LOCUS       Exported                7066 bp ds-DNA     circular SYN 16-MAR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7066)
  AUTHORS   SnapGene License
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Mar 20, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7066
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          748..4206
                     /organism="Homo sapiens"
                     /chromosome="21"
                     /map="21q22.11"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     promoter        157..699
                     /label=Ef1a
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
     promoter        186..397
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             410..678
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      706..711
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     misc_feature    712..714
                     /label=NcoI?
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X1"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 8 mRNAs, 11 ESTs, 75 long SRA 
                     reads, 17 Proteins, and 81% coverage of the annotated 
                     genomic feature by RNAseq alignments, including 10 samples 
                     with support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X6"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 4 mRNAs, 10 ESTs, 47 long SRA 
                     reads, 11 Proteins, and 77% coverage of the annotated 
                     genomic feature by RNAseq alignments, including 5 samples 
                     with support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X2"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 5 mRNAs, 11 ESTs, 76 long SRA 
                     reads, 13 Proteins, and 81% coverage of the annotated 
                     genomic feature by RNAseq alignments, including 28 samples 
                     with support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X9"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 6 mRNAs, 10 ESTs, 50 long SRA 
                     reads, 12 Proteins, and 77% coverage of the annotated 
                     genomic feature by RNAseq alignments, including 25 samples 
                     with support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X3"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 4 mRNAs, 4 ESTs, 48 long SRA reads,
                     11 Proteins, and 80% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 8 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X4"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 5 mRNAs, 2 ESTs, 42 long SRA reads,
                     12 Proteins, and 77% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 9 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X8"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 6 mRNAs, 1 EST, 26 long SRA reads, 
                     11 Proteins, and 72% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 10 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X10"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 4 mRNAs, 1 EST, 25 long SRA reads, 
                     2 Proteins, and 71% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 12 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X5"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 4 mRNAs, 2 ESTs, 42 long SRA reads,
                     12 Proteins, and 77% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 8 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X11"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 5 mRNAs, 1 EST, 23 long SRA reads, 
                     2 Proteins, and 70% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 7 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            715..746
                     /product="SH3 and multiple ankyrin repeat domains 3"
                     /label=SH3 and multiple ankyrin repeat domains 3 mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /db_xref="GeneID:58234"
     CDS             715..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 
                     3"
                     /label=SH3 and multiple ankyrin repeat domains protein 3
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /db_xref="GI:1690553899"
                     /db_xref="CCDS:CCDS27754.1"
                     /db_xref="GeneID:58234"
                     /protein_id="NP_067398.3"
                     /translation="YPYDVPDYAG"
     CDS             715..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X2"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384763"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_030104535.1"
                     /translation="YPYDVPDYAG"
     CDS             715..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X6"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384771"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_006521287.3"
                     /translation="YPYDVPDYAG"
     CDS             715..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X4"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384767"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_006521285.3"
                     /translation="YPYDVPDYAG"
     CDS             715..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X8"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384775"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_017172203.2"
                     /translation="YPYDVPDYAG"
     CDS             715..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X9"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384777"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_030104537.1"
                     /translation="YPYDVPDYAG"
     CDS             715..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X10"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384779"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_017172205.2"
                     /translation="YPYDVPDYAG"
     CDS             715..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X5"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384769"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_006521286.3"
                     /translation="YPYDVPDYAG"
     CDS             715..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X3"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384765"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_006521284.3"
                     /translation="YPYDVPDYAG"
     CDS             715..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X11"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384781"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_017172206.2"
                     /translation="YPYDVPDYAG"
     misc_feature    715..746
                     /label=HA
     primer_bind     731..767
                     /label=B48E1-Afl2-F
     primer_bind     731..747
                     /label=Primer 2
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X1"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 8 mRNAs, 11 ESTs, 75 long SRA 
                     reads, 17 Proteins, and 81% coverage of the annotated 
                     genomic feature by RNAseq alignments, including 10 samples 
                     with support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X6"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 4 mRNAs, 10 ESTs, 47 long SRA 
                     reads, 11 Proteins, and 77% coverage of the annotated 
                     genomic feature by RNAseq alignments, including 5 samples 
                     with support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X2"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 5 mRNAs, 11 ESTs, 76 long SRA 
                     reads, 13 Proteins, and 81% coverage of the annotated 
                     genomic feature by RNAseq alignments, including 28 samples 
                     with support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X9"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 6 mRNAs, 10 ESTs, 50 long SRA 
                     reads, 12 Proteins, and 77% coverage of the annotated 
                     genomic feature by RNAseq alignments, including 25 samples 
                     with support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X3"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 4 mRNAs, 4 ESTs, 48 long SRA reads,
                     11 Proteins, and 80% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 8 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X4"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 5 mRNAs, 2 ESTs, 42 long SRA reads,
                     12 Proteins, and 77% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 9 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X8"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 6 mRNAs, 1 EST, 26 long SRA reads, 
                     11 Proteins, and 72% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 10 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X10"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 4 mRNAs, 1 EST, 25 long SRA reads, 
                     2 Proteins, and 71% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 12 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X5"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 4 mRNAs, 2 ESTs, 42 long SRA reads,
                     12 Proteins, and 77% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 8 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3, 
                     transcript variant X11"
                     /label=SH3 and multiple ankyrin repeat domains 3, tran
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon. Supporting evidence 
                     includes similarity to: 5 mRNAs, 1 EST, 23 long SRA reads, 
                     2 Proteins, and 70% coverage of the annotated genomic 
                     feature by RNAseq alignments, including 7 samples with 
                     support for all annotated introns"
                     /db_xref="GeneID:58234"
     mRNA            745..746
                     /product="SH3 and multiple ankyrin repeat domains 3"
                     /label=SH3 and multiple ankyrin repeat domains 3 mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /db_xref="GeneID:58234"
     CDS             745..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 
                     3"
                     /label=SH3 and multiple ankyrin repeat domains protein 3
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /db_xref="GI:1690553899"
                     /db_xref="CCDS:CCDS27754.1"
                     /db_xref="GeneID:58234"
                     /protein_id="NP_067398.3"
                     /translation=""
     CDS             745..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X2"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384763"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_030104535.1"
                     /translation=""
     CDS             745..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X6"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384771"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_006521287.3"
                     /translation=""
     CDS             745..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X4"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384767"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_006521285.3"
                     /translation=""
     CDS             745..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X8"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384775"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_017172203.2"
                     /translation=""
     CDS             745..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X9"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384777"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_030104537.1"
                     /translation=""
     CDS             745..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X10"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384779"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_017172205.2"
                     /translation=""
     CDS             745..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X5"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384769"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_006521286.3"
                     /translation=""
     CDS             745..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X3"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384765"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_006521284.3"
                     /translation=""
     CDS             745..746
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein 3
                     isoform X11"
                     /label=SH3 and multiple ankyrin repeat domains protein
                     /note="Derived by automated computational analysis using 
                     gene prediction method: Gnomon."
                     /db_xref="GI:1720384781"
                     /db_xref="GeneID:58234"
                     /protein_id="XP_017172206.2"
                     /translation=""
     misc_feature    746..747
                     /label=Afl II
     CDS             748..4206
                     /codon_start=1
                     /gene="ITSN1"
                     /gene_synonym="ITSN; SH3D1A; SH3P17"
                     /product="intersectin-1 isoform ITSN-l"
                     /label=ITSN1
                     /note="isoform ITSN-l is encoded by transcript variant 1;
                     human intersectin-SH3 domain-containing protein SH3P17; Src
                     homology 3 domain-containing protein; SH3 domain-containing
                     protein 1A; intersectin 1 (SH3 domain protein)"
                     /db_xref="GI:47717123"
                     /db_xref="CCDS:CCDS33545.1"
                     /db_xref="GeneID:6453"
                     /db_xref="HGNC:HGNC:6183"
                     /db_xref="MIM:602442"
                     /protein_id="NP_003015.2"
                     /translation="AQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARN
                     FFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQ
                     PVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPTLVSSVPTAAVPPLANGAPP
                     VIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRL
                     KYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILA
                     MHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQ
                     LEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQE
                     QERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLN
                     QRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRE
                     LRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKEL
                     ARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQE
                     RKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEE
                     KGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESR
                     SHDEITIQPGDIVMVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTD
                     STSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQP
                     SLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNK
                     NDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVAS
                     PAAKPVVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKAGVFPSNY
                     VRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWE
                     GELQARGKKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQ"
     primer_bind     1453..1469
                     /label=B48E-SF1
     primer_bind     1504..1520
                     /label=B48A-ITSN1-SF
     primer_bind     2100..2117
                     /label=B48E-SF2
     primer_bind     complement(2443..2459)
                     /label=B48A-ITSN1-SR
     primer_bind     complement(3257..3276)
                     /label=B48E1-1R
     primer_bind     3260..3279
                     /label=B48E2-2F
     primer_bind     complement(3489..3506)
                     /label=B48E-SR1
     primer_bind     complement(4188..4224)
                     /label=B48E2-SpeI-R
     exon            4199..4206
                     /gene="ITSN1"
                     /gene_synonym="ITSN; SH3D1A; SH3P17"
                     /inference="alignment:same species:2.1.0"
     misc_feature    4204..4206
                     /label=Feature 66
     intron          4207..4248
                     /label=Splicing donor
                     /note="Splicing donor"
     primer_bind     complement(4207..4224)
                     /label=Primer 6
     misc_feature    4207..4208
                     /label=SpeI?
     primer_bind     complement(4228..4252)
                     /label=R-SD-AgeI
     misc_feature    4253..4285
                     /label=lox JT15
     primer_bind     4259..4300
                     /label=Primer 3
     misc_feature    4265..4272
                     /label=2272
     primer_bind     complement(4303..4317)
                     /label=333A-AgeI-R
     repeat_region   4330..4470
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4545..5000
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     5135..5157
                     /label=T0056_PGEX-3
     primer_bind     5135..5157
                     /label=PGEX-3
     primer_bind     complement(5247..5266)
                     /label=_pBR322.F
     primer_bind     complement(5247..5266)
                     /label=pBR322.F
     primer_bind     complement(5263..5282)
                     /label=_PTRG-R
     primer_bind     complement(5263..5282)
                     /label=PTRG-R
     promoter        5282..5386
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5387..6247
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="*VFNISVSPLFPFLRHFAFLFLLTQKRW*K*KMLKISWVHEWVTS
                     NWISTAVRSLRVFAPKNVFQ**ALLKFCYVARYYPVLTPGKSNSVAAYTILRMTWLSTH
                     QSQKSILRMA*Q*ENYAVLP*P*VITLRPTYF*QRSEDRRS*PLFCTTWGIM*LALIVG
                     NRS*MKPYQTTSVTPRCL*QWQQRCANY*LANYLL*LPGNN**TGWRRIKLQDHFCARP
                     FRLAGLLLINLEPVSVGLAVSLQHWGQMVSPPVS*LSTRRGVRQLWMNEIDRSLR*VPH
                     *LSIGN"
     primer_bind     complement(5403..5420)
                     /label=T0089_Amp-F
     primer_bind     complement(5403..5420)
                     /label=Amp-F
     primer_bind     complement(5604..5623)
                     /label=T0010_Amp-R
     primer_bind     complement(5604..5623)
                     /label=Amp-R
     primer_bind     6139..6162
                     /label=_PrimerBlaM1
     primer_bind     6139..6162
                     /label=PrimerBlaM1
     primer_bind     complement(6344..6367)
                     /label=_PrimerBlaRV
     primer_bind     complement(6344..6367)
                     /label=PrimerBlaRV
     rep_origin      6418..7006
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtggatc tgcgatcgct ccggtgcccg
      181 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
      241 ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
      301 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
      361 cgttcttttt cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat
      421 ctctccttca cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt
      481 tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa
      541 gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg
      601 gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt
      661 tctgcgccgt tacagatcca agctgtgacc ggcgcctacc gtacggccac catgtaccct
      721 tatgacgtac ctgactatgc tggatctgct cagtttccaa caccttttgg tggcagcctg
      781 gatatctggg ccataactgt agaggaaaga gcgaagcatg atcagcagtt ccatagttta
      841 aagccaatat ctggattcat tactggtgat caagctagaa actttttttt tcaatctggg
      901 ttacctcaac ctgttttagc acagatatgg gcactagctg acatgaataa tgatggaaga
      961 atggatcaag tggagttttc catagctatg aaacttatca aactgaagct acaaggatat
     1021 cagctaccct ctgcacttcc ccctgtcatg aaacagcaac cagttgctat ttctagcgca
     1081 ccagcatttg gtatgggagg tatcgccagc atgccaccgc ttacagctgt tgctccagtg
     1141 ccaatgggat ccattccagt tgttggaatg tctccaaccc tagtatcttc tgttcccaca
     1201 gcagctgtgc cccccctggc taacggggct ccccctgtta tacaacctct gcctgcattt
     1261 gctcatcctg cagccacatt gccaaagagt tcttccttta gtagatctgg tccagggtca
     1321 caactaaaca ctaaattaca aaaggcacag tcatttgatg tggccagtgt cccaccagtg
     1381 gcagagtggg ctgttcctca gtcatcaaga ctgaaataca ggcaattatt caatagtcat
     1441 gacaaaacta tgagtggaca cttaacaggt ccccaagcaa gaactattct tatgcagtca
     1501 agtttaccac aggctcagct ggcttcaata tggaatcttt ctgacattga tcaagatgga
     1561 aaacttacag cagaggaatt tatcctggca atgcacctca ttgatgtagc tatgtctggc
     1621 caaccactgc cacctgtcct gcctccagaa tacattccac cttcttttag aagagttcga
     1681 tctggcagtg gtatatctgt cataagctca acatctgtag atcagaggct accagaggaa
     1741 ccagttttag aagatgaaca acaacaatta gaaaagaaat tacctgtaac gtttgaagat
     1801 aagaagcggg agaactttga acgtggcaac ctggaactgg agaaacgaag gcaagctctc
     1861 ctggaacagc agcgcaagga gcaggagcgc ctggcccagc tggagcgggc ggagcaggag
     1921 aggaaggagc gtgagcgcca ggagcaagag cgcaaaagac aactggaact ggagaagcaa
     1981 ctggaaaagc agcgggagct agaacggcag agagaggagg agaggaggaa agaaattgag
     2041 aggcgagagg ctgcaaaacg ggaacttgaa aggcaacgac aacttgagtg ggaacggaat
     2101 cgaaggcaag aactactaaa tcaaagaaac aaagaacaag aggacatagt tgtactgaaa
     2161 gcaaagaaaa agactttgga atttgaatta gaagctctaa atgataaaaa gcatcaacta
     2221 gaagggaaac ttcaagatat cagatgtcga ttgaccaccc aaaggcaaga aattgagagc
     2281 acaaacaaat ctagagagtt gagaattgcc gaaatcaccc atctacagca acaattacag
     2341 gaatctcagc aaatgcttgg aagacttatt ccagaaaaac agatactcaa tgaccaatta
     2401 aaacaagttc agcagaacag tttgcacaga gattcacttg ttacacttaa aagagcctta
     2461 gaagcaaaag aactagctcg gcagcaccta cgagaccaac tggatgaagt ggagaaagaa
     2521 actagatcaa aactacagga gattgatatt ttcaataatc agctgaagga actaagagaa
     2581 atacacaata agcaacaact ccagaagcaa aagtccatgg aggctgaacg actgaaacag
     2641 aaagaacaag aacgaaagat catagaatta gaaaaacaaa aagaagaagc ccaaagacga
     2701 gctcaggaaa gggacaagca gtggctggag catgtgcagc aggaggacga gcatcagaga
     2761 ccaagaaaac tccacgaaga ggaaaaactg aaaagggagg agagtgtcaa aaagaaggat
     2821 ggcgaggaaa aaggcaaaca ggaagcacaa gacaagctgg gtcggctttt ccatcaacac
     2881 caagaaccag ctaagccagc tgtccaggca ccctggtcca ctgcagaaaa aggtccactt
     2941 accatttctg cacaggaaaa tgtaaaagtg gtgtattacc gggcactgta cccctttgaa
     3001 tccagaagcc atgatgaaat cactatccag ccaggagaca tagtcatggt ggatgaaagc
     3061 caaactggag aacccggctg gcttggagga gaattaaaag gaaagacagg gtggttccct
     3121 gcaaactatg cagagaaaat cccagaaaat gaggttcccg ctccagtgaa accagtgact
     3181 gattcaacat ctgcccctgc ccccaaactg gccttgcgtg agacccccgc ccctttggca
     3241 gtaacctctt cagagccctc cacgacccct aataactggg ccgacttcag ctccacgtgg
     3301 cccaccagca cgaatgagaa accagaaacg gataactggg atgcatgggc agcccagccc
     3361 tctctcaccg ttccaagtgc cggccagtta aggcagaggt ccgcctttac tccagccacg
     3421 gccactggct cctccccgtc tcctgtgcta ggccagggtg aaaaggtgga ggggctacaa
     3481 gctcaagccc tatatccttg gagagccaaa aaagacaacc acttaaattt taacaaaaat
     3541 gatgtcatca ccgtcctgga acagcaagac atgtggtggt ttggagaagt tcaaggtcag
     3601 aagggttggt tccccaagtc ttacgtgaaa ctcatttcag ggcccataag gaagtctaca
     3661 agcatggatt ctggttcttc agagagtcct gctagtctaa agcgagtagc ctctccagca
     3721 gccaagccgg tcgtttcggg agaagaattt attgccatgt acacttacga gagttctgag
     3781 caaggagatt taacctttca gcaaggggat gtgattttgg ttaccaagaa agatggtgac
     3841 tggtggacag gaacagtggg cgacaaggcc ggagtcttcc cttctaacta tgtgaggctt
     3901 aaagattcag agggctctgg aactgctggg aaaacaggga gtttaggaaa aaaacctgaa
     3961 attgcccagg ttattgcctc atacaccgcc accggccccg agcagctcac tctcgcccct
     4021 ggtcagctga ttttgatccg aaaaaagaac ccaggtggat ggtgggaagg agagctgcaa
     4081 gcacgtggga aaaagcgcca gataggctgg ttcccagcta attatgtaaa gcttctaagc
     4141 cctgggacga gcaaaatcac tccaacagag ccacctaagt caacagcatt agcggcagtg
     4201 tgccaggtaa gtatcaaggt tacaagacag gtttaaggag accaataagc tattattcgt
     4261 ataggatact ttatacgaag ttattaagtt atggagaccg gtgtggcacg tgcggaccga
     4321 gcggccgcag gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct
     4381 cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt
     4441 gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca
     4501 tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc
     4561 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     4621 ttagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     4681 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     4741 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     4801 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     4861 ggaacaacac tcaactctat ctcgggctat tcttttgatt tataagggat tttgccgatt
     4921 tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     4981 atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag
     5041 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     5101 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     5161 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     5221 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     5281 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     5341 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     5401 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     5461 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     5521 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     5581 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     5641 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     5701 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     5761 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     5821 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     5881 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     5941 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     6001 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     6061 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     6121 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     6181 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     6241 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     6301 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     6361 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6421 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6481 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     6541 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     6601 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     6661 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     6721 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     6781 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     6841 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     6901 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     6961 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     7021 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgt
//
