
     3961 gaggccaaat ccactagcat cgcctttcgc tgcataaacg agaacctgac taggtacatc
     4021 agcaatatgg acatctttga gaaagtcgat gccatattcg acaaacatga ggtgcaggag
     4081 attaaggaga agatcctgaa ctcagattac gatgtcgaag atttcttcga gggagagttc
     4141 ttcaacttcg tgctcacaca agagggcatt agggtgtaca atgcaatcat tggagggttc
     4201 gtgacagaga gtggcgagaa gataaagggc ctgaacgagt atatcaacct ctacaaccag
     4261 aaaaccaagc agaaactgcc taagttcaag ccactgtaca aacaagtgct ctcagatagg
     4321 gaaagcctga gcttctacgg tagggggtat acatcagatg aagaagtgct cgaagtgttc
     4381 cgcaacaccc tcaataagaa cagtgaaatc ttctcttcaa tcaagaagct ggagaaactg
     4441 ttcaagaatt tcgatgagta ctcctctgcc ggaatctttg tgaagaatgg ccctgcaata
     4501 tccactatta gcaaaaggat ctttggcgag tggaacgtta tcagggataa gtggaatgcc
     4561 gagtacgatg atattcatct caagaagaaa gccgtggtta cagagaaata cgaggatgat
     4621 agacgcaaga gctttaagaa gattggtagc ttctctctcg aacagctgca ggagtacgcc
     4681 gacgctgacc tgtcagtcgt ggagaaactc aaggagatca taatccagaa ggtggatgaa
     4741 atctacaaag tgtatggaag ctctgagaaa ctcttcgatg cagactttgt tctggagaag
     4801 agtctgaaga agaacgacgc agtggttgct atcatgaagg acctgctgga ttctgttaag
     4861 tctttcgaga attacattaa ggcattcttt ggtgaaggga aggagacaaa tagggacgag
     4921 agcttctatg gcgactttgt tctggcctac gacatcctcc tcaaggttga ccacatctat
     4981 gacgctatac ggaattacgt tacccagaag ccctatagca aagacaagtt caagctgtat
     5041 ttccagaatc cacagtttat gggtgggtgg gataaagaca aagaaacaga ttacagggcc
     5101 actatcctgc ggtacggcag caaatactat ctggctatca tggataagaa gtacgccaaa
     5161 tgcctccaga agatcgacaa ggacgacgtg aacggtaact acgagaagat caattacaag
     5221 ctcctgccag gacctaacaa gatgctgccc aaggtgttct tctccaagaa atggatggcc
     5281 tactataacc caagcgagga cattcagaag atatacaaga atgggacatt caagaagggc
     5341 gatatgttca acctcaacga ctgccacaag ctgattgatt tcttcaagga tagcatttct
     5401 cgctatccca agtggtctaa tgcatacgat ttcaacttca gcgagactga gaagtacaaa
     5461 gacatcgctg gcttctaccg ggaggtggaa gagcaaggct ataaggtgtc attcgaatcc
     5521 gcttctaaga aggaagtgga taagctcgtg gaagagggta agctgtacat gttccagata
     5581 tacaacaaag acttcagcga taagagccac ggcactccaa acctccatac tatgtatttc
     5641 aagctgctgt ttgacgagaa caaccacgga cagattaggc tgtcaggagg cgcagaactc
     5701 ttcatgcgca gagcttcact gaagaaggag gaactcgttg tccacccagc caatagccct
     5761 atagccaata agaatccaga caatcctaag aaaaccacta ctctgtctta cgatgtgtat
     5821 aaggataaga gattctctga agatcagtac gaactgcaca tacccattgc cattaacaag
     5881 tgccctaaga acatcttcaa gattaacaca gaggttagag tgctcctgaa acacgacgat
     5941 aacccttatg ttataggcat tgctcgcgga gagagaaacc tgctgtacat cgtcgtggtg
     6001 gacggcaaag gcaacatcgt ggaacagtac agtctcaatg aaatcattaa caatttcaac
     6061 ggaatccgca ttaagaccga ctaccattct ctcctcgaca agaaggagaa agaaaggttc
     6121 gaagcaagac agaattggac aagtatagag aatatcaaag aactgaaggc tgggtacatc
     6181 tctcaggttg tgcacaagat atgtgagctg gtggagaagt acgacgctgt tatcgccctc
     6241 gcggacctga atagcggctt caagaactcc agggtgaagg tggagaagca ggtgtatcag
     6301 aagttcgaga agatgctgat cgacaagctc aactatatgg tggacaagaa atccaatcct
     6361 tgcgctactg gtggagccct gaagggctat caaatcacca ataagttcga atctttcaag
     6421 tctatgagca cccagaatgg cttcatcttc tacatacccg catggctgac atccaagatt
     6481 gatccctcta ccggatttgt taatctgctc aagactaagt acacctctat tgctgactca
     6541 aagaagttca tatcatcatt tgaccgcatc atgtacgtgc cagaagagga cctgttcgag
     6601 tttgccctgg attacaagaa tttctctcgg actgacgccg actacatcaa gaagtggaag
     6661 ctctactctt atggtaatcg gattcgcata ttccgcaatc ccaagaagaa taacgtgttc
     6721 gattgggagg aagtttgcct caccagcgct tacaaggagc tgttcaataa gtatgggatt
     6781 aactaccagc agggcgacat aagagccctg ctgtgcgaac aatctgataa ggcattctat
     6841 tcctctttca tggcactgat gtcactgatg ctgcaaatgc gcaattccat caccggaaga
     6901 acagacgtgg cctttctgat ctctcctgtc aagaactcag atggcatctt ctacgattcc
     6961 cgcaactatg aagcacagga gaatgctatc ctgcctaaga atgccgatgc aaatggagcc
     7021 tataacatcg ccagaaaggt cctctgggcc ataggacaat tcaagaaagc tgaagatgag
     7081 aagctggaca aggtgaagat cgccatttca aacaaagagt ggctcgaata tgctcagacc
     7141 tcagtgaagc atagtctaga ggtgtctaag ggcgaagagc tgattaagga gaacatgcac
     7201 atgaagctgt acatggaggg caccgtggac aaccatcact tcaagtgcac atccgagggc
     7261 gaaggcaagc cctacgaggg cacccagacc atgagaatca aggtggtcga gggcggccct
     7321 ctccccttcg ccttcgacat cctggctact agcttcctct acggcagcaa gaccttcatc
     7381 aaccacaccc agggcatccc cgacttcttc aagcagtcct tccctgaggg cttcacatgg
     7441 gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga caccagcctc
     7501 caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttcac atccaacggc
     7561 cctgtgatgc agaagaaaac actcggctgg gaggccttca ccgagacgct gtaccccgct
     7621 gacggcggcc tggaaggcag aaacgacatg gccctgaagc tcgtgggcgg gagccatctg
     7681 atcgcaaacg ccaagaccac atatagatcc aagaaacccg ctaagaacct caagatgcct
     7741 ggcgtctact atgtggacta cagactggaa agaatcaagg aggccaacaa cgaaacctac
     7801 gtcgagcagc acgaggtggc agtggccaga tactgcgacc tccctagcaa actggggcac
     7861 aagcttaatc atatgaagcg acctgccgcc acaaagaagg ctggacaggc taagaagaag
     7921 aaacaacgcg tgcagctaag cgactacaaa gaccatgacg gtgattataa agatcatgac
     7981 atcgattaca aggatgacga tgacaagtaa ggatccctcc ccccccccta acgttactgg
     8041 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
     8101 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
     8161 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     8221 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     8281 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
     8341 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
     8401 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
     8461 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
     8521 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata
     8581 aggatccacc ggagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     8641 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     8701 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     8761 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     8821 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     8881 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     8941 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     9001 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     9061 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     9121 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     9181 cccgcaagcc cggtgcctga cgcccatcga tagatcctaa tcaacctctg gattacaaaa
     9241 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     9301 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     9361 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     9421 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     9481 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     9541 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     9601 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     9661 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     9721 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     9781 ggatctccct ttgggccgcc tccccgcctg agatccttta agaccaatga cttacaaggc
     9841 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     9901 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     9961 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
    10021 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
    10081 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
    10141 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcccg ggttaattaa
    10201 ggaaagggct agatcattct tgaagacgaa agggcctcgt gatacgccta tttttatagg
    10261 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
    10321 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
    10381 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
    10441 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
    10501 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
    10561 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
    10621 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc
    10681 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
    10741 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
    10801 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
    10861 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
    10921 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
    10981 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
    11041 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
    11101 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
    11161 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
    11221 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
    11281 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
    11341 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
    11401 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
    11461 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
    11521 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
    11581 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
    11641 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
    11701 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
    11761 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
    11821 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
    11881 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
    11941 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
    12001 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    12061 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
    12121 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
    12181 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
    12241 aaccgcctct ccccgcgcgt tggccgattc attaatgcag caagctcatg gctgactaat
    12301 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
    12361 aggaggcttt tttggaggcc taggcttttg caaaaagctc cccgtggcac gacaggtttc
    12421 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
    12481 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
    12541 aacaatttca cacaggaaac agctatgaca tgattacgaa tttcacaaat aaagcatttt
    12601 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
    12661 tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta
    12721 ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt
    12781 cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagt
//
