LOCUS       Exported                7247 bp ds-DNA     circular SYN 16-MAR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7247)
  AUTHORS   SnapGene License
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Mar 20, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7247
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          748..4387
                     /organism="Homo sapiens"
                     /chromosome="19"
                     /map="19q13.33"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     promoter        157..699
                     /label=Ef1a
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
     promoter        186..397
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             410..678
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      706..711
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     misc_feature    712..714
                     /label=NcoI?
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X1"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 8 mRNAs, 11 ESTs, 75 long SRA 
                     reads, 17 Proteins, and 81% coverage of the annotated 
                     genomic feature by RNAseq alignments, including 10 samples 
                     with support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X6"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 4 mRNAs, 10 ESTs, 47 long SRA 
                     reads, 11 Proteins, and 77% coverage of the annotated 
                     genomic feature by RNAseq alignments, including 5 samples 
                     with support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X2"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 5 mRNAs, 11 ESTs, 76 long SRA 
                     reads, 13 Proteins, and 81% coverage of the annotated 
                     genomic feature by RNAseq alignments, including 28 samples 
                     with support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X9"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 6 mRNAs, 10 ESTs, 50 long SRA 
                     reads, 12 Proteins, and 77% coverage of the annotated 
                     genomic feature by RNAseq alignments, including 25 samples 
                     with support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X3"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 4 mRNAs, 4 ESTs, 48 long SRA reads,
                     11 Proteins, and 80% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 8 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X4"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 5 mRNAs, 2 ESTs, 42 long SRA reads,
                     12 Proteins, and 77% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 9 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X8"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 6 mRNAs, 1 EST, 26 long SRA reads, 
                     11 Proteins, and 72% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 10 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X10"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 4 mRNAs, 1 EST, 25 long SRA reads, 
                     2 Proteins, and 71% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 12 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X5"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 4 mRNAs, 2 ESTs, 42 long SRA reads,
                     12 Proteins, and 77% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 8 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X11"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 5 mRNAs, 1 EST, 23 long SRA reads, 
                     2 Proteins, and 70% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 7 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3"
                     /label=SH3 and multiple ankyrin repeat domains 3 mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /db_xref="GeneID:58234"
     CDS             715..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 
                     3"
                     /label=SH3 and multiple ankyrin repeat domains protein 3
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /db_xref="GI:1690553899"
                     /db_xref="CCDS:CCDS27754.1"
                     /db_xref="GeneID:58234"
                     /protein_id="NP_067398.3"
                     /translation="YPYDVPDYAG"
     CDS             715..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X2"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384763"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_030104535.1"
                     /translation="YPYDVPDYAG"
     CDS             715..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X6"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384771"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_006521287.3"
                     /translation="YPYDVPDYAG"
     CDS             715..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X4"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384767"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_006521285.3"
                     /translation="YPYDVPDYAG"
     CDS             715..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X8"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384775"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_017172203.2"
                     /translation="YPYDVPDYAG"
     CDS             715..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X9"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384777"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_030104537.1"
                     /translation="YPYDVPDYAG"
     CDS             715..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X10"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384779"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_017172205.2"
                     /translation="YPYDVPDYAG"
     CDS             715..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X5"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384769"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_006521286.3"
                     /translation="YPYDVPDYAG"
     CDS             715..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X3"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384765"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_006521284.3"
                     /translation="YPYDVPDYAG"
     CDS             715..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X11"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384781"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_017172206.2"
                     /translation="YPYDVPDYAG"
     misc_feature    715..746
                     /label=HA
     primer_bind     731..765
                     /label=A44E1-Afl2-F
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X1"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 8 mRNAs, 11 ESTs, 75 long SRA 
                     reads, 17 Proteins, and 81% coverage of the annotated 
                     genomic feature by RNAseq alignments, including 10 samples 
                     with support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X6"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 4 mRNAs, 10 ESTs, 47 long SRA 
                     reads, 11 Proteins, and 77% coverage of the annotated 
                     genomic feature by RNAseq alignments, including 5 samples 
                     with support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X2"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 5 mRNAs, 11 ESTs, 76 long SRA 
                     reads, 13 Proteins, and 81% coverage of the annotated 
                     genomic feature by RNAseq alignments, including 28 samples 
                     with support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X9"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 6 mRNAs, 10 ESTs, 50 long SRA 
                     reads, 12 Proteins, and 77% coverage of the annotated 
                     genomic feature by RNAseq alignments, including 25 samples 
                     with support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X3"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 4 mRNAs, 4 ESTs, 48 long SRA reads,
                     11 Proteins, and 80% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 8 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X4"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 5 mRNAs, 2 ESTs, 42 long SRA reads,
                     12 Proteins, and 77% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 9 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X8"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 6 mRNAs, 1 EST, 26 long SRA reads, 
                     11 Proteins, and 72% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 10 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X10"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 4 mRNAs, 1 EST, 25 long SRA reads, 
                     2 Proteins, and 71% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 12 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X5"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 4 mRNAs, 2 ESTs, 42 long SRA reads,
                     12 Proteins, and 77% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 8 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X11"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 5 mRNAs, 1 EST, 23 long SRA reads, 
                     2 Proteins, and 70% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 7 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3"
                     /label=SH3 and multiple ankyrin repeat domains 3 mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /db_xref="GeneID:58234"
     CDS             745..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 
                     3"
                     /label=SH3 and multiple ankyrin repeat domains protein 3
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /db_xref="GI:1690553899"
                     /db_xref="CCDS:CCDS27754.1"
                     /db_xref="GeneID:58234"
                     /protein_id="NP_067398.3"
                     /translation=""
     CDS             745..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X2"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384763"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_030104535.1"
                     /translation=""
     CDS             745..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X6"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384771"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_006521287.3"
                     /translation=""
     CDS             745..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X4"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384767"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_006521285.3"
                     /translation=""
     CDS             745..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X8"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384775"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_017172203.2"
                     /translation=""
     CDS             745..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X9"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384777"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_030104537.1"
                     /translation=""
     CDS             745..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X10"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384779"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_017172205.2"
                     /translation=""
     CDS             745..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X5"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384769"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_006521286.3"
                     /translation=""
     CDS             745..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X3"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384765"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_006521284.3"
                     /translation=""
     CDS             745..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X11"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384781"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_017172206.2"
                     /translation=""
     misc_feature    746..747
                     /label=Afl II
     CDS             748..4387
                     /codon_start=1
                     /gene="PRR12"
                     /gene_synonym="KIAA1205; NOC"
                     /product="proline-rich protein 12"
                     /label=PRR12
                     /db_xref="GI:153792074"
                     /db_xref="CCDS:CCDS46143.1"
                     /db_xref="GeneID:57479"
                     /db_xref="HGNC:HGNC:29217"
                     /db_xref="MIM:616633"
                     /protein_id="NP_065770.1"
                     /translation="DRNYPSAGFGDPLGAGAGWSYERSAKASLVYGSSRTSHPETDILH
                     RQAYAAPHPLQSYATNHHPAGLSGLFDTGLHHAGSAGPDASVMNLISALESRGPQPGPS
                     ASSLLSQFRSPSWQTAMHTPGPTELFISGALPGSSTFPSSSALSAYQHPASFGSRPFPV
                     PSSLSLQDPPFSPPANGLLSPHDVLHLKPSQAPTVPSSLGFERLAGGGVLGPAGLGPAQ
                     TPPYRPGPPDPPPPPRHLPTQFNLLASSSAAAAAAEQSSPQLYNFSGAAPGPPPPERAL
                     PRQDTVIKHYQRPASAQPPPPPPPAHALQHYLSCGGSYPSMGHRANLACSPLGGGEPSP
                     GAGEPSKAGPSGATAGASGRATGPEAAGGGGAGGGGGGYRPIIQSPGYKTGKGGYGAAA
                     GGATRPPPPRSTATPKCQSLGGPAAAYATGKASGAGGAGGQAYSPGQPQGLLGPQAYGQ
                     GFGGGQAQDLSKAPSYSGGPPQPPSGPPPPGLATCQSYSPDQLQGQLYGVQGEPYPGPA
                     AHSQGLPTASPSLSYSTGHSPALSGHGGGWGPSSLGGGGEASPSHIIRPLQSPPATGRP
                     PGVGSPGAPGKYLSSVLASAPFLAPPGAGSYAAGAGGYKGKGDGSELLAGPGGPPAERT
                     EDEEFLIQHLLQAPSPPRTSGADGLVGEDGAADASKGLGGSGGAGGPPGTPYELAKEDP
                     QRYHLQSVIRTSASLDEGATAALELGLGRLKEKKKGPERGGETPEGLATSVVHYGAGAK
                     ELGAFLQKSPPPPPPTAQSTQPTPHGLLLEAGGPDLPLVLPPPPPQLLPSVLSHAPSPS
                     PSASKVGVHLLEPATRDGAPQPPPPPPPPPPPMPLQLEAHLRSHGLEPAAPSPRLRPEE
                     SLDPPGAMQELLGALEPLPPAPGDTGVGPPNSEGKDPAGAYRSPSPQGTKAPRFVPLTS
                     ICFPDSLLQDEERSFFPTMEEMFGGGAADDYGKAGPPEDEGDPKAGAGPPPGPPAYDPY
                     GPYCPGRASGAGPETPGLGLDPNKPPELPSTVNAEPLGLIQSGPHQAAPPPPPPPPPPP
                     APASEPKGGLTSPIFCSTKPKKLLKTSSFHLLRRRDPPFQTPKKLYAQEYEFEADEDKA
                     DVPADIRLNPRRLPDLVSSCRSRPALSPLGDIDFCPPNPGPDGPRRRGRKPTKAKRDGP
                     PRPRGRPRIRPLEVPTTAGPASASTPTDGAKKPRGRGRGRGRKAEEA"
     primer_bind     1530..1546
                     /label=A44E-SF1
     primer_bind     complement(2799..2816)
                     /label=A44E1-1R
     primer_bind     2800..2816
                     /label=A44E2-2F
     primer_bind     complement(2914..2930)
                     /label=A44E-SR1
     primer_bind     complement(4370..4405)
                     /label=A44E2-SpeI-R
     misc_feature    4385..4387
                     /label=Feature 48
     intron          4388..4429
                     /label=Splicing donor
                     /note="Splicing donor"
     primer_bind     complement(4388..4405)
                     /label=Primer 6
     misc_feature    4388..4389
                     /label=SpeI?
     primer_bind     complement(4409..4433)
                     /label=R-SD-AgeI
     misc_feature    4434..4466
                     /label=lox JT15
     primer_bind     4440..4481
                     /label=Primer 3
     misc_feature    4446..4453
                     /label=2272
     primer_bind     complement(4484..4498)
                     /label=333A-AgeI-R
     repeat_region   4511..4651
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4726..5181
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     5316..5338
                     /label=T0056_PGEX-3
     primer_bind     5316..5338
                     /label=PGEX-3
     primer_bind     complement(5428..5447)
                     /label=_pBR322.F
     primer_bind     complement(5428..5447)
                     /label=pBR322.F
     primer_bind     complement(5444..5463)
                     /label=_PTRG-R
     primer_bind     complement(5444..5463)
                     /label=PTRG-R
     promoter        5463..5567
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5568..6428
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="*VFNISVSPLFPFLRHFAFLFLLTQKRW*K*KMLKISWVHEWVTS
                     NWISTAVRSLRVFAPKNVFQ**ALLKFCYVARYYPVLTPGKSNSVAAYTILRMTWLSTH
                     QSQKSILRMA*Q*ENYAVLP*P*VITLRPTYF*QRSEDRRS*PLFCTTWGIM*LALIVG
                     NRS*MKPYQTTSVTPRCL*QWQQRCANY*LANYLL*LPGNN**TGWRRIKLQDHFCARP
                     FRLAGLLLINLEPVSVGLAVSLQHWGQMVSPPVS*LSTRRGVRQLWMNEIDRSLR*VPH
                     *LSIGN"
     primer_bind     complement(5584..5601)
                     /label=T0089_Amp-F
     primer_bind     complement(5584..5601)
                     /label=Amp-F
     primer_bind     complement(5785..5804)
                     /label=T0010_Amp-R
     primer_bind     complement(5785..5804)
                     /label=Amp-R
     primer_bind     6320..6343
                     /label=_PrimerBlaM1
     primer_bind     6320..6343
                     /label=PrimerBlaM1
     primer_bind     complement(6525..6548)
                     /label=_PrimerBlaRV
     primer_bind     complement(6525..6548)
                     /label=PrimerBlaRV
     rep_origin      6599..7187
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtggatc tgcgatcgct ccggtgcccg
      181 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
      241 ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
      301 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
      361 cgttcttttt cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat
      421 ctctccttca cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt
      481 tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa
      541 gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg
      601 gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt
      661 tctgcgccgt tacagatcca agctgtgacc ggcgcctacc gtacggccac catgtaccct
      721 tatgacgtac ctgactatgc tggatctgac aggaactacc ccagcgccgg cttcggggac
      781 ccgctcggcg ccggggcggg atggagttac gagaggtcag cgaaagctag cttggtttat
      841 ggcagctcca ggacctcgca ccccgagacg gacatcttac accgccaggc ctatgcggcc
      901 ccccacccac tgcaaagcta tgccaccaac caccacccgg caggcctctc tggactcttc
      961 gacactggcc tccaccacgc gggctcagca gggcccgacg cctccgtcat gaacctcatc
     1021 tcggccctgg aatcccgggg cccccagcct ggcccctccg cctcctctct cctctcccag
     1081 ttccgcagtc cttcctggca aacagccatg cacacgccag gccccacgga gctcttcatc
     1141 tcgggtgccc tgccgggttc cagcaccttt ccgtcctcat ctgccctgtc ggcttaccaa
     1201 cacccggctt ccttcggcag ccgccccttc ccagtgccct cgtccctcag cctccaggac
     1261 cccccattca gccctccagc taacgggctc ctgtcccctc atgacgtgct gcacctgaag
     1321 ccctcgcagg cacccacggt gccctcttca ctgggcttcg agcgcctggc agggggcggt
     1381 gtcttggggc cagctggtct cggtccagcc cagacccccc cttaccgccc tggcccccca
     1441 gacccaccac cacctcctcg ccacctccca actcagttca acctgctggc ttcctcttcc
     1501 gctgccgccg ccgctgccga gcagtcctcc ccacagctct ataacttctc gggtgctgcc
     1561 ccgggcccac cgccacctga gcgggccctg ccacgccagg acacggtcat caagcactac
     1621 cagcggccag ccagtgccca gcccccacca cccccgccac cagcccatgc gctccagcac
     1681 tatctgagct gtggaggcag ctacccctcc atgggccacc gggccaacct ggcctgcagc
     1741 cccctgggtg gtggggagcc ctccccgggt gctggggagc ctagcaaggc tggtcccagc
     1801 ggagccacgg ctggggcatc tggccgggcc acgggccctg aggcagcagg gggcggtggg
     1861 gctgggggtg gtggtggagg ttaccgcccc atcattcagt cgcctgggta caagacgggc
     1921 aaaggtggtt atggagcagc tgccgggggt gccaccaggc cccccccacc ccgttcgacc
     1981 gccaccccca aatgtcagag cctgggtggg ccagcagccg cctatgccac tgggaaggcc
     2041 tctggggctg gaggggcagg gggccaggct tattcccccg gtcagcctca agggcttctg
     2101 ggaccccagg cctacgggca agggtttgga ggggggcagg cacaggactt gagcaaagcc
     2161 cccagctact cagggggccc cccacagccc cccagcggcc cccctcctcc tggcctggcc
     2221 acatgtcaga gctactcccc ggaccagctg caggggcagc tgtatggggt gcagggcgag
     2281 ccatacccag ggccagccgc ccactcccag gggctgccca cagccagccc ctcgctcagc
     2341 tacagtaccg gccattcccc agcgctctcg ggccatgggg gtggctgggg acccagctcc
     2401 ctgggaggcg gcggtgaggc cagcccatct cacatcattc gtccgctcca gtcaccgcct
     2461 gccaccggcc gtccacctgg agtcggctct ccaggagccc ctggcaaata cctgagctca
     2521 gtcttggcct cagcgccttt cctggcacct ccgggagctg gcagctatgc agctggagca
     2581 ggtggctaca agggcaaggg ggatggctcg gagctgctgg cgggcccagg tgggcctcct
     2641 gcggagcgca cagaggatga ggagttcctt atccagcacc tcttgcaggc gcccagccct
     2701 cctcggacct caggggcgga cggcttggtg ggcgaggacg gggcagcaga tgcctctaag
     2761 ggacttgggg ggagtggcgg ggccggggga ccaccgggta caccctacga gttggccaag
     2821 gaagaccccc agaggtacca cctgcagagt gtcatccgca ccagtgccag cctggatgag
     2881 ggtgccactg cggcactgga gctgggcctg gggaggctga aggagaagaa gaaagggcca
     2941 gagcggggtg gcgagacccc cgaggggctg gccacctctg ttgtccacta cggggcaggc
     3001 gccaaggagc tgggggcctt cttgcaaaag agccctccgc ccccacctcc cacggcccag
     3061 tctacccagc ccactcccca tggcctcctt ctggaggccg ggggccctga cctcccactg
     3121 gtgctgcctc cgcctccccc ccagctgctc ccctcggtcc tcagccatgc ccccagtccc
     3181 tctcccagcg cctccaaagt cggcgtccac ctccttgagc cagccacccg cgatggggca
     3241 ccccagccac ctccaccgcc acccccgcct ccaccaccca tgcccctgca gctcgaggcc
     3301 cacctccgca gccatggcct ggagcccgcg gcccccagcc cccgcctgcg acccgaggag
     3361 agcctggatc cgccaggcgc catgcaggaa ttgctcgggg ctctggagcc gctgcccccg
     3421 gcgcctgggg atactggcgt aggcccgcca aactcggagg gcaaggatcc cgcaggcgcc
     3481 taccgcagcc ccagcccgca aggcaccaag gcgccgcgtt tcgtgccgct cacctccatc
     3541 tgcttccctg actccttgct ccaagacgag gagcgcagct tcttccccac catggaggag
     3601 atgttcggtg gaggggccgc ggacgactac ggcaaggccg ggccacctga ggacgagggg
     3661 gaccccaagg ctggcgctgg gccacccccc ggcccccctg cttatgatcc ctatgggccc
     3721 tactgtcctg gccgggcgtc gggagccggg cccgagacac cgggcctggg cctggacccc
     3781 aacaaaccgc ctgaactgcc ctccacggtc aacgccgagc cgctgggcct gatccagagt
     3841 ggcccccacc aggcggcgcc accacccccg cctccgccac cgccgcctcc cgcgccggcc
     3901 tccgaaccca agggtggcct cacctcgccc atcttctgct ctaccaagcc aaagaagctg
     3961 ctcaagacat cctccttcca cctgctgcgg cgccgcgacc cacccttcca gacccccaag
     4021 aagctgtacg cccaggagta cgagttcgag gcggacgagg acaaggccga tgttcccgcc
     4081 gacatccgcc tcaacccccg gcgcttgcct gacctggtct ccagctgccg ctcccgtccg
     4141 gccctctcgc cactggggga catcgacttc tgcccaccca acccaggacc cgatggcccc
     4201 cggcgccgtg gccgcaagcc cacgaaggcg aaacgtgatg ggccaccccg gccacggggg
     4261 aggccccgga tccgccccct ggaggtcccg accactgcgg ggcccgcctc ggcctccacg
     4321 cccaccgatg gcgccaagaa accccggggc cggggccgag gccggggtcg aaaggctgag
     4381 gaggcaggta agtatcaagg ttacaagaca ggtttaagga gaccaataag ctattattcg
     4441 tataggatac tttatacgaa gttattaagt tatggagacc ggtgtggcac gtgcggaccg
     4501 agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc
     4561 tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag
     4621 tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc
     4681 atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg
     4741 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     4801 cttagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     4861 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     4921 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
     4981 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     5041 tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga ttttgccgat
     5101 ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     5161 aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata
     5221 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     5281 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     5341 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     5401 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     5461 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     5521 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     5581 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     5641 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     5701 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     5761 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     5821 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     5881 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     5941 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     6001 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     6061 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     6121 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     6181 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     6241 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     6301 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     6361 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     6421 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     6481 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     6541 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6601 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6661 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6721 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     6781 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6841 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6901 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6961 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     7021 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     7081 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     7141 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     7201 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//
