LOCUS       Exported                5996 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       DQ904459                6010 bp    DNA     circular SYN 
            26-SEP-2006

            DEFINITION  Firefly luciferase reporter vector 
            pGL4.27[luc2P/minP/Hygro],

                        complete sequence.

            ACCESSION   DQ904459

            VERSION     DQ904459.1  GI:115342887

            KEYWORDS    .

            SOURCE      Firefly luciferase reporter vector 
            pGL4.27[luc2P/minP/Hygro]

              ORGANISM  Firefly luciferase reporter vector 
            pGL4.27[luc2P/minP/Hygro]

                        other sequences; artificial sequences; vectors.

            REFERENCE   1  (bases 1 to 6010)

              AUTHORS   Swanson,B., Kopish,K. and McNamara,B.

              TITLE     pGL4 Luciferase reporter Vectors Technical Manual, 
            TM259

              JOURNAL   Unpublished

            REFERENCE   2  (bases 1 to 6010)

              AUTHORS   Swanson,B., Kopish,K. and McNamara,B.

              TITLE     Direct Submission

              JOURNAL   Submitted (16-AUG-2006) Scientific Communications, 
            Promega

                        Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, 
            USA
FEATURES             Location/Qualifiers
     source          1..5996
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    20..50
                     /label=STAT4-RE
                     /note="STAT4-RE"
     promoter        64..94
                     /label=minimal promoter
                     /note="minimal promoter"
     gene            127..1902
                     /label=luc2P
     polyA_signal    1942..2163
                     /label=SV40 poly(A) signal
                     /note="SV40 poly(A) signal"
     misc_feature    2211..2629
                     /label=SV40 promoter
                     /note="SV40 promoter"
     CDS             2654..3691
                     /codon_start=1
                     /label=hygromycin resistance
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     rep_origin      4087
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="ColE1-derived plasmid replication origin"
     gene            complement(4878..5738)
                     /label=AmpR
     polyA_signal    5843..5996
                     /label=poly(A) pause site
                     /note="poly(A) pause site"
ORIGIN
        1 ggcctaactg gccggtacct acaacagcct gatttccccg aaatgacggc aagcttagac
       61 actagagggt atataatgga agctcgactt ccagcttggc aatccggtac tgttggtaaa
      121 gccaccatgg aagatgccaa aaacattaag aagggcccag cgccattcta cccactcgaa
      181 gacgggaccg ccggcgagca gctgcacaaa gccatgaagc gctacgccct ggtgcccggc
      241 accatcgcct ttaccgacgc acatatcgag gtggacatta cctacgccga gtacttcgag
      301 atgagcgttc ggctggcaga agctatgaag cgctatgggc tgaatacaaa ccatcggatc
      361 gtggtgtgca gcgagaatag cttgcagttc ttcatgcccg tgttgggtgc cctgttcatc
      421 ggtgtggctg tggccccagc taacgacatc tacaacgagc gcgagctgct gaacagcatg
      481 ggcatcagcc agcccaccgt cgtattcgtg agcaagaaag ggctgcaaaa gatcctcaac
      541 gtgcaaaaga agctaccgat catacaaaag atcatcatca tggatagcaa gaccgactac
      601 cagggcttcc aaagcatgta caccttcgtg acttcccatt tgccacccgg cttcaacgag
      661 tacgacttcg tgcccgagag cttcgaccgg gacaaaacca tcgccctgat catgaacagt
      721 agtggcagta ccggattgcc caagggcgta gccctaccgc accgcaccgc ttgtgtccga
      781 ttcagtcatg cccgcgaccc catcttcggc aaccagatca tccccgacac cgctatcctc
      841 agcgtggtgc catttcacca cggcttcggc atgttcacca cgctgggcta cttgatctgc
      901 ggctttcggg tcgtgctcat gtaccgcttc gaggaggagc tattcttgcg cagcttgcaa
      961 gactataaga ttcaatctgc cctgctggtg cccacactat ttagcttctt cgctaagagc
     1021 actctcatcg acaagtacga cctaagcaac ttgcacgaga tcgccagcgg cggggcgccg
     1081 ctcagcaagg aggtaggtga ggccgtggcc aaacgcttcc acctaccagg catccgccag
     1141 ggctacggcc tgacagaaac aaccagcgcc attctgatca cccccgaagg ggacgacaag
     1201 cctggcgcag taggcaaggt ggtgcccttc ttcgaggcta aggtggtgga cttggacacc
     1261 ggtaagacac tgggtgtgaa ccagcgcggc gagctgtgcg tccgtggccc catgatcatg
     1321 agcggctacg ttaacaaccc cgaggctaca aacgctctca tcgacaagga cggctggctg
     1381 cacagcggcg acatcgccta ctgggacgag gacgagcact tcttcatcgt ggaccggctg
     1441 aagagcctga tcaaatacaa gggctaccag gtagccccag ccgaactgga gagcatcctg
     1501 ctgcaacacc ccaacatctt cgacgccggg gtcgccggcc tgcccgacga cgatgccggc
     1561 gagctgcccg ccgcagtcgt cgtgctggaa cacggtaaaa ccatgaccga gaaggagatc
     1621 gtggactatg tggccagcca ggttacaacc gccaagaagc tgcgcggtgg tgttgtgttc
     1681 gtggacgagg tgcctaaagg actgaccggc aagttggacg cccgcaagat ccgcgagatt
     1741 ctcattaagg ccaagaaggg cggcaagatc gccgtgaatt ctcacggctt ccctcccgag
     1801 gtggaggagc aggccgccgg caccctgccc atgagctgcg cccaggagag cggcatggat
     1861 agacaccctg ctgcttgcgc cagcgccagg atcaacgtct aaggccgcga ctctagagtc
     1921 ggggcggccg gccgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca
     1981 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     2041 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     2101 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
     2161 gtaaaatcga taaggatccg tttgcgtatt gggcgctctt ccgctgatct gcgcagcacc
     2221 atggcctgaa ataacctctg aaagaggaac ttggttagct accttctgag gcggaaagaa
     2281 ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     2341 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     2401 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     2461 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     2521 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     2581 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcg attcttctga
     2641 cactagcgcc accatgaaga agcccgaact caccgctacc agcgttgaaa aatttctcat
     2701 cgagaagttc gacagtgtga gcgacctgat gcagttgtcg gagggcgaag agagccgagc
     2761 cttcagcttc gatgtcggcg gacgcggcta tgtactgcgg gtgaatagct gcgctgatgg
     2821 cttctacaaa gaccgctacg tgtaccgcca cttcgccagc gctgcactac ccatccccga
     2881 agtgttggac atcggcgagt tcagcgagag cctgacatac tgcatcagta gacgcgccca
     2941 aggcgttact ctccaagacc tccccgaaac agagctgcct gctgtgttac agcctgtcgc
     3001 cgaagctatg gatgctattg ccgccgccga cctcagtcaa accagcggct tcggcccatt
     3061 cgggccccaa ggcatcggcc agtacacaac ctggcgggat ttcatttgcg ccattgctga
     3121 tccccatgtc taccactggc agaccgtgat ggacgacacc gtgtccgcca gcgtagctca
     3181 agccctggac gaactgatgc tgtgggccga agactgtccc gaggtgcgcc acctcgtcca
     3241 tgccgacttc ggcagcaaca acgtcctgac cgacaacggc cgcatcaccg ccgtaatcga
     3301 ctggtccgaa gctatgttcg gggacagtca gtacgaggtg gccaacatct tcttctggcg
     3361 gccctggctg gcttgcatgg agcagcagac tcgctacttc gagcgccggc atcccgagct
     3421 ggccggcagc cctcgtctgc gagcctacat gctgcgcatc ggcctggatc agctctacca
     3481 gagcctcgtg gacggcaact tcgacgatgc tgcctgggct caaggccgct gcgatgccat
     3541 cgtccgcagc ggggccggca ccgtcggtcg cacacaaatc gctcgccgga gcgcagccgt
     3601 atggaccgac ggctgcgtcg aggtgctggc cgacagcggc aaccgccggc ccagtacacg
     3661 accgcgcgct aaggaggtag gtcgagttta aactctagaa ccggtcatgg ccgcaataaa
     3721 atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgttcgaac tagatgctgt
     3781 cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg
     3841 actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg
     3901 gcagcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     3961 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     4021 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     4081 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     4141 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     4201 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     4261 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     4321 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     4381 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     4441 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     4501 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     4561 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     4621 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     4681 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     4741 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     4801 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagcg gccgcaaatg
     4861 ctaaaccact gcagtggtta ccagtgcttg atcagtgagg caccgatctc agcgatctgc
     4921 ctatttcgtt cgtccatagt ggcctgactc cccgtcgtgt agatcactac gattcgtgag
     4981 ggcttaccat caggccccag cgcagcaatg atgccgcgag agccgcgttc accggccccc
     5041 gatttgtcag caatgaacca gccagcaggg agggccgagc gaagaagtgg tcctgctact
     5101 ttgtccgcct ccatccagtc tatgagctgc tgtcgtgatg ctagagtaag aagttcgcca
     5161 gtgagtagtt tccgaagagt tgtggccatt gctactggca tcgtggtatc acgctcgtcg
     5221 ttcggtatgg cttcgttcaa ctctggttcc cagcggtcaa gccgggtcac atgatcaccc
     5281 atattatgaa gaaatgcagt cagctcctta gggcctccga tcgttgtcag aagtaagttg
     5341 gccgcggtgt tgtcgctcat ggtaatggca gcactacaca attctcttac cgtcatgcca
     5401 tccgtaagat gcttttccgt gaccggcgag tactcaacca agtcgttttg tgagtagtgt
     5461 atacggcgac caagctgctc ttgcccggcg tctatacggg acaacaccgc gccacatagc
     5521 agtactttga aagtgctcat catcgggaat cgttcttcgg ggcggaaaga ctcaaggatc
     5581 ttgccgctat tgagatccag ttcgatatag cccactcttg cacccagttg atcttcagca
     5641 tcttttactt tcaccagcgt ttcggggtgt gcaaaaacag gcaagcaaaa tgccgcaaag
     5701 aagggaatga gtgcgacacg aaaatgttgg atgctcatac tcgtcctttt tcaatattat
     5761 tgaagcattt atcagggtta ctagtacgtc tctcaaggat aagtaagtaa tattaaggta
     5821 cgggaggtat tggacaggcc gcaataaaat atctttattt tcattacatc tgtgtgttgg
     5881 ttttttgtgt gaatcgatag tactaacata cgctctccat caaaacaaaa cgaaacaaaa
     5941 caaactagca aaataggctg tccccagtgc aagtgcaggt gccagaacat ttctct
//
