LOCUS       Exported               12136 bp ds-DNA     circular SYN 01-DEC-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12136)
  AUTHORS   Triple Threat
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Mar 13, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12136
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     492..511
                     /label=PCDNA3.0-F2
     primer_bind     494..514
                     /label=PCDNA3.1-F
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     complement(2076..2095)
                     /label=PLKO1-R
     primer_bind     2088..2111
                     /label=U6-pll3-7
     promoter        2224..3405
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     primer_bind     complement(2296..2318)
                     /label=pCDH1-R
     primer_bind     complement(2341..2360)
                     /label=EF1A-R
     intron          2454..3396
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3353..3373
                     /label=EF-1aFwd(PEF-F)
     CDS             3418..3465
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             3472..7152
                     /codon_start=1
                     /label=dLbCas12a
                     /translation="SKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAED
                     YKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKEI
                     AKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFRNRENMFSE
                     EAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEGE
                     FFNFVLTQEGIRVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVLS
                     DRESLSFYGRGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKNG
                     PAISTISKRIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQL
                     QEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKDL
                     LDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYSK
                     DKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNGN
                     YEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKLI
                     DFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLVE
                     EGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKKE
                     ELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKIN
                     TEVRVLLKHDDNPYVIGIARGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTDY
                     HSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALADLNSGF
                     KNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQN
                     GFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALDY
                     KNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQQ
                     GDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVAFLISPVKNSDGIFYDSRNY
                     EAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTSV
                     KH"
     misc_feature    3934..3936
                     /label=D156R
     misc_feature    4171..4173
                     /label=D235R
     misc_feature    4342..4344
                     /label=E292R
     misc_feature    4516..4518
                     /label=D350R
     CDS             7183..7230
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7249..7314
                     /codon_start=1
                     /product="three tandem FLAG epitope tags, followed by an 
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    7321..7888
                     /label=IRES
     primer_bind     complement(7336..7359)
                     /label=PQCXIP-R (=pQC-R)
     primer_bind     complement(7361..7380)
                     /label=pIRES2-EGFP-P3
     primer_bind     complement(7410..7429)
                     /label=IRESrev
     primer_bind     7763..7783
                     /label=IRES-F
     CDS             7908..8507
                     /codon_start=1
                     /label=PuroR
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    8526..9114
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8561..8583)
                     /label=PCDNA3-4-R
     primer_bind     complement(8579..8599)
                     /label=WPRE-R
     primer_bind     complement(8602..8619)
                     /label=GIPZ sequencing primer
     CDS             complement(8997..9008)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9189..9422
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     9280..9299
                     /label=PCDNA3.0-F2
     primer_bind     9282..9302
                     /label=PCDNA3.1-F
     primer_bind     complement(9541..9559)
                     /label=pBRforEco
     primer_bind     complement(9593..9612)
                     /label=PBR322F
     primer_bind     complement(9609..9628)
                     /label=PTRG-R
     promoter        9627..9731
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9687..9705)
                     /label=BLA-Prorev
     CDS             9732..10592
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10038..10060
                     /label=AmpR-1F
     primer_bind     complement(10263..10281)
                     /label=AmpR-1R
     primer_bind     10485..10508
                     /label=PrimerBlaM1
     primer_bind     10485..10508
                     /label=Primer Bla M1
     primer_bind     complement(10690..10713)
                     /label=PrimerBlaRV
     primer_bind     complement(10690..10713)
                     /label=Primer Bla RV
     rep_origin      10763..11351
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11538..11550
                     /label=PCAMBIA2300-5
     primer_bind     11614..11633
                     /label=SV40pro-F
     primer_bind     complement(11637..11648)
                     /label=SV40pro-F
     primer_bind     11669..11686
                     /label=SV40ori-3for
     protein_bind    11762..11783
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     11792..11811
                     /label=PCAMBIA1301-F(即为P1)
     primer_bind     11793..11814
                     /label=SRprimer
     primer_bind     11795..11814
                     /label=ptarget-R
     promoter        11798..11828
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     11812..11831
                     /label=Y-M13R-48
     primer_bind     11819..11836
                     /label=PCAMBIA1300-5
     protein_bind    11836..11852
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11839..11858
                     /label=pQE30-F
     primer_bind     11841..11864
                     /label=M13R-48
     primer_bind     11841..11862
                     /label=PQ60-F
     primer_bind     11860..11876
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11860..11876
                     /label=pDONR222-R
     primer_bind     11860..11876
                     /label=M13Rev(-26)
     primer_bind     11867..11885
                     /label=M13mp18-R
     primer_bind     11911..11930
                     /label=PIRES-R
     primer_bind     11916..11935
                     /label=PCI-NEO-R
     primer_bind     11926..11945
                     /label=EBVrev
     primer_bind     11934..11953
                     /label=pCMV-R
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     2581 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     2641 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     2701 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     2761 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     2821 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     2881 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     2941 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     3001 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     3061 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     3121 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     3181 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     3241 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     3301 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     3361 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaagcgg ccgcatgaag
     3421 cgacctgccg ccacaaagaa ggctggacag gctaagaaga agaaagctag ctcaaagctg
     3481 gagaaattca ccaactgtta tagcctgtct aagaccctgc gcttcaaggc aatcccagtg
     3541 ggcaagacac aagagaacat tgacaacaaa cggctcctgg tggaggatga gaagagggct
     3601 gaagattaca agggcgttaa gaagctgctg gataggtact atctgtcatt catcaacgat
     3661 gtcctccaca gtatcaagct gaagaatctg aacaattaca tttctctgtt ccggaagaag
     3721 acacggaccg agaaggagaa caaagagctg gagaatctgg agatcaacct gaggaaagaa
     3781 atagctaagg ctttcaaagg gaacgagggt tacaagtccc tgttcaagaa agacattatc
     3841 gagactattc tgcctgagtt cctggacgat aaagatgaga tcgccctcgt caattccttc
     3901 aatgggttta ccacagcctt taccggcttc ttcaggaata gagagaatat gttctctgaa
     3961 gaggccaaat ccactagcat cgcctttcgc tgcataaacg agaacctgac taggtacatc
     4021 agcaatatgg acatctttga gaaagtcgat gccatattcg acaaacatga ggtgcaggag
     4081 attaaggaga agatcctgaa ctcagattac gatgtcgaag atttcttcga gggagagttc
     4141 ttcaacttcg tgctcacaca agagggcatt agggtgtaca atgcaatcat tggagggttc
     4201 gtgacagaga gtggcgagaa gataaagggc ctgaacgagt atatcaacct ctacaaccag
     4261 aaaaccaagc agaaactgcc taagttcaag ccactgtaca aacaagtgct ctcagatagg
     4321 gaaagcctga gcttctacgg tagggggtat acatcagatg aagaagtgct cgaagtgttc
     4381 cgcaacaccc tcaataagaa cagtgaaatc ttctcttcaa tcaagaagct ggagaaactg
     4441 ttcaagaatt tcgatgagta ctcctctgcc ggaatctttg tgaagaatgg ccctgcaata
     4501 tccactatta gcaaaaggat ctttggcgag tggaacgtta tcagggataa gtggaatgcc
     4561 gagtacgatg atattcatct caagaagaaa gccgtggtta cagagaaata cgaggatgat
     4621 agacgcaaga gctttaagaa gattggtagc ttctctctcg aacagctgca ggagtacgcc
     4681 gacgctgacc tgtcagtcgt ggagaaactc aaggagatca taatccagaa ggtggatgaa
     4741 atctacaaag tgtatggaag ctctgagaaa ctcttcgatg cagactttgt tctggagaag
     4801 agtctgaaga agaacgacgc agtggttgct atcatgaagg acctgctgga ttctgttaag
     4861 tctttcgaga attacattaa ggcattcttt ggtgaaggga aggagacaaa tagggacgag
     4921 agcttctatg gcgactttgt tctggcctac gacatcctcc tcaaggttga ccacatctat
     4981 gacgctatac ggaattacgt tacccagaag ccctatagca aagacaagtt caagctgtat
     5041 ttccagaatc cacagtttat gggtgggtgg gataaagaca aagaaacaga ttacagggcc
     5101 actatcctgc ggtacggcag caaatactat ctggctatca tggataagaa gtacgccaaa
     5161 tgcctccaga agatcgacaa ggacgacgtg aacggtaact acgagaagat caattacaag
     5221 ctcctgccag gacctaacaa gatgctgccc aaggtgttct tctccaagaa atggatggcc
     5281 tactataacc caagcgagga cattcagaag atatacaaga atgggacatt caagaagggc
     5341 gatatgttca acctcaacga ctgccacaag ctgattgatt tcttcaagga tagcatttct
     5401 cgctatccca agtggtctaa tgcatacgat ttcaacttca gcgagactga gaagtacaaa
     5461 gacatcgctg gcttctaccg ggaggtggaa gagcaaggct ataaggtgtc attcgaatcc
     5521 gcttctaaga aggaagtgga taagctcgtg gaagagggta agctgtacat gttccagata
     5581 tacaacaaag acttcagcga taagagccac ggcactccaa acctccatac tatgtatttc
     5641 aagctgctgt ttgacgagaa caaccacgga cagattaggc tgtcaggagg cgcagaactc
     5701 ttcatgcgca gagcttcact gaagaaggag gaactcgttg tccacccagc caatagccct
     5761 atagccaata agaatccaga caatcctaag aaaaccacta ctctgtctta cgatgtgtat
     5821 aaggataaga gattctctga agatcagtac gaactgcaca tacccattgc cattaacaag
     5881 tgccctaaga acatcttcaa gattaacaca gaggttagag tgctcctgaa acacgacgat
     5941 aacccttatg ttataggcat tgctcgcgga gagagaaacc tgctgtacat cgtcgtggtg
     6001 gacggcaaag gcaacatcgt ggaacagtac agtctcaatg aaatcattaa caatttcaac
     6061 ggaatccgca ttaagaccga ctaccattct ctcctcgaca agaaggagaa agaaaggttc
     6121 gaagcaagac agaattggac aagtatagag aatatcaaag aactgaaggc tgggtacatc
     6181 tctcaggttg tgcacaagat atgtgagctg gtggagaagt acgacgctgt tatcgccctc
     6241 gcggacctga atagcggctt caagaactcc agggtgaagg tggagaagca ggtgtatcag
     6301 aagttcgaga agatgctgat cgacaagctc aactatatgg tggacaagaa atccaatcct
     6361 tgcgctactg gtggagccct gaagggctat caaatcacca ataagttcga atctttcaag
     6421 tctatgagca cccagaatgg cttcatcttc tacatacccg catggctgac atccaagatt
     6481 gatccctcta ccggatttgt taatctgctc aagactaagt acacctctat tgctgactca
     6541 aagaagttca tatcatcatt tgaccgcatc atgtacgtgc cagaagagga cctgttcgag
     6601 tttgccctgg attacaagaa tttctctcgg actgacgccg actacatcaa gaagtggaag
     6661 ctctactctt atggtaatcg gattcgcata ttccgcaatc ccaagaagaa taacgtgttc
     6721 gattgggagg aagtttgcct caccagcgct tacaaggagc tgttcaataa gtatgggatt
     6781 aactaccagc agggcgacat aagagccctg ctgtgcgaac aatctgataa ggcattctat
     6841 tcctctttca tggcactgat gtcactgatg ctgcaaatgc gcaattccat caccggaaga
     6901 acagacgtgg cctttctgat ctctcctgtc aagaactcag atggcatctt ctacgattcc
     6961 cgcaactatg aagcacagga gaatgctatc ctgcctaaga atgccgatgc aaatggagcc
     7021 tataacatcg ccagaaaggt cctctgggcc ataggacaat tcaagaaagc tgaagatgag
     7081 aagctggaca aggtgaagat cgccatttca aacaaagagt ggctcgaata tgctcagacc
     7141 tcagtgaagc atagtctaga ggggggagga ggtagtcata tgaagcgacc tgccgccaca
     7201 aagaaggctg gacaggctaa gaagaagaaa caacgcgtgc agctaagcga ctacaaagac
     7261 catgacggtg attataaaga tcatgacatc gattacaagg atgacgatga caagtaagga
     7321 tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
     7381 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
     7441 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
     7501 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
     7561 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
     7621 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
     7681 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
     7741 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg
     7801 ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt
     7861 ggttttcctt tgaaaaacac gatgataagg atccaccgga gcttaccatg accgagtaca
     7921 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     7981 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     8041 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     8101 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     8161 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     8221 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     8281 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     8341 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     8401 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     8461 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc ccatcgatag
     8521 atcctaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     8581 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     8641 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     8701 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     8761 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     8821 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     8881 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     8941 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     9001 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     9061 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctgaga
     9121 tcctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     9181 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     9241 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     9301 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     9361 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     9421 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     9481 agagagtgag aggcccgggt taattaagga aagggctaga tcattcttga agacgaaagg
     9541 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     9601 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     9661 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     9721 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     9781 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     9841 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     9901 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     9961 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
    10021 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
    10081 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
    10141 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
    10201 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
    10261 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
    10321 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
    10381 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
    10441 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
    10501 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
    10561 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
    10621 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
    10681 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
    10741 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
    10801 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
    10861 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
    10921 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
    10981 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
    11041 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
    11101 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    11161 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    11221 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    11281 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    11341 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
    11401 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
    11461 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
    11521 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
    11581 aatgcagcaa gctcatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
    11641 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
    11701 aaagctcccc gtggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
    11761 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
    11821 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga
    11881 ttacgaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
    11941 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc
    12001 ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt catttactct
    12061 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
    12121 ctacaaactt agtagt
//
