LOCUS       Exported                7068 bp ds-DNA     circular SYN 02-MAR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7068)
  AUTHORS   SnapGene License
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Mar 20, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7068
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          760..4208
                     /organism="Homo sapiens"
                     /chromosome="1"
                     /map="1q32.1"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     primer_bind     136..149
                     /label=MluI-EF1a-SF
     promoter        168..711
                     /label=Ef1a
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
     promoter        198..409
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             422..690
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     primer_bind     525..534
                     /label=MluI-EF1a-SF
     primer_bind     complement(715..732)
                     /label=BsiWI-EF1a-R
     regulatory      718..723
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     misc_feature    724..726
                     /label=NcoI?
     misc_feature    727..758
                     /label=HA
     CDS             727..758
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X11"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384781"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_017172206.2"
                     /translation="YPYDVPDYAG"
     CDS             727..758
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X3"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384765"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_006521284.3"
                     /translation="YPYDVPDYAG"
     CDS             727..758
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X5"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384769"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_006521286.3"
                     /translation="YPYDVPDYAG"
     CDS             727..758
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X10"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384779"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_017172205.2"
                     /translation="YPYDVPDYAG"
     CDS             727..758
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X9"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384777"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_030104537.1"
                     /translation="YPYDVPDYAG"
     CDS             727..758
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X8"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384775"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_017172203.2"
                     /translation="YPYDVPDYAG"
     CDS             727..758
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X4"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384767"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_006521285.3"
                     /translation="YPYDVPDYAG"
     CDS             727..758
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X6"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384771"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_006521287.3"
                     /translation="YPYDVPDYAG"
     CDS             727..758
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X2"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384763"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_030104535.1"
                     /translation="YPYDVPDYAG"
     CDS             727..758
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 
                     3"
                     /label=SH3 and multiple ankyrin repeat domains protein 3
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /db_xref="GI:1690553899"
                     /db_xref="CCDS:CCDS27754.1"
                     /db_xref="GeneID:58234"
                     /protein_id="NP_067398.3"
                     /translation="YPYDVPDYAG"
     mRNA            727..758
                     /product="SH3 and multiple ankyrin repeat domains 3"
                     /label=SH3 and multiple ankyrin repeat domains 3 mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /db_xref="GeneID:58234"
     mRNA            727..758
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X11"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 5 mRNAs, 1 EST, 23 long SRA reads, 
                     2 Proteins, and 70% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 7 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            727..758
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X5"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 4 mRNAs, 2 ESTs, 42 long SRA reads,
                     12 Proteins, and 77% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 8 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            727..758
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X10"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 4 mRNAs, 1 EST, 25 long SRA reads, 
                     2 Proteins, and 71% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 12 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            727..758
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X8"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 6 mRNAs, 1 EST, 26 long SRA reads, 
                     11 Proteins, and 72% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 10 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            727..758
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X4"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 5 mRNAs, 2 ESTs, 42 long SRA reads,
                     12 Proteins, and 77% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 9 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            727..758
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X3"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 4 mRNAs, 4 ESTs, 48 long SRA reads,
                     11 Proteins, and 80% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 8 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            727..758
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X9"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 6 mRNAs, 10 ESTs, 50 long SRA 
                     reads, 12 Proteins, and 77% coverage of the annotated 
                     genomic feature by RNAseq alignments, including 25 samples 
                     with support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            727..758
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X2"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 5 mRNAs, 11 ESTs, 76 long SRA 
                     reads, 13 Proteins, and 81% coverage of the annotated 
                     genomic feature by RNAseq alignments, including 28 samples 
                     with support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            727..758
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X6"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 4 mRNAs, 10 ESTs, 47 long SRA 
                     reads, 11 Proteins, and 77% coverage of the annotated 
                     genomic feature by RNAseq alignments, including 5 samples 
                     with support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            727..758
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X1"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 8 mRNAs, 11 ESTs, 75 long SRA 
                     reads, 17 Proteins, and 81% coverage of the annotated 
                     genomic feature by RNAseq alignments, including 10 samples 
                     with support for all annotated introns"
                     /db_xref="GeneID:58234"
     primer_bind     743..774
                     /label=A15E1-Afl2-F
     primer_bind     743..759
                     /label=Primer 2
     CDS             757..758
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X11"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384781"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_017172206.2"
                     /translation=""
     CDS             757..758
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X3"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384765"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_006521284.3"
                     /translation=""
     CDS             757..758
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X5"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384769"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_006521286.3"
                     /translation=""
     CDS             757..758
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X10"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384779"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_017172205.2"
                     /translation=""
     CDS             757..758
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X9"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384777"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_030104537.1"
                     /translation=""
     CDS             757..758
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X8"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384775"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_017172203.2"
                     /translation=""
     CDS             757..758
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X4"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384767"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_006521285.3"
                     /translation=""
     CDS             757..758
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X6"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384771"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_006521287.3"
                     /translation=""
     CDS             757..758
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X2"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384763"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_030104535.1"
                     /translation=""
     CDS             757..758
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 
                     3"
                     /label=SH3 and multiple ankyrin repeat domains protein 3
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /db_xref="GI:1690553899"
                     /db_xref="CCDS:CCDS27754.1"
                     /db_xref="GeneID:58234"
                     /protein_id="NP_067398.3"
                     /translation=""
     mRNA            757..758
                     /product="SH3 and multiple ankyrin repeat domains 3"
                     /label=SH3 and multiple ankyrin repeat domains 3 mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /db_xref="GeneID:58234"
     mRNA            757..758
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X11"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 5 mRNAs, 1 EST, 23 long SRA reads, 
                     2 Proteins, and 70% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 7 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            757..758
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X5"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 4 mRNAs, 2 ESTs, 42 long SRA reads,
                     12 Proteins, and 77% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 8 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            757..758
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X10"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 4 mRNAs, 1 EST, 25 long SRA reads, 
                     2 Proteins, and 71% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 12 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            757..758
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X8"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 6 mRNAs, 1 EST, 26 long SRA reads, 
                     11 Proteins, and 72% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 10 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            757..758
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X4"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 5 mRNAs, 2 ESTs, 42 long SRA reads,
                     12 Proteins, and 77% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 9 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            757..758
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X3"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 4 mRNAs, 4 ESTs, 48 long SRA reads,
                     11 Proteins, and 80% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 8 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            757..758
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X9"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 6 mRNAs, 10 ESTs, 50 long SRA 
                     reads, 12 Proteins, and 77% coverage of the annotated 
                     genomic feature by RNAseq alignments, including 25 samples 
                     with support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            757..758
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X2"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 5 mRNAs, 11 ESTs, 76 long SRA 
                     reads, 13 Proteins, and 81% coverage of the annotated 
                     genomic feature by RNAseq alignments, including 28 samples 
                     with support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            757..758
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X6"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 4 mRNAs, 10 ESTs, 47 long SRA 
                     reads, 11 Proteins, and 77% coverage of the annotated 
                     genomic feature by RNAseq alignments, including 5 samples 
                     with support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            757..758
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X1"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 8 mRNAs, 11 ESTs, 75 long SRA 
                     reads, 17 Proteins, and 81% coverage of the annotated 
                     genomic feature by RNAseq alignments, including 10 samples 
                     with support for all annotated introns"
                     /db_xref="GeneID:58234"
     misc_feature    758..759
                     /label=Afl II
     CDS             760..4208
                     /codon_start=1
                     /gene="KDM5B"
                     /gene_synonym="CT31; JARID1B; MRT65; PLU-1; PLU1; PPP1R98;
                     PUT1; RBBP2H1A; RBP2-H1"
                     /product="lysine-specific demethylase 5B isoform 2"
                     /EC_number="1.14.11.67"
                     /label=KDM5B
                     /note="isoform 2 is encoded by transcript variant 2;
                     putative DNA/chromatin binding motif; cancer/testis antigen
                     31; jumonji, AT rich interactive domain 1B; 
                     retinoblastoma-binding protein 2, homolog 1A; histone 
                     demethylase JARID1B; jumonji/ARID domain-containing protein
                     1B; retinoblastoma-binding protein 2 homolog 1; protein 
                     phosphatase 1, regulatory subunit 98; lysine (K)-specific 
                     demethylase 5B; [histone H3]-trimethyl-L-lysine(4) 
                     demethylase 5B"
                     /db_xref="GI:57242796"
                     /db_xref="CCDS:CCDS30974.1"
                     /db_xref="GeneID:10765"
                     /db_xref="HGNC:HGNC:18039"
                     /db_xref="MIM:605393"
                     /protein_id="NP_006609.3"
                     /translation="EAATTLHPGPRPALPLGGPGPLGEFLPPPECPVFEPSWEEFADPF
                     AFIHKIRPIAEQTGICKVRPPPDWQPPFACDVDKLHFTPRIQRLNELEAQTRVKLNFLD
                     QIAKYWELQGSTLKIPHVERKILDLFQLNKLVAEEGGFAVVCKDRKWTKIATKMGFAPG
                     KAVGSHIRGHYERILNPYNLFLSGDSLRCLQKPNLTTDTKDKEYKPHDIPQRQSVQPSE
                     TCPPARRAKRMRAEAMNIKIEPEETTEARTHNLRRRMGCPTPKCENEKEMKSSIKQEPI
                     ERKDYIVENEKEKPKSRSKKATNAVDLYVCLLCGSGNDEDRLLLCDGCDDSYHTFCLIP
                     PLHDVPKGDWRCPKCLAQECSKPQEAFGFEQAARDYTLRTFGEMADAFKSDYFNMPVHM
                     VPTELVEKEFWRLVSTIEEDVTVEYGADIASKEFGSGFPVRDGKIKLSPEEEEYLDSGW
                     NLNNMPVMEQSVLAHITADICGMKLPWLYVGMCFSSFCWHIEDHWSYSINYLHWGEPKT
                     WYGVPGYAAEQLENVMKKLAPELFVSQPDLLHQLVTIMNPNTLMTHEVPVYRTNQCAGE
                     FVITFPRAYHSGFNQGFNFAEAVNFCTVDWLPLGRQCVEHYRLLHRYCVFSHDEMICKM
                     ASKADVLDVVVASTVQKDMAIMIEDEKALRETVRKLGVIDSERMDFELLPDDERQCVKC
                     KTTCFMSAISCSCKPGLLVCLHHVKELCSCPPYKYKLRYRYTLDDLYPMMNALKLRAES
                     YNEWALNVNEALEAKINKKKSLVSFKALIEESEMKKFPDNDLLRHLRLVTQDAEKCASV
                     AQQLLNGKRQTRYRSGGGKSQNQLTVNELRQFVTQLYALPCVLSQTPLLKDLLNRVEDF
                     QQHSQKLLSEETPSAAELQDLLDVSFEFDVELPQLAEMRIRLEQARWLEEVQQACLDPS
                     SLTLDDMRRLIDLGVGLAPYSAVEKAMARLQELLTVSEHWDDKAKSLLKARPRHSLNSL
                     ATAVKEIEEIPAYLPNGAALKDSVQRARDWLQDVEGLQAGGRVPVLDTLIELVTRGRSI
                     PVHLNSLPRLETLVAEVQAWKECAVNTFLTENSPYSLLEVLCPRCDIGLLGLKRKQRKL
                     KEPLPNGKKKSTKLESLSDLERALTESKETASAMATLGEARL"
     primer_bind     1462..1478
                     /label=15A-KDM5B-SF
     primer_bind     1575..1591
                     /label=A15E-SF
     primer_bind     complement(3018..3037)
                     /label=A15E1-1R
     primer_bind     3021..3040
                     /label=A15E2-2F
     primer_bind     complement(3482..3498)
                     /label=A15E-SR
     primer_bind     3837..3853
                     /label=15B-KDM5B-SF
     exon            4180..4208
                     /gene="KDM5B"
                     /gene_synonym="CT31; JARID1B; MRT65; PLU-1; PLU1; PPP1R98;
                     PUT1; RBBP2H1A; RBP2-H1"
                     /inference="alignment:same species:2.1.0"
     primer_bind     complement(4194..4226)
                     /label=A15E2-SpeI-R
     misc_feature    4206..4208
                     /label=Feature 47
     intron          4209..4250
                     /label=Splicing donor
                     /note="Splicing donor"
     primer_bind     complement(4209..4226)
                     /label=Primer 6
     misc_feature    4209..4210
                     /label=SpeI?
     primer_bind     complement(4230..4254)
                     /label=R-SD-AgeI
     misc_feature    4255..4287
                     /label=lox JT15
     primer_bind     4261..4302
                     /label=Primer 3
     misc_feature    4267..4274
                     /label=2272
     primer_bind     complement(4305..4319)
                     /label=333A-AgeI-R
     primer_bind     complement(4323..4336)
                     /label=MluI-EF1a-SF
     repeat_region   4332..4472
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4547..5002
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     5137..5159
                     /label=T0056_PGEX-3
     primer_bind     5137..5159
                     /label=PGEX-3
     primer_bind     complement(5249..5268)
                     /label=_pBR322.F
     primer_bind     complement(5249..5268)
                     /label=pBR322.F
     primer_bind     complement(5265..5284)
                     /label=_PTRG-R
     primer_bind     complement(5265..5284)
                     /label=PTRG-R
     promoter        5284..5388
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5389..6249
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="*VFNISVSPLFPFLRHFAFLFLLTQKRW*K*KMLKISWVHEWVTS
                     NWISTAVRSLRVFAPKNVFQ**ALLKFCYVARYYPVLTPGKSNSVAAYTILRMTWLSTH
                     QSQKSILRMA*Q*ENYAVLP*P*VITLRPTYF*QRSEDRRS*PLFCTTWGIM*LALIVG
                     NRS*MKPYQTTSVTPRCL*QWQQRCANY*LANYLL*LPGNN**TGWRRIKLQDHFCARP
                     FRLAGLLLINLEPVSVGLAVSLQHWGQMVSPPVS*LSTRRGVRQLWMNEIDRSLR*VPH
                     *LSIGN"
     primer_bind     complement(5405..5422)
                     /label=T0089_Amp-F
     primer_bind     complement(5405..5422)
                     /label=Amp-F
     primer_bind     complement(5606..5625)
                     /label=T0010_Amp-R
     primer_bind     complement(5606..5625)
                     /label=Amp-R
     primer_bind     6141..6164
                     /label=_PrimerBlaM1
     primer_bind     6141..6164
                     /label=PrimerBlaM1
     primer_bind     complement(6346..6369)
                     /label=_PrimerBlaRV
     primer_bind     complement(6346..6369)
                     /label=PrimerBlaRV
     rep_origin      6420..7008
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtcga ctctagagga tctgcgatcg
      181 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      241 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      301 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      361 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
      421 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
      481 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
      541 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
      601 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
      661 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta ccgtacggcc
      721 accatgtacc cttatgacgt acctgactat gctggatctg aggcggccac cacactgcac
      781 ccaggcccgc gcccggcgct gcccctcggg ggcccgggcc cgctgggcga gttcctgcct
      841 ccacccgagt gcccggtctt cgaacccagc tgggaagagt tcgcggaccc cttcgctttc
      901 atccacaaga tccggcccat agccgagcag actggcatct gtaaggtgcg gccgccgccg
      961 gattggcagc caccatttgc atgtgatgtt gataaacttc attttacgcc acgtatccag
     1021 agactgaatg aattggaggc ccaaactcgt gtaaaattga atttcttgga ccagattgca
     1081 aagtactggg agttacaggg aagtactctg aaaattccac atgtggagag gaagatcttg
     1141 gacttatttc agcttaataa gttagttgca gaagaaggtg gatttgcagt tgtttgcaag
     1201 gatagaaaat ggaccaaaat tgctaccaag atggggtttg ctcctggcaa agcagtgggc
     1261 tcacatatca gagggcatta tgaacgaatt ctcaacccct acaacttatt cctgtccgga
     1321 gacagcctaa ggtgtttgca gaagccaaac ctgaccacag acactaagga caaggagtac
     1381 aaaccccatg atattcccca gaggcagtct gtgcagcctt cggaaacgtg ccccccagcc
     1441 cgacgagcaa aacgcatgag agcagaggcc atgaatatta aaatagaacc cgaggagaca
     1501 acagaagcca gaactcataa tctgagacgt cgaatgggtt gtccaactcc aaaatgtgaa
     1561 aatgagaaag aaatgaagag tagcatcaag caagaaccta ttgagaggaa agattatatt
     1621 gtagaaaatg agaaggaaaa gcccaagagt cgatctaaaa aagccaccaa tgctgtggac
     1681 ctgtatgtct gtcttttatg tggcagtggc aatgatgaag accggctact gttgtgtgat
     1741 ggctgtgatg acagttacca taccttttgc ttgatcccac ctctccatga tgttcccaag
     1801 ggagactgga ggtgtcctaa gtgtttggct caggaatgta gtaagccaca agaagcattt
     1861 ggctttgaac aagcagccag ggactatacc ctccgtactt ttggggaaat ggcagatgcg
     1921 ttcaaatctg attacttcaa catgccagtc catatggtcc ccacagagct tgttgagaaa
     1981 gaattttgga gactagtaag cactattgag gaggatgtca cagtggaata tggagctgac
     2041 attgcctcaa aggaatttgg cagtggcttt cctgtccgag atgggaaaat caaactctca
     2101 cctgaggaag aggagtatct tgatagtggc tggaatttga acaacatgcc agtgatggag
     2161 cagtctgtcc ttgcacatat tactgctgat atatgtggca tgaaacttcc ttggttgtat
     2221 gtgggaatgt gcttttcttc attctgttgg cacattgaag accactggag ctattcaatt
     2281 aactacttgc actggggtga gccaaaaacc tggtatggag tcccagggta tgctgctgag
     2341 cagctagaaa atgtaatgaa gaaactagct ccagaactct ttgtgtccca gccggatctc
     2401 ctccatcagc ttgtgaccat catgaacccc aataccctga tgactcatga agtgcctgtt
     2461 taccgaacta atcagtgtgc tggggagttt gtgattacat ttccaagagc ctaccacagt
     2521 ggttttaacc agggttttaa ttttgctgag gctgttaact tctgcactgt tgattggctg
     2581 ccattaggcc gacagtgtgt ggagcattat cgcttgcttc atcgatattg tgtgttttcc
     2641 catgatgaga tgatctgcaa gatggcttcc aaggctgatg tattagatgt tgtagtggct
     2701 tcaactgttc agaaagacat ggccattatg attgaggatg agaaagcttt aagagaaact
     2761 gtccgtaaat tgggagtgat tgattcggaa agaatggatt ttgagctgtt gccagatgat
     2821 gaacgtcagt gtgtaaaatg caaaactaca tgcttcatgt ctgccatctc ctgttcttgt
     2881 aaacctggcc ttcttgtttg cctgcatcat gtaaaagaat tgtgttcctg tcctccttat
     2941 aaatataaat tgcggtatag gtacacgctg gatgatctct accctatgat gaatgcattg
     3001 aagcttcgag cagaatctta caacgaatgg gccttgaatg tgaatgaagc tttggaggca
     3061 aagatcaaca agaagaaaag ccttgtcagc ttcaaggctt taattgaaga atctgaaatg
     3121 aagaaattcc cagacaatga tcttttgcga caccttcgcc tagtcacaca ggatgcagag
     3181 aagtgtgcct ctgttgcgca gcagttgctt aatggcaaaa ggcaaactag atatcgatct
     3241 ggtggaggga aatcccaaaa tcagttgaca gtgaatgagc tccggcagtt tgtaacacag
     3301 ctgtatgctc ttccatgtgt cctcagtcag acaccattac taaaggatct cttgaatcgt
     3361 gtagaagatt ttcaacagca tagtcagaaa ctactctctg aggaaacgcc tagtgctgcg
     3421 gagctgcagg acttgctaga tgtcagcttt gaatttgatg ttgaacttcc acagcttgct
     3481 gagatgcgta tccgtttgga acaagcccgt tggctagaag aggtgcagca agcttgccta
     3541 gaccccagct cccttacttt agatgatatg agacgtctca tagacctagg ggtagggctg
     3601 gccccgtatt cagcagtgga gaaagctatg gcccggctgc aggaactgct cacagtgtca
     3661 gagcactggg acgacaaagc caagagtctc ctcaaggcca ggccacgaca ttcattgaat
     3721 agccttgcta cggcagtaaa ggaaatcgaa gagatccctg catatctgcc caatggtgcg
     3781 gctctgaaag actcagtgca gagagccaga gactggcttc aggatgtaga gggcctgcag
     3841 gctggaggac gtgtgccagt gttagacaca ctcatagaac ttgttacacg aggccgatct
     3901 atcccggtac atctgaattc tttgccaaga ctggaaaccc tagtagctga ggttcaggct
     3961 tggaaagaat gtgctgttaa tacattcttg actgagaatt ctccatattc tctcttagag
     4021 gtgctgtgtc ctcgatgtga tattggcctt ttgggattga aaaggaagca gagaaagtta
     4081 aaggagccct tgccaaatgg aaagaaaaaa agcaccaaat tagagagtct gagtgacctg
     4141 gagagagctt taactgaaag caaggagact gcttcagcta tggcaactct tggggaagct
     4201 cgcctaaggt aagtatcaag gttacaagac aggtttaagg agaccaataa gctattattc
     4261 gtataggata ctttatacga agttattaag ttatggagac cggtgtggca cgtgcggacc
     4321 gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg
     4381 ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca
     4441 gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg
     4501 catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg
     4561 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     4621 ccttagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     4681 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     4741 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga
     4801 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     4861 ctggaacaac actcaactct atctcgggct attcttttga tttataaggg attttgccga
     4921 tttcggtcta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     4981 aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat
     5041 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     5101 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     5161 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     5221 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     5281 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     5341 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     5401 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     5461 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     5521 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     5581 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     5641 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     5701 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     5761 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     5821 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     5881 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     5941 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     6001 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     6061 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     6121 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     6181 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     6241 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     6301 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     6361 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     6421 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     6481 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     6541 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     6601 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     6661 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6721 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6781 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6841 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6901 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6961 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     7021 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt
//
