LOCUS       Exported                8714 bp ds-DNA     circular SYN 27-MAY-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8714)
  AUTHORS   Cody Kime
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 19, 2025 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8714
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     36..57
                     /label=Amp-pro-AS (11-16)
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F (7-72)
                     /note="F1 origin, forward primer"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     728..744
                     /label=M13F (5-10)
     primer_bind     812..831
                     /label=BII
                     /label=BII(R)
     repeat_region   join(874..886,918..919,921..936)
                     /label=5'TR
     misc_feature    887..917
                     /label=31bp spacer
     repeat_region   887..917
                     /label=5'TR
     enhancer        1113..1343
                     /label=Chicken beta-globin core insulator
                     /label=core insul(1)
     promoter        1371..1611
                     /label=hU6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     primer_bind     1374..1396
                     /label=hU6-seq-F (3-67)
     primer_bind     1397..1422
                     /label=hU6-S27 (2-30)
     primer_bind     complement(1541..1566)
                     /label=hU6-AS171 (2-31)
     primer_bind     1598..1620
                     /label=hU6-S228 (17-28)
     misc_feature    1621..1639
                     /label=Add Spacer Sequence Here
                     /note="The BbsI stuffer sequence has at least 3-nt 
                     mismatches to the human genome (GRCh38.p14 reference)."
     misc_feature    1622..1627
                     /label=BbsI-R
     misc_feature    1632..1637
                     /label=BbsI-F
     primer_bind     1639..1674
                     /label=MM BsmBI Uni F
     intron          1640..1734
                     /label=CR3
                     /note="Adamson et al., 2016 (PMID: 27984733)"
     primer_bind     complement(1745..1768)
                     /label=hU6-CR3-R
     promoter        1759..2072
                     /label=mU6 promoter
                     /note="Common feature added by Mo Mandegar"
     primer_bind     1873..1895
                     /label=mU6-S115 (2-14)
     protein_bind    1953..1986
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     protein_bind    1953..1986
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     primer_bind     2056..2127
                     /label=MM BsmBI Uni F
     misc_feature    2063..2066
                     /label=CACC
     misc_feature    2074..2092
                     /label=Add Spacer Sequence Here
                     /note="The Esp3I stuffer sequence has at least 6-nt 
                     mismatches to the human genome (GRCh38.p14 reference)."
     misc_feature    2075..2080
                     /label=Esp3I-R
     misc_feature    2086..2091
                     /label=Esp3I-F
     misc_feature    2093..2185
                     /label=modified stem loop
                     /note="Termination signal = 7 Ts at the end.Common feature 
                     added by Mo Mandegar
                     Modification refer to 
                     https://doi.org/10.1016/j.cell.2013.12.001"
     misc_feature    2093..2096
                     /label=GTTT
     terminator      2249..2259
                     /label=3X STOP
                     /note="Common feature added by Mo Mandegar"
     enhancer        2330..2633
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        2635..3882
                     /label=CAG
                     /note="Common feature added by Mo Mandegar"
     promoter        2635..2912
                     /label=chicken beta-actin promoter
     primer_bind     3855..3876
                     /label=CAG-F (5-18)
     intron          3890..3980
                     /label=Mini-Intron
                     /note="Mini Intron = Rabbit Globin Intron
                     Common feature added by Mo Mandegar"
     misc_feature    3986..3995
                     /label=Kozak+ATG
                     /note="Common feature added by Mo Mandegar
                     
                     Note: Designating ""cgccaccATG"" as the Kozak+ATG in a 
                     number of constructs. This is the most conserved consensues
                     sequence. Works well in HEK and iPS cells.
                     
                     From Wikipedia Dec 2013:
                     The Kozak consensus sequence, Kozak consensus or Kozak 
                     sequence, is a sequence which occurs on eukaryotic mRNA and
                     has the consensus (gcc)gccRccAUGG. The Kozak consensus 
                     sequence plays a major role in the initiation of the 
                     translation process.[1] The sequence was named after the 
                     person who brought it to prominence, Marilyn Kozak."
     CDS             3996..4016
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4020..4040
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4044..4064
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    4073
                     /label=Mu
                     /note="Mutated (silent) this to remove the BstXI from 
                     Original Seq
                     Still codes for a Serine
                     
                     Mo Mandegar"
     CDS             4080..4796
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from GFP (Lam et al., 2012)"
                     /label=Clover
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(4125..4146)
                     /label=EGFP-N (11-78)
                     /note="EGFP, reverse primer"
     primer_bind     4343..4364
                     /label=EGFP-S264 (5-28)
     primer_bind     complement(4437..4458)
                     /label=EGFP-AS357 (5-34)
     primer_bind     4733..4754
                     /label=EGFP-C (5-36)
                     /note="EGFP, forward primer"
     CDS             4797..4850
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    4811
                     /label=Synonymous mutation (T to C) to delete a BbsI site
     CDS             4851..5273
                     /codon_start=1
                     /product="Bacillus subtilis Blasticidin resistance gene"
                     /label=BSR
                     /label=Bsd
                     /note="Common feature added by Mo Mandegar"
                     /translation="MKTFNISQQDLELVEVATEKITMLYEDNKHHVGAAIRTKTGEIIS
                     AVHIEAYIGRVTVCAEAIAIGSAVSNGQKDFDTIVAVRHPYSDEVDRSIRVVSPCGMCR
                     ELISDYAPDCFVLIEMNGKLVKTTIEELIPLKYTRN"
     misc_feature    4856
                     /label=Synonymous mutation (G to A) to delete a BbsI site
     polyA_signal    5286..5407
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5323..5342)
                     /label=SV40pA-R (2-72)
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5590..5611)
                     /label=PB-3prime-AS (6-17)
     enhancer        complement(5665..5895)
                     /label=Chicken beta-globin core insulator
                     /label=core insul(1)
     repeat_region   6170..6204
                     /label=3'TR
     primer_bind     complement(6695..6711)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6695..6711)
                     /label=M13R (5-11)
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6708..6730)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6719..6735
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(6723..6742)
                     /label=pLac
                     /label=pLac(F)
     promoter        complement(6743..6773)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6788..6809
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    6918^6919
                     /label=2-bp (CC) deletion to disrupt a SapI site
     rep_origin      complement(7095..7683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7175..7194)
                     /label=pBR322ori-F (10-49)
                     /note="pBR322 origin, forward primer"
     CDS             complement(7854..8714)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8303..8325
                     /label=Amp-AS390 (2-52)
     primer_bind     complement(8474..8497)
                     /label=Amp-S218 (2-51)
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggcccgat gaggcccttg gagggcctat
     1381 ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa
     1441 ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat
     1501 ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg
     1561 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg
     1621 cgtcttcgaa tgaagacacg tttcagagct aagcacaaga gtgcatagca agttgaaata
     1681 aggctagtcc gtttacaact tgaaaaagtg gcacccgagt cgggtgcttt ttttgctcga
     1741 atctacactc agctcgagga tccgacgcgc catctctagg cccgcgccgg ccccctcgca
     1801 cggacttgtg ggagaagctc ggctactccc ctgccccggt taatttgcat ataatatttc
     1861 ctagtaacta tagaggctta atgtgcgata aaagacagat aatctgttct ttttaatact
     1921 agctacattt tacatgatag gcttggattt ctataacttc gtatagcata cattatacga
     1981 agttataaac agcacaaaag gaaactcacc ctaactgtaa agtaattgtg tgttttgaga
     2041 ctataagtat cccttggaga accaccttgt tggggagacg cgttacgtct ccgtttaaga
     2101 gctatgctgg aaacagcata gcaagtttaa ataaggctag tccgttatca acttgaaaaa
     2161 gtggcaccga gtcggtgctt tttttctcga gtactaggat ccattaggcg gccgcgtgga
     2221 taaccgtatt accgccatgc atcccgggtg actgactgag cggccgcact agttattaat
     2281 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     2341 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     2401 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     2461 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     2521 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     2581 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg
     2641 tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt
     2701 atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcgcg
     2761 cgccaggcgg ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg
     2821 cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc
     2881 ggccctataa aaagcgaagc gcgcggcggg cggggagtcg ctgcgacgct gccttcgccc
     2941 cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact
     3001 cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc gcttggttta
     3061 atgacggctt gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc gggagggccc
     3121 tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc
     3181 gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc
     3241 gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt gcgggggggg
     3301 ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt
     3361 gggcgcgtcg gtcgggctgc aaccccccct gcacccccct ccccgagttg ctgagcacgg
     3421 cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg
     3481 ggggtggcgg caggtggggg tgccgggcgg ggcggggccg cctcgggccg gggagggctc
     3541 gggggagggg cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag
     3601 ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg
     3661 tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg
     3721 gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg
     3781 tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg
     3841 acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa
     3901 ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct
     3961 gtctcatcat tttggcaaag aattccgcca ccatgccaaa aaagaagaga aaggtagatc
     4021 caaaaaagaa gagaaaggta gatccaaaaa agaagagaaa ggtacacgtg tcaacgcgta
     4081 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     4141 gcgacgtaaa cggccacaag ttcagcgtcc gcggcgaggg cgagggcgat gccaccaacg
     4201 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     4261 tcgtgaccac cttcggctac ggcgtggcct gcttcagccg ctaccccgac cacatgaagc
     4321 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatctctt
     4381 tcaaggacga cggtacctac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     4441 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     4501 agctggagta caacttcaac agccacaacg tctatatcac ggccgacaag cagaagaacg
     4561 gcatcaaggc taacttcaag atccgccaca acgttgagga cggcagcgtg cagctcgccg
     4621 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     4681 acctgagcca tcagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     4741 tgctggagtt cgtgaccgcc gccgggatta cacatggcat ggacgagctg tacaaggagg
     4801 gcagaggaag ccttctaaca tgcggtgacg tggaggagaa tcccggccct atgaaaacct
     4861 tcaacatctc ccagcaggat ctagaattag tagaagtagc gacagagaag attacaatgc
     4921 tttatgagga taataaacat catgtgggag cggcaattcg tacgaaaaca ggagaaatca
     4981 tttcggcagt acatattgaa gcgtatatag gacgagtaac tgtttgtgca gaagccattg
     5041 cgattggtag tgcagtttcg aatggacaaa aggattttga cacgattgta gctgttagac
     5101 acccttattc tgacgaagta gatagaagta ttcgagtggt aagtccttgt ggtatgtgta
     5161 gggagttgat ttcagactat gcaccagatt gttttgtgtt aatagaaatg aatggcaagt
     5221 tagtcaaaac tacgattgaa gaactcattc cactcaaata tacccgaaat taagttaact
     5281 aactaaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     5341 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     5401 tatcttatca tgtctggaat tgactcaaat gatgtcaatt agtctatcag aagctcatct
     5461 ggtctccctt ccgggggaca agacatccct gtttaatatt taaacagcag tgttcccaaa
     5521 ctgggttctt atatcccttg ctctggtcaa ccaggttgca gggtttcctg tcctcacagg
     5581 aacgaagtcc ctaaagaaac agtggcagcc aggtttagcc ccggaattga ctggattcct
     5641 tttttagggc ccattggtat ggctttttcc ccgtatcccc ccaggtgtct gcaggctcaa
     5701 agagcagcga gaagcgttca gaggaaagcg atcccgtgcc accttccccg tgcccgggct
     5761 gtccccgcac gctgccggct cggggatgcg gggggagcgc cggaccggag cggagccccg
     5821 ggcggctcgc tgctgccccc tagcggggga gggacgtaat tacatccctg ggggctttgg
     5881 gggggggctg tccctgatat ctataacaag aaaatatata tataataagt tatcacgtaa
     5941 gtagaacatg aaataacaat ataattatcg tatgagttaa atcttaaaag tcacgtaaaa
     6001 gataatcatg cgtcattttg actcacgcgg tcgttatagt tcaaaatcag tgacacttac
     6061 cgcattgaca agcacgcctc acgggagctc caagcggcga ctgagatgtc ctaaatgcac
     6121 agcgacggat tcgcgctatt tagaaagaga gagcaatatt tcaagaatgc atgcgtcaat
     6181 tttacgcaga ctatctttct agggttaatc tagctgcatc aggatcatat cgtcgggtct
     6241 tttttccggc tcagtcatcg cccaagctgg cgctatctgg gcatcgggga ggaagaagcc
     6301 cgtgcctttt cccgcgaggt tgaagcggca tggaaagagt ttgccgagga tgactgctgc
     6361 tgcattgacg ttgagcgaaa acgcacgttt accatgatga ttcgggaagg tgtggccatg
     6421 cacgccttta acggtgaact gttcgttcag gccacctggg ataccagttc gtcgcggctt
     6481 ttccggacac agttccggat ggtcagcccg aagcgcatca gcaacccgaa caataccggc
     6541 gacagccgga actgccgtgc cggtgtgcag attaatgaca gcggtgcggc gctgggatat
     6601 tacgtcagcg aggacgggta tcctggctgg atgccgcaga aatggacatg gataccccgt
     6661 gagttacccg gcgggcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     6721 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     6781 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     6841 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     6901 tgcgtattgg gcgctcttgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     6961 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     7021 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     7081 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     7141 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     7201 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     7261 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     7321 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     7381 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     7441 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     7501 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     7561 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     7621 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     7681 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     7741 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     7801 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     7861 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     7921 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     7981 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     8041 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     8101 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     8161 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     8221 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     8281 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     8341 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     8401 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     8461 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     8521 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     8581 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     8641 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     8701 tgttgaatac tcat
//
