LOCUS       pTW2154_CHLl1_g4_pMK43 12421 bp ds-DNA     linear       12-MAR-2025
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name:
            ptw2082_isymu1_g12_hdv_trna_ptw1101_pdk3888_trv2_pds3__149276.gb
            Imported using the Genbank importer. File name: 3888
            (TRV2-pPEBV-MCS-tRNAIleu).seq ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    19..766
                     /label="2x35S"
                     /locus_tag="2x35S"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    19..766
                     /label="New Feature"
                     /locus_tag="New Feature"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     primer          119..141
                     /label="virus_cas9_R4"
                     /note="sequence: tatcgggaaacctcctcggattc"
     primer          complement(256..276)
                     /label="virus_cas9_F4"
                     /note="sequence: gggtccatctttgggaccact"
     primer          450..472
                     /label="virus_cas9_R4"
                     /note="sequence: tatcgggaaacctcctcggattc"
     primer          complement(587..607)
                     /label="virus_cas9_F4"
                     /note="sequence: gggtccatctttgggaccact"
     misc_feature    770..1329
                     /label="TRV2-5' UTR"
                     /locus_tag="TRV2-5' UTR"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    1330..1944
                     /label="CP"
                     /locus_tag="CP"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     CDS             1330..1944
                     /label="CP"
                     /translation="MGDMYDESFDKSGGPADLMDDSWVESVSWKDLLKKLHSIKFALQSGRDEITGLLAALNRQCPYSPYEQFPDKKVYFLLDSRANSALGVIQNASAFKRRADEKNAVAGVTNIPANPNTTVTTNQGSTTTTKANTGSTLEEDLYTYYKFDDASTAFHKSLTSLENMELKSYYRRNFEKVFGIKFGGAAASSSAPPPASGGPIRPNP*"
     primer          1400..1448
                     /label="CP_F1"
                     /note="sequence: TGGAATCAGTTTCGTGGAAAGATCTGTTGAAGAAGTTACACAGCATAAA"
     primer          2061..2100
                     /label="TRV2_F_ZraI"
                     /note="sequence: gaatttcctttaccattgacgtcagtgtcgttggtagcat"
     misc_feature    2111..2345
                     /label="pPEBV"
                     /locus_tag="pPEBV"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    2318..2318
                     /label="New Feature"
                     /locus_tag="New Feature(1)"
                     /label="New Feature(1)"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     primer          complement(2321..2355)
                     /label="TW_19"
                     /note="sequence: tgtaatccatCTCGTTAACTCGGGTAAGTGATACA"
     primer          2336..2374
                     /label="TW_20"
                     /note="sequence: AGTTAACGAGatggattacaaggatgatgatgataagga"
     CDS             2346..2348
                     /label="ATG"
                     /product="<html><body>start codon</body></html>"
                     /translation="M"
     misc_feature    2346..2447
                     /label="misc"
                     /note="Geneious type: Editing History Insertion"
                     /Original_Bases=""
     CDS             2349..2396
                     /label="2xFLAG"
                     /product="<html><body>two tandem FLAG®epitopetags</body></html>"
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             2403..2423
                     /label="SV40 NLS"
                     /note="<html><body><br></body></html>"
                     /locus_tag="<html><body><br></body></html>"
                     /product="<html><body>nuclear localization signal ofSV40large T antigen</body></html>"
                     /protein_id="<html><body><br></body></html>"
                     /translation="PKKKRKV"
     misc_feature    2424..2447
                     /label=29451
     primer_bind     2428..2447
                     /label=26572
                     /Sequence="TCCACGGAGTTCCAGCTGCTATGTCGCAGACCATCACAGTCA"
                     /Tm="70.7"
                     /GC="54.8"
                     /Hairpin_Tm="47.2"
                     /Self_Dimer_Tm="12.7"
                     /created_by="cat"
     primer_bind     complement(2428..2447)
                     /label=20478
                     /GC="60.0"
                     /Hairpin_Tm="39.4"
                     /Self_Dimer_Tm="17.1"
                     /Tm="63.6"
                     /annotation_group="180399230532100409b548d-e1c4-410f-96c6-fed59a00bb7d"
                     /created_by="primer3"
                     /Sequence="AGCAGCTGGAACTCCGTGGA"
                     /Mismatches=0
     CDS             2448..3596
                     /label="ISYmu1 TnpB"
                     /note="codon_start: 1 transl_table: 1 translation: LLQHKAYEYRIYPDKKQETLIAKTIGSSRYVYNHFLELWNKEYEETGKGLTYYACSKLLTKLKRDPETVWLCEVDKFSLQNSLRNLSDAFSRFFKGQNEHPQFKSKKSPRQSYTTQYTNNNIAVSGNCLKLPKLGLVKFADSREMKGRILNATVRRKSSGKFFVSILCEEEICELPKTDSSVGIDLGIIDFAVMSDGSRHDNNHFTRQMEERLRREQRKLARRALAAEKRGISLSEARNYQKQRRKVARLYEKVANQRKEYLNKLSTEIVKNHDIICIEDLNVKGMMRNHKLAKSISDVSWTSLVSKLQYKASWYGKEVIRISRWFPSSQICSECGHKDRKKPLHVREWTCPVCHAHHDRDVNAARNILAEGLRIRALTPGS*"
                     /translation="MLQHKAYEYRIYPDKKQETLIAKTIGSSRYVYNHFLELWNKEYEETGKGLTYYACSKLLTKLKRDPETVWLCEVDKFSLQNSLRNLSDAFSRFFKGQNEHPQFKSKKSPRQSYTTQYTNNNIAVSGNCLKLPKLGLVKFADSREMKGRILNATVRRKSSGKFFVSILCEEEICELPKTDSSVGIDLGIIDFAVMSDGSRHDNNHFTRQMEERLRREQRKLARRALAAEKRGISLSEARNYQKQRRKVARLYEKVANQRKEYLNKLSTEIVKNHDIICIEDLNVKGMMRNHKLAKSISDVSWTSLVSKLQYKASWYGKEVIRISRWFPSSQICSECGHKDRKKPLHVREWTCPVCHAHHDRDVNAARNILAEGLRIRALTPGS*"
     source          2448..3734
                     /label="source"
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     misc_feature    3535..3734
                     /label="200-nt wRNA"
     primer          3751..3770
                     /label="g2_SDM_F"
                     /note="sequence: ggccggcatggtcccagcct"
     misc_feature    3751..3818
                     /label="FROM pTW1395"
     stem_loop       3751..3818
                     /label="HDV ribozyme"
     misc_feature    3758..3775
                     /label="HDV_FWD"
     primer          complement(3775..3788)
                     /label="ISDra2_F_C-T_BanI"
                     /note="sequence: cagccggcgccagc"
     misc_feature    complement(3788..3806)
                     /label="ccdb_REV"
     primer          complement(3802..3838)
                     /label="TnpB_R_2"
                     /note="sequence: TCTAACCAACTGAGCTACGGgtcccattcgccatgcc"
     primer          3804..3840
                     /label="pTW1101_F_2"
                     /note="sequence: catggcgaatgggacCCGTAGCTCAGTTGGTTAGAGC"
     misc_feature    3819..3888
                     /label="tRNAIleu"
                     /locus_tag="tRNAIleu"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     primer          complement(3874..3908)
                     /label="tRNA_R"
                     /note="sequence: GTCTTCGGGACATGCCCGGGTGCTTCCGGCGGGGC"
     primer          complement(3877..3911)
                     /label="tRNA_R_3"
                     /note="sequence: AATGTCTTCGGGACATGCCCGGGTGCTTCCGGCGG"
     misc_feature    3896..4290
                     /label="3'UTR"
                     /locus_tag="3'UTR"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    complement(3897..3925)
                     /label="HDV_REV"
     primer          complement(4251..4292)
                     /label="R_3888_4"
                     /note="sequence: ACGGGCGTAATAACGCTTACGTAGGCGAGGGGTTTTACCCCT"
     primer          complement(4252..4292)
                     /label="R_3888_3"
                     /note="sequence: ACGGGCGTAATAACGCTTACGTAGGCGAGGGGTTTTACCCC"
     misc_feature    4291..4339
                     /label="Ribozyme"
                     /locus_tag="Ribozyme"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     primer          complement(4352..4388)
                     /label="pTW1101_R_1"
                     /note="sequence: TGGTCACCTGTAATTCACACGTGGTGGTGGTGGTGGT"
     misc_feature    4403..4657
                     /label="NOSterm"
                     /locus_tag="NOSterm"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     primer          complement(4432..4457)
                     /label="virus_cas9_F"
                     /note="sequence: gaccggcaacaggattcaatcttaag"
     misc_feature    4677..4721
                     /label="RB"
                     /locus_tag="RB"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    4729..4729
                     /label="New Feature"
                     /locus_tag="New Feature(2)"
                     /label="New Feature(2)"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    4731..4731
                     /label="New Feature"
                     /locus_tag="New Feature(3)"
                     /label="New Feature(3)"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    9715..11057
                     /label="IS element"
                     /locus_tag="IS element"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    11076..11870
                     /label="Kan"
                     /locus_tag="Kan"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    12295..12320
                     /label="LB"
                     /locus_tag="LB"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
ORIGIN
        1 AAGCTTGCAT GCCTGCAGGT CAACATGGTG GAGCACGACA CTCTCGTCTA CTCCAAGAAT
       61 ATCAAAGATA CAGTCTCAGA AGACCAGAGG GCTATTGAGA CTTTTCAACA AAGGGTAATA
      121 TCGGGAAACC TCCTCGGATT CCATTGCCCA GCTATCTGTC ACTTCATCGA AAGGACAGTA
      181 GAAAAGGAAG ATGGCTTCTA CAAATGCCAT CATTGCGATA AAGGAAAGGC TATCGTTCAA
      241 GATGCCTCTA CCGACAGTGG TCCCAAAGAT GGACCCCCAC CCACGAGGAA CATCGTGGAA
      301 AAAGAAGACG TTCCAACCAC GTCTTCAAAG CAAGTGGATT GATGTGATGG TCAACATGGT
      361 GGAGCACGAC ACTCTCGTCT ACTCCAAGAA TATCAAAGAT ACAGTCTCAG AAGACCAGAG
      421 GGCTATTGAG ACTTTTCAAC AAAGGGTAAT ATCGGGAAAC CTCCTCGGAT TCCATTGCCC
      481 AGCTATCTGT CACTTCATCG AAAGGACAGT AGAAAAGGAA GATGGCTTCT ACAAATGCCA
      541 TCATTGCGAT AAAGGAAAGG CTATCGTTCA AGATGCCTCT ACCGACAGTG GTCCCAAAGA
      601 TGGACCCCCA CCCACGAGGA ACATCGTGGA AAAAGAAGAC GTTCCAACCA CGTCTTCAAA
      661 GCAAGTGGAT TGATGTGATA TCTCCACTGA CGTAAGGGAT GACGCACAAT CCCACTATCC
      721 TTCGCAAGAC CCTTCCTCTA TATAAGGAAG TTCATTTCAT TTGGAGAGGA TAAAACATTG
      781 CACCTATGGT GTTGCCCTGG CTGGGGTATG TCAGTGATCG CAGTAGAATG TACTAATTGA
      841 CAAGTTGGAG AATACGGTAG AACGTCCTTA TCCAACACAG CCTTTATCCC TCTCCCTGAC
      901 GAGGTTTTTG TCAGTGTAAT ATTTCTTTTT GAACTATCCA GCTTAGTACC GTACGGGAAA
      961 GTGACTGGTG TGCTTATCTT TGAAATGTTA CTTTGGGTTT CGGTTCTTTA GGTTAGTAAG
     1021 AAAGCACTTG TCTTCTCATA CAAAGGAAAA CCTGAGACGT ATCGCTTACG AAAGTAGCAA
     1081 TGAAAGAAAG GTGGTGGTTT TAATCGCTAC CGCAAAAACG ATGGGGTCGT TTTAATTAAC
     1141 TTCTCCTACG CAAGCGTCTA AACGGACGTT GGGGTTTTGC TAGTTTCTTT AGAGAAAACT
     1201 AGCTAAGTCT TTAATGTTAT CATTAGAGAT GGCATAAATA TAATACTTGT GTCTGCTGAT
     1261 AAGATCATTT TAATTTGGAC GATTAGACTT GTTGAACTAC AGGTTACTGA ATCACTTGCG
     1321 CTAATCAACA TGGGAGATAT GTACGATGAA TCATTTGACA AGTCGGGCGG TCCTGCTGAC
     1381 TTGATGGACG ATTCTTGGGT GGAATCAGTT TCGTGGAAAG ATCTGTTGAA GAAGTTACAC
     1441 AGCATAAAAT TTGCACTACA GTCTGGTAGA GATGAGATCA CTGGGTTACT AGCGGCACTG
     1501 AATAGACAGT GTCCTTATTC ACCATATGAG CAGTTTCCAG ATAAGAAGGT GTATTTCCTT
     1561 TTAGACTCAC GGGCTAACAG TGCTCTTGGT GTGATTCAGA ACGCTTCAGC GTTCAAGAGA
     1621 CGAGCTGATG AGAAGAATGC AGTGGCGGGT GTTACAAATA TTCCTGCGAA TCCAAACACA
     1681 ACGGTTACGA CGAACCAAGG GAGTACTACT ACTACCAAGG CGAACACTGG CTCGACTTTG
     1741 GAAGAAGACT TGTACACTTA TTACAAATTC GATGATGCCT CTACAGCTTT CCACAAATCT
     1801 CTAACTTCGT TAGAGAACAT GGAGTTGAAG AGTTATTACC GAAGGAACTT TGAGAAAGTA
     1861 TTCGGGATTA AGTTTGGTGG AGCAGCTGCT AGTTCATCTG CACCGCCTCC AGCGAGTGGA
     1921 GGTCCGATAC GTCCTAATCC CTAGggattt aaggacgtga actctgttga gatctctgtg
     1981 aaattcagag ggtgggtgat accatattca ctgatgccat tagcgacatc taaatagggc
     2041 taattgtgac taatttgagg gaatttcctt taccattgac gtcagtgtcg ttggtagcat
     2101 ttgagtttcg GCACACAAGG TTAAAAACGC TGTAGTAATA CATGCGCAAG AACAGGCTGA
     2161 GCATCTTGTT CTGGGGTTTC ACACTATCTT TAGAGAAAGT GTTAAGTTAA TTAAGTTATC
     2221 TTAATTAAGA GCATAATTAT ACTGATTTGT CTCTCGTTGA TAGAGTCTAT CATTCTGTTA
     2281 TTAAAAATTT GACAACTCGG TTTGCTGACC TACTGGTCAC TGTATCACTT ACCCGAGTTA
     2341 ACGAGatgga ttacaaggat gatgatgata aggattacaa ggatgatgat gataagatgg
     2401 ctccaaagaa gaagagaaag gttggaatcc acggagttcc agctgctATG CTGCAGCATA
     2461 AAGCCTATGA ATACCGTATC TATCCAGATA AGAAGCAGGA AACACTGATT GCCAAGACAA
     2521 TAGGCAGTTC AAGGTATGTA TACAACCATT TCCTGGAACT TTGGAACAAG GAATATGAGG
     2581 AGACCGGAAA AGGTCTGACT TATTACGCAT GCTCCAAACT TCTGACAAAG CTTAAGAGGG
     2641 ACCCGGAAAC AGTATGGCTT TGTGAAGTGG ATAAGTTCTC GCTCCAGAAT TCGCTTCGTA
     2701 ACCTGTCGGA CGCCTTCTCC CGCTTTTTCA AAGGACAGAA CGAGCATCCC CAGTTCAAGA
     2761 GCAAGAAGAG CCCGAGGCAA AGCTACACGA CACAATATAC GAACAACAAC ATCGCAGTTT
     2821 CCGGAAATTG TCTGAAGCTG CCTAAGCTGG GCCTTGTCAA GTTTGCAGAC AGTAGGGAGA
     2881 TGAAAGGCCG TATACTGAAT GCCACAGTAC GAAGGAAATC GAGCGGGAAA TTCTTCGTAT
     2941 CGATCCTATG CGAAGAGGAG ATCTGTGAAT TGCCAAAGAC TGACTCATCT GTCGGAATTG
     3001 ACCTCGGAAT CATTGACTTT GCGGTTATGT CAGACGGAAG CAGGCATGAC AACAATCATT
     3061 TTACCAGGCA AATGGAAGAA AGGCTTAGAC GGGAGCAGCG AAAGCTTGCA AGGCGTGCAC
     3121 TTGCTGCGGA GAAAAGGGGC ATTTCCCTTT CTGAAGCCAG GAACTATCAG AAGCAAAGGC
     3181 GGAAGGTAGC GAGACTTTAT GAAAAGGTTG CAAACCAGCG AAAAGAATAT CTTAACAAAC
     3241 TCAGTACAGA GATAGTCAAA AACCACGATA TCATCTGTAT CGAGGACCTT AACGTTAAGG
     3301 GCATGATGCG CAATCATAAA CTGGCAAAAA GCATCTCTGA TGTATCATGG ACGAGCCTTG
     3361 TATCGAAACT GCAGTACAAG GCTTCCTGGT ATGGAAAAGA AGTCATCAGG ATAAGCAGAT
     3421 GGTTTCCGTC AAGTCAGATA TGCTCAGAGT GCGGCCACAA GGACAGGAAG AAGCCTCTCC
     3481 ATGTAAGGGA GTGGACCTGT CCTGTTTGTC ATGCCCACCA TGACCGTGAC GTCAATGCAG
     3541 CAAGAAATAT ACTGGCTGAG GGACTCAGGA TAAGAGCTCT GACTCCGGGG TCTTAGCAAT
     3601 ACAGAAGCAA ACAGGAACCG CAGGAATTGC GGGGGTAGCT TGGTAAACAA GAGAAACCTC
     3661 TGCCGGCAAA GAAATAAGCC GGTAAGTATG CTCTGTTCCC AAGAATCTCG TGACTTTAGT
     3721 CATGAGAGTT TCAACTGTTA CCTGAGATTA ggccggcatg gtcccagcct cctcgctggc
     3781 gccggctggg caacatgctt cggcatggcg aatgggacCC GTAGCTCAGT TGGTTAGAGC
     3841 GTTGGTCTTA TGAGCCGAAG GTCGCGGGTT CGAGCCCCGC CGGAAGCACC CGGGCATGTC
     3901 CCGAAGACAT TAAACTACGG TTCTTTAAGT AGATCCGTGT CTGAAGTTTT AGGTTCAATT
     3961 TAAACCTACG AGATTGACAT TCTCGACTGA TCTTGATTGA TCGGTAAGTC TTTTGTAATT
     4021 TAATTTTCTT TTTGATTTTA TTTTAAATTG TTATCTGTTT CTGTGTATAG ACTGTTTGAG
     4081 ATCGGCGTTT GGCCGACTCA TTGTCTTACC ATAGGGGAAC GGACTTTGTT TGTGTTGTTA
     4141 TTTTATTTGT ATTTTATTAA AATTCTCAAC GATCTGAAAA AGCCTCGCGG CTAAGAGATT
     4201 GTTGGGGGGT GAGTAAGTAC TTTTAAAGTG ATGATGGTTA CAAAGGCAAA AGGGGTAAAA
     4261 CCCCTCGCCT ACGTAAGCGT TATTACGCCC GTCTGTACTT ATATCAGTAC ACTGACGAGT
     4321 CCCTAAAGGA CGAAACGGGA GAACGCTAGC CACCACCACC ACCACCACGT GTGAATTACA
     4381 GGTGACCAGC TCGAATTTCC CCGATCGTTC AAACATTTGG CAATAAAGTT TCTTAAGATT
     4441 GAATCCTGTT GCCGGTCTTG CGATGATTAT CATATAATTT CTGTTGAATT ACGTTAAGCA
     4501 TGTAATAATT AACATGTAAT GCATGACGTT ATTTATGAGA TGGGTTTTTA TGATTAGAGT
     4561 CCCGCAATTA TACATTTAAT ACGCGATAGA AAACAAAATA TAGCGCGCAA ACTAGGATAA
     4621 ATTATCGCGC GCGGTGTCAT CTATGTTACT AGATCGGGAA TTAAACTATC AGTGTTTGAC
     4681 AGGATATATT GGCGGGTAAA CCTAAGAGAA AAGAGCGTTT ATTAGAATAA TCGGATATTT
     4741 AAAAGGGCGT GAAAAGGTTT ATCCGTTCGT CCATTTGTAT GTGCATGCCA ACCACAGGGT
     4801 TCCCCTCGGG ATCAAAGTAC TTTGATCCAA CCCCTCCGCT GCTATAGTGC AGTCGGCTTC
     4861 TGACGTTCAG TGCAGCCGTC TTCTGAAAAC GACATGTCGC ACAAGTCCTA AGTTACGCGA
     4921 CAGGCTGCCG CCCTGCCCTT TTCCTGGCGT TTTCTTGTCG CGTGTTTTAG TCGCATAAAG
     4981 TAGAATACTT GCGACTAGAA CCGGAGACAT TACGCCATGA ACAAGAGCGC CGCCGCTGGC
     5041 CTGCTGGGCT ATGCCCGCGT CAGCACCGAC GACCAGGACT TGACCAACCA ACGGGCCGAA
     5101 CTGCACGCGG CCGGCTGCAC CAAGCTGTTT TCCGAGAAGA TCACCGGCAC CAGGCGCGAC
     5161 CGCCCGGAGC TGGCCAGGAT GCTTGACCAC CTACGCCCTG GCGACGTTGT GACAGTGACC
     5221 AGGCTAGACC GCCTGGCCCG CAGCACCCGC GACCTACTGG ACATTGCCGA GCGCATCCAG
     5281 GAGGCCGGCG CGGGCCTGCG TAGCCTGGCA GAGCCGTGGG CCGACACCAC CACGCCGGCC
     5341 GGCCGCATGG TGTTGACCGT GTTCGCCGGC ATTGCCGAGT TCGAGCGTTC CCTAATCATC
     5401 GACCGCACCC GGAGCGGGCG CGAGGCCGCC AAGGCCCGAG GCGTGAAGTT TGGCCCCCGC
     5461 CCTACCCTCA CCCCGGCACA GATCGCGCAC GCCCGCGAGC TGATCGACCA GGAAGGCCGC
     5521 ACCGTGAAAG AGGCGGCTGC ACTGCTTGGC GTGCATCGCT CGACCCTGTA CCGCGCACTT
     5581 GAGCGCAGCG AGGAAGTGAC GCCCACCGAG GCCAGGCGGC GCGGTGCCTT CCGTGAGGAC
     5641 GCATTGACCG AGGCCGACGC CCTGGCGGCC GCCGAGAATG AACGCCAAGA GGAACAAGCA
     5701 TGAAACCGCA CCAGGACGGC CAGGACGAAC CGTTTTTCAT TACCGAAGAG ATCGAGGCGG
     5761 AGATGATCGC GGCCGGGTAC GTGTTCGAGC CGCCCGCGCA CGTCTCAACC GTGCGGCTGC
     5821 ATGAAATCCT GGCCGGTTTG TCTGATGCCA AGCTGGCGGC CTGGCCGGCC AGCTTGGCCG
     5881 CTGAAGAAAC CGAGCGCCGC CGTCTAAAAA GGTGATGTGT ATTTGAGTAA AACAGCTTGC
     5941 GTCATGCGGT CGCTGCGTAT ATGATGCGAT GAGTAAATAA ACAAATACGC AAGGGGAACG
     6001 CATGAAGGTT ATCGCTGTAC TTAACCAGAA AGGCGGGTCA GGCAAGACGA CCATCGCAAC
     6061 CCATCTAGCC CGCGCCCTGC AACTCGCCGG GGCCGATGTT CTGTTAGTCG ATTCCGATCC
     6121 CCAGGGCAGT GCCCGCGATT GGGCGGCCGT GCGGGAAGAT CAACCGCTAA CCGTTGTCGG
     6181 CATCGACCGC CCGACGATTG ACCGCGACGT GAAGGCCATC GGCCGGCGCG ACTTCGTAGT
     6241 GATCGACGGA GCGCCCCAGG CGGCGGACTT GGCTGTGTCC GCGATCAAGG CAGCCGACTT
     6301 CGTGCTGATT CCGGTGCAGC CAAGCCCTTA CGACATATGG GCCACCGCCG ACCTGGTGGA
     6361 GCTGGTTAAG CAGCGCATTG AGGTCACGGA TGGAAGGCTA CAAGCGGCCT TTGTCGTGTC
     6421 GCGGGCGATC AAAGGCACGC GCATCGGCGG TGAGGTTGCC GAGGCGCTGG CCGGGTACGA
     6481 GCTGCCCATT CTTGAGTCCC GTATCACGCA GCGCGTGAGC TACCCAGGCA CTGCCGCCGC
     6541 CGGCACAACC GTTCTTGAAT CAGAACCCGA GGGCGACGCT GCCCGCGAGG TCCAGGCGCT
     6601 GGCCGCTGAA ATTAAATCAA AACTCATTTG AGTTAATGAG GTAAAGAGAA AATGAGCAAA
     6661 AGCACAAACA CGCTAAGTGC CGGCCGTCCG AGCGCACGCA GCAGCAAGGC TGCAACGTTG
     6721 GCCAGCCTGG CAGACACGCC AGCCATGAAG CGGGTCAACT TTCAGTTGCC GGCGGAGGAT
     6781 CACACCAAGC TGAAGATGTA CGCGGTACGC CAAGGCAAGA CCATTACCGA GCTGCTATCT
     6841 GAATACATCG CGCAGCTACC AGAGTAAATG AGCAAATGAA TAAATGAGTA GATGAATTTT
     6901 AGCGGCTAAA GGAGGCGGCA TGGAAAATCA AGAACAACCA GGCACCGACG CCGTGGAATG
     6961 CCCCATGTGT GGAGGAACGG GCGGTTGGCC AGGCGTAAGC GGCTGGGTTG CCTGCCGGCC
     7021 CTGCAATGGC ACTGGAACCC CCAAGCCCGA GGAATCGGCG TGAGCGGTCG CAAACCATCC
     7081 GGCCCGGTAC AAATCGGCGC GGCGCTGGGT GATGACCTGG TGGAGAAGTT GAAGGCCGCG
     7141 CAGGCCGCCC AGCGGCAACG CATCGAGGCA GAAGCACGCC CCGGTGAATC GTGGCAAGCG
     7201 GCCGCTGATC GAATCCGCAA AGAATCCCGG CAACCGCCGG CAGCCGGTGC GCCGTCGATT
     7261 AGGAAGCCGC CCAAGGGCGA CGAGCAACCA GATTTTTTCG TTCCGATGCT CTATGACGTG
     7321 GGCACCCGCG ATAGTCGCAG CATCATGGAC GTGGCCGTTT TCCGTCTGTC GAAGCGTGAC
     7381 CGACGAGCTG GCGAGGTGAT CCGCTACGAG CTTCCAGACG GGCACGTAGA GGTTTCCGCA
     7441 GGGCCGGCCG GCATGGCCAG TGTGTGGGAT TACGACCTGG TACTGATGGC GGTTTCCCAT
     7501 CTAACCGAAT CCATGAACCG ATACCGGGAA GGGAAGGGAG ACAAGCCCGG CCGCGTGTTC
     7561 CGTCCACACG TTGCGGACGT ACTCAAGTTC TGCCGGCGAG CCGATGGCGG AAAGCAGAAA
     7621 GACGACCTGG TAGAAACCTG CATTCGGTTA AACACCACGC ACGTTGCCAT GCAGCGTACG
     7681 AAGAAGGCCA AGAACGGCCG CCTGGTGACG GTATCCGAGG GTGAAGCCTT GATTAGCCGC
     7741 TACAAGATCG TAAAGAGCGA AACCGGGCGG CCGGAGTACA TCGAGATCGA GCTAGCTGAT
     7801 TGGATGTACC GCGAGATCAC AGAAGGCAAG AACCCGGACG TGCTGACGGT TCACCCCGAT
     7861 TACTTTTTGA TCGATCCCGG CATCGGCCGT TTTCTCTACC GCCTGGCACG CCGCGCCGCA
     7921 GGCAAGGCAG AAGCCAGATG GTTGTTCAAG ACGATCTACG AACGCAGTGG CAGCGCCGGA
     7981 GAGTTCAAGA AGTTCTGTTT CACCGTGCGC AAGCTGATCG GGTCAAATGA CCTGCCGGAG
     8041 TACGATTTGA AGGAGGAGGC GGGGCAGGCT GGCCCGATCC TAGTCATGCG CTACCGCAAC
     8101 CTGATCGAGG GCGAAGCATC CGCCGGTTCC TAATGTACGG AGCAGATGCT AGGGCAAATT
     8161 GCCCTAGCAG GGGAAAAAGG TCGAAAAGGT CTCTTTCCTG TGGATAGCAC GTACATTGGG
     8221 AACCCAAAGC CGTACATTGG GAACCGGAAC CCGTACATTG GGAACCCAAA GCCGTACATT
     8281 GGGAACCGGT CACACATGTA AGTGACTGAT ATAAAAGAGA AAAAAGGCGA TTTTTCCGCC
     8341 TAAAACTCTT TAAAACTTAT TAAAACTCTT AAAACCCGCC TGGCCTGTGC ATAACTGTCT
     8401 GGCCAGCGCA CAGCCGAAGA GCTGCAAAAA GCGCCTACCC TTCGGTCGCT GCGCTCCCTA
     8461 CGCCCCGCCG CTTCGCGTCG GCCTATCGCG GCCGCTGGCC GCTCAAAAAT GGCTGGCCTA
     8521 CGGCCAGGCA ATCTACCAGG GCGCGGACAA GCCGCGCCGT CGCCACTCGA CCGCCGGCGC
     8581 CCACATCAAG GCACCCTGCC TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT
     8641 GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG
     8701 TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCGCA GCCATGACCC AGTCACGTAG
     8761 CGATAGCGGA GTGTATACTG GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG
     8821 CACCATATGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGC
     8881 TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA
     8941 TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG
     9001 AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG
     9061 TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG
     9121 TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG
     9181 CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA
     9241 AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC
     9301 TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT
     9361 AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT
     9421 GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG
     9481 CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT
     9541 ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT
     9601 GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT
     9661 TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGctgatg
     9721 aatcccctaa tgatttttat caaaatcatt aagttaaggt agatacacat cttgtcatat
     9781 gatcaaatgg tttcgccaaa aatcaataat cagacaacaa aatgtgcgaa ctcgatattt
     9841 tacacgactc tctttaccaa ttctgccccg aattacactt aaaacgactc aacagcttaa
     9901 cgttggcttg ccacgcctta cttgactgta aaactctcac tcttaccgaa cttggccgta
     9961 acctgccaac caaagcgaga acaaaacata acatcaaacg aatcgaccga ttgttaggta
    10021 atcgtcacct ccacaaagag cgactcgctg tataccgttg gcatgctagc tttatctgtt
    10081 cgggcaatac gatgcccatt gtacttgttg actggtctga tatccgtgag caaaaacggc
    10141 ttatggtatt gcgagcttca gtcgcactac acggtcgttc tgttactctt tatgagaaag
    10201 cgttcccgct ttcagagcaa tgttcaaaga aagctcatga ccaatttcta gccgaccttg
    10261 cgagcattct accgagtaac accacaccgc tcattgtcag tgatgctggc tttaaagtgc
    10321 catggtataa atccgttgag aagctgggtt ggtactggtt aagtcgagta agaggaaaag
    10381 tacaatatgc agacctagga gcggaaaact ggaaacctat cagcaactta catgatatgt
    10441 catctagtca ctcaaagact ttaggctata agaggctgac taaaagcaat ccaatctcat
    10501 gccaaattct attgtataaa tctcgctcta aaggccgaaa aaatcagcgc tcgacacgga
    10561 ctcattgtca ccacccgtca cctaaaatct actcagcgtc ggcaaaggag ccatggattc
    10621 tagcaactaa cttacctgtt gaaattcgaa cacccaaaca acttgttaat atctattcga
    10681 agcgaatgca gattgaagaa accttccgag acttgaaaag tcctgcctac ggactaggcc
    10741 tacgccatag ccgaacgagc agctcagagc gttttgatat catgctgcta atcgccctga
    10801 tgcttcaact aacatgttgg cttgcgggcg ttcatgctca gaaacaaggt tgggacaagc
    10861 acttccaggc taacacagtc agaaatcgaa acgtactctc aacagttcgc ttaggcatgg
    10921 aagttttgcg gcattctggc tacacaataa caagggaaga ctcactcgtg gctgcaaccc
    10981 tgcttactca aaatctattc acacatggtt acgttttggg gaaattatga ggggatctct
    11041 cagcgttaag ggattttGGT CATGCATTCT AGGTACTAAA ACAATTCATC CAGTAAAATA
    11101 TAATATTTTA TTTTCTCCCA ATCAGGCTTG ATCCCCAGTA AGTCAAAAAA TAGCTCGACA
    11161 TACTGTTCTT CCCCGATATC CTCCCTGATC GACCGGACGC AGAAGGCAAT GTCATACCAC
    11221 TTGTCCGCCC TGCCGCTTCT CCCAAGATCA ATAAAGCCAC TTACTTTGCC ATCTTTCACA
    11281 AAGATGTTGC TGTCTCCCAG GTCGCCGTGG GAAAAGACAA GTTCCTCTTC GGGCTTTTCC
    11341 GTCTTTAAAA AATCATACAG CTCGCGCGGA TCTTTAAATG GAGTGTCTTC TTCCCAGTTT
    11401 TCGCAATCCA CATCGGCCAG ATCGTTATTC AGTAAGTAAT CCAATTCGGC TAAGCGGCTG
    11461 TCTAAGCTAT TCGTATAGGG ACAATCCGAT ATGTCGATGG AGTGAAAGAG CCTGATGCAC
    11521 TCCGCATACA GCTCGATAAT CTTTTCAGGG CTTTGTTCAT CTTCATACTC TTCCGAGCAA
    11581 AGGACGCCAT CGGCCTCACT CATGAGCAGA TTGCTCCAGC CATCATGCCG TTCAAAGTGC
    11641 AGGACCTTTG GAACAGGCAG CTTTCCTTCC AGCCATAGCA TCATGTCCTT TTCCCGTTCC
    11701 ACATCATAGG TGGTCCCTTT ATACCGGCTG TCCGTCATTT TTAAATATAG GTTTTCATTT
    11761 TCTCCCACCA GCTTATATAC CTTAGCAGGA GACATTCCTT CCGTATCTTT TACGCAGCGG
    11821 TATTTTTCGA TCAGTTTTTT CAATTCCGGT GATATTCTCA TTTTAGCCAT TTATTATTTC
    11881 CTTCCTCTTT TCTACAGTAT TTAAAGATAC CCCAAGAAGC TAATTATAAC AAGACGAACT
    11941 CCAATTCACT GTTCCTTGCA TTCTAAAACC TTAAATACCA GAAAACAGCT TTTTCAAAGT
    12001 TGTTTTCAAA GTTGGCGTAT AACATAGTAT CGACGGAGCC GATTTTGAAA CCGCGGTGAT
    12061 CACAGGCAGC AACGCTCTGT CATCGTTACA ATCAACATGC TACCCTCCGC GAGATCATCC
    12121 GTGTTTCAAA CCCGGCAGCT TAGTTGCCGT TCTTCCGAAT AGCATCGGTA ACATGAGCAA
    12181 AGTCTGCCGC CTTACAACGG CTCTCCCGCT GACGCCGTCC CGGACTGATG GGCTGCCTGT
    12241 ATCGAGTGGT GATTTTGTGC CGAGCTGCCG GTCGGGGAGC TGTTGGCTGG CTGGTGGCAG
    12301 GATATATTGT GGTGTAAACA AATTGACGCT TAGACAACTT AATAACACAT TGCGGACGTT
    12361 TTTAATGTAC TGAATTAACG CCGAATTAAT TCCTAGGCCA CCATGTTGGG CCCGGCGCGC
    12421 C
//