LOCUS       Exported               11485 bp ds-DNA     circular SYN 29-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11485)
  AUTHORS   Cody Kime
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, May 29, 2025 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11485
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     35..56
                     /label=pCS2-BB-S1 (7-57)
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F (7-63)
                     /note="F1 origin, forward primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     812..831
                     /label=BII
                     /label=BII(R)
     repeat_region   join(874..886,918..919,921..936)
                     /label=5'TR
     misc_feature    887..917
                     /label=31bp spacer
     repeat_region   887..917
                     /label=5'TR
     enhancer        1113..1343
                     /label=Chicken beta-globin core insulator
                     /label=core insul(1)
     misc_signal     1389..1798
                     /label=SeqVer (SeqVer (AAVR,TRE F, SV40 F)
     promoter        1413..1788
                     /label=TRE3G
                     /note="Common feature added by Mo Mandegar"
     primer_bind     1704..1725
                     /label=TRE3G-F (3-1)
     misc_signal     1755..2884
                     /label=SeqVer (TRE F)
                     /note="Common feature added by Mo Mandegar"
     primer_bind     1784..1822
                     /label=TP53-102622-S
     misc_feature    1784..1798
                     /label=NEBuilder 15-bp sequence (Left)
     primer_bind     1799..1837
                     /label=dN40-TP53-100322-S
     CDS             1818..2882
                     /codon_start=1
                     /gene="Homo sapiens TP53"
                     /product="human tumor suppressor protein that is often 
                     mutated in cancers"
                     /label=p53 CDS
                     /note="responds to cellular stresses to regulate the 
                     expression of target genes"
                     /translation="MDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPRVAPAPAAPTPAA
                     PAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPV
                     QLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLR
                     VEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSS
                     GNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKK
                     KPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSR
                     HKKLMFKTEGPDSD"
     misc_feature    1818..1820
                     /label=Alternative start codon for p47 (del 1-40aa)
     misc_feature    1915
                     /label=SNP (P33R)
                     /note="The C to G SNP makes the coding amino acid change 
                     from proline (P) to arginine (R). SNP rs1042522 (p.P33R) is
                     common in all world populations.
                     PMID: 33257846"
     primer_bind     2448..2469
                     /label=TP53-S890 (15-52)
     primer_bind     complement(2513..2540)
                     /label=TP53-CDS-AS813 (16-14)
     primer_bind     2526..2553
                     /label=TP53-CDS-S826 (16-13)
     primer_bind     complement(2724..2744)
                     /label=TP53-AS1166 (16-32)
     primer_bind     complement(2859..2897)
                     /label=TP53-102622-AS
     misc_feature    2883..2897
                     /label=NEBuilder 15-bp sequence (Right)
     misc_signal     complement(2892..2897)
                     /label=SeqVer (SV40 F)
                     /note="Common feature added by Mo Mandegar"
     misc_signal     2892..2897
                     /label=SeqVer (SeqVer (AAVR,TRE F, SV40 F)
     misc_signal     complement(2910..3669)
                     /label=SeqVer (SV40 F)
                     /note="Common feature added by Mo Mandegar"
     misc_signal     2910..3669
                     /label=SeqVer (SeqVer (AAVR,TRE F, SV40 F)
     misc_feature    2916..3504
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2969..2989)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3387..3398)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3537..3667
                     /label=SV40polyA
                     /label=SV40 pA
                     /note="Common feature added/edited by Mo Mandegar
                     Slightly longer sequence than the archieved SnapGene 
                     Version (131bp vs 122bp)"
     primer_bind     complement(3561..3586)
                     /label=SV40pA-R (2-63)
     misc_signal     3670..3672
                     /label=SeqVer (SeqVer (AAVR,TRE F, SV40 F)
     misc_signal     complement(3670..3672)
                     /label=SeqVer (SV40 F)
                     /note="Common feature added by Mo Mandegar"
     enhancer        3737..4040
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        4042..5289
                     /label=CAG
                     /note="Common feature added by Mo Mandegar"
     promoter        4042..4319
                     /label=chicken beta-actin promoter
     primer_bind     5262..5283
                     /label=CAG-F (5-9)
     intron          5297..5387
                     /label=Mini-Intron
                     /note="Mini Intron = Rabbit Globin Intron
                     Common feature added by Mo Mandegar"
     misc_signal     5394..5403
                     /label=SeqVer (TRE F)
                     /note="Common feature added by Mo Mandegar"
     misc_signal     5394..5403
                     /label=SeqVer (SeqVer (AAVR,TRE F, SV40 F)
     misc_signal     5404..6283
                     /label=SeqVer (TRE F)
                     /note="Common feature added by Mo Mandegar"
     misc_feature    5404..5413
                     /label=Kozak+ATG
     CDS             5417..5437
                     /codon_start=1
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5441..5461
                     /codon_start=1
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5465..5485
                     /codon_start=1
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5501..6217
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from GFP (Lam et al., 2012)"
                     /label=Clover
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(5546..5567)
                     /label=EGFP-N (11-78)
                     /note="EGFP, reverse primer"
     primer_bind     5764..5785
                     /label=EGFP-S264 (5-19)
     primer_bind     complement(5858..5879)
                     /label=EGFP-AS357 (5-25)
     primer_bind     6154..6175
                     /label=EGFP-C (5-27)
                     /note="EGFP, forward primer"
     CDS             6227..6283
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    6284..7030
                     /label=rtTA
                     /label=rtTA3G
                     /note="Common feature added by Mo Mandegar"
     primer_bind     complement(6400..6421)
                     /label=rtTA_BC-AS117 (15-46)
     primer_bind     6947..6968
                     /label=rtTA_BC-S664 (6-3)
     misc_feature    7047..7633
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     7441..7460
                     /label=IRES-F (5-38)
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7634..8032
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="Aspergilus terreus blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    8076..8300
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     8077..8100
                     /label=bGHpA-F (2-19)
     primer_bind     complement(8144..8165)
                     /label=bGHpA-AS1 (6-29)
     primer_bind     complement(8359..8380)
                     /label=PB-3prime-AS (6-17)
     enhancer        complement(8434..8664)
                     /label=Chicken beta-globin core insulator
                     /label=core insul(1)
     repeat_region   8939..8973
                     /label=3'TR
     primer_bind     complement(9464..9480)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9464..9480)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9477..9499)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9488..9504
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(9492..9511)
                     /label=pLac
                     /label=pLac(F)
     promoter        complement(9512..9542)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9557..9578
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9690..9711)
                     /label=pCS2-BB-AS5 (7-62)
     rep_origin      complement(9866..10454)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10066..10089
                     /label=ColE1ori-AS366 (2-45)
     primer_bind     complement(10237..10259)
                     /label=ColE1ori-S196 (2-44)
     primer_bind     complement(10509..10530)
                     /label=pCS2-BB-AS3 (7-60)
     CDS             complement(10625..11485)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11069..11090)
                     /label=pCS2-BB-AS2 (7-59)
     primer_bind     11074..11096
                     /label=Amp-AS390 (2-43)
     primer_bind     complement(11245..11268)
                     /label=Amp-S218 (2-42)
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggcccgat gaggcccttt cgtcttcact
     1381 cgaggatcgc tgatatctcc accccacagt ggtttactcc ctatcagtga tagagaacgt
     1441 atgaagagtt tactccctat cagtgataga gaacgtatgc agactttact ccctatcagt
     1501 gatagagaac gtataaggag tttactccct atcagtgata gagaacgtat gaccagttta
     1561 ctccctatca gtgatagaga acgtatctac agtttactcc ctatcagtga tagagaacgt
     1621 atatccagtt tactccctat cagtgataga gaacgtataa gctttaggcg tgtacggtgg
     1681 gcgcctataa aagcagagct cgtttagtga accgtcagat cgcctggagc aattccacaa
     1741 cacttttgtc ttataccaac tttccgtacc acttcctacc ctcgtaaact taaggttagg
     1801 tggtacggga attcaccatg gatgatttga tgctgtcccc ggacgatatt gaacaatggt
     1861 tcactgaaga cccaggtcca gatgaagctc ccagaatgcc agaggctgct ccccgcgtgg
     1921 cccctgcacc agcagctcct acaccggcgg cccctgcacc agccccctcc tggcccctgt
     1981 catcttctgt cccttcccag aaaacctacc agggcagcta cggtttccgt ctgggcttct
     2041 tgcattctgg gacagccaag tctgtgactt gcacgtactc ccctgccctc aacaagatgt
     2101 tttgccaact ggccaagacc tgccctgtgc agctgtgggt tgattccaca cccccgcccg
     2161 gcacccgcgt ccgcgccatg gccatctaca agcagtcaca gcacatgacg gaggttgtga
     2221 ggcgctgccc ccaccatgag cgctgctcag atagcgatgg tctggcccct cctcagcatc
     2281 ttatccgagt ggaaggaaat ttgcgtgtgg agtatttgga tgacagaaac acttttcgac
     2341 atagtgtggt ggtgccctat gagccgcctg aggttggctc tgactgtacc accatccact
     2401 acaactacat gtgtaacagt tcctgcatgg gcggcatgaa ccggaggccc atcctcacca
     2461 tcatcacact ggaagactcc agtggtaatc tactgggacg gaacagcttt gaggtgcgtg
     2521 tttgtgcctg tcctgggaga gaccggcgca cagaggaaga gaatctccgc aagaaagggg
     2581 agcctcacca cgagctgccc ccagggagca ctaagcgagc actgcccaac aacaccagct
     2641 cctctcccca gccaaagaag aaaccactgg atggagaata tttcaccctt cagatccgtg
     2701 ggcgtgagcg cttcgagatg ttccgagagc tgaatgaggc cttggaactc aaggatgccc
     2761 aggctgggaa ggagccaggg gggagcaggg ctcactccag ccacctgaag tccaaaaagg
     2821 gtcagtctac ctcccgccat aaaaaactca tgttcaagac agaagggcct gactcagact
     2881 gataagatat cgtatacgat ccattaggcg cggccaatca acctctggat tacaaaattt
     2941 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     3001 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     3061 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     3121 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     3181 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     3241 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     3301 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
     3361 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     3421 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     3481 tctccctttg ggccgcctcc ccgcaccggt aaaggccgca atcgatcgcc caccccaact
     3541 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     3601 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     3661 atgtctgtat acgcactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     3721 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     3781 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     3841 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     3901 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     3961 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     4021 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
     4081 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
     4141 tgggggcggg gggggggggg gggcgcgcgc caggcggggc ggggcggggc gaggggcggg
     4201 gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
     4261 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
     4321 ggagtcgctg cgacgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
     4381 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
     4441 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
     4501 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
     4561 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
     4621 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     4681 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     4741 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     4801 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     4861 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     4921 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     4981 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     5041 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     5101 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     5161 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     5221 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     5281 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     5341 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcccttaagg
     5401 ttacgccacc atggtgccaa agaagaagag gaaagtggac cccaagaaaa aaagaaaggt
     5461 tgatccaaaa aagaaaagaa aagtacacgt gtcaacgcgt atggtgagca agggcgagga
     5521 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     5581 gttcagcgtc cgcggcgagg gcgagggcga tgccaccaac ggcaagctga ccctgaagtt
     5641 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta
     5701 cggcgtggcc tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     5761 cgccatgccc gaaggctacg tccaggagcg caccatctct ttcaaggacg acggtaccta
     5821 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     5881 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaacttcaa
     5941 cagccacaac gtctatatca cggccgacaa gcagaagaac ggcatcaagg ctaacttcaa
     6001 gatccgccac aacgttgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     6061 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagcc atcagtccgc
     6121 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     6181 cgccgggatt acacatggca tggacgagct gtacaaggga tctggcgcca cgaacttctc
     6241 tctgttaaaa caagcaggag acgtggaaga aaatcccggt ccgatgagcc ggctggacaa
     6301 gagcaaagtg atcaacagcg ccctggaact gctgaacggc gtgggcatcg agggcctgac
     6361 cacccggaag ctggcccaga aactgggcgt ggaacagccc accctgtact ggcacgtgaa
     6421 gaacaagcgg gccctgctgg acgccctgcc catcgagatg ctggaccggc accacaccca
     6481 cagctgccct ctggaaggcg agagctggca ggacttcctg cggaacaacg ccaagagcta
     6541 cagatgcgcc ctgctgagcc accgggacgg cgccaaagtg cacctgggca ccagacccac
     6601 cgagaagcag tacgagacac tggaaaacca gctggccttc ctgtgccagc agggcttcag
     6661 cctggaaaac gccctgtacg ccctgagcgc cgtgggccac ttcaccctgg gctgcgtgct
     6721 ggaagaacag gaacaccagg tcgccaaaga ggaaagagag acacccacca ccgacagcat
     6781 gccccccctg ctgaagcagg ccatcgagct gttcgacaga cagggcgccg agcccgcctt
     6841 cctgttcggc ctggaactga tcatctgcgg cctcgagaag cagctgaagt gcgagagcgg
     6901 cggacccacc gacgccctgg acgacttcga cctggacatg ctgcccgccg atgccctgga
     6961 tgattttgat ctggatatgc tccctgctga cgctctcgat gacttcgatc tcgatatgct
     7021 gccaggctaa gttagatcta attccgcccc tctccctccc ccccccctaa cgttactggc
     7081 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     7141 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     7201 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     7261 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     7321 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     7381 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     7441 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     7501 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
     7561 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
     7621 tatggccaca accatggcca agcctttgtc tcaagaagaa tccaccctca ttgaaagagc
     7681 aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca gcgcagctct
     7741 ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg
     7801 tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat
     7861 cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt gccgacaggt
     7921 gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg gacagccgac
     7981 ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggct aagtcgagcc
     8041 tctagagtta tctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     8101 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     8161 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     8221 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     8281 ggatgcggtg ggctctatgg cttctgaggc ggaagtcaac caggttgcag ggtttcctgt
     8341 cctcacagga acgaagtccc taaagaaaca gtggcagcca ggtttagccc cggaattgac
     8401 tggattcctt ttttagggcc cattggtatg gctttttccc cgtatccccc caggtgtctg
     8461 caggctcaaa gagcagcgag aagcgttcag aggaaagcga tcccgtgcca ccttccccgt
     8521 gcccgggctg tccccgcacg ctgccggctc ggggatgcgg ggggagcgcc ggaccggagc
     8581 ggagccccgg gcggctcgct gctgccccct agcgggggag ggacgtaatt acatccctgg
     8641 gggctttggg ggggggctgt ccctgatatc tataacaaga aaatatatat ataataagtt
     8701 atcacgtaag tagaacatga aataacaata taattatcgt atgagttaaa tcttaaaagt
     8761 cacgtaaaag ataatcatgc gtcattttga ctcacgcggt cgttatagtt caaaatcagt
     8821 gacacttacc gcattgacaa gcacgcctca cgggagctcc aagcggcgac tgagatgtcc
     8881 taaatgcaca gcgacggatt cgcgctattt agaaagagag agcaatattt caagaatgca
     8941 tgcgtcaatt ttacgcagac tatctttcta gggttaatct agctgcatca ggatcatatc
     9001 gtcgggtctt ttttccggct cagtcatcgc ccaagctggc gctatctggg catcggggag
     9061 gaagaagccc gtgccttttc ccgcgaggtt gaagcggcat ggaaagagtt tgccgaggat
     9121 gactgctgct gcattgacgt tgagcgaaaa cgcacgttta ccatgatgat tcgggaaggt
     9181 gtggccatgc acgcctttaa cggtgaactg ttcgttcagg ccacctggga taccagttcg
     9241 tcgcggcttt tccggacaca gttccggatg gtcagcccga agcgcatcag caacccgaac
     9301 aataccggcg acagccggaa ctgccgtgcc ggtgtgcaga ttaatgacag cggtgcggcg
     9361 ctgggatatt acgtcagcga ggacgggtat cctggctgga tgccgcagaa atggacatgg
     9421 ataccccgtg agttacccgg cgggcgcgct tggcgtaatc atggtcatag ctgtttcctg
     9481 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     9541 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     9601 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     9661 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     9721 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     9781 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     9841 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     9901 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     9961 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
    10021 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
    10081 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
    10141 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
    10201 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
    10261 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
    10321 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
    10381 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    10441 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    10501 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
    10561 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
    10621 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
    10681 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
    10741 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
    10801 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
    10861 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
    10921 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    10981 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    11041 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    11101 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    11161 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    11221 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    11281 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    11341 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    11401 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    11461 cgacacggaa atgttgaata ctcat
//
