LOCUS       Exported               10527 bp ds-DNA     circular SYN 28-MAR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10527)
  AUTHORS   Zhao Zhang
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 28, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..10527
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        236..615
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        617..815
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             833..1013
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1057..1182
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1675..1908
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2093..2137
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2389..2506
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2529..2832
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2833..3036
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3033..3058
                     /label=pLNCX5' primer
     regulatory      3132..3141
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3141..3206
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3225..4840
                     /label=hGpr75-p.Ala110fs
     intron          4927..5156
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     misc_feature    5333..5921
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(5804..5815)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6328..6508
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6540..6764
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6810..7238
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        7252..7582
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7433..7568
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7630..7677
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7696..8070
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8200..8321
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8370..8386)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8394..8410
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8418..8448)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8463..8484
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8772..9360)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9531..10391)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10392..10496)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgacggatcg ggagatctcc cgatccccta tggtcgactc tcagtacaat ctgctctgat
       61 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
      121 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
      181 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat
      241 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
      301 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
      361 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
      421 cattgacgtc aatgggtgga ctatttacgg taaactgccc acttggcagt acatcaagtg
      481 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
      541 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
      601 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
      661 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
      721 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
      781 ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct
      841 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      901 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      961 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     1021 ccgaacaggg acctgaaagc gaaagggaaa ccagagctct ctcgacgcag gactcggctt
     1081 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     1141 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     1201 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     1261 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     1321 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     1381 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     1441 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     1501 aagaccaccg cacagcaagc ggccgctgat cttcagactt ggaggaggag atatgaggga
     1561 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1621 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1681 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcct caatgacgct
     1741 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1801 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1861 agcaagaatc ctagctgtgg aaagatacct aaaggatcaa cagctcctag ggatttgggg
     1921 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1981 atctctggaa cagatctgga atcacacgac ctggatggag tgggacagag aaattaacaa
     2041 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     2101 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     2161 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     2221 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     2281 tcagacccac ctcccaatcc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     2341 tggagagaga gacagagaca gatccattcg attagtgaac ggatcaactt ttaaaagaaa
     2401 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2461 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg ataagcttgg
     2521 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     2581 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     2641 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     2701 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     2761 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     2821 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     2881 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     2941 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     3001 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     3061 tggagacgcc atccacgctg ttttgacctc catagaagac accgactgag ctccttaagg
     3121 ttaactctag agccaccatg gactacaaag accatgacgg tgattataaa gatcatgata
     3181 tcgattacaa ggatgacgat gacaagtcta gagaattcgg atccatgaac tcaacaggcc
     3241 accttcagga tgcccccaat gccacctcgc tccatgtgcc tcactcacag gaaggaaaca
     3301 gcacctctct ccaggagggt cttcaggatc tcatccacac agccaccttg gtgacctgta
     3361 cttttctact ggcggtcatc ttctgcctgg gttcctatgg caacttcatt gtcttcttgt
     3421 ccttcttcga tccagccttc aggaaattca gaaccaactt tgatttcatg atcctgaacc
     3481 tgtccttctg tgacctcttc atttgtggag tgacagcccc catgttcacc tttgtgttat
     3541 tcttcagctc agtatcccgg atgctttctg cttcactttc catctcacca gttcaggctt
     3601 catcatcatg tctctgaaga cagtggcagt gatcgccctg caccggctcc ggatggtgtt
     3661 ggggaaacag cctaatcgca cggcctcctt tccctgcacc gtactcctca ccctgcttct
     3721 ctgggccacc agtttcaccc ttgccacctt ggctaccttg aaaaccagca agtcccacct
     3781 ctgtcttccc atgtccagtc tgattgctgg aaaagggaaa gccattttgt ctctctatgt
     3841 ggtcgacttc accttctgtg ttgctgtggt ctctgtctct tacatcatga ttgctcagac
     3901 cctgcggaag aacgctcaag tcagaaagtg cccccctgta atcacagtcg atgcttccag
     3961 accacagcct ttcatggggg tccctgtgca gggaggtgga gatcccatcc agtgtgccat
     4021 gccggctctg tataggaacc agaattacaa caaactgcag cacgttcaga cccgtggata
     4081 taccaagagt cccaaccaac tggtcacccc tgcagcaagc cgactccagc tcgtatcagc
     4141 catcaacctc tccactgcca aggattccaa agccgtggtc acctgtgtga tcattgtgct
     4201 gtcagtcctg gtgtgctgtc ttccactggg gatttccttg gtacaggtgg ttctctccag
     4261 caatgggagc ttcattcttt accagtttga attgtttgga tttactctta tatttttcaa
     4321 gtcaggatta aaccctttta tatattctcg gaacagtgca gggctgagaa ggaaagtgct
     4381 ctggtgcctc caatacatag gcctgggttt tttctgctgc aaacaaaaga ctcgacttcg
     4441 agccatggga aaagggaacc tcgaagtcaa cagaaacaaa tcctcccatc atgaaacaaa
     4501 ctctgcctac atgttatctc caaagccaca gaagaaattt gtggaccagg cttgtggccc
     4561 aagtcattca aaagaaagta tggtgagtcc caagatctct gctggacatc aacactgtgg
     4621 tcagagcagc tcgaccccca tcaacactcg gattgaacct tactacagca tctataacag
     4681 cagcccttcc caggaggaga gcagcccatg taacttacag ccagtaaact cttttggatt
     4741 tgccaattca tatattgcca tgcattatca caccactaat gacttagtgc aggaatatga
     4801 cagcacttca gccaagcaga ttccagtccc ctccgtttaa ctcgagccat ggaagcttga
     4861 tatctaactg actgaaccgg tggtaccgag gaattaattc gctgtctgcg agggccagct
     4921 gttggggtga gtactccctc tcaaaagcgg gcatgacttc tgcgctaaga ttgtcagttt
     4981 ccaaaaacga ggaggatttg atattcacct ggcccgcggt gatgcctttg agggtggccg
     5041 cgtccatctg gtcagaaaag acaatctttt tgttgtcaag cttgaggtgt ggcaggcttg
     5101 agatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     5161 ccactcccag gtccaactgc aggtcgagca tgcatctagg gcggccaatt ccgccgatcc
     5221 tggaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
     5281 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag acaatcaacc
     5341 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     5401 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     5461 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     5521 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     5581 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     5641 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     5701 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
     5761 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     5821 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     5881 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc gagctcggta
     5941 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     6001 gggggactgg aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc
     6061 taccacacac aaggctactt ccctgattga cagaactaca caccagggcc aggggtcaga
     6121 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taagatagaa
     6181 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat
     6241 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg
     6301 gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg
     6361 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     6421 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     6481 ttttagtcag tgtggaaaat ctctagcagg gccgctttaa acccgctgat cagcctcgac
     6541 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     6601 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     6661 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     6721 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     6781 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     6841 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     6901 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     6961 tcggggcatc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     7021 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     7081 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     7141 ccctatctcg gtctattctt ttgatttata agggattttg gggatttcgg cctattggtt
     7201 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     7261 ttagggtgtg gaaagtcccc aggctcccca ggcaggcaga agtatgcaaa gcatgcatct
     7321 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     7381 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     7441 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     7501 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     7561 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     7621 tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag
     7681 gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac
     7741 gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag
     7801 gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac
     7861 caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac
     7921 gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc
     7981 gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc
     8041 gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc
     8101 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     8161 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     8221 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     8281 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     8341 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     8401 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     8461 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     8521 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     8581 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     8641 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     8701 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     8761 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     8821 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     8881 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8941 ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag
     9001 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     9061 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     9121 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     9181 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     9241 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     9301 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     9361 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     9421 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     9481 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     9541 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     9601 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     9661 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     9721 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     9781 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     9841 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     9901 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     9961 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    10021 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    10081 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    10141 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    10201 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    10261 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    10321 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    10381 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    10441 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    10501 tttccccgaa aagtgccacc tgacgtc
//
