LOCUS       Exported                5034 bp ds-DNA     circular SYN 12-MAR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 14, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..5034
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     145..167
                     /label=pAPDel_F
     misc_feature    168..211
                     /label=H2Bp (missing 22bp)
     misc_feature    212..428
                     /label=Shared promoter region
     misc_feature    429..546
                     /label=H2Ap
     misc_feature    553..1581
                     /label=HygR aph(4)-la
     terminator      1587..1784
                     /label=TEF terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     primer_bind     complement(1785..1807)
                     /label=pAPInt_R
     promoter        complement(1828..1846)
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             complement(1868..1921)
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=5.0"
                     /note="/other=CDS"
                     /translation="APNTQTASPRALADSLMQ"
     rep_origin      complement(2104..2692)
                     /direction=LEFT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     misc_feature    complement(2863..3723)
                     /label=AmpR
     promoter        complement(3724..3828)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             3923..3958
                     /codon_start=1
                     /label=rop (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=18.8"
                     /note="/other=CDS"
                     /translation="LARFGDDGENL"
     misc_feature    4060..4111
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.9"
                     /note="/other=misc_feature"
     promoter        4174..4192
                     /label=SP6 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     primer_bind     4238..4257
                     /label=pAPInt_F
     CDS             4268..4963
                     /codon_start=1
                     /label=mScarlet (codon optimized)
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     misc_feature    join(4991..5034,1..144)
                     /label=ADH1 terminator
ORIGIN
        1 aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac gaaaattctt
       61 attcttgagt aactctttcc tgtaggtcag gttgctttct caggtatagt atgaggtcgc
      121 tcttattgac cacacctcta ccggcagatc cgctagggat aacagggtgg tgcgggtgaa
      181 aggtcttggt caagtcgcaa agtgtttgtg aaaaagacgt agcgggggaa gcgcacacac
      241 gcccgccttt tatagacgaa gcttcgagca ggtcatttaa cgatgcttag tagcaatata
      301 caataaagcc taattgggac taattacggt cacgtgaaaa actcaccatg tagcctttta
      361 tggaaatatt gtacacgcta ctaagccact gtaatatcgg cctcatcgtc tccgcttgga
      421 gcataaaagg cgacgctcct cgccattccc ccaagtcgtt ttccgtattc tgctacgctt
      481 caagctttga cgtttctctt caagactcac tcacgaccct ctctctcacg cacaccttac
      541 acagcagaat tcatgggtaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg
      601 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt
      661 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
      721 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg
      781 gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca
      841 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc
      901 gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca
      961 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
     1021 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg
     1081 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
     1141 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt
     1201 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
     1261 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
     1321 cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat
     1381 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca
     1441 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
     1501 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
     1561 cgtccgaggg caaaggaata atcagtactg acaataaaaa gattcttgtt ttcaagaact
     1621 tgtcatttgt atagtttttt tatattgtag ttgttctatt ttaatcaaat gttagcgtga
     1681 tttatatttt ttttcgcctc gacatcatct gcccagatgc gaagttaagt gcgcagaaag
     1741 taatatcatg cgtcaatcgt atgtgaatgc tggtcgctat actgcagatc cactagtggc
     1801 ctatgcggcc gcggatctgc cggtctccct atagtgagtc gtattaattt cgataagcca
     1861 ggttaacctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     1921 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     1981 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     2041 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     2101 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     2161 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     2221 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     2281 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     2341 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     2401 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     2461 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     2521 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     2581 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     2641 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     2701 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     2761 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     2821 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     2881 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     2941 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     3001 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     3061 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     3121 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     3181 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     3241 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     3301 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     3361 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     3421 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     3481 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     3541 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     3601 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     3661 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     3721 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     3781 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     3841 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     3901 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     3961 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     4021 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     4081 agagcagatt gtactgagag tgcaccatat ggacatattg tcgttagaac gcggctacaa
     4141 ttaatacata accttatgta tcatacacat acgatttagg tgacactata gaacgcggcc
     4201 gccagctgaa gcttcgtacg ctgcaggtcg acggatcggt gacggtgctg gttctggttc
     4261 tggtaccatg gtctctaagg gcgaggccgt catcaaggag ttcatgcgct tcaaggtcca
     4321 catggagggc tctatgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     4381 ttacgagggc acccaaaccg ccaagctcaa ggtcaccaag ggcggccctc tccctttctc
     4441 ttgggacatc ctctctcctc aattcatgta cggctctcgc gccttcacca agcaccctgc
     4501 cgacatccct gactactaca agcaatcttt ccctgagggc ttcaagtggg agcgcgtcat
     4561 gaacttcgag gacggcggcg ccgtcaccgt cacccaagac acctctctcg aggacggcac
     4621 cctcatctac aaggtcaagc tccgcggcac caacttccct cctgacggcc ctgtcatgca
     4681 aaagaagaca atgggctggg aggcctctac cgagcgcctc taccctgagg acggcgtcct
     4741 caagggcgac atcaagatgg ccctccgcct caaggacggc ggccgctacc tcgccgactt
     4801 caagaccacc tacaaggcca agaagcctgt ccaaatgcct ggcgcctaca acgtcgaccg
     4861 caagctcgac atcacctctc acaacgagga ctacaccgtc gtcgagcaat acgagcgctc
     4921 tgagggccgc cactctaccg gcggcatgga cgagctctac aagtgaatct agagcgccac
     4981 ttctaaataa gcgaatttct tatgatttat gatttttatt attaaataag ttat
//
