LOCUS       Exported                7208 bp ds-DNA     circular SYN 09-MAR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7208)
  AUTHORS   SnapGene License
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Mar 20, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7208
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          748..4348
                     /organism="Homo sapiens"
                     /chromosome="15"
                     /map="15q26.1"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     promoter        157..699
                     /label=Ef1a
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
     promoter        186..397
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             410..678
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      706..711
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     misc_feature    712..714
                     /label=NcoI?
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X1"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 8 mRNAs, 11 ESTs, 75 long SRA 
                     reads, 17 Proteins, and 81% coverage of the annotated 
                     genomic feature by RNAseq alignments, including 10 samples 
                     with support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X6"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 4 mRNAs, 10 ESTs, 47 long SRA 
                     reads, 11 Proteins, and 77% coverage of the annotated 
                     genomic feature by RNAseq alignments, including 5 samples 
                     with support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X2"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 5 mRNAs, 11 ESTs, 76 long SRA 
                     reads, 13 Proteins, and 81% coverage of the annotated 
                     genomic feature by RNAseq alignments, including 28 samples 
                     with support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X9"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 6 mRNAs, 10 ESTs, 50 long SRA 
                     reads, 12 Proteins, and 77% coverage of the annotated 
                     genomic feature by RNAseq alignments, including 25 samples 
                     with support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X3"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 4 mRNAs, 4 ESTs, 48 long SRA reads,
                     11 Proteins, and 80% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 8 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X4"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 5 mRNAs, 2 ESTs, 42 long SRA reads,
                     12 Proteins, and 77% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 9 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X8"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 6 mRNAs, 1 EST, 26 long SRA reads, 
                     11 Proteins, and 72% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 10 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X10"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 4 mRNAs, 1 EST, 25 long SRA reads, 
                     2 Proteins, and 71% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 12 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X5"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 4 mRNAs, 2 ESTs, 42 long SRA reads,
                     12 Proteins, and 77% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 8 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X11"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 5 mRNAs, 1 EST, 23 long SRA reads, 
                     2 Proteins, and 70% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 7 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3"
                     /label=SH3 and multiple ankyrin repeat domains 3 mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /db_xref="GeneID:58234"
     CDS             715..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 
                     3"
                     /label=SH3 and multiple ankyrin repeat domains protein 3
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /db_xref="GI:1690553899"
                     /db_xref="CCDS:CCDS27754.1"
                     /db_xref="GeneID:58234"
                     /protein_id="NP_067398.3"
                     /translation="YPYDVPDYAG"
     CDS             715..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X2"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384763"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_030104535.1"
                     /translation="YPYDVPDYAG"
     CDS             715..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X6"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384771"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_006521287.3"
                     /translation="YPYDVPDYAG"
     CDS             715..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X4"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384767"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_006521285.3"
                     /translation="YPYDVPDYAG"
     CDS             715..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X8"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384775"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_017172203.2"
                     /translation="YPYDVPDYAG"
     CDS             715..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X9"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384777"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_030104537.1"
                     /translation="YPYDVPDYAG"
     CDS             715..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X10"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384779"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_017172205.2"
                     /translation="YPYDVPDYAG"
     CDS             715..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X5"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384769"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_006521286.3"
                     /translation="YPYDVPDYAG"
     CDS             715..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X3"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384765"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_006521284.3"
                     /translation="YPYDVPDYAG"
     CDS             715..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X11"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384781"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_017172206.2"
                     /translation="YPYDVPDYAG"
     misc_feature    715..746
                     /label=HA
     primer_bind     731..770
                     /label=A33E1-Afl2-F
     primer_bind     731..747
                     /label=Primer 2
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X1"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 8 mRNAs, 11 ESTs, 75 long SRA 
                     reads, 17 Proteins, and 81% coverage of the annotated 
                     genomic feature by RNAseq alignments, including 10 samples 
                     with support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X6"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 4 mRNAs, 10 ESTs, 47 long SRA 
                     reads, 11 Proteins, and 77% coverage of the annotated 
                     genomic feature by RNAseq alignments, including 5 samples 
                     with support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X2"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 5 mRNAs, 11 ESTs, 76 long SRA 
                     reads, 13 Proteins, and 81% coverage of the annotated 
                     genomic feature by RNAseq alignments, including 28 samples 
                     with support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X9"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 6 mRNAs, 10 ESTs, 50 long SRA 
                     reads, 12 Proteins, and 77% coverage of the annotated 
                     genomic feature by RNAseq alignments, including 25 samples 
                     with support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X3"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 4 mRNAs, 4 ESTs, 48 long SRA reads,
                     11 Proteins, and 80% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 8 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X4"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 5 mRNAs, 2 ESTs, 42 long SRA reads,
                     12 Proteins, and 77% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 9 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X8"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 6 mRNAs, 1 EST, 26 long SRA reads, 
                     11 Proteins, and 72% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 10 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X10"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 4 mRNAs, 1 EST, 25 long SRA reads, 
                     2 Proteins, and 71% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 12 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X5"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 4 mRNAs, 2 ESTs, 42 long SRA reads,
                     12 Proteins, and 77% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 8 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X11"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 5 mRNAs, 1 EST, 23 long SRA reads, 
                     2 Proteins, and 70% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 7 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3"
                     /label=SH3 and multiple ankyrin repeat domains 3 mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /db_xref="GeneID:58234"
     CDS             745..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 
                     3"
                     /label=SH3 and multiple ankyrin repeat domains protein 3
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /db_xref="GI:1690553899"
                     /db_xref="CCDS:CCDS27754.1"
                     /db_xref="GeneID:58234"
                     /protein_id="NP_067398.3"
                     /translation=""
     CDS             745..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X2"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384763"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_030104535.1"
                     /translation=""
     CDS             745..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X6"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384771"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_006521287.3"
                     /translation=""
     CDS             745..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X4"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384767"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_006521285.3"
                     /translation=""
     CDS             745..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X8"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384775"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_017172203.2"
                     /translation=""
     CDS             745..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X9"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384777"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_030104537.1"
                     /translation=""
     CDS             745..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X10"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384779"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_017172205.2"
                     /translation=""
     CDS             745..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X5"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384769"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_006521286.3"
                     /translation=""
     CDS             745..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X3"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384765"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_006521284.3"
                     /translation=""
     CDS             745..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X11"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384781"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_017172206.2"
                     /translation=""
     misc_feature    746..747
                     /label=Afl II
     CDS             748..4348
                     /codon_start=1
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /product="chromodomain-helicase-DNA-binding protein 2
                     isoform 1"
                     /EC_number="3.6.4.12"
                     /label=CHD2
                     /note="isoform 1 is encoded by transcript variant 1;
                     CHD-2; ATP-dependent helicase CHD2"
                     /db_xref="GI:118421089"
                     /db_xref="CCDS:CCDS10374.2"
                     /db_xref="GeneID:1106"
                     /db_xref="HGNC:HGNC:1917"
                     /db_xref="MIM:602119"
                     /protein_id="NP_001262.3"
                     /translation="RNKDKSQEEDSSLHSNASSHSASEEASGSDSGSQSESEQGSDPGS
                     GHGSESNSSSESSESQSESESESAGSKSQPVLPEAKEKPASKKERIADVKKMWEEYPDV
                     YGVRRSNRSRQEPSRFNIKEEASSGSESGSPKRRGQRQLKKQEKWKQEPSEDEQEQGTS
                     AESEPEQKKVKARRPVPRRTVPKPRVKKQPKTQRGKRKKQDSSDEDDDDDEAPKRQTRR
                     RAAKNVSYKEDDDFETDSDDLIEMTGEGVDEQQDNSETIEKVLDSRLGKKGATGASTTV
                     YAIEANGDPSGDFDTEKDEGEIQYLIKWKGWSYIHSTWESEESLQQQKVKGLKKLENFK
                     KKEDEIKQWLGKVSPEDVEYFNCQQELASELNKQYQIVERVIAVKTSKSTLGQTDFPAH
                     SRKPAPSNEPEYLCKWMGLPYSECSWEDEALIGKKFQNCIDSFHSRNNSKTIPTRECKA
                     LKQRPRFVALKKQPAYLGGENLELRDYQLEGLNWLAHSWCKNNSVILADEMGLGKTIQT
                     ISFLSYLFHQHQLYGPFLIVVPLSTLTSWQREFEIWAPEINVVVYIGDLMNRNTIREYE
                     WIHSQTKRLKFNALITTYEILLKDKTVLGSINWAFLGVDEAHRLKNDDSLLYKTLIDFK
                     SNHRLLITGTPLQNSLKELWSLLHFIMPEKFEFWEDFEEDHGKGRENGYQSLHKVLEPF
                     LLRRVKKDVEKSLPAKVEQILRVEMSALQKQYYKWILTRNYKALAKGTRGSTSGFLNIV
                     MELKKCCNHCYLIKPPEENERENGQEILLSLIRSSGKLILLDKLLTRLRERGNRVLIFS
                     QMVRMLDILAEYLTIKHYPFQRLDGSIKGEIRKQALDHFNADGSEDFCFLLSTRAGGLG
                     INLASADTVVIFDSDWNPQNDLQAQARAHRIGQKKQVNIYRLVTKGTVEEEIIERAKKK
                     MVLDHLVIQRMDTTGRTILENNSGRSNSNPFNKEELTAILKFGAEDLFKELEGEESEPQ
                     EMDIDEILRLAETRENEVSTSATDELLSQFKVANFATMEDEEELEERPHKDWDEIIPEE
                     QRKKVEEEERQKELEEIYMLPRIRSSTKKAQTNDSDSDTESKRQAQRSSASESETEDSD
                     DDKKPKRRGRPRSVRKDLVEGFTDAEIRRFIKAYKKFGLPLERLECIARDAELVDKSVA
                     DLKRLGELIHNSCVSAMQEYEEQLKENASEGKGP"
     primer_bind     1538..1555
                     /label=A33E-SF1
     primer_bind     complement(1778..1794)
                     /label=33A-CHD2-SF
     primer_bind     2179..2196
                     /label=A33E-SF2
     primer_bind     complement(2560..2579)
                     /label=A33E1-1R
     primer_bind     2563..2583
                     /label=A33E2-2F
     primer_bind     3186..3202
                     /label=33B-CHD2-SF
     primer_bind     complement(3599..3616)
                     /label=A33E-SR1
     primer_bind     complement(4331..4366)
                     /label=A33E2-SpeI-R
     exon            4337..4348
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /inference="alignment:same species:2.1.0"
     misc_feature    4346..4348
                     /label=Feature 67
     intron          4349..4390
                     /label=Splicing donor
                     /note="Splicing donor"
     primer_bind     complement(4349..4366)
                     /label=Primer 6
     misc_feature    4349..4350
                     /label=SpeI?
     primer_bind     complement(4370..4394)
                     /label=R-SD-AgeI
     misc_feature    4395..4427
                     /label=lox JT15
     primer_bind     4401..4442
                     /label=Primer 3
     misc_feature    4407..4414
                     /label=2272
     primer_bind     complement(4445..4459)
                     /label=333A-AgeI-R
     repeat_region   4472..4612
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4687..5142
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     5277..5299
                     /label=T0056_PGEX-3
     primer_bind     5277..5299
                     /label=PGEX-3
     primer_bind     complement(5389..5408)
                     /label=_pBR322.F
     primer_bind     complement(5389..5408)
                     /label=pBR322.F
     primer_bind     complement(5405..5424)
                     /label=_PTRG-R
     primer_bind     complement(5405..5424)
                     /label=PTRG-R
     promoter        5424..5528
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5529..6389
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="*VFNISVSPLFPFLRHFAFLFLLTQKRW*K*KMLKISWVHEWVTS
                     NWISTAVRSLRVFAPKNVFQ**ALLKFCYVARYYPVLTPGKSNSVAAYTILRMTWLSTH
                     QSQKSILRMA*Q*ENYAVLP*P*VITLRPTYF*QRSEDRRS*PLFCTTWGIM*LALIVG
                     NRS*MKPYQTTSVTPRCL*QWQQRCANY*LANYLL*LPGNN**TGWRRIKLQDHFCARP
                     FRLAGLLLINLEPVSVGLAVSLQHWGQMVSPPVS*LSTRRGVRQLWMNEIDRSLR*VPH
                     *LSIGN"
     primer_bind     complement(5545..5562)
                     /label=T0089_Amp-F
     primer_bind     complement(5545..5562)
                     /label=Amp-F
     primer_bind     complement(5746..5765)
                     /label=T0010_Amp-R
     primer_bind     complement(5746..5765)
                     /label=Amp-R
     primer_bind     6281..6304
                     /label=_PrimerBlaM1
     primer_bind     6281..6304
                     /label=PrimerBlaM1
     primer_bind     complement(6486..6509)
                     /label=_PrimerBlaRV
     primer_bind     complement(6486..6509)
                     /label=PrimerBlaRV
     rep_origin      6560..7148
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtggatc tgcgatcgct ccggtgcccg
      181 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
      241 ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
      301 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
      361 cgttcttttt cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat
      421 ctctccttca cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt
      481 tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa
      541 gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg
      601 gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt
      661 tctgcgccgt tacagatcca agctgtgacc ggcgcctacc gtacggccac catgtaccct
      721 tatgacgtac ctgactatgc tggatctaga aataaggaca aaagccaaga ggaggacagt
      781 tcgctacaca gcaatgcatc gagtcactca gcctctgaag aagcttcggg ttcagactca
      841 ggcagtcagt cggaaagtga gcagggaagt gatccaggaa gtggacatgg cagcgagtcg
      901 aacagcagct ctgaatcttc tgagagtcag tcggaatctg agagcgaatc agcaggttcc
      961 aaatcccagc cagtcctccc agaagccaaa gagaagccag cctctaagaa ggaacggata
     1021 gctgatgtga agaagatgtg ggaagaatat cctgatgttt atggggtcag gcggtcaaac
     1081 cgaagcagac aagaaccatc gcgatttaat attaaggaag aggcaagtag cgggtctgag
     1141 agtgggagcc caaaaagaag aggccagagg cagctgaaaa aacaagaaaa atggaaacag
     1201 gaaccctcag aagatgaaca ggaacaaggc accagtgcag agagtgagcc agaacaaaaa
     1261 aaagtaaaag ccagaagacc tgtccccaga agaacagtgc ccaaacctcg tgttaaaaag
     1321 cagccgaaga ctcagcgtgg aaagagaaaa aagcaagatt cttctgatga ggatgatgat
     1381 gatgacgaag ctcccaaaag gcagactcgt cgaagagcgg ctaaaaacgt tagttacaaa
     1441 gaagatgatg actttgagac tgactcagat gatctcattg aaatgactgg agaaggagtt
     1501 gatgaacagc aagataatag tgaaactatt gaaaaggtct tagattcaag actgggaaag
     1561 aaaggagcca ctggagcatc tactactgta tatgcgattg aagctaatgg cgaccctagt
     1621 ggtgactttg acactgaaaa ggatgaaggt gaaatccagt acctcatcaa gtggaagggt
     1681 tggtcttaca tccacagcac atgggagagt gaagaatcct tacagcaaca gaaagtgaag
     1741 ggcctaaaaa aactagagaa cttcaagaaa aaagaggacg aaatcaaaca atggttaggg
     1801 aaagtttctc ctgaagatgt agaatatttc aattgccaac aggagctggc ttcagagttg
     1861 aataaacagt atcagatagt agaaagagta atagctgtga agacaagtaa atctacattg
     1921 ggtcaaacag attttccagc tcatagtcgg aagccggcac cctcaaatga gcccgaatat
     1981 ctatgtaaat ggatgggact cccctattca gagtgtagct gggaagatga agccctcatt
     2041 ggaaagaaat tccagaattg cattgacagc ttccacagta ggaacaactc aaaaaccatc
     2101 ccaacaagag aatgcaaggc cctgaagcag agaccacgat ttgtagcttt aaagaaacaa
     2161 cctgcatatt taggagggga gaatctggaa cttcgagatt atcagctaga aggtctaaac
     2221 tggctagctc attcctggtg caaaaataat agtgtaatcc ttgctgatga aatgggccta
     2281 ggaaagacca tccagaccat atcattcctc tcctacctgt tccaccaaca ccagctgtat
     2341 ggcccctttc ttatagtcgt ccctttatcc accctcacct catggcagag agagtttgaa
     2401 atctgggcac cagagattaa cgtagtggtt tacataggtg acctgatgaa cagaaatacg
     2461 atacgggaat atgaatggat tcattcccaa accaaaagat tgaagttcaa cgcacttata
     2521 acaacatatg agatcctctt gaaagataag actgtgctgg gcagtattaa ctgggccttt
     2581 ctgggagtgg atgaagccca tcggttgaag aatgatgact ctttattgta taaaactctg
     2641 attgatttca agtccaacca taggctcctg attacgggga cccctcttca gaattccctc
     2701 aaagagctct ggtccttgct gcactttatt atgccggaga agtttgaatt ttgggaagat
     2761 tttgaagaag accatgggaa ggggagagag aatggctacc agagtcttca taaggtgcta
     2821 gagcctttcc ttctccggag agtcaaaaaa gatgtggaga aatcccttcc tgctaaagtg
     2881 gaacagattc tcagggtgga gatgtcagcc cttcagaaac agtattacaa gtggattctg
     2941 accaggaatt acaaggctct tgccaaagga acaagaggca gcacatctgg ttttcttaat
     3001 attgtgatgg aactgaaaaa atgttgcaac cactgctatc tgattaaacc ccctgaagaa
     3061 aatgaaaggg aaaatggaca ggagattctt ctgtccctca taaggagcag tgggaagttg
     3121 attttattag acaaactgtt gacaagactt cgagaaaggg ggaatcgagt gcttatcttc
     3181 tctcagatgg tgagaatgtt ggatatcctg gctgaatacc taactattaa acactatcct
     3241 ttccagcgtc tggatggttc catcaaggga gaaatccgaa aacaggcact ggaccacttc
     3301 aatgcagatg ggtctgagga cttctgtttc ctgctctcga caagggctgg tggcctggga
     3361 atcaatttgg cttcagcgga cacagtcgtc atctttgact ctgactggaa cccccagaat
     3421 gacttgcagg cacaagcccg agcgcataga attggtcaga agaagcaggt aaatatttac
     3481 cgcttagtta caaaggggac tgtggaggag gagatcatag aacgggccaa aaagaagatg
     3541 gtattagatc atctggtgat tcagcgcatg gacaccactg gccggacgat cctggaaaac
     3601 aactcaggaa ggtccaactc aaatcctttt aataaagaag agctgacagc tattttgaaa
     3661 tttggagcag aggatctctt caaagaactg gaaggggagg aatcagaacc tcaggaaatg
     3721 gatatagatg aaattttgcg gttggctgaa acgagagaga atgaagtgtc aacaagtgca
     3781 acagatgaac ttctatcaca gtttaaggtt gccaactttg caacaatgga agatgaagaa
     3841 gagctagaag agcgtcctca caaggactgg gatgagatca ttccagagga acaaaggaaa
     3901 aaagtagagg aggaagagcg gcagaaggag ctagaagaaa tttatatgct gcctcgaatt
     3961 cggagttcca ctaaaaaggc tcagacaaat gacagtgact ctgacactga gtctaagagg
     4021 caggcccaga gatcctctgc ttctgagagt gaaacggaag actctgatga tgacaagaag
     4081 ccaaagcgca gagggcgtcc gaggagtgtg cggaaggacc tcgtggaggg atttactgat
     4141 gcagagatcc gaaggttcat caaggcttat aagaagtttg gtctccctct tgaacggctg
     4201 gagtgcatag cacgtgatgc tgagctggta gataagtcgg tggcagatct gaagcgcctg
     4261 ggtgaactga tccacaacag ctgtgtgtca gcaatgcagg aatacgaaga gcagctgaaa
     4321 gaaaatgcca gcgagggaaa aggaccaggt aagtatcaag gttacaagac aggtttaagg
     4381 agaccaataa gctattattc gtataggata ctttatacga agttattaag ttatggagac
     4441 cggtgtggca cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc
     4501 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg
     4561 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga
     4621 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc
     4681 atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     4741 gaccgctaca cttgccagcg ccttagcgcc cgctcctttc gctttcttcc cttcctttct
     4801 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     4861 atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag
     4921 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     4981 tagtggactc ttgttccaaa ctggaacaac actcaactct atctcgggct attcttttga
     5041 tttataaggg attttgccga tttcggtcta ttggttaaaa aatgagctga tttaacaaaa
     5101 atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac
     5161 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     5221 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     5281 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     5341 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     5401 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     5461 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     5521 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     5581 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     5641 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     5701 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     5761 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     5821 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     5881 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     5941 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     6001 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     6061 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     6121 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     6181 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     6241 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     6301 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     6361 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     6421 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     6481 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     6541 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     6601 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     6661 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     6721 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     6781 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     6841 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     6901 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     6961 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     7021 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     7081 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     7141 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     7201 tcacatgt
//
