LOCUS       Exported                4814 bp ds-DNA     circular SYN 12-MAR-2025
DEFINITION  mNeon-G418-1.
ACCESSION   mNeon-G418-1
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 14, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..4814
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             772..825
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=5.0"
                     /note="/other=CDS"
                     /translation="APNTQTASPRALADSLMQ"
     promoter        847..865
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     primer_bind     886..908
                     /label=pAPInt_R
     terminator      909..1106
                     /label=TEF terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     CDS             complement(1115..1921)
                     /codon_start=1
                     /label=G418
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWSRDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1115..1906)
                     /codon_start=1
                     /label=aphA1
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=98.5"
                     /note="/match_length=97.4"
                     /note="/other=CDS"
                     /translation="THVSRPRLNSNMDADLYGYKWSRDNVGQSGATIYRLYGKPDAPEL
                     FLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLE
                     EYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDER
                     NGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDL
                     AILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     misc_feature    1928..2045
                     /label=H2Ap
     misc_feature    2046..2262
                     /label=Shared promoter region
     misc_feature    2263..2306
                     /label=H2Bp (missing 22bp)
     primer_bind     complement(2307..2329)
                     /label=pAPDel_F
     terminator      2330..2517
                     /label=ADH1 terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     CDS             complement(2538..3245)
                     /codon_start=1
                     /label=mNeonGreen (codon optimized)
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     primer_bind     complement(3250..3269)
                     /label=pAPInt_F
     promoter        3315..3333
                     /label=SP6 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    3396..3447
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.9"
                     /note="/other=misc_feature"
     CDS             complement(3549..3584)
                     /codon_start=1
                     /label=rop (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=18.8"
                     /note="/other=CDS"
                     /translation="LARFGDDGENL"
     promoter        3679..3783
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    3784..4644
                     /label=AmpR
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaggttaa cctggcttat
      841 cgaaattaat acgactcact atagggagac cggcagatcc gcggccgcat aggccactag
      901 tggatctgca gtatagcgac cagcattcac atacgattga cgcatgatat tactttctgc
      961 gcacttaact tcgcatctgg gcagatgatg tcgaggcgaa aaaaaatata aatcacgcta
     1021 acatttgatt aaaatagaac aactacaata taaaaaaact atacaaatga caagttcttg
     1081 aaaacaagaa tctttttatt gtcagtactg actagaagaa ctcgtcgagc atgaggtgga
     1141 actggagctt gttcatgtcc gggttgtcga tgccgtactt ctggaagagg cgcttctgga
     1201 gcgacggcga gaactcgccg aggcagttcc agaggatggc gaggtcctgg tagcggtcgg
     1261 cgatgccgac gcggccgacg tcgatgcagc cgatgagctt gccctcgtcg aagatgaggt
     1321 tgtcgagcga gaagtcgccg tgcgtgacga ccgagtccgg cgagaacggg aggagcttgt
     1381 gcatctcctt ccagacctgc tcgaccggcc agccgttgcg ctcgtcgtcg aagtccgagg
     1441 cgtcgacgag gccgttgttc atgcgcgact gggcctgggc gaggcggaag acgcggtccg
     1501 agttgaacgg gcagttgcag accgggatcg agtggaggcg gcggaggaag acggcgaggg
     1561 cgtcgacgat gttctcgccc gagtccgggt actcctcgag gacctggaag gccgtcttgc
     1621 ccgggatggc cgtcgtgagg agccaggcgt cgtccggcgt gcggatgaag tgcttgatcg
     1681 tcgggagcgg catgaactcc gtgagccagt tgaggcggac catctcgtcc gtgacgtcgt
     1741 tggcgaccga gcccttgccg tgcttgagga agagctccgg ggcgtccggc ttgccgtaga
     1801 ggcggtagat cgtggcgccc gactggccga cgttgtcgcg ggaccacttg tagccgtaga
     1861 ggtcggcgtc catgttcgag ttgaggcgcg ggcgcgagac gtgcgtcttc tccttgccca
     1921 tgaattctgc tgtgtaaggt gtgcgtgaga gagagggtcg tgagtgagtc ttgaagagaa
     1981 acgtcaaagc ttgaagcgta gcagaatacg gaaaacgact tgggggaatg gcgaggagcg
     2041 tcgcctttta tgctccaagc ggagacgatg aggccgatat tacagtggct tagtagcgtg
     2101 tacaatattt ccataaaagg ctacatggtg agtttttcac gtgaccgtaa ttagtcccaa
     2161 ttaggcttta ttgtatattg ctactaagca tcgttaaatg acctgctcga agcttcgtct
     2221 ataaaaggcg ggcgtgtgtg cgcttccccc gctacgtctt tttcacaaac actttgcgac
     2281 ttgaccaaga cctttcaccc gcaccaccct gttatcccta gcggatctgc cggtagaggt
     2341 gtggtcaata agagcgacct catactatac ctgagaaagc aacctgacct acaggaaaga
     2401 gttactcaag aataagaatt ttcgttttaa aacctaagag tcactttaaa atttgtatac
     2461 acttattttt tttataactt atttaataat aaaaatcata aatcataaga aattcgctta
     2521 tttagaagtg gcgctcactt gtagagctcg tccatgccca tgacgtcggt gaaggccttt
     2581 tgccactcct tgaagttgag ctcggtctta gagtgcttga gctcggtctt gcggaagacg
     2641 tacataggtt ggttcttgag gtagttggcg gccataggct tggcgaaggt gtaggtggtg
     2701 cgggcggtag agcggtagcg cttgccgttg ccggtggtgt aagaccactt gaaggtagag
     2761 atgatggtct tgtcgttagg gtaggtcttc ttagagcggc accagtcggc ggcggtgaga
     2821 gagttggtca tgacagggcc gtcggcaggg aagccggtgc ccttgacttg ggcctcgccc
     2881 ttgatgtgag agccctcgta ggtgtatctg tagttgacag tgagagaggc gccgtcctcg
     2941 aattgcatgg tgcggtggac ttggtagcca gagccgtcga ccatggcggc ttggaaagga
     3001 gacatgccgt cagggtaagg gaggtattgg tggaagccgt agccgatgtg agggacgagg
     3061 atccaaggag agaattggag gtcgcccttg gtagacttga ggttgagctc ctcgtagccg
     3121 tcgttagggt tgccggtgcc ttggccgacc atgtcgaagt cgacgccgtt gatagagccg
     3181 aagatgtgga gctcgtgggt ggcagggaga gaggccatgt tgtcctcctc gcccttagag
     3241 accatagaac cagaaccagc accgtcaccg atccgtcgac ctgcagcgta cgaagcttca
     3301 gctggcggcc gcgttctata gtgtcaccta aatcgtatgt gtatgataca taaggttatg
     3361 tattaattgt agccgcgttc taacgacaat atgtccatat ggtgcactct cagtacaatc
     3421 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     3481 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     3541 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
     3601 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     3661 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     3721 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     3781 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     3841 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     3901 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     3961 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     4021 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     4081 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     4141 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     4201 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     4261 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     4321 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     4381 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     4441 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     4501 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     4561 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     4621 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     4681 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     4741 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     4801 atcaaaggat cttc
//
