LOCUS       Exported                4577 bp ds-DNA     circular SYN 12-MAR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 14, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.1
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      466..653
                     /label=ADH1 terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     primer_bind     654..676
                     /label=pAPDel_F
     misc_feature    677..720
                     /label=H2Bp (missing 22bp)
     misc_feature    721..937
                     /label=Shared promoter region
     misc_feature    938..1055
                     /label=H2Ap
     misc_feature    1062..1634
                     /label=NatR
     terminator      1640..1837
                     /label=TEF terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     primer_bind     complement(1838..1860)
                     /label=pAPInt_R
     promoter        complement(1881..1899)
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             complement(1921..1974)
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=5.0"
                     /note="/other=CDS"
                     /translation="APNTQTASPRALADSLMQ"
     rep_origin      complement(2157..2745)
                     /direction=LEFT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     misc_feature    complement(2916..3776)
                     /label=AmpR
     promoter        complement(3777..3881)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             3976..4011
                     /codon_start=1
                     /label=rop (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=18.8"
                     /note="/other=CDS"
                     /translation="LARFGDDGENL"
     misc_feature    4113..4164
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.9"
                     /note="/other=misc_feature"
     promoter        4227..4245
                     /label=SP6 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     primer_bind     4291..4310
                     /label=pAPInt_F
     CDS             join(4315..4577,1..445)
                     /codon_start=1
                     /label=mNeonGreen (codon optimized)
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
ORIGIN
        1 ggtcgacggc tctggctacc aagtccaccg caccatgcaa ttcgaggacg gcgcctctct
       61 cactgtcaac tacagataca cctacgaggg ctctcacatc aagggcgagg cccaagtcaa
      121 gggcaccggc ttccctgccg acggccctgt catgaccaac tctctcaccg ccgccgactg
      181 gtgccgctct aagaagacct accctaacga caagaccatc atctctacct tcaagtggtc
      241 ttacaccacc ggcaacggca agcgctaccg ctctaccgcc cgcaccacct acaccttcgc
      301 caagcctatg gccgccaact acctcaagaa ccaacctatg tacgtcttcc gcaagaccga
      361 gctcaagcac tctaagaccg agctcaactt caaggagtgg caaaaggcct tcaccgacgt
      421 catgggcatg gacgagctct acaagtgagc gccacttcta aataagcgaa tttcttatga
      481 tttatgattt ttattattaa ataagttata aaaaaaataa gtgtatacaa attttaaagt
      541 gactcttagg ttttaaaacg aaaattctta ttcttgagta actctttcct gtaggtcagg
      601 ttgctttctc aggtatagta tgaggtcgct cttattgacc acacctctac cggcagatcc
      661 gctagggata acagggtggt gcgggtgaaa ggtcttggtc aagtcgcaaa gtgtttgtga
      721 aaaagacgta gcgggggaag cgcacacacg cccgcctttt atagacgaag cttcgagcag
      781 gtcatttaac gatgcttagt agcaatatac aataaagcct aattgggact aattacggtc
      841 acgtgaaaaa ctcaccatgt agccttttat ggaaatattg tacacgctac taagccactg
      901 taatatcggc ctcatcgtct ccgcttggag cataaaaggc gacgctcctc gccattcccc
      961 caagtcgttt tccgtattct gctacgcttc aagctttgac gtttctcttc aagactcact
     1021 cacgaccctc tctctcacgc acaccttaca cagcagaatt catgggtacc actcttgacg
     1081 acacggctta ccggtaccgc accagtgtcc cgggggacgc cgaggccatc gaggcactgg
     1141 atgggtcctt caccaccgac accgtcttcc gcgtcaccgc caccggggac ggcttcaccc
     1201 tgcgggaggt gccggtggac ccgcccctga ccaaggtgtt ccccgacgac gaatcggacg
     1261 acgaatcgga cgacggggag gacggcgacc cggactcccg gacgttcgtc gcgtacgggg
     1321 acgacggcga cctggcgggc ttcgtggtcg tctcgtactc cggctggaac cgccggctga
     1381 ccgtcgagga catcgaggtc gccccggagc accgggggca cggggtcggg cgcgcgttga
     1441 tggggctcgc gacggagttc gcccgcgagc ggggcgccgg gcacctctgg ctggaggtca
     1501 ccaacgtcaa cgcaccggcg atccacgcgt accggcggat ggggttcacc ctctgcggcc
     1561 tggacaccgc cctgtacgac ggcaccgcct cggacggcga gcaggcgctc tacatgagca
     1621 tgccctgccc ctaatcagta ctgacaataa aaagattctt gttttcaaga acttgtcatt
     1681 tgtatagttt ttttatattg tagttgttct attttaatca aatgttagcg tgatttatat
     1741 tttttttcgc ctcgacatca tctgcccaga tgcgaagtta agtgcgcaga aagtaatatc
     1801 atgcgtcaat cgtatgtgaa tgctggtcgc tatactgcag atccactagt ggcctatgcg
     1861 gccgcggatc tgccggtctc cctatagtga gtcgtattaa tttcgataag ccaggttaac
     1921 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     1981 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     2041 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     2101 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     2161 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     2221 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     2281 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     2341 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     2401 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     2461 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     2521 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     2581 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     2641 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     2701 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     2761 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     2821 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     2881 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     2941 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     3001 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     3061 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     3121 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     3181 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     3241 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     3301 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     3361 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     3421 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     3481 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     3541 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     3601 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     3661 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     3721 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     3781 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     3841 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     3901 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     3961 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     4021 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     4081 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     4141 attgtactga gagtgcacca tatggacata ttgtcgttag aacgcggcta caattaatac
     4201 ataaccttat gtatcataca catacgattt aggtgacact atagaacgcg gccgccagct
     4261 gaagcttcgt acgctgcagg tcgacggatc ggtgacggtg ctggttctgg ttctatggtc
     4321 tctaagggcg aggaggacaa catggcctct ctccctgcca cccacgagct ccacatcttc
     4381 ggctctatca acggcgtcga cttcgacatg gtcggccaag gcaccggcaa ccctaacgac
     4441 ggctacgagg agctcaacct caagtctacc aagggcgacc tccaattctc tccttggatc
     4501 ctcgtccctc acatcggcta cggcttccac caatacctcc cttaccctga cggcatgtct
     4561 cctttccaag ccgccat
//
