LOCUS       Exported                6640 bp ds-DNA     circular SYN 16-MAR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6640)
  AUTHORS   SnapGene License
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Mar 20, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6640
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1493..3341
                     /organism="Homo sapiens"
                     /chromosome="X"
                     /map="Xp11.22"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     primer_bind     141..190
                     /label=F-D1
     primer_bind     147..194
                     /label=F-Mlu-D2
     primer_bind     147..161
                     /label=3'-pcalm-ampF
     primer_bind     150..168
                     /label=P-Calm1-F
     primer_bind     151..200
                     /label=F-D2
     misc_feature    156..276
                     /label=P Calm1
     misc_feature    156..165
                     /label=D1
     primer_bind     161..210
                     /label=F-D3
     primer_bind     complement(161..185)
                     /label=R-D4
     misc_feature    166..175
                     /label=D2
     primer_bind     171..220
                     /label=F-D4
     primer_bind     complement(171..195)
                     /label=R-D5
     misc_feature    176..185
                     /label=D3
     primer_bind     181..230
                     /label=F-D5
     primer_bind     complement(181..205)
                     /label=R-D6
     misc_feature    186..195
                     /label=D4
     primer_bind     191..240
                     /label=F-D6
     primer_bind     complement(191..215)
                     /label=r-d7
     misc_feature    196..205
                     /label=D5
     primer_bind     200..250
                     /label=F-D7
     primer_bind     complement(201..225)
                     /label=R-D8
     misc_feature    206..215
                     /label=D6
     primer_bind     211..260
                     /label=F-D8
     primer_bind     complement(215..236)
                     /label=R-D9
     misc_feature    216..225
                     /label=D7
     primer_bind     222..271
                     /label=F-D9
     primer_bind     complement(222..246)
                     /label=R-D10
     misc_feature    226..235
                     /label=D8
     primer_bind     complement(232..256)
                     /label=R-D11
     misc_feature    236
                     /label=+1
     misc_feature    237..246
                     /label=D9
     primer_bind     complement(242..266)
                     /label=R-D12
     misc_feature    247..256
                     /label=D10
     misc_feature    257..266
                     /label=D11
     primer_bind     complement(260..287)
                     /label=2B-1R
     primer_bind     complement(261..276)
                     /label=P-Calm1-R
     misc_feature    267..276
                     /label=D12
     primer_bind     271..296
                     /label=2B-2F
     primer_bind     complement(277..298)
                     /label=Primer 1
     misc_feature    277..282
                     /label=BsiWI
     primer_bind     283..302
                     /label=Primer 5
     CDS             288..1318
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     misc_feature    548
                     /label=silent mut
     primer_bind     complement(616..650)
                     /label=R-Cre-R119STOP
     primer_bind     complement(632..651)
                     /label=R-CRE-L115-BamHI
     primer_bind     636..670
                     /label=F-Cre-R119STOP
     primer_bind     1093..1116
                     /label=F-Seq-Cre-3F1
     misc_feature    1093..1098
                     /label=ClaI
     misc_feature    1224
                     /label=mut
     primer_bind     1262..1279
                     /label=cre-SF
     primer_bind     complement(1296..1318)
                     /label=R-T5-Cre
     intron          1320..1360
                     /label=Splicing donor
                     /note="Splicing donor"
     primer_bind     complement(1338..1367)
                     /label=2B-2R
     primer_bind     complement(1341..1385)
                     /label=Primer 7
     primer_bind     complement(1341..1385)
                     /label=Primer 6
     primer_bind     1350..1387
                     /label=2B-3F
     primer_bind     complement(1356..1380)
                     /label=Primer 3
     primer_bind     1367..1401
                     /label=Primer 8
     primer_bind     1367..1401
                     /label=Primer 2
     primer_bind     1367..1401
                     /label=F-T5-JT+2272
     protein_bind    1367..1400
                     /label=loxJTZ17?2272?
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    1380..1387
                     /label=2272
     intron          1401..1492
                     /label=Splicing acceptor
                     /note="Splicing acceptor"
     primer_bind     1401..1420
                     /label=Primer 4
     primer_bind     1459..1478
                     /label=T0060_PIRES-F
     primer_bind     complement(1468..1492)
                     /label=#57-R-SA-3R1
     primer_bind     1477..1507
                     /label=A16F-NheI-F
     primer_bind     1477..1492
                     /label=Primer 10
     misc_feature    1492
                     /label=NheI?
     CDS             1493..3341
                     /codon_start=1
                     /gene="KDM5C"
                     /gene_synonym="DXS1272E; JARID1C; MRX13; MRXJ; MRXSCJ;
                     MRXSJ; SMCX; XE169"
                     /product="lysine-specific demethylase 5C isoform 1"
                     /EC_number="1.14.11.67"
                     /label=KDM5C
                     /note="isoform 1 is encoded by transcript variant 1;
                     Jumonji, AT rich interactive domain 1C (RBP2-like); 
                     lysine-specific demethylase 5C; Smcx homolog, X chromosome;
                     lysine (K)-specific demethylase 5C; JmjC domain-containing 
                     protein SMCX; Jumonji/ARID domain-containing protein 1C; 
                     histone demethylase JARID1C; Smcy homolog, X-linked; 
                     [histone H3]-trimethyl-L-lysine(4) demethylase 5C"
                     /db_xref="GI:109255243"
                     /db_xref="CCDS:CCDS14351.1"
                     /db_xref="GeneID:8242"
                     /db_xref="HGNC:HGNC:11114"
                     /db_xref="MIM:314690"
                     /protein_id="NP_004178.2"
                     /translation="GTLAVMRGLLVAGASVAPSPAVDKAQAELQELLTIAERWEEKAHL
                     CLEARQKHPPATLEAIIREAENIPVHLPNIQALKEALAKARAWIADVDEIQNGDHYPCL
                     DDLEGLVAVGRDLPVGLEELRQLELQVLTAHSWREKASKTFLKKNSCYTLLEVLCPCAD
                     AGSDSTKRSRWMEKELGLYKSDTELLGLSAQDLRDPGSVIVAFKEGEQKEKEGILQLRR
                     TNSAKPSPLASSSTASSTTSICVCGQVLAGAGALQCDLCQDWFHGRCVSVPRLLSSPRP
                     NPTSSPLLAWWEWDTKFLCPLCMRSRRPRLETILALLVALQRLPVRLPEGEALQCLTER
                     AISWQGRARQALASEDVTALLGRLAELRQRLQAEPRPEEPPNYPAAPASDPLREGSGKD
                     MPKVQGLLENGDSVTSPEKVAPEEGSGKRDLELLSSLLPQLTGPVLELPEATRAPLEEL
                     MMEGDLLEVTLDENHSIWQLLQAGQPPDLERIRTLLELEKAERHGSRARGRALERRRRR
                     KVDRGGEGDDPAREELEPKRVRSSGPEAEEVQEEEELEEETGGEGPPAPIPTTGSPSTQ
                     ENQNGLEPAEGTTSGPSAPFSTLTPRLHLPCPQQPPQQQL"
     misc_feature    1493..1494
                     /label=Feature 45
     primer_bind     1552..1568
                     /label=16B-KDM5C-SF
     primer_bind     2020..2038
                     /label=A16F-BsrGI-1F
     primer_bind     2101..2117
                     /label=A16F-SF
     primer_bind     complement(3325..3362)
                     /label=A16F-BsrGI-R
     primer_bind     complement(3328..3365)
                     /label=A16F-BsrGI-1R
     primer_bind     complement(3328..3347)
                     /label=Primer 1
     misc_feature    3342..3347
                     /label=BsrGI
     CDS             3348..3413
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(3353..3453)
                     /label=296
     terminator      3429..3860
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     primer_bind     3429..3454
                     /label=#56-F-W3SL-5F1
     primer_bind     complement(3508..3531)
                     /label=R-Seq-W3SL-5R1
     primer_bind     complement(3860..3879)
                     /label=W3SL-ampR
     misc_feature    3903..4043
                     /label=R-ITR
     rep_origin      4046..4501
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4193..4202)
                     /label=F-Seq-Cre-3F1
     promoter        4783..4887
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4888..5748
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="HSNMYPLMRQ*P**MLQ*Y*KRKSMSIQHFRVALIPFFAAFCLPV
                     FAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLS
                     RIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTT
                     IGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLT
                     LASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIV
                     VIYTTG"
     rep_origin      5919..6507
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcgctc tgcgcgaggc ggctccgccg
      181 cggcggaggg atacggcgca ccatatatat atcgcggggc gcagactcgc gctccggcag
      241 tggtgctggg agtgtcgtgg acgccgtgcc gttactcgta cgccaccatg tccaatttac
      301 tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca
      361 agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc
      421 ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc
      481 ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag
      541 taaaaaccat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc
      601 cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg
      661 ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg
      721 ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg
      781 ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata
      841 tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta
      901 gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga
      961 tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa
     1021 atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt
     1081 ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg
     1141 cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt
     1201 caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgta atgaactata
     1261 tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgattagg
     1321 taagtatcaa ggttacaaga caggtttaag gagaccaata actagtataa cttcgtatag
     1381 gatactttat agcaatttat gaaactgggc ttgtcgagac agagaagact cttgcgtttc
     1441 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac aggggcacct
     1501 tggctgtcat gcgaggactg ttggtcgcgg gtgccagtgt agcccctagc cctgctgtgg
     1561 ataaagccca ggccgagctg caggaactgc tgaccattgc tgaacgctgg gaggagaaag
     1621 cccacctctg cctggaggcc aggcagaagc atccaccagc cacacttgag gccataatcc
     1681 gtgaagcgga aaacatccct gttcacctgc ccaacatcca ggctctcaag gaggctcttg
     1741 ctaaggcccg ggcctggatt gctgatgttg atgagatcca aaatggtgac cactacccct
     1801 gcctggatga cttggagggc ctagtagctg tgggccggga cctacctgtg gggctggagg
     1861 agctgagaca gctagagcta caggtactga cagcgcactc ctggagggag aaggcctcca
     1921 agaccttcct caagaaaaat tcttgctaca cgctgctgga ggttctctgc ccatgtgcag
     1981 atgccggctc agacagcacc aagcgcagcc ggtggatgga gaaggagctg gggttgtaca
     2041 aatctgacac agagctgctg gggctgtctg cgcaggacct cagggaccca ggctctgtga
     2101 tcgtggcctt caaggagggg gaacagaagg agaaggaggg tatcctgcag ctgcgtcgca
     2161 ccaattcggc caagcccagt ccactggcat catcgagcac ggcctcctct acaacctcta
     2221 tctgtgtgtg tgggcaggtg ctggctgggg cgggagctct gcagtgtgac ctgtgtcagg
     2281 actggttcca tgggcggtgt gtgtcagtgc ctcgcctcct cagctctccg aggcccaatc
     2341 ccacctcatc cccactgctg gcctggtggg aatgggacac caaattcctg tgtccactgt
     2401 gtatgcgctc aaggcgcccg cgcctggaga ccatcctggc actgctggta gccctgcaga
     2461 gactgcctgt gcggctgccc gagggcgagg ccctgcagtg cctcacagag agggccatca
     2521 gctggcaagg ccgcgccagg caggctctgg cctctgaaga tgtgactgct cttttgggac
     2581 ggctggctga gctccgccaa cggctacagg ctgaacctag acctgaggag cctcctaact
     2641 accctgcagc ccctgcttct gaccccctca gagagggcag tggcaaggat atgcctaagg
     2701 tccagggctt actggagaat ggagacagtg tgaccagtcc tgagaaggta gccccggagg
     2761 agggctcagg taagagagat ctggagctgc tgtcctcgct gttgccacag ttgactggcc
     2821 ctgtgttgga actgcctgag gcaacccggg cccccttgga ggagctcatg atggaggggg
     2881 acctgctcga ggtgaccctg gatgagaacc acagcatatg gcagctgctg caggctggac
     2941 agcccccaga cctggagagg atccgcacac ttctggagct ggagaaggca gagcgtcacg
     3001 ggagtcgggc tcggggccgg gccctggaga ggcggcggcg gcggaaggtg gatcggggtg
     3061 gggagggcga tgacccagcc cgagaggagc tagagccaaa gagggtacgg agctcagggc
     3121 cagaggctga ggaggtccag gaggaggaag agctggagga ggagactggg ggtgagggcc
     3181 cccctgcacc catccccacc actggcagcc ccagcaccca ggagaaccag aatggcttgg
     3241 aaccggcgga agggaccact tcaggcccct cggccccttt ctccactctg actccccggc
     3301 tgcatctgcc ctgcccacag cagccgcctc agcaacagtt gtgtacagac tacaaagacc
     3361 atgacggtga ttataaagat catgacatcg actacaagga tgacgatgac aagtaagaat
     3421 tctgatgact cgagataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     3481 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     3541 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttagttct
     3601 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     3661 gggcactgac aattccgtgg tgtttatttg tgaaatttgt gatgctattg ctttatttgt
     3721 aaccatctag ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     3781 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     3841 agatgtggga ggttttttaa agatctggag accggtgtgg cacgtgcgga ccgagcggcc
     3901 gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga
     3961 ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga
     4021 gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg
     4081 cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta
     4141 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccttagcg
     4201 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     4261 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     4321 aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     4381 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     4441 acactcaact ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggtc
     4501 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     4561 acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     4621 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     4681 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     4741 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     4801 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     4861 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     4921 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     4981 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     5041 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     5101 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     5161 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     5221 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     5281 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     5341 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     5401 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     5461 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     5521 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     5581 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     5641 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     5701 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     5761 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     5821 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     5881 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     5941 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6001 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6061 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6121 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     6181 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6241 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6301 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     6361 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     6421 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     6481 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     6541 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     6601 ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
