LOCUS       Exported               10534 bp ds-DNA     circular SYN 28-MAR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10534)
  AUTHORS   Zhao Zhang
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 28, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..10534
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        236..615
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        617..815
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             833..1013
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1057..1182
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1675..1908
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2093..2137
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2389..2506
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2529..2832
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2833..3036
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3033..3058
                     /label=pLNCX5' primer
     regulatory      3132..3141
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3141..3206
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3225..4847
                     /label=hGpr75
     intron          4934..5163
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     misc_feature    5340..5928
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(5811..5822)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6335..6515
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6547..6771
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6817..7245
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        7259..7589
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7440..7575
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7637..7684
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7703..8077
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8207..8328
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8377..8393)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8401..8417
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8425..8455)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8470..8491
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8779..9367)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9538..10398)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10399..10503)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgacggatcg ggagatctcc cgatccccta tggtcgactc tcagtacaat ctgctctgat
       61 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
      121 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
      181 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat
      241 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
      301 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
      361 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
      421 cattgacgtc aatgggtgga ctatttacgg taaactgccc acttggcagt acatcaagtg
      481 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
      541 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
      601 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
      661 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
      721 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
      781 ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct
      841 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      901 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      961 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     1021 ccgaacaggg acctgaaagc gaaagggaaa ccagagctct ctcgacgcag gactcggctt
     1081 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     1141 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     1201 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     1261 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     1321 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     1381 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     1441 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     1501 aagaccaccg cacagcaagc ggccgctgat cttcagactt ggaggaggag atatgaggga
     1561 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1621 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1681 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcct caatgacgct
     1741 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1801 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1861 agcaagaatc ctagctgtgg aaagatacct aaaggatcaa cagctcctag ggatttgggg
     1921 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1981 atctctggaa cagatctgga atcacacgac ctggatggag tgggacagag aaattaacaa
     2041 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     2101 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     2161 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     2221 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     2281 tcagacccac ctcccaatcc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     2341 tggagagaga gacagagaca gatccattcg attagtgaac ggatcaactt ttaaaagaaa
     2401 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2461 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg ataagcttgg
     2521 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     2581 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     2641 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     2701 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     2761 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     2821 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     2881 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     2941 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     3001 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     3061 tggagacgcc atccacgctg ttttgacctc catagaagac accgactgag ctccttaagg
     3121 ttaactctag agccaccatg gactacaaag accatgacgg tgattataaa gatcatgata
     3181 tcgattacaa ggatgacgat gacaagtcta gagaattcgg atccatgaac tcaacaggcc
     3241 accttcagga tgcccccaat gccacctcgc tccatgtgcc tcactcacag gaaggaaaca
     3301 gcacctctct ccaggagggt cttcaggatc tcatccacac agccaccttg gtgacctgta
     3361 cttttctact ggcggtcatc ttctgcctgg gttcctatgg caacttcatt gtcttcttgt
     3421 ccttcttcga tccagccttc aggaaattca gaaccaactt tgatttcatg atcctgaacc
     3481 tgtccttctg tgacctcttc atttgtggag tgacagcccc catgttcacc tttgtgttat
     3541 tcttcagctc agccagtagt atcccggatg ctttctgctt cactttccat ctcaccagtt
     3601 caggcttcat catcatgtct ctgaagacag tggcagtgat cgccctgcac cggctccgga
     3661 tggtgttggg gaaacagcct aatcgcacgg cctcctttcc ctgcaccgta ctcctcaccc
     3721 tgcttctctg ggccaccagt ttcacccttg ccaccttggc taccttgaaa accagcaagt
     3781 cccacctctg tcttcccatg tccagtctga ttgctggaaa agggaaagcc attttgtctc
     3841 tctatgtggt cgacttcacc ttctgtgttg ctgtggtctc tgtctcttac atcatgattg
     3901 ctcagaccct gcggaagaac gctcaagtca gaaagtgccc ccctgtaatc acagtcgatg
     3961 cttccagacc acagcctttc atgggggtcc ctgtgcaggg aggtggagat cccatccagt
     4021 gtgccatgcc ggctctgtat aggaaccaga attacaacaa actgcagcac gttcagaccc
     4081 gtggatatac caagagtccc aaccaactgg tcacccctgc agcaagccga ctccagctcg
     4141 tatcagccat caacctctcc actgccaagg attccaaagc cgtggtcacc tgtgtgatca
     4201 ttgtgctgtc agtcctggtg tgctgtcttc cactggggat ttccttggta caggtggttc
     4261 tctccagcaa tgggagcttc attctttacc agtttgaatt gtttggattt actcttatat
     4321 ttttcaagtc aggattaaac ccttttatat attctcggaa cagtgcaggg ctgagaagga
     4381 aagtgctctg gtgcctccaa tacataggcc tgggtttttt ctgctgcaaa caaaagactc
     4441 gacttcgagc catgggaaaa gggaacctcg aagtcaacag aaacaaatcc tcccatcatg
     4501 aaacaaactc tgcctacatg ttatctccaa agccacagaa gaaatttgtg gaccaggctt
     4561 gtggcccaag tcattcaaaa gaaagtatgg tgagtcccaa gatctctgct ggacatcaac
     4621 actgtggtca gagcagctcg acccccatca acactcggat tgaaccttac tacagcatct
     4681 ataacagcag cccttcccag gaggagagca gcccatgtaa cttacagcca gtaaactctt
     4741 ttggatttgc caattcatat attgccatgc attatcacac cactaatgac ttagtgcagg
     4801 aatatgacag cacttcagcc aagcagattc cagtcccctc cgtttaactc gagccatgga
     4861 agcttgatat ctaactgact gaaccggtgg taccgaggaa ttaattcgct gtctgcgagg
     4921 gccagctgtt ggggtgagta ctccctctca aaagcgggca tgacttctgc gctaagattg
     4981 tcagtttcca aaaacgagga ggatttgata ttcacctggc ccgcggtgat gcctttgagg
     5041 gtggccgcgt ccatctggtc agaaaagaca atctttttgt tgtcaagctt gaggtgtggc
     5101 aggcttgaga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
     5161 caggtgtcca ctcccaggtc caactgcagg tcgagcatgc atctagggcg gccaattccg
     5221 ccgatcctgg aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat
     5281 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcagaca
     5341 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     5401 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     5461 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     5521 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     5581 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     5641 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     5701 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     5761 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     5821 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     5881 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattcgag
     5941 ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     6001 agaaaagggg ggactggaag ggctaattca ctcccaaaga agacaagata tccttgatct
     6061 gtggatctac cacacacaag gctacttccc tgattgacag aactacacac cagggccagg
     6121 ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa
     6181 gatagaagag gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg
     6241 gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca
     6301 tcacgtggcc cgagagctgc atccggactg tactgggtct ctctggttag accagatctg
     6361 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     6421 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     6481 cagacccttt tagtcagtgt ggaaaatctc tagcagggcc gctttaaacc cgctgatcag
     6541 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     6601 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     6661 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     6721 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     6781 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     6841 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     6901 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     6961 ctctaaatcg gggcatccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     7021 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     7081 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     7141 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgggg atttcggcct
     7201 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     7261 gtgtcagtta gggtgtggaa agtccccagg ctccccaggc aggcagaagt atgcaaagca
     7321 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     7381 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     7441 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     7501 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     7561 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     7621 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
     7681 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc
     7741 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt
     7801 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt
     7861 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga
     7921 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc
     7981 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg
     8041 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc
     8101 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     8161 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     8221 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     8281 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     8341 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     8401 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     8461 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     8521 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     8581 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     8641 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     8701 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     8761 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     8821 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8881 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     8941 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc
     9001 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     9061 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     9121 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     9181 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     9241 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     9301 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     9361 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     9421 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     9481 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     9541 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     9601 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     9661 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     9721 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     9781 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     9841 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     9901 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     9961 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    10021 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    10081 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    10141 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    10201 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    10261 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    10321 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    10381 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    10441 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    10501 gcgcacattt ccccgaaaag tgccacctga cgtc
//
