LOCUS       Exported               11409 bp ds-DNA     circular SYN 28-FEB-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 14, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.1
FEATURES             Location/Qualifiers
     source          1..11409
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
     CDS             772..864
                     /label=lacI (fragment)
                     /note="pLannotate"
     protein_bind    877..898
                     /label=CAP binding site
                     /note="pLannotate"
     promoter        913..943
                     /label=lac promoter pLannotate
                     /label=lac promoter
                     /note="pLannotate"
     protein_bind    947..969
                     /label=lac operator (fragment)
                     /note="pLannotate"
     polyA_signal    complement(1040..1161)
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
     CDS             complement(1156..1314)
                     /label=ble (fragment)
                     /note="pLannotate"
     CDS             complement(1338..2129)
                     /label=neo
                     /note="pLannotate"
     promoter        complement(2196..2525)
                     /label=SV40 promoter pLannotate
                     /label=SV40 promoter
                     /note="pLannotate"
     rep_origin      complement(2539..2967)
                     /direction=LEFT
                     /label=f1 ori
                     /note="pLannotate"
     promoter        complement(2988..3009)
                     /label=SV40 promoter (fragment) pLannotate
                     /label=SV40 promoter (fragment)
                     /note="pLannotate"
     polyA_signal    complement(3013..3237)
                     /label=bGH poly(A) signal
                     /note="pLannotate"
     CDS             complement(3287..3304)
                     /label=capTEV(TM) (fragment)
                     /note="pLannotate"
     misc_feature    3320..3349
                     /label=c-Myc Tag
     CDS             complement(3368..8578)
                     /label=mouse_ZO-1(TJP1)_FullLenght
                     /label=Tjp1
                     /note="pLannotate"
     CDS             complement(8588..9304)
                     /label=EGFP
                     /note="pLannotate"
     misc_feature    complement(9311..9329)
                     /label=MCS (fragment)
                     /note="pLannotate"
     promoter        complement(9362..9380)
                     /label=T7 promoter pLannotate
                     /label=T7 promoter
                     /note="pLannotate"
     LTR             complement(9396..9429)
                     /label=5' LTR (truncated) (fragment)
                     /note="pLannotate"
     promoter        complement(9425..9628)
                     /label=CMV promoter pLannotate
                     /label=CMV promoter
                     /note="pLannotate"
     enhancer        complement(9629..10008)
                     /label=CMV enhancer pLannotate
                     /label=CMV enhancer
                     /note="pLannotate"
     misc_feature    10163..10215
                     /label=bom (fragment)
                     /note="pLannotate"
     promoter        10274..10378
                     /label=AmpR promoter pLannotate
                     /label=AmpR promoter
                     /note="pLannotate"
     CDS             10379..11239
                     /label=AmpR
                     /note="pLannotate"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1081 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1141 ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg
     1201 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1261 ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa
     1321 ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
     1381 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
     1441 tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg
     1501 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
     1561 tcgccatggg tcacgacgag atcctcgccg tcgggcatgc gcgccttgag cctggcgaac
     1621 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
     1681 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
     1741 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
     1801 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
     1861 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
     1921 agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga caggtcggtc
     1981 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
     2041 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
     2101 cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca
     2161 gatccgaaaa tggatataca agctcccggg agctttttgc aaaagcctag gcctccaaaa
     2221 aagcctcctc actacttctg gaatagctca gaggcagagg cggcctcggc ctctgcataa
     2281 ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc
     2341 gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc
     2401 atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga
     2461 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga
     2521 cacacattcc acagaattaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     2581 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     2641 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     2701 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     2761 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     2821 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     2881 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     2941 cgcttaatgc gccgctacag ggcgcgtggg gataccccct agagccccag ctggttcttt
     3001 ccgcctcaga agccatagag cccaccgcat ccccagcatg cctgctattg tcttcccaat
     3061 cctccccctt gctgtcctgc cccaccccac cccccagaat agaatgacac ctactcagac
     3121 aatgcgatgc aatttcctca ttttattagg aaaggacagt gggagtggca ccttccaggg
     3181 tcaaggaagg cacgggggag gggcaaacaa cagatggctg gcaactagaa ggcacagtcg
     3241 aggctgatca gcggtttaaa cttaagctgg agaccgttta aactcaatga tgatgatgat
     3301 gatggtcgac ggcgctattc agatcctctt ctgagatgag tttttgttcg ggcccaagct
     3361 tggtaccaaa gtggtcaatc aggacagaaa cacagttggc tccaacaagg taattcggat
     3421 ctccaggaag acacttgttt tgccaggttt tagggtcaca gtgtggcaag cgtagctcca
     3481 cgggcttcag gaacttgagg ccatggggcc cacacatcac tagggggctc agcagagttt
     3541 cacctttctc tttatctaaa ggtgggagaa tgctattgtc tctgcagact ttgaaataga
     3601 tttcttgctc aattccttca ggaatggctc cttgtgggat aattatacta acaccggttt
     3661 ctatggaact caacacacca ccattgctgt taaaaatgcc acgagctgta gccactacag
     3721 tatgaccatc ctcatcttca tcttcttcca cagctgaagg actcacaggg acagctttag
     3781 gcatggtgct gatattattc atttgatatt taggcttatc tgtgtgaaca gagaaagttt
     3841 caactccact gccaaactct ggaggctgtg ttgaactatg agctttcata agagtttttg
     3901 gggaagtggg agtttttgaa gacaattcag gtttatgtac agtttcactt ggcagaagat
     3961 tatggttgaa cttcggactt tcaaatttgc gttcaaatgg ccgggcagaa cttgtgtacg
     4021 gctttggtgt gaatcgattg tacaccggag tgatggtttt ctgagtctgc tcagctccgt
     4081 taactggagc tttgtctggg aaacttttct gcggatagaa agtctgaggg gggtcttctc
     4141 gagttggtgg tctgaaagtt gctggtttac tctgagatgg gggtgggtct ggtttggaca
     4201 tatatgagtt ctgaaaatcc aatgatactg agttgccttc accctgggcg cccttggaat
     4261 gtatgtggag agaagagctg gacagaggtg gagctggctg gctgtactgt gagggcaacg
     4321 gaggaggagg aggcgtggcc tgggctggat cataacgttt ctcactgaat gatctatcca
     4381 cagcatcagt ttcgggtttt ccctttgaag agtaactagg gaagttgggc tggctctgag
     4441 aatggactgg cttagcaggc tctgagtgat ggccagcagg aagatgtgca gaaggcttgc
     4501 tctcaaaact tcttcggtca aagtaggaga gctgtttccg gtaatattct tcatcctctt
     4561 cagggtcgta atgatttgat cgaacaatat cttcgggtgg cttcacttga ggtttctgag
     4621 gctctgggag cctgtagagc gttttgtcgt gctcactaaa ctgactctga gggacaggtt
     4681 tagggccagc aagagaagca cctggaggtt tagatgctac ttccggaggc ttgaagctgg
     4741 cagtgtcatt cacatctttc ttgttctcca aagacgcaga tcttttgttt tcaaacattt
     4801 tgactctggt gagcacagac tgtggtttca ttgctggatc ctcctcctcc tcagttaggg
     4861 ttgggggtgg aggctgtggt ttggtagccg agggcaggac ttctggcttt tgttgagagg
     4921 aaggtatcag aggagggaca actgcagcac catggagagg ctcgtaatgg cctgttttgg
     4981 aggtaaagcc tggtggtgga acttgctcat aacttcgcgg gtactggtca aagtactgct
     5041 tgggctcaga tgacttaggg cctgttgaag aatagggctg tgcctctggc ctgtacctgt
     5101 tgtgcacctc atatgcaggg gctggctgct cttcatgtcg tagagtagag gttcgaggca
     5161 gctgctcata gcgtgttcta ctgtcgtacg acagaggggc tggctcttca aaacgctgga
     5221 aataacctcg ttctgaagcc tcttcgggat gttgtctgga gtccaggtct cggggatgct
     5281 gattctcaaa aggccgaggt tggtagggct gtttgtcatc ataatatgac cactggtctt
     5341 cataggtagg gattcgatct tcaaaccgta ggcgatggtc atagttccga gaaaactggt
     5401 ctgtgtagct tgagacctca tacctgtatg acggctgctc aaggtctctg ctggcttgtt
     5461 tttctatata tggaagttgg ggttcatagg ctagatttgg ctctttatct ggcctctgcc
     5521 ctgggtgacc aagagctggt tgttttaaaa tatggttttg tctcatcatt tcttcagaat
     5581 acggctcctt cctgtacacc tttgctgggt ctgcatgcgg cggcaatggt ggtccttcac
     5641 ctctgagcac tacgtgagct gcctcagcac ttggtgctcc taaacaacca ggctgtgatg
     5701 cgtgcgaggt gggaggggct ggggcaggct cctccaggct gacattagtt acactgacat
     5761 tatgattaac tgcagaggct gatgatgctg gggttgtttc aggcgaaagg taagggactg
     5821 gagatgaggc ttctgctttc tgttgagagg ctggcttaag tccaggggag tctattctat
     5881 gaatagcttg tggctgcgct tgtggtgagt aaggagggta tgtctggttt tcatgatgca
     5941 ttccagagga atcctctctt acaggctcag aggaccgtgt aatggcagac tccgggggag
     6001 tccccacctc atcattaaga gtttcatcta gttcttgatc agtgtaggcc ccgccttctg
     6061 tatctgtgtc ttcatagtca gaagtgtgtc tactgtccgt gctatacatt gagtactcac
     6121 tacctggggc tgacaggtag gacagacgat cgtcatgcaa atcaaggtca tcacttgtag
     6181 caccatccgc cttcccctca gagacccaca ccagctggtt ctgttgctgc tgaatcgctt
     6241 ctttcagggc accgtaccaa ccatcattca ttgagtttaa gttaattgta gttgtgaaga
     6301 gatggtgatt gttcttacga agcttatgag agcgttcata tagcttcctg gcgcttttcc
     6361 gagactccgg acacaacctc atcctcattg tttttacacc ttgcttagag tcagggttaa
     6421 ggaacacaac aattggatac cactgcgcat aattaagacg atcaaccgca tttggcgtta
     6481 catctaataa agcatgttta tcctgatcta tgatttgctt tattgtatga aggcgaatga
     6541 tgccagagct acgatggtca gtcccagcat ctcgtggttc actttttgca atctgataga
     6601 tatctggctc ctctcttgcc aacttttctc tggcaacatc agctattggt ccaaagatgg
     6661 ttacaggtct taggaatcca gcttctcgaa gaacaaccct ttcataagct gggaacttag
     6721 tttgaactgg ctgagctgac aagtcctctc tgctttttcg aagatttctc ttggagctgc
     6781 gaagacctcg aaacctccag aagtctgccc gatcaccacc cgctgtcttt ggaagtgtgt
     6841 actgtacact ggctaactgt tcagctctgt tcttattagg gatgatgcct cgttctacct
     6901 ccttatgatt tttgccaatt cgaatggcaa gccaagagcc cagctttcca ttgtataaag
     6961 tatccacgac ccggaacacc tctcctttgt taaaactaag tccgtaagga gattcttttt
     7021 catattcaaa atgcgttcta atatagaatg aatctcctac atctgattct acaatgcggc
     7081 gataaacgtc cttcttcttc tgagccagta tggtcacttc ttcaccttta gggaggtcaa
     7141 ggaggaaaag gacggcctct tcccttatga tatttgtgaa atctacattg ttcaccctga
     7201 gaatttgatc accttcctct aagccttctt tggctgcagg gctatcttct agaacgccag
     7261 ccacaaatat tccgacatca tttccaccag ctagtcgcaa acccacacta tctccttttc
     7321 tgaatttcac cagtttcatg ctgggcctaa gtatcccgtc ttcatgagct gaattaggca
     7381 gagcaccatc agaagggctg acgggtaaat ccacatctgg ttgtccaact tgagcataca
     7441 caggtttcgg ttctggaagg gtgggcgtct gcttctcatt tttctcaact gtgacttctt
     7501 ccactgcttt gggtggatga tcgtctacat gttttacagg agttgagatg gccccaggtt
     7561 tagacattcg ctcttcctct ctgctccgga gactgccatt gctgggctgc tgtggagact
     7621 gcgtggaatg atcggagggc tctgaacgtt ggtcaggaga tcgtgactgg ctgcggcggg
     7681 gtggcctgtc atgcgagcga cctgaatggt ctgatgctag tgactgaatt tctgaaatgt
     7741 catctctttc cgaggcatta gcagaatgga tactatccga aaggtcaggg acgttcagta
     7801 aggtagcccg ctcatctctt tgcactacca tttttaactt gcctttagac ctttctatca
     7861 gtgtttttgc atctgtcaat gacatatttt ctgtcacagt accatttatc ttcaagacaa
     7921 catccccttc ttgaatgtta ccatctcttg ctgccaaact atcttgtgaa atttccttta
     7981 caaatatatg gctggccaat cgaagaccat attcttcatt tttccgagac ttcaccagtg
     8041 cgaccttggt gggctttgcg ggctgactgg aggccacgga ccgcctgtcc gagcgagggg
     8101 acaggctcct ctccctgctt gcactcctat cccttgccca gctcttctcg ctccttctgt
     8161 tcgctaaagc accgcggcca gctcttgggt catgcacttc ttcgtcataa ctatcgtctt
     8221 cattatcaga caccggctca ggatctgggt gacttacagg gatctgaact ttcttcttcc
     8281 ttcggatagt aatttttgcg tttttcccac tcttccttag ctgctgaaca gcaaaagcat
     8341 gttcaacgtt atccattgaa actccgttaa ccattgcaac tcggtcattt tcctgtagct
     8401 gtccttcagc tggccctcct tttaacacat cagaaatcac tatggaggtt tccccaccct
     8461 gaaaatgagg attatctctt ccaccagaga ttgcaattcc aaatccaaac ccaggagccc
     8521 tgtgaagcgt cactgtgtgc tgttcccata tagctgtttc ctccattgct gtgctcttgc
     8581 ggccgctctt gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca
     8641 gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac tgggtgctca
     8701 ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt
     8761 ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga
     8821 tgccgttctt ctgcttgtcg gccatgatat agacgttgtg gctgttgtag ttgtactcca
     8881 gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt
     8941 tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct
     9001 tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt
     9061 gctgcttcat gtggtcgggg tagcggctga agcactgcac gccgtaggtc agggtggtca
     9121 cgagggtggg ccagggcacg ggcagcttgc cggtggtgca gatgaacttc agggtcagct
     9181 tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt
     9241 cgccgtccag ctcgaccagg atgggcacca ccccggtgaa cagctcctcg cccttgctca
     9301 ccatggtggc ggatccacta gttctagagg gcccgtttaa acgctagcca gcttgggtct
     9361 ccctatagtg agtcgtatta atttcgataa gccagtaagc agtgggttct ctagttagcc
     9421 agagagctct gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg
     9481 gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc
     9541 cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc
     9601 cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt
     9661 actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt
     9721 taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca
     9781 agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg
     9841 ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc
     9901 caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta
     9961 atgaccccgt aattgattac tattaataac tagtcaataa tcaatgtcaa cgcgtatatc
    10021 tggcccgtac atcgcgaagc agcgcaaaac gcctaaccct aagcagattc ttcatgcaat
    10081 tgtcggtcaa gccttgcctt gttgtagctt aaattttgct cgcgcactac tcagcgacct
    10141 ccaacacaca agcagggagc agatactggc ttaactatgc ggcatcagag cagattgtac
    10201 tgagagtgca ccatagggga tcgggagatc tcccgatccg tcgacgtcag gtggcacttt
    10261 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
    10321 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
    10381 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
    10441 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
    10501 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
    10561 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
    10621 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
    10681 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
    10741 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
    10801 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
    10861 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
    10921 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
    10981 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
    11041 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
    11101 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
    11161 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
    11221 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
    11281 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
    11341 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
    11401 aggatcttc
//
