LOCUS       CN2701\pAAV-hChATp(894bp)-SYFP2-WPRE3-BGHpA\(JT)        5072 bp    DNA     circular      7-JUL-2020
SOURCE      
  ORGANISM  
COMMENT     rAAV-hPTenh(FAM84b)-minBG-SYFP2-WPRE3-BGHpA
            
            clones 2,3,4 all good
            clone 1 low yield 
            
            
            
            
            
            
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-13418805|
COMMENT     VNTDBDATE|-13418805|
COMMENT     LSOWNER|
COMMENT     VNTNAME|CN2701 pAAV-hChATp(894bp)-SYFP2-WPRE3-BGHpA (JT)|
COMMENT     VNTAUTHORNAME|Jonathan Ting|
COMMENT     VNTOAUTHORNAME|Feng Zhang|
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /vntifkey="21"
                     /label=L-ITR
     misc_feature    2335..2475
                     /vntifkey="21"
                     /label=R-ITR
     rep_origin      2567..2873
                     /vntifkey="33"
                     /label=f1\Origin
     misc_feature    3392..4249
                     /vntifkey="21"
                     /label=AmpR
     rep_origin      4400..5067
                     /vntifkey="33"
                     /label=pUC\ori
     primer          complement(2217..2236)
                     /vntifkey="27"
                     /label=BGH-R
     misc_feature    2103..2306
                     /vntifkey="21"
                     /label=BGH-pA
     misc_feature    1844..2089
                     /vntifkey="21"
                     /label=WPRE3
     misc_feature    1844..2003
                     /vntifkey="21"
                     /label=gamma
     misc_feature    2009..2089
                     /vntifkey="21"
                     /label=alpha
     misc_feature    complement(1957..1976)
                     /vntifkey="21"
                     /label=WPRE3-R\(JT)
     misc_feature    1090..1809
                     /vntifkey="21"
                     /label=SYFP2
     misc_feature    156..1049
                     /vntifkey="21"
                     /label=human\ChAT\promoter\894bp
BASE COUNT     1152 a      1368 c      1381 g      1171 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
      121 actccatcac taggggttcc tgcggccgca cgcgtcccgg gagcaggggg tggggaagga 
      181 ggagcagtgg ctggtggcaa gtctgagcca ggacatagcc gatgacagcc tgtgaatgac 
      241 cggccagact ggggttggac cttcggggca gagcaggcag cccttggaaa gccaggctgg 
      301 taggagcatg ggtgggaggc acaaaacaca gacaaacaga catatataca catatatcct 
      361 ctaggcatct ccgaggagtg atggaggaga gagttggtag gtgggaccac aggaaaccat 
      421 cccacccagc tctgccttct tcaagctcag ggtccctgaa gcctgactag tgtctcccga 
      481 atgggggagg ggggggctct gggcagagca aaaggaggca ttggctgcat gtcttagggc 
      541 ctttaccaac tgaccttgtc cttgctgtcc tctcaaaaga ctgccatgct ccggtgaatc 
      601 ctcaccagga cgccacctct ttcactttgc agttgggggc agcccctacg aacccacagc 
      661 actgggaggc gtgggaacaa gcctgtggag agtgtccctg cactgtgcca gcaagggctt 
      721 tacattctct gcccagcact gcaaggcagc cccaggctcc tccatccttg ggcctggttc 
      781 atccatcttt ctggactctc tgttcctggc aacaaccctg ccagtcccga tacatgggcg 
      841 gtgcttgcct ctgggtaggg gtggtgttgg gtggggagag gggtgaggaa attagctaaa 
      901 cctcagcttt agacttgaga gaatagaggg tgggctggtg gaaaacatga gtatgtgcct 
      961 gtggaggctt aggttcaggg ggatctcaga gggtagcaat tgtgacccac agcctaataa 
     1021 taacagtctt tgccctcttg gcctggctcg gatccagatc tttcgaagct agcgctaccg 
     1081 gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 
     1141 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat 
     1201 gccacctacg gcaagctgac cctgaagctg atctgcacca ccggcaagct gcccgtgccc 
     1261 tggcccaccc tcgtgaccac cctgggctac ggcgtgcagt gcttcgcccg ctaccccgac 
     1321 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc 
     1381 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc 
     1441 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc 
     1501 ctggggcaca agctggagta caactacaac agccacaacg tctatatcac cgccgacaag 
     1561 cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga cggcggcgtg 
     1621 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc 
     1681 gacaaccact acctgagcta ccagtccaag ctgagcaaag accccaacga gaagcgcgat 
     1741 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg 
     1801 tacaagtaag tcgacggcgc gccgcggccg cgaattcgat atcataatca acctctggat 
     1861 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt 
     1921 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc 
     1981 tcctccttgt ataaatcctg gttagttctt gccacggcgg aactcatcgc cgcctgcctt 
     2041 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggc tcgagagatc 
     2101 ttcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt 
     2161 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca 
     2221 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga 
     2281 ggattgggaa gacaatagca ggcatgagat ctcacgtgcg gaccgagcgg ccgcaggaac 
     2341 ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc 
     2401 gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc 
     2461 gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt 
     2521 cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc 
     2581 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc 
     2641 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa 
     2701 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact 
     2761 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt 
     2821 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa 
     2881 ccctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg cctattggtt 
     2941 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac 
     3001 aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg 
     3061 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta 
     3121 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc 
     3181 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat 
     3241 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat 
     3301 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata 
     3361 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct 
     3421 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa 
     3481 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa 
     3541 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt 
     3601 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg 
     3661 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca 
     3721 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa 
     3781 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt 
     3841 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc 
     3901 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa 
     3961 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga 
     4021 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc 
     4081 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga 
     4141 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga 
     4201 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga 
     4261 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat 
     4321 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 
     4381 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 
     4441 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 
     4501 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 
     4561 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 
     4621 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 
     4681 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 
     4741 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 
     4801 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 
     4861 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 
     4921 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 
     4981 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 
     5041 cctggccttt tgctggcctt ttgctcacat gt 
//
