LOCUS       pAAV-BEC_enhance        5640 bp DNA     circular SYN 17-MAR-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Artificial Intelligence, Drug Discovery lab
  TITLE     Direct Submission
  JOURNAL   Exported Mar 17, 2025 from SnapGene Viewer 7.2.0
            https://www.snapgene.com
COMMENT     Sequence Label: pAAV-BEC Enhancer-minBG-iCRE(297T)-4x6T
FEATURES             Location/Qualifiers
     source          1..5640
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   2..131
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     enhancer        146..164
                     /label=CMV enhancer (fragment)
                     /note="color: #a6acb3"
     gene            165..665
                     /label=cCREs89763
                     /note="color: #3366ff; direction: RIGHT"
     promoter        672..724
                     /label=MinBG
                     /label=MiniBG
                     /note="color: #ffffff"
     CDS             753..779
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /label=SV40 NLS
                     /note="/vntifkey=21"
                     /note="color: #993366"
                     /translation="MVPKKKRKV"
     CDS             780..1808
                     /codon_start=1
                     /label=iCre(297T)
                     /note="/vntifkey=21"
                     /note="color: #993366"
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAATDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     variation       1665..1667
                     /label=R297T
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    1728..1730
                     /label=V318
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    1796
                     /label=G-->A silent mutation to remove XhoI site
                     /label=G-->A\silent\mutation\to\remove\XhoI\site
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     misc_feature    1830..2358
                     /label=4x6T miR cassette
                     /note="color: #ff9900"
     misc_feature    1830..1849
                     /label=miR124 T
                     /note="color: #cc99ff"
     misc_feature    1850..1872
                     /label=miR137-3p T
                     /note="color: #aaffff"
     misc_feature    1873..1894
                     /label=miR369-5p T
                     /note="color: #ccffcc"
     misc_feature    1895..1916
                     /label=miR329-3p
                     /note="color: #ffcc00"
     misc_feature    1917..1938
                     /label=miR431-3p T
                     /note="color: #ff99cc"
     misc_feature    1939..1960
                     /label=miR126-3p T
                     /note="color: #99ccff"
     misc_feature    1961..1982
                     /label=miR126-3p T
                     /note="color: #ffff99"
     misc_feature    1983..2002
                     /label=miR124 T
                     /note="color: #33cccc"
     misc_feature    2003..2025
                     /label=miR137-3p T
                     /note="color: #99cc00"
     misc_feature    2026..2047
                     /label=miR369-5p T
                     /note="color: #ff00ff"
     misc_feature    2048..2069
                     /label=miR329-3p
                     /note="color: #33cccc"
     misc_feature    2070..2091
                     /label=miR431-3p T
                     /note="color: #339966"
     misc_feature    2092..2111
                     /label=miR124 T
                     /note="color: #99cc00"
     misc_feature    2112..2134
                     /label=miR137-3p T
                     /note="color: #00ffff"
     misc_feature    2135..2156
                     /label=miR369-5p T
                     /note="color: #cc99ff"
     misc_feature    2157..2178
                     /label=miR329-3p
                     /note="color: #ff99cc"
     misc_feature    2179..2200
                     /label=miR431-3p T
                     /note="color: #c0c0c0"
     misc_feature    2206..2227
                     /label=miR126-3p T
                     /note="color: #ffcc99"
     misc_feature    2228..2249
                     /label=miR126-3p T
                     /note="color: #ccffcc"
     misc_feature    2250..2269
                     /label=miR124 T
                     /note="color: #ccffff"
     misc_feature    2270..2292
                     /label=miR137-3p T
                     /note="color: #99cc00"
     misc_feature    2293..2314
                     /label=miR369-5p T
                     /note="color: #00ccff"
     misc_feature    2315..2336
                     /label=miR329-3p
                     /note="color: #cc99ff"
     misc_feature    2337..2358
                     /label=miR431-3p T
                     /note="color: #ffcc00"
     enhancer        2386..2864
                     /label=hGH-PA
                     /note="color: #a6acb3"
     repeat_region   2904..3044
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     rep_origin      3119..3574
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     primer_bind     3416..3437
                     /label=F1ori-F
                     /note="color: black; sequence: GTGGACTCTTGTTCCAAACTGG"
     gene            3736..4596
                     /gene="Ampicillin"
                     /label=Ampicillin
                     /note="color: #a6acb3; direction: RIGHT"
     promoter        3856..3960
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             join(3961..4029,4030..4821)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /note="This feature has 2 segments: 1: 3961 .. 4029 /
                     #ccffcc / signal sequence 2: 4030 .. 4821 / #ccffcc"
                     /note="This feature has 2 segments:
                        1: 3961 .. 4029 / #993366
                        2: 4030 .. 4821 / #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4992..5580
                     /label=ori
                     /note="color: #ffff00"
     primer_bind     5481..5500
                     /label=pBR322-ori-F
                     /note="pBR322 orgion primer, Forward"
                     /note="color: black; sequence: GGGAAACGCCTGGTATCTTT"
ORIGIN
        1 tcctgcaggc agctgcgcgc tcgctcgctc actgaggccg cccgggcgtc gggcgacctt
       61 tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca actccatcac
      121 taggggttcc tgcggccgca cgcgtacatt gattattgac tagtagggag cctggaattc
      181 cctggtgaaa ctgggcttta aagacaatgt aggttatcct ggtgggatgg tatacaagga
      241 taaccccagc actgggatgg gaggattacc acacattcaa ggctaagctg gtttcatagt
      301 gagattctgt ctccaaaaca cacagaggta gctcagttat tagagagctt gcccagcctg
      361 tgttaaaccc tgtgttaaag ccttggcact ccaggaggtg gaggcaggaa gatcagaagt
      421 tcaaagacat cctcagctat gcaacaaggg aaaccccacc catctcaaaa gcaaacaggc
      481 aagaaactga agttttattc ctcacattcc tgagactaga aactaaagca cacatgcctg
      541 ccctgtgaga caggcattta tctccttcaa atagatcctg tgaccctctt cccttacaca
      601 ggctttgcac ctgcccctcc ttacccacaa ggctctggga catccaccca cagtacctct
      661 accttgagct cgggctgggc ataaaagtca gggcagagcc atctattgct tacatttgct
      721 tctgggatcc agatctttcg aagctagcca ccatggtgcc caagaagaag aggaaagtct
      781 ccaacctgct gactgtgcac caaaacctgc ctgccctccc tgtggatgcc acctctgatg
      841 aagtcaggaa gaacctgatg gacatgttca gggacaggca ggccttctct gaacacacct
      901 ggaagatgct cctgtctgtg tgcagatcct gggctgcctg gtgcaagctg aacaacagga
      961 aatggttccc tgctgaacct gaggatgtga gggactacct cctgtacctg caagccagag
     1021 gcctggctgt gaagaccatc caacagcacc tgggccagct caacatgctg cacaggagat
     1081 ctggcctgcc tcgcccttct gactccaatg ctgtgtccct ggtgatgagg agaatcagaa
     1141 aggagaatgt ggatgctggg gagagagcca agcaggccct ggcctttgaa cgcactgact
     1201 ttgaccaagt cagatccctg atggagaact ctgacagatg ccaggacatc aggaacctgg
     1261 ccttcctggg cattgcctac aacaccctgc tgcgcattgc cgaaattgcc agaatcagag
     1321 tgaaggacat ctcccgcacc gatggtggga gaatgctgat ccacattggc aggaccaaga
     1381 ccctggtgtc cacagctggt gtggagaagg ccctgtccct gggggttacc aagctggtgg
     1441 agagatggat ctctgtgtct ggtgtggctg atgaccccaa caactacctg ttctgccggg
     1501 tcagaaagaa tggtgtggct gccccttctg ccacctccca actgtccacc cgggccctgg
     1561 aagggatctt tgaggccacc caccgcctga tctatggtgc caaggatgac tctgggcaga
     1621 gatacctggc ctggtctggc cactctgcca gagtgggtgc tgccaccgac atggccaggg
     1681 ctggtgtgtc catccctgaa atcatgcagg ctggtggctg gaccaatgtg aacattgtga
     1741 tgaactacat cagaaacctg gactctgaga ctggggccat ggtgaggctg ctcgaagatg
     1801 gggactgagg cgcgccgtga tgacatatgg gcattcaccg cgtgccttac tacgcgtatt
     1861 cttaagcaat aagcgaatat aacacggtcg atctaaaaag gttagctggg tgtgttagaa
     1921 gccctgcaag acgacctgcg cattattact cacggtacga cgcattatta ctcacggtac
     1981 gaggcattca ccgcgtgcct tactacgcgt attcttaagc aataagcgaa tataacacgg
     2041 tcgatctaaa aaggttagct gggtgtgtta gaagccctgc aagacgacct gggcattcac
     2101 cgcgtgcctt actacgcgta ttcttaagca ataagcgaat ataacacggt cgatctaaaa
     2161 aggttagctg ggtgtgttag aagccctgca agacgacctg ggtaccgcat tattactcac
     2221 ggtacgacgc attattactc acggtacgag gcattcaccg cgtgccttac tacgcgtatt
     2281 cttaagcaat aagcgaatat aacacggtcg atctaaaaag gttagctggg tgtgttagaa
     2341 gccctgcaag acgacctggg taccaggtaa ccgctcgaga gatctacggg tggcatccct
     2401 gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc
     2461 ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta
     2521 tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc tgtagggcct
     2581 gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc actgcaatct
     2641 ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg ggattccagg
     2701 catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt ttcaccatat
     2761 tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg cctcccaaat
     2821 tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgatt ttgtaggtaa
     2881 ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct
     2941 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
     3001 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta
     3061 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac
     3121 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     3181 acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     3241 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     3301 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
     3361 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     3421 ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa
     3481 gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     3541 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc
     3601 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     3661 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     3721 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     3781 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     3841 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     3901 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     3961 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     4021 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     4081 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     4141 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     4201 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     4261 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     4321 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     4381 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     4441 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     4501 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     4561 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     4621 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     4681 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     4741 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     4801 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     4861 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     4921 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     4981 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5041 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5101 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     5161 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     5221 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     5281 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     5341 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     5401 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     5461 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     5521 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     5581 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
//
