LOCUS       Exported                6432 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       DQ904459                6010 bp    DNA     circular SYN 
            26-SEP-2006

            DEFINITION  Firefly luciferase reporter vector 
            pGL4.27[luc2P/minP/Hygro],

                        complete sequence.

            ACCESSION   DQ904459

            VERSION     DQ904459.1  GI:115342887

            KEYWORDS    .

            SOURCE      Firefly luciferase reporter vector 
            pGL4.27[luc2P/minP/Hygro]

              ORGANISM  Firefly luciferase reporter vector 
            pGL4.27[luc2P/minP/Hygro]

                        other sequences; artificial sequences; vectors.

            REFERENCE   1  (bases 1 to 6010)

              AUTHORS   Swanson,B., Kopish,K. and McNamara,B.

              TITLE     pGL4 Luciferase reporter Vectors Technical Manual, 
            TM259

              JOURNAL   Unpublished

            REFERENCE   2  (bases 1 to 6010)

              AUTHORS   Swanson,B., Kopish,K. and McNamara,B.

              TITLE     Direct Submission

              JOURNAL   Submitted (16-AUG-2006) Scientific Communications, 
            Promega

                        Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, 
            USA
FEATURES             Location/Qualifiers
     source          1..6432
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    20..486
                     /label=CYP2B6 promoter
                     /note="CYP2B6 promoter"
     promoter        500..530
                     /label=minimal promoter
                     /note="minimal promoter"
     gene            563..2338
                     /label=luc2P
     polyA_signal    2378..2599
                     /label=SV40 poly(A) signal
                     /note="SV40 poly(A) signal"
     misc_feature    2647..3065
                     /label=SV40 promoter
                     /note="SV40 promoter"
     CDS             3090..4127
                     /codon_start=1
                     /label=hygromycin resistance
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     rep_origin      4523
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="ColE1-derived plasmid replication origin"
     gene            complement(5314..6174)
                     /label=AmpR
     polyA_signal    6279..6432
                     /label=poly(A) pause site
                     /note="poly(A) pause site"
ORIGIN
        1 ggcctaactg gccggtaccc tttctccatc cacaaaatgg gggtgaaaac aattactaca
       61 tcaaagggtg acaactaagt gaattactct attgaaagca cttcatgcct ccttatcagt
      121 gctccccaag ggtggaatga ggacccagag tacccgtatt aggccatact tgtgttgcta
      181 taaagactgt gccagattgc acaacacagc aggagacagg tcctggggtc aggaaagtcc
      241 atggtggcat ctttcctagg tcaggaaagt gtgggttgta ctttgttggt cctctcctcg
      301 tgggttgggg aagtatgggg tgcagctgct cctggcagct ttgactttca gcctcagata
      361 atgcattgcc tgtgtctaat ccacatggat gtggggtgac tcagccacct ccattccctg
      421 aatcagcatc ccttggttca ggaaagtcca tgctgccacc tcttcagggt caggaaagta
      481 cagtttaagc ttagacacta gagggtatat aatggaagct cgacttccag cttggcaatc
      541 cggtactgtt ggtaaagcca ccatggaaga tgccaaaaac attaagaagg gcccagcgcc
      601 attctaccca ctcgaagacg ggaccgccgg cgagcagctg cacaaagcca tgaagcgcta
      661 cgccctggtg cccggcacca tcgcctttac cgacgcacat atcgaggtgg acattaccta
      721 cgccgagtac ttcgagatga gcgttcggct ggcagaagct atgaagcgct atgggctgaa
      781 tacaaaccat cggatcgtgg tgtgcagcga gaatagcttg cagttcttca tgcccgtgtt
      841 gggtgccctg ttcatcggtg tggctgtggc cccagctaac gacatctaca acgagcgcga
      901 gctgctgaac agcatgggca tcagccagcc caccgtcgta ttcgtgagca agaaagggct
      961 gcaaaagatc ctcaacgtgc aaaagaagct accgatcata caaaagatca tcatcatgga
     1021 tagcaagacc gactaccagg gcttccaaag catgtacacc ttcgtgactt cccatttgcc
     1081 acccggcttc aacgagtacg acttcgtgcc cgagagcttc gaccgggaca aaaccatcgc
     1141 cctgatcatg aacagtagtg gcagtaccgg attgcccaag ggcgtagccc taccgcaccg
     1201 caccgcttgt gtccgattca gtcatgcccg cgaccccatc ttcggcaacc agatcatccc
     1261 cgacaccgct atcctcagcg tggtgccatt tcaccacggc ttcggcatgt tcaccacgct
     1321 gggctacttg atctgcggct ttcgggtcgt gctcatgtac cgcttcgagg aggagctatt
     1381 cttgcgcagc ttgcaagact ataagattca atctgccctg ctggtgccca cactatttag
     1441 cttcttcgct aagagcactc tcatcgacaa gtacgaccta agcaacttgc acgagatcgc
     1501 cagcggcggg gcgccgctca gcaaggaggt aggtgaggcc gtggccaaac gcttccacct
     1561 accaggcatc cgccagggct acggcctgac agaaacaacc agcgccattc tgatcacccc
     1621 cgaaggggac gacaagcctg gcgcagtagg caaggtggtg cccttcttcg aggctaaggt
     1681 ggtggacttg gacaccggta agacactggg tgtgaaccag cgcggcgagc tgtgcgtccg
     1741 tggccccatg atcatgagcg gctacgttaa caaccccgag gctacaaacg ctctcatcga
     1801 caaggacggc tggctgcaca gcggcgacat cgcctactgg gacgaggacg agcacttctt
     1861 catcgtggac cggctgaaga gcctgatcaa atacaagggc taccaggtag ccccagccga
     1921 actggagagc atcctgctgc aacaccccaa catcttcgac gccggggtcg ccggcctgcc
     1981 cgacgacgat gccggcgagc tgcccgccgc agtcgtcgtg ctggaacacg gtaaaaccat
     2041 gaccgagaag gagatcgtgg actatgtggc cagccaggtt acaaccgcca agaagctgcg
     2101 cggtggtgtt gtgttcgtgg acgaggtgcc taaaggactg accggcaagt tggacgcccg
     2161 caagatccgc gagattctca ttaaggccaa gaagggcggc aagatcgccg tgaattctca
     2221 cggcttccct cccgaggtgg aggagcaggc cgccggcacc ctgcccatga gctgcgccca
     2281 ggagagcggc atggatagac accctgctgc ttgcgccagc gccaggatca acgtctaagg
     2341 ccgcgactct agagtcgggg cggccggccg cttcgagcag acatgataag atacattgat
     2401 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     2461 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     2521 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
     2581 aacctctaca aatgtggtaa aatcgataag gatccgtttg cgtattgggc gctcttccgc
     2641 tgatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttagctacct
     2701 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     2761 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     2821 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     2881 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     2941 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     3001 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     3061 agctcgattc ttctgacact agcgccacca tgaagaagcc cgaactcacc gctaccagcg
     3121 ttgaaaaatt tctcatcgag aagttcgaca gtgtgagcga cctgatgcag ttgtcggagg
     3181 gcgaagagag ccgagccttc agcttcgatg tcggcggacg cggctatgta ctgcgggtga
     3241 atagctgcgc tgatggcttc tacaaagacc gctacgtgta ccgccacttc gccagcgctg
     3301 cactacccat ccccgaagtg ttggacatcg gcgagttcag cgagagcctg acatactgca
     3361 tcagtagacg cgcccaaggc gttactctcc aagacctccc cgaaacagag ctgcctgctg
     3421 tgttacagcc tgtcgccgaa gctatggatg ctattgccgc cgccgacctc agtcaaacca
     3481 gcggcttcgg cccattcggg ccccaaggca tcggccagta cacaacctgg cgggatttca
     3541 tttgcgccat tgctgatccc catgtctacc actggcagac cgtgatggac gacaccgtgt
     3601 ccgccagcgt agctcaagcc ctggacgaac tgatgctgtg ggccgaagac tgtcccgagg
     3661 tgcgccacct cgtccatgcc gacttcggca gcaacaacgt cctgaccgac aacggccgca
     3721 tcaccgccgt aatcgactgg tccgaagcta tgttcgggga cagtcagtac gaggtggcca
     3781 acatcttctt ctggcggccc tggctggctt gcatggagca gcagactcgc tacttcgagc
     3841 gccggcatcc cgagctggcc ggcagccctc gtctgcgagc ctacatgctg cgcatcggcc
     3901 tggatcagct ctaccagagc ctcgtggacg gcaacttcga cgatgctgcc tgggctcaag
     3961 gccgctgcga tgccatcgtc cgcagcgggg ccggcaccgt cggtcgcaca caaatcgctc
     4021 gccggagcgc agccgtatgg accgacggct gcgtcgaggt gctggccgac agcggcaacc
     4081 gccggcccag tacacgaccg cgcgctaagg aggtaggtcg agtttaaact ctagaaccgg
     4141 tcatggccgc aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtgt
     4201 tcgaactaga tgctgtcgac cgatgccctt gagagccttc aacccagtca gctccttccg
     4261 gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact
     4321 cgtaggacag gtgccggcag cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     4381 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     4441 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     4501 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     4561 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     4621 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     4681 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     4741 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     4801 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     4861 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     4921 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     4981 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     5041 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     5101 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     5161 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     5221 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     5281 acagcggccg caaatgctaa accactgcag tggttaccag tgcttgatca gtgaggcacc
     5341 gatctcagcg atctgcctat ttcgttcgtc catagtggcc tgactccccg tcgtgtagat
     5401 cactacgatt cgtgagggct taccatcagg ccccagcgca gcaatgatgc cgcgagagcc
     5461 gcgttcaccg gcccccgatt tgtcagcaat gaaccagcca gcagggaggg ccgagcgaag
     5521 aagtggtcct gctactttgt ccgcctccat ccagtctatg agctgctgtc gtgatgctag
     5581 agtaagaagt tcgccagtga gtagtttccg aagagttgtg gccattgcta ctggcatcgt
     5641 ggtatcacgc tcgtcgttcg gtatggcttc gttcaactct ggttcccagc ggtcaagccg
     5701 ggtcacatga tcacccatat tatgaagaaa tgcagtcagc tccttagggc ctccgatcgt
     5761 tgtcagaagt aagttggccg cggtgttgtc gctcatggta atggcagcac tacacaattc
     5821 tcttaccgtc atgccatccg taagatgctt ttccgtgacc ggcgagtact caaccaagtc
     5881 gttttgtgag tagtgtatac ggcgaccaag ctgctcttgc ccggcgtcta tacgggacaa
     5941 caccgcgcca catagcagta ctttgaaagt gctcatcatc gggaatcgtt cttcggggcg
     6001 gaaagactca aggatcttgc cgctattgag atccagttcg atatagccca ctcttgcacc
     6061 cagttgatct tcagcatctt ttactttcac cagcgtttcg gggtgtgcaa aaacaggcaa
     6121 gcaaaatgcc gcaaagaagg gaatgagtgc gacacgaaaa tgttggatgc tcatactcgt
     6181 cctttttcaa tattattgaa gcatttatca gggttactag tacgtctctc aaggataagt
     6241 aagtaatatt aaggtacggg aggtattgga caggccgcaa taaaatatct ttattttcat
     6301 tacatctgtg tgttggtttt ttgtgtgaat cgatagtact aacatacgct ctccatcaaa
     6361 acaaaacgaa acaaaacaaa ctagcaaaat aggctgtccc cagtgcaagt gcaggtgcca
     6421 gaacatttct ct
//
