LOCUS       pTW1319_pMK003_ISDra2_ 14713 bp ds-DNA     circular     24-JUL-2023
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name: ptw1260_11861239_nt
            v_isdra2wrna_single_transcript_adding_back_7ntccdbptrans_230d.gb
FEATURES             Location/Qualifiers
     promoter        315..992
                     /label="35S promoter"
     gene            1060..2085
                     /label="HYG R"
     misc_feature    2377..2401
                     /label="LB"
                     /feature_type="Misc."
     gene            2826..3620
                     /label="KAN R"
     misc_feature    8656..8680
                     /label="RB"
                     /feature_type="Misc."
     misc_feature    8878..8898
                     /label="21401_Zheng"
                     /note="sequence: acgttgtaaaacgacggccag"
     misc_feature    complement(8882..8899)
                     /label="Reverse_primer_TnpB_gRNA_cloningjmhk,jk.jl.kl/.kl/k;/l"
                     /note="sequence: tgttcaacgaagcttctagtgactggccgtcgttttaca"
     misc_feature    complement(8889..8903)
                     /label="Backbone-Term_F"
                     /note="sequence: agagctctggtgacagtgagtgtggcactggccgtcg"
     primer_bind     8932..8984
                     /label=20544
                     /_Identity=100
                     /Motif="ATCACTAGTATCCTAGGAAGGTACCAGTCTAGCTCAACAGAGCTTTTAACCCA"
                     /Sequence="ATCACTAGTATCCTAGGAAGGTACCAGTCTAGCTCAACAGAGCTTTTAACCCA"
                     /Tm="67.2"
                     /GC="43.4"
                     /Hairpin_Tm="44.4"
                     /Self_Dimer_Tm="18.8"
                     /modified_by="cat"
     misc_feature    8935..8940
                     /label="SpeI site"
                     /feature_type="Misc."
     misc_feature    8935..8940
                     /label="SpeI"
                     /Mismatches=0
                     /_Identity=100
                     /Motif="ACTAGT"
                     /annotation_group="SpeI: 5,618 <- 5,623"
     misc_feature    complement(8935..8940)
                     /label="SpeI"
                     /Mismatches=0
                     /_Identity=100
                     /Motif="ACTAGT"
                     /annotation_group="SpeI: 5,618 -> 5,623"
     misc_feature    8955..8982
                     /label="Prom_F"
                     /note="sequence: tcggcgcgccagtctagctcaacagagcttttaacc"
     source          8957..10942
                     /label="source"
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    8957..10942
                     /label="pUBQ10"
     misc_feature    complement(9034..9058)
                     /label="Jason_20529"
                     /note="sequence: tggcagatagaatagctaaggaatc"
     misc_feature    10086..10130
                     /label=22381
                     /Mismatches=0
                     /_Identity=100
                     /Motif="AAATGGCAAAAAAAAACAAAAAGGAAAAGAAATAAAGCACGAAGA"
                     /annotation_group="22381: 10,086 -> 10,130"
     misc_feature    10086..11044
                     /label="959 bp fragment"
     misc_feature    10129..10134
                     /label="EcoRI"
                     /Mismatches=0
                     /_Identity=100
                     /Motif="GAATTC"
                     /annotation_group="EcoRI: 10,129 -> 10,134"
     misc_feature    complement(10129..10134)
                     /label="EcoRI"
                     /Mismatches=0
                     /_Identity=100
                     /Motif="GAATTC"
                     /annotation_group="EcoRI: 10,129 <- 10,134"
     primer_bind     10500..10519
                     /label=18617
     promoter        10639..10942
                     /label="UBQ10 intron"
     misc_feature    complement(10907..10942)
                     /label="Prom_R."
                     /note="sequence:gcacaaaagtgcctgcaggctgttaatcagaaaaactcagattaatcgacaaatt"
     misc_feature    complement(10908..10942)
                     /label="Prom_R.."
                     /note="sequence:gcacaaaagtacctgcaggctgttaatcagaaaaactcagattaatcgacaaat"
     misc_feature    complement(10908..10942)
                     /label="Prom_R"
                     /note="sequence:gcacaaaagtacctgcaggctgttaatcagaaaaactcagattaatcgacaaat"
     CDS             10943..10945
                     /label="ATG"
                     /product="<html><body>start codon</body></html>"
                     /translation="M"
     misc_feature    10943..11044
                     /label="misc"
                     /note="Geneious type: Editing History Insertion"
                     /Original_Bases=""
     CDS             10946..10993
                     /label="2xFLAG"
                     /product="<html><body>two tandem FLAG®epitopetags</body></html>"
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             11000..11020
                     /label="SV40 NLS"
                     /note="<html><body><br></body></html>"
                     /locus_tag="<html><body><br></body></html>"
                     /product="<html><body>nuclear localization signal ofSV40large T antigen</body></html>"
                     /protein_id="<html><body><br></body></html>"
                     /translation="PKKKRKV"
     primer          11015..11071
                     /label="ISDra2_ntv_fwd"
                     /note="sequence: aaggttggaatccacggagttccagctgctATGATAAGGAATAAGGCTTTCGTGGTC"
     misc_feature    11015..12087
                     /label="1063 bp fragment"
     primer_bind     11025..11044
                     /label=26572
                     /Sequence="TCCACGGAGTTCCAGCTGCTATGTCGCAGACCATCACAGTCA"
                     /Tm="70.7"
                     /GC="54.8"
                     /Hairpin_Tm="47.2"
                     /Self_Dimer_Tm="12.7"
                     /created_by="cat"
     primer_bind     complement(11025..11044)
                     /label=20478
                     /GC="60.0"
                     /Hairpin_Tm="39.4"
                     /Self_Dimer_Tm="17.1"
                     /Tm="63.6"
                     /annotation_group="180399230532100409b548d-e1c4-410f-96c6-fed59a00bb7d"
                     /created_by="primer3"
                     /Sequence="AGCAGCTGGAACTCCGTGGA"
                     /Mismatches=0
     misc_feature    11045..11046
                     /label="2>3"
     primer          11045..11071
                     /label="ISDra2_ntv_fwd_check"
                     /note="sequence: ATGATAAGGAATAAGGCTTTCGTGGTC"
     misc_feature    11045..12271
                     /label="IDT synthesis"
                     /created_by="cat"
     CDS             11045..12271
                     /label="ISDra2 TnpB Native"
                     /translation="MIRNKAFVVRLYPNAAQTELINRTLGSARFVYNHFLARRIAAYKESGKGLTYGQTSSELTLLKQAEETSWLSEVDKFALQNSLKNLETAYKNFFRTVKQSGKKVGFPRFRKKRTGESYRTQFTNNNIQIGEGRLKLPKLGWVKTKGQQDIQGKILNVTVRRIHEGHYEASVLCEVEIPYLPAAPKFAAGVDVGIKDFAIVTDGVRFKHEQNPKYYRSTLKRLRKAQQTLSRRKKGSARYGKAKTKLARIHKRIVNKRQDFLHKLTTSLVREYEIIGTEHLKPDNMRKNRRLALSISDAGWGEFIRQLEYKAAWYGRLVSKVSPYFPSSQLCHDCGFKNPEVKNLAVRTWTCPNCGETHDRDENAALNIRREALVAAGISDTLNAHGGYVRPASAGNGLRSENHATLVV*"
     CDS             11045..12271
                     /label="ISDra2 TnpB (TTGAT TAM)"
                     /note="codon_start: 1 transl_table: 1 translation: MIRNKAFVVRLYPNAAQTELINRTLGSARFVYNHFLARRIAAYKESGKGLTYGQTSSELTLLKQAEETSWLSEVDKFALQNSLKNLETAYKNFFRTVKQSGKKVGFPRFRKKRTGESYRTQFTNNNIQIGEGRLKLPKLGWVKTKGQQDIQGKILNVTVRRIHEGHYEASVLCEVEIPYLPAAPKFAAGVDVGIKDFAIVTDGVRFKHEQNPKYYRSTLKRLRKAQQTLSRRKKGSARYGKAKTKLARIHKRIVNKRQDFLHKLTTSLVREYEIIGTEHLKPDNMRKNRRLALSISDAGWGEFIRQLEYKAAWYGRLVSKVSPYFPSSQLCHDCGFKNPEVKNLAVRTWTCPNCGETHDRDENAALNIRREALVAAGISDTLNAHGGYVRPASAGNGLRSENHATLVV*"
                     /translation="MIRNKAFVVRLYPNAAQTELINRTLGSARFVYNHFLARRIAAYKESGKGLTYGQTSSELTLLKQAEETSWLSEVDKFALQNSLKNLETAYKNFFRTVKQSGKKVGFPRFRKKRTGESYRTQFTNNNIQIGEGRLKLPKLGWVKTKGQQDIQGKILNVTVRRIHEGHYEASVLCEVEIPYLPAAPKFAAGVDVGIKDFAIVTDGVRFKHEQNPKYYRSTLKRLRKAQQTLSRRKKGSARYGKAKTKLARIHKRIVNKRQDFLHKLTTSLVREYEIIGTEHLKPDNMRKNRRLALSISDAGWGEFIRQLEYKAAWYGRLVSKVSPYFPSSQLCHDCGFKNPEVKNLAVRTWTCPNCGETHDRDENAALNIRREALVAAGISDTLNAHGGYVRPASAGNGLRSENHATLVV*"
     misc_feature    11305..11305
                     /label="silent G>A"
     primer          12048..12080
                     /label="ISDra2_scaffold_fwd"
                     /note="sequence: gattcaagaatcccgaagtgaagaatcttgccg"
     misc_feature    12048..12271
                     /label="ISDra2 scaffold"
     primer          complement(12066..12087)
                     /label="ISDra2_ntv_rev_2"
                     /note="sequence: gtacggacggcaagattcttca"
     misc_feature    12127..12127
                     /label="was a T in pJA006. should be a C"
     primer          complement(12252..12271)
                     /label="ISDra2_ntv_rev_check"
                     /note="sequence: tcacacgactaaagtcgcgt"
     primer          complement(12252..12301)
                     /label="ISDra2_scaffold_rev_adding_ccdb"
                     /note="sequence: gtacaaacttgtcacctgcgatgTTGAACCtcacacgactaaagtcgcgt"
     misc_feature    12272..12278
                     /label="reRNA"
     misc_feature    12272..12278
                     /label="RE"
     misc_feature    12272..12278
                     /label="reRNA-preFlanking"
     primer          12272..12317
                     /label="ccdb_fwd"
                     /note="sequence: GGTTCAAcatcgcaggtgacaagtttgtacaaaaaagctgaacgag"
     misc_feature    12274..12278
                     /label="TTGAT (TAM)"
     gene            12523..13203
                     /label="CmR"
                     /Mismatches=0
                     /_Identity=100
                     /Motif="ATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGCGGGGCGTAATCTAGAGGATCCGGCTTACTAA"
                     /annotation_group="CmR: 3,651 -> 4,331"
     misc_feature    13204..13522
                     /label="ccdB 5' UTR"
                     /Mismatches=0
                     /_Identity=100
                     /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGATATGTATACCCGAAGTATGTCAAAAAGAGGTATGCTATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
                     /annotation_group="ccdB 5' UTR: 4,332 -> 4,650"
     misc_feature    13339..13340
                     /label="origin"
     misc_feature    13523..13828
                     /label="ccdB"
                     /Mismatches=0
                     /_Identity=100
                     /Motif="ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCCCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA"
                     /annotation_group="ccdB: 4,651 -> 4,956"
     primer          complement(13969..14004)
                     /label="ccdb_rev"
                     /note="sequence: catcgcaggtgaccactttgtacaagaaagctgaac"
     primer          13974..14022
                     /label="HDV&E9_fwd_adding_ccdb"
                     /note="sequence: gctttcttgtacaaagtggtcacctgcgatgggccggcatggtcccagc"
     primer          14005..14022
                     /label="HDV&E9_fwd_check"
                     /note="sequence: ggccggcatggtcccagc"
     misc_feature    14005..14072
                     /label="HDV"
     misc_feature    14073..14098
                     /label="Term_F"
                     /note="sequence: agcccgggcgagagctttcgttcgtatcatcggttt"
     terminator      14073..14707
                     /label="RbcS-E9t"
     source          14073..14707
                     /label="source"
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer          complement(14676..19)
                     /label="HDV&E9_rev_20547"
                     /note="sequence: cagctatgaccatgattacgaattcgttgtcaatcaattggcaagtcataaaatgca"
     misc_feature    complement(14676..14707)
                     /label="Term_R"
                     /note="sequence:ctcactgtcaccagagctctgttgtcaatcaattggcaagtcataaaatgca"
     primer_bind     complement(14676..14707)
                     /label=20547
                     /_Identity=100
                     /Motif="CAGCTATGACCATGATTACGAATTCGTTGTCAATCAATTGGCAAGTCATAAAATGCA"
                     /Sequence="CAGCTATGACCATGATTACGAATTCGTTGTCAATCAATTGGCAAGTCATAAAATGCA"
                     /Tm="66.9"
                     /GC="36.8"
                     /Hairpin_Tm="49.1"
                     /Self_Dimer_Tm="27.5"
                     /modified_by="cat"
     misc_feature    14708..14713
                     /label="EcoRI"
                     /Mismatches=0
                     /_Identity=100
                     /Motif="GAATTC"
                     /annotation_group="EcoRI: 14,164 -> 14,169"
     misc_feature    complement(14708..14713)
                     /label="EcoRI"
                     /Mismatches=0
                     /_Identity=100
                     /Motif="GAATTC"
                     /annotation_group="EcoRI: 14,164 <- 14,169"
     primer_bind     complement(14708..19)
                     /label=20547
                     /_Identity=100
                     /Motif="CAGCTATGACCATGATTACGAATTCGTTGTCAATCAATTGGCAAGTCATAAAATGCA"
                     /Sequence="CAGCTATGACCATGATTACGAATTCGTTGTCAATCAATTGGCAAGTCATAAAATGCA"
                     /Tm="66.9"
                     /GC="36.8"
                     /Hairpin_Tm="49.1"
                     /Self_Dimer_Tm="27.5"
                     /modified_by="cat"
ORIGIN
        1 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
       61 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
      121 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
      181 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attggctaga gcagcttgcc
      241 aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa
      301 gaccaaaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc
      361 cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg tggcacctac
      421 aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctgc cgacagtggt
      481 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg
      541 tcttcaaagc aagtggattg atgtgataac atggtggagc acgacactct cgtctactcc
      601 aagaatatca aagatacagt ctcagaagac caaagggcta ttgagacttt tcaacaaagg
      661 gtaatatcgg gaaacctcct cggattccat tgcccagcta tctgtcactt catcaaaagg
      721 acagtagaaa aggaaggtgg cacctacaaa tgccatcatt gcgataaagg aaaggctatc
      781 gttcaagatg cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc
      841 gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc
      901 actgacgtaa gggatgacgc acaatcccac tatccttcgc aagaccttcc tctatataag
      961 gaagttcatt tcatttggag aggacacgct gaaatcacca gtctctctct acaaatctat
     1021 ctctctcgag ctttcgcaga tcccgggggg caatgagata tgaaaaagcc tgaactcacc
     1081 gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag
     1141 ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc
     1201 ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt
     1261 gcatcggccg cgctcccgat tccggaagtg cttgacattg gggagtttag cgagagcctg
     1321 acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa
     1381 ctgcccgctg ttctacaacc ggtcgcggag gctatggatg cgatcgctgc ggccgatctt
     1441 agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg
     1501 cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac
     1561 gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac
     1621 tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac
     1681 aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac
     1741 gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc
     1801 tacttcgagc ggaggcatcc ggagcttgca ggatcgccac gactccgggc gtatatgctc
     1861 cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct
     1921 tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca
     1981 caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat
     2041 agtggaaacc gacgccccag cactcgtccg agggcaaaga aatagagtag atgccgaccg
     2101 gatctgtcga tcgacaagct cgagtttctc cataataatg tgtgagtagt tcccagataa
     2161 gggaattagg gttcctatag ggtttcgctc atgtgttgag catataagaa acccttagta
     2221 tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa aaccaaaatc
     2281 cagtactaaa atccagatcc cccgaattaa ttcggcgtta attcagtaca ttaaaaacgt
     2341 ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt acaccacaat atatcctgcc
     2401 accagccagc caacagctcc ccgaccggca gctcggcaca aaatcaccac tcgatacagg
     2461 cagcccatca gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc agactttgct
     2521 catgttaccg atgctattcg gaagaacggc aactaagctg ccgggtttga aacacggatg
     2581 atctcgcgga gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcac
     2641 cgcggtttca aaatcggctc cgtcgatact atgttatacg ccaactttga aaacaacttt
     2701 gaaaaagctg ttttctggta tttaaggttt tagaatgcaa ggaacagtga attggagttc
     2761 gtcttgttat aattagcttc ttggggtatc tttaaatact gtagaaaaga ggaaggaaat
     2821 aataaatggc taaaatgaga atatcaccgg aattgaaaaa actgatcgaa aaataccgct
     2881 gcgtaaaaga tacggaagga atgtctcctg ctaaggtata taagctggtg ggagaaaatg
     2941 aaaacctata tttaaaaatg acggacagcc ggtataaagg gaccacctat gatgtggaac
     3001 gggaaaagga catgatgcta tggctggaag gaaagctgcc tgttccaaag gtcctgcact
     3061 ttgaacggca tgatggctgg agcaatctgc tcatgagtga ggccgatggc gtcctttgct
     3121 cggaagagta tgaagatgaa caaagccctg aaaagattat cgagctgtat gcggagtgca
     3181 tcaggctctt tcactccatc gacatatcgg attgtcccta tacgaatagc ttagacagcc
     3241 gcttagccga attggattac ttactgaata acgatctggc cgatgtggat tgcgaaaact
     3301 gggaagaaga cactccattt aaagatccgc gcgagctgta tgatttttta aagacggaaa
     3361 agcccgaaga ggaacttgtc ttttcccacg gcgacctggg agacagcaac atctttgtga
     3421 aagatggcaa agtaagtggc tttattgatc ttgggagaag cggcagggcg gacaagtggt
     3481 atgacattgc cttctgcgtc cggtcgatca gggaggatat cggggaagaa cagtatgtcg
     3541 agctattttt tgacttactg gggatcaagc ctgattggga gaaaataaaa tattatattt
     3601 tactggatga attgttttag tacctagaat gcatgaccaa aatcccttaa cgtgagtttt
     3661 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3721 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3781 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3841 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3901 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3961 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     4021 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     4081 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     4141 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     4201 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     4261 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     4321 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     4381 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     4441 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc
     4501 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
     4561 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc
     4621 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     4681 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     4741 atcaccgaaa cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg
     4801 gcgcggcttg tccgcgccct ggtagattgc ctggccgtag gccagccatt tttgagcggc
     4861 cagcggccgc gataggccga cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg
     4921 taggcgcttt ttgcagctct tcggctgtgc gctggccaga cagttatgca caggccaggc
     4981 gggttttaag agttttaata agttttaaag agttttaggc ggaaaaatcg ccttttttct
     5041 cttttatatc agtcacttac atgtgtgacc ggttcccaat gtacggcttt gggttcccaa
     5101 tgtacgggtt ccggttccca atgtacggct ttgggttccc aatgtacgtg ctatccacag
     5161 gaaagagacc ttttcgacct ttttcccctg ctagggcaat ttgccctagc atctgctccg
     5221 tacattagga accggcggat gcttcgccct cgatcaggtt gcggtagcgc atgactagga
     5281 tcgggccagc ctgccccgcc tcctccttca aatcgtactc cggcaggtca tttgacccga
     5341 tcagcttgcg cacggtgaaa cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt
     5401 agatcgtctt gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt
     5461 agagaaaacg gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt
     5521 ccgggttctt gccttctgtg atctcgcggt acatccaatc agctagctcg atctcgatgt
     5581 actccggccg cccggtttcg ctctttacga tcttgtagcg gctaatcaag gcttcaccct
     5641 cggataccgt caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg
     5701 tggtgtttaa ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg ccatcggctc
     5761 gccggcagaa cttgagtacg tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc
     5821 ccttcccttc ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc atcagtacca
     5881 ggtcgtaatc ccacacactg gccatgccgg ccggccctgc ggaaacctct acgtgcccgt
     5941 ctggaagctc gtagcggatc acctcgccag ctcgtcggtc acgcttcgac agacggaaaa
     6001 cggccacgtc catgatgctg cgactatcgc gggtgcccac gtcatagagc atcggaacga
     6061 aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca ccggctgccg
     6121 gcggttgccg ggattctttg cggattcgat cagcggccgc ttgccacgat tcaccggggc
     6181 gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac ttctccacca
     6241 ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga tggtttgcga ccgctcacgc
     6301 cgattcctcg ggcttggggg ttccagtgcc attgcagggc cggcagacaa cccagccgct
     6361 tacgcctggc caaccgcccg ttcctccaca catggggcat tccacggcgt cggtgcctgg
     6421 ttgttcttga ttttccatgc cgcctccttt agccgctaaa attcatctac tcatttattc
     6481 atttgctcat ttactctggt agctgcgcga tgtattcaga tagcagctcg gtaatggtct
     6541 tgccttggcg taccgcgtac atcttcagct tggtgtgatc ctccgccggc aactgaaagt
     6601 tgacccgctt catggctggc gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc
     6661 gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt gctcattttc tctttacctc
     6721 attaactcaa atgagttttg atttaatttc agcggccagc gcctggacct cgcgggcagc
     6781 gtcgccctcg ggttctgatt caagaacggt tgtgccggcg gcggcagtgc ctgggtagct
     6841 cacgcgctgc gtgatacggg actcaagaat gggcagctcg tacccggcca gcgcctcggc
     6901 aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag
     6961 ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg cggtggccca
     7021 tatgtcgtaa gggcttggct gcaccggaat cagcacgaag tcggctgcct tgatcgcgga
     7081 cacagccaag tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc gccggccgat
     7141 ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc
     7201 ttcccgcacg gccgcccaat cgcgggcact gccctgggga tcggaatcga ctaacagaac
     7261 atcggccccg gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga
     7321 cccgcctttc tggttaagta cagcgataac cttcatgcgt tccccttgcg tatttgttta
     7381 tttactcatc gcatcatata cgcagcgacc gcatgacgca agctgtttta ctcaaataca
     7441 catcaccttt ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc
     7501 gccagcttgg catcagacaa accggccagg atttcatgca gccgcacggt tgagacgtgc
     7561 gcgggcggct cgaacacgta cccggccgcg atcatctccg cctcgatctc ttcggtaatg
     7621 aaaaacggtt cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat
     7681 tctcggcggc cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc
     7741 gcctggcctc ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc
     7801 gatgcacgcc aagcagtgca gccgcctctt tcacggtgcg gccttcctgg tcgatcagct
     7861 cgcgggcgtg cgcgatctgt gccggggtga gggtagggcg ggggccaaac ttcacgcctc
     7921 gggccttggc ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa cgctcgaact
     7981 cggcaatgcc ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc
     8041 acggctctgc caggctacgc aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca
     8101 gtaggtcgcg ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca acgtcgccag
     8161 ggcgtaggtg gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg ccggtgatct
     8221 tctcggaaaa cagcttggtg cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt
     8281 cctggtcgtc ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg ctcttgttca
     8341 tggcgtaatg tctccggttc tagtcgcaag tattctactt tatgcgacta aaacacgcga
     8401 caagaaaacg ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg
     8461 acatgtcgtt ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc
     8521 ggaggggttg gatcaaagta ctttgatccc gaggggaacc ctgtggttgg catgcacata
     8581 caaatggacg aacggataaa ccttttcacg cccttttaaa tatccgttat tctaataaac
     8641 gctcttttct cttaggttta cccgccaata tatcctgtca aacactgata gtttaaactg
     8701 aaggcgggaa acgacaatct gatccaagct caagctgctc tagcattcgc cattcaggct
     8761 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     8821 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     8881 ttgtaaaacg acggccagtg ccaagcttgc atgcctgcag gtcgactcta gatcactagt
     8941 atcctaggaa ggtaccagtc tagctcaaca gagcttttaa cccaaattgg tacaatagaa
     9001 tacaacttta gatcataatt ctcaaaagaa agagattcct tagctattct atctgccact
     9061 ccatttcctt ctcggcttgt atgcacaagc ataaaatcct caaacttgct aagtagatac
     9121 tttatgtctt ggataattgg attgagactt gacaagcata actttcatgt aaccaaagac
     9181 acaagttgct gagaatccac ctcaaaaatg atcttcctat aattgaatcg ggataatgac
     9241 agcacagccc atctaagagc ctccacttct acttccagca cgcttcttac ttttaccaca
     9301 gctcttgcac ctaaccataa caccttccct gtatgatcgc gaagcaccca ccctaagcca
     9361 cattttaatc cttctgttgg ccatgcccca tcaaagttgc acttaaccca agattgtggt
     9421 ggagcttccc atgtttctcg tctgtcccga cggtgttgtg gttggtgctt tccttacatt
     9481 ctgagcctct ttccttctaa tccactcatc tgcatcttct tgtgtcctta ctaatacctc
     9541 attggttcca aattccctcc ctttaagcac cagctcgttt ctgttcttcc acagcctccc
     9601 aagtatccaa gggactaaag cctccacatt cttcagatca ggatattctt gtttaagatg
     9661 ttgaactcta tggaggtttg tatgaactga tgatctagga ccggataagt tcccttcttc
     9721 atagcgaact tattcaaaga atgttttgtg tatcattctt gttacattgt tattaatgaa
     9781 aaaatattat tggtcattgg actgaacacg agtgttaaat atggaccagg ccccaaataa
     9841 gatccattga tatatgaatt aaataacaag aataaatcga gtcaccaaac cacttgcctt
     9901 ttttaacgag acttgttcac caacttgata caaaagtcat tatcctatgc aaatcaataa
     9961 tcatacaaaa atatccaata acactaaaaa attaaaagaa atggataatt tcacaatatg
    10021 ttatacgata aagaagttac ttttccaaga aattcactga ttttataagc ccacttgcat
    10081 tagataaatg gcaaaaaaaa acaaaaagga aaagaaataa agcacgaaga attctagaaa
    10141 atacgaaata cgcttcaatg cagtgggacc cacggttcaa ttattgccaa ttttcagctc
    10201 caccgtatat ttaaaaaata aaacgataat gctaaaaaaa tataaatcgt aacgatcgtt
    10261 aaatctcaac ggctggatct tatgacgacc gttagaaatt gtggttgtcg acgagtcagt
    10321 aataaacggc gtcaaagtgg ttgcagccgg cacacacgag tcgtgtttat caactcaaag
    10381 cacaaatact tttcctcaac ctaaaaataa ggcaattagc caaaaacaac tttgcgtgta
    10441 aacaacgctc aatacacgtg tcattttatt attagctatt gcttcaccgc cttagctttc
    10501 tcgtgaccta gtcgtcctcg tcttttcttc ttcttcttct ataaaacaat acccaaagag
    10561 ctcttcttct tcacaattca gatttcaatt tctcaaaatc ttaaaaactt tctctcaatt
    10621 ctctctaccg tgatcaaggt aaatttctgt gttccttatt ctctcaaaat cttcgatttt
    10681 gttttcgttc gatcccaatt tcgtatatgt tctttggttt agattctgtt aatcttagat
    10741 cgaagacgat tttctgggtt tgatcgttag atatcatctt aattctcgat tagggtttca
    10801 tagatatcat ccgatttgtt caaataattt gagttttgtc gaataattac tcttcgattt
    10861 gtgatttcta tctagatctg gtgttagttt ctagtttgtg cgatcgaatt tgtcgattaa
    10921 tctgagtttt tctgattaac agatggatta caaggatgat gatgataagg attacaagga
    10981 tgatgatgat aagatggctc caaagaagaa gagaaaggtt ggaatccacg gagttccagc
    11041 tgctATGATA AGGAATAAGG CTTTCGTGGT CAGGCTGTAC CCAAATGCGG CTCAGACTGA
    11101 ACTGATTAAC CGCACGCTGG GTAGCGCAAG GTTCGTCTAC AACCACTTCC TTGCCCGTCG
    11161 CATTGCGGCC TACAAGGAAA GCGGGAAGGG ACTGACCTAC GGGCAAACGA GTAGCGAACT
    11221 GACCCTTCTG AAGCAGGCTG AAGAAACCTC CTGGCTCTCG GAAGTAGATA AGTTTGCTTT
    11281 GCAGAACTCG CTGAAAAACC TTGAaACCGC GTACAAGAAC TTCTTTCGGA CTGTGAAGCA
    11341 GTCCGGTAAA AAGGTAGGAT TCCCACGTTT CAGAAAGAAG CGCACGGGAG AGTCCTACCG
    11401 GACTCAATTC ACCAACAACA ACATCCAAAT TGGGGAAGGT AGGCTCAAAC TTCCTAAGCT
    11461 GGGATGGGTG AAAACCAAGG GCCAGCAGGA TATTCAAGGG AAGATTCTGA ATGTCACTGT
    11521 GCGCCGTATT CACGAAGGCC ATTACGAAGC GTCCGTTCTC TGTGAAGTCG AGATTCCCTA
    11581 CCTGCCTGCG GCTCCCAAGT TTGCAGCGGG TGTGGATGTC GGCATCAAGG ATTTTGCCAT
    11641 CGTGACCGAT GGCGTGAGGT TTAAGCATGA ACAGAATCCG AAATATTACC GCTCCACCCT
    11701 GAAAAGACTT CGTAAAGCTC AGCAAACCCT GTCCAGACGG AAGAAGGGCA GCGCACGTTA
    11761 CGGGAAAGCG AAAACCAAGC TGGCTCGGAT TCACAAGCGC ATTGTCAATA AGCGTCAGGA
    11821 TTTCCTTCAC AAGCTCACCA CCTCCCTGGT GCGTGAGTAC GAAATCATCG GAACCGAACA
    11881 CCTTAAACCC GACAACATGC GGAAAAATCG CCGCCTTGCA CTGAGCATCA GTGATGCGGG
    11941 CTGGGGTGAG TTCATCCGGC AGTTGGAATA CAAGGCAGCG TGGTACGGGC GACTGGTATC
    12001 TAAAGTCAGC CCCTACTTTC CATCTAGCCA GTTGTGTCAT GACTGCGgat tcaagaatcc
    12061 cgaagtgaag aatcttgccg tccgtacatg gacttgcccg aactgtgggg aaacccatga
    12121 ccgagaCgag aacgctgcgc tgaacattcg gcgtgaagcg ttggtggctg cgggaatctc
    12181 agacacctta aacgctcatg gaggctatgt cagacctgct tcggcgggca atggtctgcg
    12241 aagtgagaat cacgcgactt tagtcgtgtg aGGTTCAAca tcgcaggtga caagtttgta
    12301 caaaaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt
    12361 gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac tatggcggcc
    12421 gcattaggaa ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg
    12481 agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
    12541 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
    12601 tcagtcgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
    12661 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
    12721 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
    12781 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
    12841 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
    12901 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
    12961 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
    13021 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
    13081 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
    13141 gaattacaac agtactgcga tgagtggcag gcggggcgta atctagagga tccggcttac
    13201 taaaagccag ataacagtat gcgtatttgc gcgctgattt ttgcggtata agaatatata
    13261 ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc gtattacagt
    13321 gacagttgac agcgacagct atcagttgct caaggcatat atgatgtcaa tatctccggt
    13381 ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg
    13441 gaaaatcagg aagggatggc tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct
    13501 gacgagaaca ggggctggtg aaatgcagtt taaggtttac acctataaaa gagagagccg
    13561 ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc gacggatggt
    13621 gatccccctg gccagtgcac gtctgctgtc agataaagtc ccccgtgaac tttacccggt
    13681 ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggc
    13741 ctccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc
    13801 cattaacctg atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtctgc
    13861 aggtcgacca tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat
    13921 gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt tcagctttct
    13981 tgtacaaagt ggtcacctgc gatgggccgg catggtccca gcctcctcgc tggcgccggc
    14041 tgggcaacat gcttcggcat ggcgaatggg acagagcttt cgttcgtatc atcggtttcg
    14101 acaacgttcg tcaagttcaa tgcatcagtt tcattgcgca cacaccagaa tcctactgag
    14161 tttgagtatt atggcattgg gaaaactgtt tttcttgtac catttgttgt gcttgtaatt
    14221 tactgtgttt tttattcggt tttcgctatc gaactgtgaa atggaaatgg atggagaaga
    14281 gttaatgaat gatatggtcc ttttgttcat tctcaaatta atattatttg ttttttctct
    14341 tatttgttgt gtgttgaatt tgaaattata agagatatgc aaacattttg ttttgagtaa
    14401 aaatgtgtca aatcgtggcc tctaatgacc gaagttaata tgaggagtaa aacacttgta
    14461 gttgtaccat tatgcttatt cactaggcaa caaatatatt ttcagaccta gaaaagctgc
    14521 aaatgttact gaatacaagt atgtcctctt gtgttttaga catttatgaa ctttccttta
    14581 tgtaattttc cagaatcctt gtcagattct aatcattgct ttataattat agttatactc
    14641 atggatttgt agttgagtat gaaaatattt tttaatgcat tttatgactt gccaattgat
    14701 tgacaacgaa ttc
//