LOCUS       Exported                5015 bp ds-DNA     circular SYN 12-MAR-2025
DEFINITION  DLB4906.
ACCESSION   DLB4906
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 14, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             772..825
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=5.0"
                     /note="/other=CDS"
                     /translation="APNTQTASPRALADSLMQ"
     promoter        847..865
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     primer_bind     886..908
                     /label=pAPInt_R
     terminator      909..1106
                     /label=TEF terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     misc_feature    complement(1112..2140)
                     /label=HygR aph(4)-la
     misc_feature    2147..2264
                     /label=H2Ap
     misc_feature    2265..2481
                     /label=Shared promoter region
     misc_feature    2482..2525
                     /label=H2Bp (missing 22bp)
     primer_bind     complement(2526..2548)
                     /label=pAPDel_F
     terminator      2549..2736
                     /label=ADH1 terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     CDS             complement(2754..3446)
                     /codon_start=1
                     /label=Dendra2 (codon optimized)
                     /label=Dendra2 - Ap optimized (100%)
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
     primer_bind     complement(3451..3470)
                     /label=pAPInt_F
     promoter        3516..3534
                     /label=SP6 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    3597..3648
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.9"
                     /note="/other=misc_feature"
     CDS             complement(3750..3785)
                     /codon_start=1
                     /label=rop (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=18.8"
                     /note="/other=CDS"
                     /translation="LARFGDDGENL"
     promoter        3880..3984
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    3985..4845
                     /label=AmpR
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaggttaa cctggcttat
      841 cgaaattaat acgactcact atagggagac cggcagatcc gcggccgcat aggccactag
      901 tggatctgca gtatagcgac cagcattcac atacgattga cgcatgatat tactttctgc
      961 gcacttaact tcgcatctgg gcagatgatg tcgaggcgaa aaaaaatata aatcacgcta
     1021 acatttgatt aaaatagaac aactacaata taaaaaaact atacaaatga caagttcttg
     1081 aaaacaagaa tctttttatt gtcagtactg attattcctt tgccctcgga cgagtgctgg
     1141 ggcgtcggtt tccactatcg gcgagtactt ctacacagcc atcggtccag acggccgcgc
     1201 ttctgcgggc gatttgtgta cgcccgacag tcccggctcc ggatcggacg attgcgtcgc
     1261 atcgaccctg cgcccaagct gcatcatcga aattgccgtc aaccaagctc tgatagagtt
     1321 ggtcaagacc aatgcggagc atatacgccc ggagccgcgg cgatcctgca agctccggat
     1381 gcctccgctc gaagtagcgc gtctgctgct ccatacaagc caaccacggc ctccagaaga
     1441 agatgttggc gacctcgtat tgggaatccc cgaacatcgc ctcgctccag tcaatgaccg
     1501 ctgttatgcg gccattgtcc gtcaggacat tgttggagcc gaaatccgcg tgcacgaggt
     1561 gccggacttc ggggcagtcc tcggcccaaa gcatcagctc atcgagagcc tgcgcgacgg
     1621 acgcactgac ggtgtcgtcc atcacagttt gccagtgata cacatgggga tcagcaatcg
     1681 cgcatatgaa atcacgccat gtagtgtatt gaccgattcc ttgcggtccg aatgggccga
     1741 acccgctcgt ctggctaaga tcggccgcag cgatcgcatc catggcctcc gcgaccggct
     1801 gcagaacagc gggcagttcg gtttcaggca ggtcttgcaa cgtgacaccc tgtgcacggc
     1861 gggagatgca ataggtcagg ctctcgctga attccccaat gtcaagcact tccggaatcg
     1921 ggagcgcggc cgatgcaaag tgccgataaa cataacgatc tttgtagaaa ccatcggcgc
     1981 agctatttac ccgcaggaca tatccacgcc ctcctacatc gaagctgaaa gcacgagatt
     2041 cttcgccctc cgagagctgc atcaggtcgg agacgctgtc gaacttttcg atcagaaact
     2101 tctcgacaga cgtcgcggtg agttcaggct ttttacccat gaattctgct gtgtaaggtg
     2161 tgcgtgagag agagggtcgt gagtgagtct tgaagagaaa cgtcaaagct tgaagcgtag
     2221 cagaatacgg aaaacgactt gggggaatgg cgaggagcgt cgccttttat gctccaagcg
     2281 gagacgatga ggccgatatt acagtggctt agtagcgtgt acaatatttc cataaaaggc
     2341 tacatggtga gtttttcacg tgaccgtaat tagtcccaat taggctttat tgtatattgc
     2401 tactaagcat cgttaaatga cctgctcgaa gcttcgtcta taaaaggcgg gcgtgtgtgc
     2461 gcttcccccg ctacgtcttt ttcacaaaca ctttgcgact tgaccaagac ctttcacccg
     2521 caccaccctg ttatccctag cggatctgcc ggtagaggtg tggtcaataa gagcgacctc
     2581 atactatacc tgagaaagca acctgaccta caggaaagag ttactcaaga ataagaattt
     2641 tcgttttaaa acctaagagt cactttaaaa tttgtataca cttatttttt ttataactta
     2701 tttaataata aaaatcataa atcataagaa attcgcttat ttagaagtgg cgctcaccag
     2761 acttgagaag ggagaggaga gtagcgggcg acggcgtgct cgtagagctt gaccttgttg
     2821 tagtcagagt cgttgccgag gatctcgatg cggtggtcga cgaagtgggc gtcagggagt
     2881 tggacgacct tcttggcctt gtaggtggtc ttgaagtcgc agaggtagtg gccgccgccc
     2941 tcgaggagga gggccatgtt gatgttgccg acgaggaggc cgtcgcggac gtggagcttc
     3001 tcggtagaag gctcccactt gagggtcttc ttttgcatga cagggccgtt aggagggaag
     3061 ttggtgccct tgaagcggac gttttggaag aagcagtcgc cctcgagaga gatgtcagag
     3121 cggatggtgc agatgccctt gtcctcgaag gtcatggtgc gctcccaaga gtagccctca
     3181 gggaaagatt gcttgaagta gtcagggatg tcctcagggt acttggtgaa gacgcggttg
     3241 ccgtagtgga cggcggtggt gaggatgtcg taagagaaag ggagaggggc gccctccttg
     3301 acggtgaggt tggcggtttg ggtgccctcg taaggcttgc ccttgccctc gccctcgatg
     3361 acgaaggcgt ggccgttgac gttgccctcc atgtggacct tgacgcgcat gtcctccttg
     3421 atgaggttga tgccaggggt gttcatagaa ccagaaccag caccgtcacc gatccgtcga
     3481 cctgcagcgt acgaagcttc agctggcggc cgcgttctat agtgtcacct aaatcgtatg
     3541 tgtatgatac ataaggttat gtattaattg tagccgcgtt ctaacgacaa tatgtccata
     3601 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     3661 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     3721 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     3781 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     3841 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     3901 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     3961 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     4021 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     4081 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     4141 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     4201 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     4261 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     4321 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     4381 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     4441 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     4501 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     4561 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     4621 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     4681 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     4741 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     4801 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     4861 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     4921 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     4981 gcgtcagacc ccgtagaaaa gatcaaagga tcttc
//
