LOCUS       Exported                4260 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF-15NLS      4260 bp    DNA   circular            
            10/10/2011

            DEFINITION  Current Name: pF-15NLS
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..121
                     /label=CMVd1
     promoter        132..151
                     /label=T7 RNA Polymerase promoter  (-17 to +3)
     promoter        156..175
                     /label=SP6 RNA polymerase promoter (-17 to +3)
     misc_feature    182..189
                     /label=SgfI
                     /note="Used in Flexi Vector Cloning"
     CDS             213..548
                     /label=Barnase
     misc_feature    568..573
                     /label=EcoICR I
     misc_feature    573..617
                     /label=linker
     misc_feature    588..608
                     /label=TEV protease recognition sequence
     misc_feature    618..1508
                     /label=Halotag C terminal
                     /note=" Feature found by back translation of amino acid 
                     feature definition."
     transit_peptide 1518..1538
                     /label=NLS
     misc_feature    1542..1562
                     /label=NLS
                     /note="NLS"
     misc_feature    1566..1586
                     /label=NLS
                     /note="NLS"
     polyA_signal    1720..1941
                     /label=SV40 polyA signal
     CDS             2202..3062
                     /label=AmpR
                     /note="Removed RE site from standard beta-lactamase gene"
     rep_origin      3217..3253
                     /direction=RIGHT
                     /label=ori
                     /note="The actual origin is the first base."
ORIGIN
        1 ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga cgcaaatggg
       61 cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat
      121 cactagaagc ttaatacgac tcactatagg gtacgattta ggtgacacta tagaataagg
      181 agcgatcgcc atggaataag taaggaatcc acatggcaca ggttatcaac acgtttgacg
      241 gggttgcgga ttatcttcag acatatcata agctacctga taattacatt acaaaatcag
      301 aagcacaagc cctcggctgg gtggcatcaa aagggaacct tgcagacgtc gctccgggga
      361 aaagcatcgg cggagacatc ttctcaaaca gggaaggcaa actcccgggc aaaagcggac
      421 gaacatggcg tgaagcggat attaactata catcaggctt cagaaattca gaccggattc
      481 tttactcaag cgactggctg atttacaaaa caacggacca ttatcagacc tttacaaaaa
      541 tcagataatg tttaatgacc ccgtgtcgag ctctcgagcc aaccactgag gatctgtact
      601 ttcagagcga taacgatgga tccgaaatcg gtactggctt tccattcgac ccccattatg
      661 tggaagtcct gggcgagcgc atgcactacg tcgatgttgg tccgcgcgat ggcacccctg
      721 tgctgttcct gcacggtaac ccgacctcct cctacgtgtg gcgcaacatc atcccgcatg
      781 ttgcaccgac ccatcgctgc attgctccag acctgatcgg tatgggcaaa tccgacaaac
      841 cagacctggg ttatttcttc gacgaccacg tccgcttcat ggatgccttc atcgaagccc
      901 tgggtctgga agaggtcgtc ctggtcattc acgactgggg ctccgctctg ggtttccact
      961 gggccaagcg caatccagag cgcgtcaaag gtattgcatt tatggagttc atccgcccta
     1021 tcccgacctg ggacgaatgg ccagaatttg cccgcgagac cttccaggcc ttccgcacca
     1081 ccgacgtcgg ccgcaagctg atcatcgatc agaacgtttt tatcgagggt acgctgccga
     1141 tgggtgtcgt ccgcccgctg actgaagtcg agatggacca ttaccgcgag ccgttcctga
     1201 atcctgttga ccgcgagcca ctgtggcgct tcccaaacga gctgccaatc gccggtgagc
     1261 cagcgaacat cgtcgcgctg gtcgaagaat acatggactg gctgcaccag tcccctgtcc
     1321 cgaagctgct gttctggggc accccaggcg ttctgatccc accggccgaa gccgctcgcc
     1381 tggccaaaag cctgcctaac tgcaaggctg tggacatcgg cccgggtctg aatctgctgc
     1441 aagaagacaa cccggacctg atcggcagcg agatcgcgcg ctggctgtct actctggaga
     1501 tttccggagc cggcgatcca aaaaagaaga gaaaggtaga tccaaaaaag aagagaaagg
     1561 tagatccaaa aaagaagaga aaggtataat agaattctag agtcgacctg caggcatgca
     1621 agctgatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
     1681 gcaataacta gcataacccc ttggggcggc cgcttcgagc agacatgata agatacattg
     1741 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     1801 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     1861 attgcattca ttttatgttt caggttcagg gggagatgtg ggaggttttt ttaagcaagt
     1921 aaaacctcta caaatgtggt aaaatcgaat tctaatggat cctctttgcg cttgcgtttt
     1981 cccttgtcca gatagcccag tagctgacat tcatccgggg tcagcaccgt ttctgcggac
     2041 tggctttcta cgtaatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     2101 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     2161 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     2221 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     2281 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     2341 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     2401 tgagcacttt caaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     2461 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     2521 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     2581 tgagtgataa cactgcggcc aacttacttc tgacaactat cggaggaccg aaggagctaa
     2641 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     2701 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     2761 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     2821 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     2881 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     2941 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     3001 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     3061 aattcgaaat gaccgaccaa gcgacgccca accggtatca gctcactcaa aggcggtaat
     3121 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     3181 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     3241 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     3301 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     3361 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     3421 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     3481 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     3541 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     3601 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     3661 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     3721 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     3781 gattacgcgc agaaaaaaag gatttcaaga agatcctttg atcttttcta cggggtctga
     3841 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     3901 cttcacctag atccttttat agtccggaaa tacaggaacg cacgctggat ggcccttcgc
     3961 tgggatggtg aaaccatgaa aaatggcagc ttcagtggat taagtggggg taatgtggcc
     4021 tgtaccctct ggttgcatag gtattcatac ggttaaaatt tatcaggcgc gattgcggca
     4081 gtttttcggg tggtttgttg ccatttttac ctgtctgctg ccgtgatcgc gctgaacgcg
     4141 ttttagcggt gcgtacaatt aagggattat ggtaaatcca cttactgtct gccctcgtag
     4201 ccatcgagat aaaccgcagt actccggcca cgatgcgtcc ggcgtagagg atcgagatct
//
