LOCUS       Exported                6446 bp ds-DNA     circular SYN 09-MAR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6446)
  AUTHORS   SnapGene License
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Mar 20, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6446
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          748..3586
                     /organism="Homo sapiens"
                     /chromosome="9"
                     /map="9p24.3"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     promoter        157..699
                     /label=Ef1a
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
     promoter        186..397
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             410..678
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      706..711
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     misc_feature    712..714
                     /label=NcoI?
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X1"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 8 mRNAs, 11 ESTs, 75 long SRA 
                     reads, 17 Proteins, and 81% coverage of the annotated 
                     genomic feature by RNAseq alignments, including 10 samples 
                     with support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X6"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 4 mRNAs, 10 ESTs, 47 long SRA 
                     reads, 11 Proteins, and 77% coverage of the annotated 
                     genomic feature by RNAseq alignments, including 5 samples 
                     with support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X2"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 5 mRNAs, 11 ESTs, 76 long SRA 
                     reads, 13 Proteins, and 81% coverage of the annotated 
                     genomic feature by RNAseq alignments, including 28 samples 
                     with support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X9"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 6 mRNAs, 10 ESTs, 50 long SRA 
                     reads, 12 Proteins, and 77% coverage of the annotated 
                     genomic feature by RNAseq alignments, including 25 samples 
                     with support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X3"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 4 mRNAs, 4 ESTs, 48 long SRA reads,
                     11 Proteins, and 80% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 8 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X4"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 5 mRNAs, 2 ESTs, 42 long SRA reads,
                     12 Proteins, and 77% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 9 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X8"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 6 mRNAs, 1 EST, 26 long SRA reads, 
                     11 Proteins, and 72% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 10 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X10"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 4 mRNAs, 1 EST, 25 long SRA reads, 
                     2 Proteins, and 71% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 12 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X5"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 4 mRNAs, 2 ESTs, 42 long SRA reads,
                     12 Proteins, and 77% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 8 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X11"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 5 mRNAs, 1 EST, 23 long SRA reads, 
                     2 Proteins, and 70% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 7 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3"
                     /label=SH3 and multiple ankyrin repeat domains 3 mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /db_xref="GeneID:58234"
     CDS             715..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 
                     3"
                     /label=SH3 and multiple ankyrin repeat domains protein 3
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /db_xref="GI:1690553899"
                     /db_xref="CCDS:CCDS27754.1"
                     /db_xref="GeneID:58234"
                     /protein_id="NP_067398.3"
                     /translation="YPYDVPDYAG"
     CDS             715..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X2"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384763"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_030104535.1"
                     /translation="YPYDVPDYAG"
     CDS             715..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X6"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384771"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_006521287.3"
                     /translation="YPYDVPDYAG"
     CDS             715..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X4"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384767"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_006521285.3"
                     /translation="YPYDVPDYAG"
     CDS             715..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X8"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384775"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_017172203.2"
                     /translation="YPYDVPDYAG"
     CDS             715..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X9"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384777"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_030104537.1"
                     /translation="YPYDVPDYAG"
     CDS             715..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X10"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384779"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_017172205.2"
                     /translation="YPYDVPDYAG"
     CDS             715..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X5"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384769"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_006521286.3"
                     /translation="YPYDVPDYAG"
     CDS             715..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X3"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384765"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_006521284.3"
                     /translation="YPYDVPDYAG"
     CDS             715..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X11"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384781"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_017172206.2"
                     /translation="YPYDVPDYAG"
     misc_feature    715..746
                     /label=HA
     primer_bind     731..762
                     /label=A19E1-Afl2-F
     primer_bind     731..747
                     /label=Primer 2
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X1"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 8 mRNAs, 11 ESTs, 75 long SRA 
                     reads, 17 Proteins, and 81% coverage of the annotated 
                     genomic feature by RNAseq alignments, including 10 samples 
                     with support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X6"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 4 mRNAs, 10 ESTs, 47 long SRA 
                     reads, 11 Proteins, and 77% coverage of the annotated 
                     genomic feature by RNAseq alignments, including 5 samples 
                     with support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X2"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 5 mRNAs, 11 ESTs, 76 long SRA 
                     reads, 13 Proteins, and 81% coverage of the annotated 
                     genomic feature by RNAseq alignments, including 28 samples 
                     with support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X9"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 6 mRNAs, 10 ESTs, 50 long SRA 
                     reads, 12 Proteins, and 77% coverage of the annotated 
                     genomic feature by RNAseq alignments, including 25 samples 
                     with support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X3"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 4 mRNAs, 4 ESTs, 48 long SRA reads,
                     11 Proteins, and 80% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 8 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X4"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 5 mRNAs, 2 ESTs, 42 long SRA reads,
                     12 Proteins, and 77% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 9 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X8"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 6 mRNAs, 1 EST, 26 long SRA reads, 
                     11 Proteins, and 72% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 10 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X10"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 4 mRNAs, 1 EST, 25 long SRA reads, 
                     2 Proteins, and 71% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 12 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X5"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 4 mRNAs, 2 ESTs, 42 long SRA reads,
                     12 Proteins, and 77% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 8 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X11"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 5 mRNAs, 1 EST, 23 long SRA reads, 
                     2 Proteins, and 70% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 7 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3"
                     /label=SH3 and multiple ankyrin repeat domains 3 mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /db_xref="GeneID:58234"
     CDS             745..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 
                     3"
                     /label=SH3 and multiple ankyrin repeat domains protein 3
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /db_xref="GI:1690553899"
                     /db_xref="CCDS:CCDS27754.1"
                     /db_xref="GeneID:58234"
                     /protein_id="NP_067398.3"
                     /translation=""
     CDS             745..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X2"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384763"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_030104535.1"
                     /translation=""
     CDS             745..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X6"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384771"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_006521287.3"
                     /translation=""
     CDS             745..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X4"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384767"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_006521285.3"
                     /translation=""
     CDS             745..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X8"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384775"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_017172203.2"
                     /translation=""
     CDS             745..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X9"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384777"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_030104537.1"
                     /translation=""
     CDS             745..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X10"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384779"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_017172205.2"
                     /translation=""
     CDS             745..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X5"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384769"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_006521286.3"
                     /translation=""
     CDS             745..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X3"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384765"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_006521284.3"
                     /translation=""
     CDS             745..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X11"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384781"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_017172206.2"
                     /translation=""
     misc_feature    746..747
                     /label=Afl II
     CDS             748..3586
                     /codon_start=1
                     /gene="SMARCA2"
                     /gene_synonym="BAF190; BIS; BRM; hBRM; hSNF2a; NCBRS;
                     SNF2; SNF2L2; SNF2LA; Sth1p; SWI2"
                     /product="probable global transcription activator SNF2L2
                     isoform a"
                     /label=SMARCA2
                     /note="isoform a is encoded by transcript variant 1;
                     global transcription activator homologous sequence; 
                     SNF2-alpha; sucrose nonfermenting 2-like protein 2; 
                     SNF2/SWI2-like protein 2; SWI/SNF-related matrix-associated
                     actin-dependent regulator of chromatin a2; brahma homolog; 
                     probable global transcription activator SNF2L2; BAF190B; 
                     protein brahma homolog; BRG1-associated factor 190B; 
                     ATP-dependent helicase SMARCA2; putative global 
                     transcription activator SNF2L2"
                     /db_xref="GI:48255900"
                     /db_xref="CCDS:CCDS34977.1"
                     /db_xref="GeneID:6595"
                     /db_xref="HGNC:HGNC:11098"
                     /db_xref="MIM:600014"
                     /protein_id="NP_003061.3"
                     /translation="STPTDPGAMPHPGPSPGPGPSPGPILGPSPGPGPSPGSVHSMMGP
                     SPGPPSVSHPMPTMGSTDFPQEGMHQMHKPIDGIHDKGIVEDIHCGSMKGTGMRPPHPG
                     MGPPQSPMDQHSQGYMSPHPSPLGAPEHVSSPMSGGGPTPPQMPPSQPGALIPGDPQAM
                     SQPNRGPSPFSPVQLHQLRAQILAYKMLARGQPLPETLQLAVQGKRTLPGLQQQQQQKQ
                     SRISPIQKPQGLDPVEILQEREYRLQARIAHRIQELENLPGSLPPDLRTKATVELKALR
                     LLNFQRQLRQEVVACMRRDTTLETALNSKAYKRSKRQTLREARMTEKLEKQQKIEQERK
                     RRQKHQEYLNSILQHAKDFKEYHRSVAGKIQKLSKAVATWHANTEREQKKETERIEKER
                     MRRLMAEDEEGYRKLIDQKKDRRLAYLLQQTDEYVANLTNLVWEHKQAQAAKEKKKRRR
                     RKKKAEENAEGGESALGPDGEPIDESSQMSDLPVKVTHTETGKVLFGPEAPKASQLDAW
                     LEMNPGYEVAPRSDSEESDSDYEEEDEEEESSRQETEEKILLDPNSEEVSEKDAKQIIE
                     TAKQDVDDEYSMQYSARGSQSYYTVAHAISERVEKQSALLINGTLKHYQLQGLEWMVSL
                     YNNNLNGILADEMGLGKTIQTIALITYLMEHKRLNGPYLIIVPLSTLSNWTYEFDKWAP
                     SVVKISYKGTPAMRRSLVPQLRSGKFNVLLTTYEYIIKDKHILAKIRWKYMIVDEGHRM
                     KNHHCKLTQVLNTHYVAPRRILLTGTPLQNKLPELWALLNFLLPTIFKSCSTFEQWFNA
                     PFAMTGERVDLNEEETILIIRRLHKVLRPFLLRRLKKEVESQLPEKVEYVIKCDMSALQ
                     KILYRHMQAKGILLTDGSEKDKKGKGGAKTLMNTIMQLRKICNHPYMFQHIEESFAEHL
                     GYSNGVINGAELYRAS"
     primer_bind     1507..1523
                     /label=A19E-SF
     primer_bind     complement(2866..2882)
                     /label=A19E-SR
     primer_bind     complement(2992..3010)
                     /label=A19E1-1R
     primer_bind     2994..3012
                     /label=A19E2-2F
     exon            3564..3586
                     /gene="SMARCA2"
                     /gene_synonym="BAF190; BIS; BRM; hBRM; hSNF2a; NCBRS;
                     SNF2; SNF2L2; SNF2LA; Sth1p; SWI2"
                     /inference="alignment:same species:2.1.0"
     primer_bind     complement(3569..3604)
                     /label=A19E2-SpeI-R
     misc_feature    3584..3586
                     /label=Feature 69
     intron          3587..3628
                     /label=Splicing donor
                     /note="Splicing donor"
     primer_bind     complement(3587..3604)
                     /label=Primer 6
     misc_feature    3587..3588
                     /label=SpeI?
     primer_bind     complement(3608..3632)
                     /label=R-SD-AgeI
     misc_feature    3633..3665
                     /label=lox JT15
     primer_bind     3639..3680
                     /label=Primer 3
     misc_feature    3645..3652
                     /label=2272
     primer_bind     complement(3683..3697)
                     /label=333A-AgeI-R
     repeat_region   3710..3850
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      3925..4380
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     4515..4537
                     /label=T0056_PGEX-3
     primer_bind     4515..4537
                     /label=PGEX-3
     primer_bind     complement(4627..4646)
                     /label=_pBR322.F
     primer_bind     complement(4627..4646)
                     /label=pBR322.F
     primer_bind     complement(4643..4662)
                     /label=_PTRG-R
     primer_bind     complement(4643..4662)
                     /label=PTRG-R
     promoter        4662..4766
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4767..5627
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="*VFNISVSPLFPFLRHFAFLFLLTQKRW*K*KMLKISWVHEWVTS
                     NWISTAVRSLRVFAPKNVFQ**ALLKFCYVARYYPVLTPGKSNSVAAYTILRMTWLSTH
                     QSQKSILRMA*Q*ENYAVLP*P*VITLRPTYF*QRSEDRRS*PLFCTTWGIM*LALIVG
                     NRS*MKPYQTTSVTPRCL*QWQQRCANY*LANYLL*LPGNN**TGWRRIKLQDHFCARP
                     FRLAGLLLINLEPVSVGLAVSLQHWGQMVSPPVS*LSTRRGVRQLWMNEIDRSLR*VPH
                     *LSIGN"
     primer_bind     complement(4783..4800)
                     /label=T0089_Amp-F
     primer_bind     complement(4783..4800)
                     /label=Amp-F
     primer_bind     complement(4984..5003)
                     /label=T0010_Amp-R
     primer_bind     complement(4984..5003)
                     /label=Amp-R
     primer_bind     5519..5542
                     /label=_PrimerBlaM1
     primer_bind     5519..5542
                     /label=PrimerBlaM1
     primer_bind     complement(5724..5747)
                     /label=_PrimerBlaRV
     primer_bind     complement(5724..5747)
                     /label=PrimerBlaRV
     rep_origin      5798..6386
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtggatc tgcgatcgct ccggtgcccg
      181 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
      241 ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
      301 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
      361 cgttcttttt cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat
      421 ctctccttca cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt
      481 tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa
      541 gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg
      601 gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt
      661 tctgcgccgt tacagatcca agctgtgacc ggcgcctacc gtacggccac catgtaccct
      721 tatgacgtac ctgactatgc tggatcttcc acgcccacag accctggtgc gatgccccac
      781 ccagggcctt cgccggggcc tgggccttcc cctgggccaa ttcttgggcc tagtccagga
      841 ccaggaccat ccccaggttc cgtccacagc atgatggggc caagtcctgg acctccaagt
      901 gtctcccatc ctatgccgac gatggggtcc acagacttcc cacaggaagg catgcatcaa
      961 atgcataagc ccatcgatgg tatacatgac aaggggattg tagaagacat ccattgtgga
     1021 tccatgaagg gcactggtat gcgaccacct cacccaggca tgggccctcc ccagagtcca
     1081 atggatcaac acagccaagg ttatatgtca ccacacccat ctccattagg agccccagag
     1141 cacgtctcca gccctatgtc tggaggaggc ccaactccac ctcagatgcc accaagccag
     1201 ccgggggccc tcatcccagg tgatccgcag gccatgagcc agcccaacag aggtccctca
     1261 cctttcagtc ctgtccagct gcatcagctt cgagctcaga ttttagctta taaaatgctg
     1321 gcccgaggcc agcccctccc cgaaacgctg cagcttgcag tccaggggaa aaggacgttg
     1381 cctggcttgc agcaacaaca gcagcagaag cagagccgca tcagccccat ccagaaaccg
     1441 caaggcctgg accccgtgga aattctgcaa gagcgggaat acagacttca ggcccgcata
     1501 gctcatagga tacaagaact ggaaaatctg cctggctctt tgccaccaga tttaagaacc
     1561 aaagcaaccg tggaactaaa agcacttcgg ttactcaatt tccagcgtca gctgagacag
     1621 gaggtggtgg cctgcatgcg cagggacacg accctggaga cggctctcaa ctccaaagca
     1681 tacaaacgga gcaagcgcca gactctgaga gaagctcgca tgaccgagaa gctggagaag
     1741 cagcagaaga ttgagcagga gaggaaacgc cgtcagaaac accaggaata cctgaacagt
     1801 attttgcaac atgcaaaaga ttttaaggaa tatcatcggt ctgtggccgg aaagatccag
     1861 aagctctcca aagcagtggc aacttggcat gccaacactg aaagagagca gaagaaggag
     1921 acagagcgga ttgaaaagga gagaatgcgg cgactgatgg ctgaagatga ggagggttat
     1981 agaaaactga ttgatcaaaa gaaagacagg cgtttagctt accttttgca gcagaccgat
     2041 gagtatgtag ccaatctgac caatctggtt tgggagcaca agcaagccca ggcagccaaa
     2101 gagaagaaga agaggaggag gaggaagaag aaggctgagg agaatgcaga gggtggggag
     2161 tctgccctgg gaccggatgg agagcccata gatgagagca gccagatgag tgacctccct
     2221 gtcaaagtga ctcacacaga aaccggcaag gttctgttcg gaccagaagc acccaaagca
     2281 agtcagctgg acgcctggct ggaaatgaat cctggttatg aagttgcccc tagatctgac
     2341 agtgaagaga gtgattctga ttatgaggaa gaggatgagg aagaagagtc cagtaggcag
     2401 gaaaccgaag agaaaatact cctggatcca aatagcgaag aagtttctga gaaggatgct
     2461 aagcagatca ttgagacagc taagcaagac gtggatgatg aatacagcat gcagtacagt
     2521 gccaggggct cccagtccta ctacaccgtg gctcatgcca tctcggagag ggtggagaaa
     2581 cagtctgccc tcctaattaa tgggacccta aagcattacc agctccaggg cctggaatgg
     2641 atggtttccc tgtataataa caacttgaac ggaatcttag ccgatgaaat ggggcttgga
     2701 aagaccatac agaccattgc actcatcact tatctgatgg agcacaaaag actcaatggc
     2761 ccctatctca tcattgttcc cctttcgact ctatctaact ggacatatga atttgacaaa
     2821 tgggctcctt ctgtggtgaa gatttcttac aagggtactc ctgccatgcg tcgctccctt
     2881 gtcccccagc tacggagtgg caaattcaat gtcctcttga ctacttatga gtatattata
     2941 aaagacaagc acattcttgc aaagattcgg tggaaataca tgatagtgga cgaaggccac
     3001 cgaatgaaga atcaccactg caagctgact caggtcttga acactcacta tgtggccccc
     3061 agaaggatcc tcttgactgg gaccccgctg cagaataagc tccctgaact ctgggccctc
     3121 ctcaacttcc tcctcccaac aatttttaag agctgcagca catttgaaca atggttcaat
     3181 gctccatttg ccatgactgg tgaaagggtg gacttaaatg aagaagaaac tatattgatc
     3241 atcaggcgtc tacataaggt gttaagacca tttttactaa ggagactgaa gaaagaagtt
     3301 gaatcccagc ttcccgaaaa agtggaatat gtgatcaagt gtgacatgtc agctctgcag
     3361 aagattctgt atcgccatat gcaagccaag gggatccttc tcacagatgg ttctgagaaa
     3421 gataagaagg ggaaaggagg tgctaagaca cttatgaaca ctattatgca gttgagaaaa
     3481 atctgcaacc acccatatat gtttcagcac attgaggaat cctttgctga acacctaggc
     3541 tattcaaatg gggtcatcaa tggggctgaa ctgtatcggg cctcaggtaa gtatcaaggt
     3601 tacaagacag gtttaaggag accaataagc tattattcgt ataggatact ttatacgaag
     3661 ttattaagtt atggagaccg gtgtggcacg tgcggaccga gcggccgcag gaacccctag
     3721 tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa
     3781 aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct
     3841 gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     3901 gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     3961 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc
     4021 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     4081 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt
     4141 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     4201 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaactctat
     4261 ctcgggctat tcttttgatt tataagggat tttgccgatt tcggtctatt ggttaaaaaa
     4321 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt
     4381 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     4441 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     4501 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     4561 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     4621 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     4681 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     4741 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     4801 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     4861 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     4921 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     4981 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     5041 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     5101 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     5161 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     5221 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     5281 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     5341 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     5401 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     5461 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     5521 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     5581 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     5641 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     5701 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     5761 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     5821 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     5881 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     5941 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     6001 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     6061 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     6121 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     6181 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     6241 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     6301 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     6361 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     6421 cttttgctgg ccttttgctc acatgt
//
