LOCUS       pMXs-8XHis-mCher        6499 bp    DNA     circular SYN 13-MAR-2025
DEFINITION  pMX_8x_His_1.
ACCESSION   pMX_8x_His_1
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6499)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 13, 2025 from SnapGene 8.0.2
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
COMMENT     Sequence Label: pMXs-8XHis-mCherry-Omp25
FEATURES             Location/Qualifiers
     source          1..6499
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             772..824
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=4.9"
                     /note="/other=CDS"
                     /translation="APNTQTASPRALADSLM"
     LTR             complement(890..1359)
                     /label=LTR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
     LTR             complement(1360..1481)
                     /label=3' LTR (Delta-U3) (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.6"
                     /note="/match_length=28.9"
                     /note="/other=LTR"
     CDS             complement(1530..1919)
                     /codon_start=1
                     /label=BSD
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=97.7"
                     /note="/other=CDS"
                     /translation="PLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGVNV
                     YHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIKAI
                     VKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    complement(1939..2401)
                     /label=IRES
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     promoter        2509..2525
                     /label=chicken beta-actin promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=6.6"
                     /note="/other=promoter"
     CDS             complement(2547..2657)
                     /codon_start=1
                     /label=Synj2bp (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=25.5"
                     /note="/other=CDS"
                     /translation="HRGDGEPSGVPVAVVLLPVFALTLVAVWAFVRYRKQL"
     CDS             complement(2664..3368)
                     /codon_start=1
                     /label=mCherry
                     /note="pLannotate"
                     /note="/database=fpbase"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.6"
                     /note="/other=CDS"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     CDS             complement(3381..3404)
                     /codon_start=1
                     /label=8xHis
                     /label=7xHis
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="HHHHHHHH"
     misc_feature    complement(3469..3843)
                     /label=pol region
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             complement(3853..4269)
                     /codon_start=1
                     /label=gag (truncated)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="GQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTF
                     NVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKP
                     PPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA"
     misc_feature    complement(4346..4434)
                     /label=MESV Psi (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=98.9"
                     /note="/match_length=26.0"
                     /note="/other=misc_feature"
     misc_feature    complement(4486..4685)
                     /label=MMLV Psi
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     LTR             complement(4748..5341)
                     /label=LTR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
     promoter        5371..5475
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             5476..6336
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaactatg gccatttaat
      841 gtaaatactt aagaaaaaaa accaaattaa ttttgataca tgctgcatgt gaaagacccc
      901 cgctgacggg tagtcaatca ctcagaggag accctcccaa ggaacagcga gaccacaagt
      961 cggatgcaac tgcaagaggg tttattggat acacgggtac ccgggcgact cagtcaatcg
     1021 gaggactggc gccccgagtg aggggttgtg ggctctttta ttgagctcgg ggagcagaag
     1081 cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc aaataaggca cagggtcatt
     1141 tcaggtcctt ggggcaccct ggaaacatct gatggttctc tagaaactgc tgagggctgg
     1201 accgcatctg gggaccatct gttcttggcc ctgagccggg gcaggaactg cttaccacag
     1261 atatcctgtt tggcccatat tcagctgttc catctgttct tggccctgag ccggggcagg
     1321 aactgcttac cacagatatc ctgtttggcc catattcagc tgttccatct gttcctgacc
     1381 ttgatctgaa cttctctatt ctcagttatg tatttttcca tgccttgcaa aatggcgtta
     1441 cttaagctag cttgccaaac ctacaggtgg ggtctttcat tccccccttt ttctggagac
     1501 taaataaaat cttttatttt atcgtcgact tagccctccc acacataacc agagggcagc
     1561 aattcacgaa tcccaactgc cgtcggctgt ccatcactgt ccttcactat ggctttgatc
     1621 ccaggatgca gatcgagaag cacctgtcgg caccgtccgc aggggctcaa gatgcccctg
     1681 ttctcatttc cgatcgcgac gatacaagtc aggttgccag ctgccgcagc agcagcagtg
     1741 cccagcacca cgagttctgc acaaggtccc ccagtaaaat gatatacatt gacaccagtg
     1801 aagatgcggc cgtcgctaga gagagctgcg ctggcgacgc tgtagtcttc agagatgggg
     1861 atgctgttga ttgtagccgt tgctctttca atgagggtgg attcttcttg agacaaagga
     1921 accatggttg tggccatatt atcatcgtgt ttttcaaagg aaaaccacgt ccccgtggtt
     1981 cggggggcct agacgttttt ttaacctcga ctaaacacat gtaaagcatg tgcaccgagg
     2041 ccccagatca gatcccatac aatggggtac cttctgggca tccttcagcc ccttgttgaa
     2101 tacgcttgag gagagccatt tgactctttc cacaactatc caactcacaa cgtggcactg
     2161 gggttgtgcc gcctttgcag gtgtatctta tacacgtggc ttttggccgc agaggcacct
     2221 gtcgccaggt ggggggttcc gctgcctgca aagggtcgct acagacgttg tttgtcttca
     2281 agaagcttcc agaggaactg cttccttcac gacattcaac agaccttgca ttcctttggc
     2341 gagaggggaa agacccctag gaatgctcgt caagaagaca gggccaggtt tccgggccct
     2401 cacattgcca aaagacggca atatggtgga aaataacata tagacaaacg cacaccggcc
     2461 ttattccaag cggcttcggc cagtaacgtt aggggggggg gagggagagg gggggggggg
     2521 ggcggaattt acgtagcggc cgctcagagc tgctttcggt atctcacgaa ggcccaaact
     2581 gctaccaggg taagggcaaa cactggcagc agcaccacag ctacaggaac tccactaggc
     2641 tctccgtcgc ctcgatgccg cggcttgtac agctcgtcca tgccgccggt ggagtggcgg
     2701 ccctcggcgc gttcgtactg ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc
     2761 aacttgatgt tgacgttgta ggcgccgggc agctgcacgg gcttcttggc cttgtaggtg
     2821 gtcttgacct cagcgtcgta gtggccgccg tccttcagct tcagcctctg cttgatctcg
     2881 cccttcaggg cgccgtcctc ggggtacatc cgctcggagg aggcctccca gcccatggtc
     2941 ttcttctgca ttacggggcc gtcggagggg aagttggtgc cgcgcagctt caccttgtag
     3001 atgaactcgc cgtcctgcag ggaggagtcc tgggtcacgg tcaccacgcc gccgtcctcg
     3061 aagttcatca cgcgctccca cttgaagccc tcggggaagg acagcttcaa gtagtcgggg
     3121 atgtcggcgg ggtgcttcac gtaggccttg gagccgtaca tgaactgagg ggacaggatg
     3181 tcccaggcga agggcagggg gccacccttg gtcaccttca gcttggcggt ctgggtgccc
     3241 tcgtaggggc ggccctcgcc ctcgccctcg atctcgaact cgtggccgtt cacggagccc
     3301 tccatgtgca ccttgaagcg catgaactcc ttgatgatgg ccatgttatc ctcctcgccc
     3361 ttgctcacgc cgctccctcc gtgatggtga tggtgatggt gatgctcgag gcctgcagga
     3421 attcccgtac caccacactg ggatccttaa ttaactagct agatccggca gtctagagga
     3481 tggtccaccc ccggggtcgg cagccttcac gtgggcggcg tgtatccaag ctgcgatgcc
     3541 gtctactttg agggcggtgg gggtggtcag caggactgtg taaggtcctt tccagcgagg
     3601 ttctaggttc ttagtctggt gtcggcggac ccacactgtg tcgccgactc ggtaagggtg
     3661 aggtaccacc ggtcggtcca gttgttcttg gtaggctgcc gccagaggtc tccagacttc
     3721 gtgctggact aagtagagag cctgtaagtg agcttggaga gaggggctgt tagtaactct
     3781 tgtcatgtca gggtcaggga agtttacaag gggcgggggt gccccatata agatctcata
     3841 tggccatatg ggggcgccta gagaaggagt gagggctgga taaagggagg atcgaggcgg
     3901 ggtcgaacga ggaggttcaa gggggagaga cggggcggat ggaggaagag gaggcggagg
     3961 cttagggtgt acaaagggct tgacccaggg aggggggtca aaagccaagg cttcccaggt
     4021 cacgatgtag gggacctggt ctgggtgtcc atgcgggcca ggtgaaaaga ccttgatctt
     4081 aacctgggtg atgaggtctc ggttaaaggt gccgtctcgc ggccatccga cgttaaaggt
     4141 tggccattct gcagagcaga aggtaaccca acgtctcttc ttgacatcta ccgactggtt
     4201 gtgagcgatc cgctcgacat ctttccagtg acctaaggtc aaacttaagg gagtggtaac
     4261 agtctggccc taattttcag acaaatacag aaacacagtc agacagagac aacacagaac
     4321 gatgctgcag cagacaagac gcgcggcgcg gcttcggtcc caaaccgaaa gcaaaaattc
     4381 agacggaggc gggaactgtt ttaggttctc gtctcctacc agaaccacat atccctccta
     4441 ttaggggggt gcaccaaaga gtccaaaacg atcgggattt ttggactcag gtcgggccac
     4501 aaaaacggcc cccgaagtcc ctgggacgtc tcccagggtt gcggccgggt gttccgaact
     4561 cgtcagttcc accacgggtc cgccagatac agagctagtt agctaactag taccgacgca
     4621 ggcgcataaa atcagtcata gacactagac aatcggacag acacagataa gttgctggcc
     4681 agcttacctc ccggtggtgg gtcggtggtc cctgggcagg ggtctcccga tcccggacga
     4741 gcccccaaat gaaagacccc cgctgacggg tagtcaatca ctcagaggag accctcccaa
     4801 ggaacagcga gaccacaagt cggatgcaac tgcaagaggg tttattggat acacgggtac
     4861 ccgggcgact cagtcaatcg gaggactggc gcgccgagtg aggggttgtg ggctctttta
     4921 ttgagctcgg ggagcagaag cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc
     4981 aaataaggca cagggtcatt tcaggtcctt ggggcaccct ggaaacatct gatggttctc
     5041 tagaaactgc tgagggctgg accgcatctg gggaccatct gttcttggcc ctgagccggg
     5101 gcaggaactg cttaccacag atatcctgtt tggcccatat tcagctgttc catctgttct
     5161 tggccctgag ccggggcagg aactgcttac cacagatatc ctgtttggcc catattcagc
     5221 tgttccatct gttcctgacc ttgatctgaa cttttctatt ctcagttatg tatttttcca
     5281 tgccttgcaa aatggcgtta cttaagctag cttgccaaac ctacaggtgg ggtctttcat
     5341 tatgcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     5401 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     5461 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     5521 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     5581 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     5641 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     5701 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     5761 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     5821 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     5881 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     5941 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     6001 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     6061 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     6121 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     6181 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     6241 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     6301 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     6361 atactttaga ttgatttgcg gccggccgca aaaggatcta ggtgaagatc ctttttgata
     6421 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     6481 aaaagatcaa aggatcttc
//
