LOCUS       Exported                4577 bp ds-DNA     circular SYN 11-MAR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 14, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     145..167
                     /label=pAPDel_F
     misc_feature    168..211
                     /label=H2Bp (missing 22bp)
     misc_feature    212..428
                     /label=Shared promoter region
     misc_feature    429..546
                     /label=H2Ap
     misc_feature    553..1125
                     /label=NatR
     terminator      1131..1328
                     /label=TEF terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     primer_bind     complement(1329..1351)
                     /label=pAPInt_R
     promoter        complement(1372..1390)
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             complement(1412..1465)
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=5.0"
                     /note="/other=CDS"
                     /translation="APNTQTASPRALADSLMQ"
     rep_origin      complement(1648..2236)
                     /direction=LEFT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(2407..3267)
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3268..3372)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             3467..3502
                     /codon_start=1
                     /label=rop (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=18.8"
                     /note="/other=CDS"
                     /translation="LARFGDDGENL"
     misc_feature    3604..3655
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.9"
                     /note="/other=misc_feature"
     promoter        3718..3736
                     /label=SP6 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     primer_bind     3782..3801
                     /label=pAPInt_F
     misc_feature    3806..4513
                     /label=mCherry (codon optimized)
     terminator      join(4534..4577,1..144)
                     /label=ADH1 terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
ORIGIN
        1 aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac gaaaattctt
       61 attcttgagt aactctttcc tgtaggtcag gttgctttct caggtatagt atgaggtcgc
      121 tcttattgac cacacctcta ccggcagatc cgctagggat aacagggtgg tgcgggtgaa
      181 aggtcttggt caagtcgcaa agtgtttgtg aaaaagacgt agcgggggaa gcgcacacac
      241 gcccgccttt tatagacgaa gcttcgagca ggtcatttaa cgatgcttag tagcaatata
      301 caataaagcc taattgggac taattacggt cacgtgaaaa actcaccatg tagcctttta
      361 tggaaatatt gtacacgcta ctaagccact gtaatatcgg cctcatcgtc tccgcttgga
      421 gcataaaagg cgacgctcct cgccattccc ccaagtcgtt ttccgtattc tgctacgctt
      481 caagctttga cgtttctctt caagactcac tcacgaccct ctctctcacg cacaccttac
      541 acagcagaat tcatgggtac cactcttgac gacacggctt accggtaccg caccagtgtc
      601 ccgggggacg ccgaggccat cgaggcactg gatgggtcct tcaccaccga caccgtcttc
      661 cgcgtcaccg ccaccgggga cggcttcacc ctgcgggagg tgccggtgga cccgcccctg
      721 accaaggtgt tccccgacga cgaatcggac gacgaatcgg acgacgggga ggacggcgac
      781 ccggactccc ggacgttcgt cgcgtacggg gacgacggcg acctggcggg cttcgtggtc
      841 gtctcgtact ccggctggaa ccgccggctg accgtcgagg acatcgaggt cgccccggag
      901 caccgggggc acggggtcgg gcgcgcgttg atggggctcg cgacggagtt cgcccgcgag
      961 cggggcgccg ggcacctctg gctggaggtc accaacgtca acgcaccggc gatccacgcg
     1021 taccggcgga tggggttcac cctctgcggc ctggacaccg ccctgtacga cggcaccgcc
     1081 tcggacggcg agcaggcgct ctacatgagc atgccctgcc cctaatcagt actgacaata
     1141 aaaagattct tgttttcaag aacttgtcat ttgtatagtt tttttatatt gtagttgttc
     1201 tattttaatc aaatgttagc gtgatttata ttttttttcg cctcgacatc atctgcccag
     1261 atgcgaagtt aagtgcgcag aaagtaatat catgcgtcaa tcgtatgtga atgctggtcg
     1321 ctatactgca gatccactag tggcctatgc ggccgcggat ctgccggtct ccctatagtg
     1381 agtcgtatta atttcgataa gccaggttaa cctgcattaa tgaatcggcc aacgcgcggg
     1441 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     1501 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     1561 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     1621 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     1681 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     1741 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     1801 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     1861 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     1921 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     1981 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     2041 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     2101 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     2161 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     2221 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     2281 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     2341 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     2401 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     2461 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     2521 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     2581 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     2641 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     2701 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     2761 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     2821 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     2881 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     2941 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     3001 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     3061 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     3121 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     3181 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     3241 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     3301 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     3361 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     3421 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
     3481 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     3541 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     3601 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatggacat
     3661 attgtcgtta gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt
     3721 taggtgacac tatagaacgc ggccgccagc tgaagcttcg tacgctgcag gtcgacggat
     3781 cggtgacggt gctggttctg gttctatggt ctctaagggc gaggaggaca acatggccat
     3841 catcaaggag ttcatgcgct tcaaggtcca catggagggc tctgtcaacg gccacgagtt
     3901 cgagatcgag ggcgagggcg agggccgccc ttacgagggc acccaaaccg ccaagctcaa
     3961 ggtcaccaag ggcggccctc tccctttcgc ctgggacatc ctctctcctc aattcatgta
     4021 cggctctaag gcctacgtca agcaccctgc cgacatccct gactacctca agctctcttt
     4081 ccctgagggc ttcaagtggg agcgcgtcat gaacttcgag gacggcggcg tcgtcaccgt
     4141 cacccaagac tcttctctcc aagacggcga gttcatctac aaggtcaagc tccgcggcac
     4201 caacttccct tctgacggcc ctgtcatgca aaagaagacc atgggctggg aggcctcttc
     4261 tgagcgcatg taccctgagg acggcgccct caagggcgag atcaagcaac gcctcaagct
     4321 caaggacggc ggccactacg acgccgaggt caagaccacc tacaaggcca agaagcctgt
     4381 ccaactccct ggcgcctaca acgtcaacat caagctcgac atcacctctc acaacgagga
     4441 ctacaccatc gtcgagcaat acgagcgcgc cgagggccgc cactctaccg gcggcatgga
     4501 cgagctctac aagtgagcgc cacttctaaa taagcgaatt tcttatgatt tatgattttt
     4561 attattaaat aagttat
//
