LOCUS       Exported                8460 bp ds-DNA     circular SYN 25-MAR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8460)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 25, 2025 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8460
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(72..93)
                     /label=Amp-pro-AS (11-16)
     primer_bind     complement(73..94)
                     /label=pCS2-BB-S1 (7-57)
     CDS             129..989
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     346..369
                     /label=Amp-S218 (2-51)
     primer_bind     complement(518..540)
                     /label=Amp-AS390 (2-52)
     primer_bind     524..545
                     /label=pCS2-BB-AS2 (7-59)
     primer_bind     1084..1105
                     /label=pCS2-BB-AS3 (7-60)
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1355..1377
                     /label=ColE1ori-S196 (2-44)
     primer_bind     complement(1525..1548)
                     /label=ColE1ori-AS366 (2-45)
     primer_bind     1649..1668
                     /label=pBR322ori-F (10-49)
     primer_bind     1903..1924
                     /label=pCS2-BB-AS5 (7-62)
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2072..2102
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13R (5-11)
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    complement(2224..2464)
                     /label=ITR-L
     misc_feature    2235..2262
                     /label=External DR
     misc_feature    2434..2459
                     /label=Internal DR
     enhancer        2481..2860
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2862..3139
                     /label=chicken beta-actin promoter
     primer_bind     4080..4101
                     /label=CAG-F (5-18)
     intron          4115..4205
                     /label=Mini-Intron
                     /note="Mini Intron = Rabbit Globin Intron
                     Common feature added by Mo Mandegar"
     misc_signal     4212..5086
                     /label=SeqVer (TRE F)
                     /note="Common feature added by Mo Mandegar"
     misc_signal     4212..4221
                     /label=SeqVer (SeqVer (AAVR,TRE F, SV40 F)
     misc_feature    4222..4231
                     /label=Kozak+ATG
     CDS             4235..4255
                     /codon_start=1
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4259..4279
                     /codon_start=1
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4283..4303
                     /codon_start=1
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4319..5013
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGH"
     primer_bind     4702..4722
                     /label=mKate2-S384 (15-23)
     CDS             5030..5086
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    5074..5088
                     /label=Homology sequence for NEBuilder
     CDS             5090..6004
                     /codon_start=1
                     /label=Human NANOG CDS
                     /translation="SVDPACPQSLPCFEASDCKESSPMPVICGPEENYPSLQMSSAEMP
                     HTETVSPLPSSMDLLIQDSPDSSTSPKGKQPTSAEKSVAKKEDKVPVKKQKTRTVFSST
                     QLCVLNDRFQRQKYLSLQQMQELSNILNLSYKQVKTWFQNQRMKSKRWQKNNWPKNSNG
                     VTQKASAPTYPSLYSSYHQGCLVNPTGNLPMWSNQTWNNSTWSNQTQNIQSWSNHSWNT
                     QTWCTQSWNNQAWNSPFYNCGEESLQSCMQFQPNSPASDLEAALEAAGEGLNVIQQTTR
                     YFSTPQTMDLFLNYSMNMQPEDV"
     primer_bind     5464..5487
                     /label=hNANOG-594F (YM96-28)
     primer_bind     5558..5577
                     /label=hNANOG-688F (YM97-1)
     primer_bind     5590..5609
                     /label=hNANOG-CDS-S501
     misc_feature    6005..6019
                     /label=Homology sequence for NEBuilder
     misc_feature    6028..6614
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     6422..6441
                     /label=IRES-F (5-38)
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6615..7214
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     polyA_signal    7301..7525
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     7302..7325
                     /label=bGHpA-F (2-19)
     primer_bind     complement(7369..7390)
                     /label=bGHpA-AS1 (6-20)
     misc_feature    7552..7788
                     /label=ITR-R
     misc_feature    7553..7578
                     /label=Internal DR
     misc_feature    7755..7777
                     /label=External DR
     promoter        complement(7817..7835)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7845..7862)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7845..7861)
                     /label=M13F (5-10)
     primer_bind     complement(7854..7876)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8003..8458
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     8300..8321
                     /label=F1ori-F (7-72)
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctccac cgcggtggcg
     2221 gccggatccc tatacagttg aagtcggaag tttacataca cttaagttgg agtcattaaa
     2281 actcgttttt caactactcc acaaatttct tgttaacaaa caatagtttt ggcaagtcag
     2341 ttaggacatc tactttgtgc atgacacaag tcatttttcc aacaattgtt tacagacaga
     2401 ttatttcact tataattcac tgtatcacaa ttccagtggg tcagaagttt acatacacta
     2461 agttgctagc ggcgcgcctc gacattgatt attgactagt tattaatagt aatcaattac
     2521 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     2581 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     2641 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     2701 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     2761 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     2821 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt
     2881 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt
     2941 ttaattattt tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc caggcggggc
     3001 ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag ccaatcagag
     3061 cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa
     3121 gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc
     3181 cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg
     3241 gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc
     3301 ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag
     3361 cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct
     3421 gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg
     3481 cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa
     3541 aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg
     3601 gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc
     3661 ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt
     3721 gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aggggcgcgg
     3781 cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg
     3841 gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa atctgtgcgg agccgaaatc
     3901 tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag
     3961 gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct
     4021 ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg
     4081 gttcggcttc tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt
     4141 cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg
     4201 caaagaattc ccttaaggtt acgccaccat ggtgccaaag aagaagagga aagtggaccc
     4261 caagaaaaaa agaaaggttg atccaaaaaa gaaaagaaaa gtacacgtgt caacgcgtat
     4321 ggtgagcgag ctgattaagg agaacatgca catgaagctg tacatggagg gcaccgtgaa
     4381 caaccaccac ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac
     4441 catgagaatc aaggcggtcg agggcggccc tctccccttc gccttcgaca tcctggctac
     4501 cagcttcatg tacggcagca aaaccttcat caaccacacc cagggcatcc ccgacttctt
     4561 taagcagtcc ttccccgagg gcttcacatg ggagagagtc accacatacg aagacggggg
     4621 cgtgctgacc gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa
     4681 gatcagaggg gtgaacttcc catccaacgg ccctgtgatg cagaagaaaa cactcggctg
     4741 ggaggcctcc accgagaccc tgtaccccgc tgacggcggc ctggaaggca gagccgacat
     4801 ggccctgaag ctcgtgggcg ggggccacct gatctgcaac ttgaagacca catacagatc
     4861 caagaaaccc gctaagaacc tcaagatgcc cggcgtctac tatgtggaca gaagactgga
     4921 aagaatcaag gaggccgaca aagagaccta cgtcgagcag cacgaggtgg ctgtggccag
     4981 atactgcgac ctccctagca aactggggca caggtacaag ggatctggcg ccacgaactt
     5041 ctctctgtta aaacaagcag gagacgtgga agaaaatccc ggtccgatga gtgtggatcc
     5101 agcttgtccc caaagcttgc cttgctttga agcatccgac tgtaaagaat cttcacctat
     5161 gcctgtgatt tgtgggcctg aagaaaacta tccatccttg caaatgtctt ctgctgagat
     5221 gcctcacacg gagactgtct ctcctcttcc ttcctccatg gatctgctta ttcaggacag
     5281 ccctgattct tccaccagtc ccaaaggcaa acaacccact tctgcagaga agagtgtcgc
     5341 aaaaaaggaa gacaaggtcc cggtcaagaa acagaagacc agaactgtgt tctcttccac
     5401 ccagctgtgt gtactcaatg atagatttca gagacagaaa tacctcagcc tccagcagat
     5461 gcaagaactc tccaacatcc tgaacctcag ctacaaacag gtgaagacct ggttccagaa
     5521 ccagagaatg aaatctaaga ggtggcagaa aaacaactgg ccgaagaata gcaatggtgt
     5581 gacgcagaag gcctcagcac ctacctaccc cagcctttac tcttcctacc accagggatg
     5641 cctggtgaac ccgactggga accttccaat gtggagcaac cagacctgga acaattcaac
     5701 ctggagcaac cagacccaga acatccagtc ctggagcaac cactcctgga acactcagac
     5761 ctggtgcacc caatcctgga acaatcaggc ctggaacagt cccttctata actgtggaga
     5821 ggaatctctg cagtcctgca tgcagttcca gccaaattct cctgccagtg acttggaggc
     5881 tgccttggaa gctgctgggg aaggccttaa tgtaatacag cagaccacta ggtattttag
     5941 tactccacaa accatggatt tattcctaaa ctactccatg aacatgcaac ctgaagacgt
     6001 gtgaatcggc cgcaattccg cccccccccc ctctccctcc ccccccccta acgttactgg
     6061 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
     6121 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
     6181 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     6241 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     6301 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
     6361 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
     6421 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
     6481 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
     6541 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata
     6601 atatggccac aaccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     6661 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     6721 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     6781 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     6841 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     6901 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     6961 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     7021 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     7081 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     7141 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     7201 agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac
     7261 cccatgcatc gatgatctag agctcgctga tcagcctcga ctgtgccttc tagttgccag
     7321 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     7381 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     7441 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     7501 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaacctgcag cgagtgtatg
     7561 taaacttctg acccactggg aatgtgatga aagaaataaa agctgaaatg aatcattctc
     7621 tctactatta ttctgatatt tcacattctt aaaataaagt ggtgatccta actgacctaa
     7681 gacagggaat ttttactagg attaaatgtc aggaattgtg aaaaagtgag tttaaatgta
     7741 tttggctaag gtgtatgtaa acttccgact tcaactgtat agggatcctc gagggggggc
     7801 ccggtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     7861 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     7921 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     7981 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     8041 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     8101 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     8161 ctccctttag ggttccgatt tagtgcttga cggcacctcg accccaaaaa acttgattag
     8221 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     8281 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     8341 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     8401 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
//
