LOCUS       Exported                6245 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pFN31K(pF-Nn5K3)      5128 bp    DNA   circular         
              9/3/2015

            DEFINITION  Current Name: pFN31K (pF-Nn5K3) Old/Alternate 
            Name(s):pF-Nn5K3 (backbone)
FEATURES             Location/Qualifiers
     source          1..6245
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1599..3074
                     /label=SOS1_Cat_557_1049
     promoter        1..742
                     /label=CMV immediate-early enhancer/promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 RNA Polymerase promoter
     CDS             1065..1577
                     /label=NanoLuc
                     /note="N Terminal version. Renamed from 'N terminal 
                     NanoLuc' Feb 2015"
     CDS             1068..1577
                     /label=NanoLuc
                     /note="C Terminal version. Renamed from 'C terminal 
                     NanoLuc' Feb 2015"
     CDS             1068..1577
                     /label=NanoLuc
                     /note="C Terminal version. Renamed from 'C terminal 
                     NanoLuc' Feb 2015"
     misc_feature    1578..1589
                     /label=N Terminal NanoLuc linker
     misc_feature    1590..1597
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1599..3074
                     /label=SOS1 aa557-1040
                     /note="SOS1 aa557-1040"
     misc_feature    3075..3082
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    3079..3212
                     /label=Transfer Efficiency Cassette
                     /note="Feature changed from 
                     ""AAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAA
                     GCTGATCCGGCTGCTAACAAAGC"" at Jim Hartnet's request 
                     12/18/2007 to include the complete cassette."
     polyA_signal    3234..3455
                     /label=Sv40 late polyadenylation signal
     promoter        3554..3972
                     /label=SV40 enhancer and early promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     enhancer        complement(3627..3863)
                     /label=SV40 Enhancer
                     /note=" Feature was found in the reverse orientation."
     rep_origin      3870..3935
                     /direction=RIGHT
                     /label=Sv40 Min Ori
     promoter        3980..4046
                     /label=EM7 bacterial promoter
     CDS             4060..4854
                     /label=Neomycin phosphotransferase coding
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    4918..4966
                     /label=Synthetic PolyAdenylation Signal
     rep_origin      5202..5238
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     misc_feature    5909..6194
                     /label=Cer Region
                     /note="Prevents multimerization"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgct caccatggtc ttcacactcg
     1081 aagatttcgt tggggactgg cgacagacag ccggctacaa cctggaccaa gtccttgaac
     1141 agggaggtgt gtccagtttg tttcagaatc tcggggtgtc cgtaactccg atccaaagga
     1201 ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag
     1261 gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt taaggtggtg taccctgtgg
     1321 atgatcatca ctttaaggtg atcctgcact atggcacact ggtaatcgac ggggttacgc
     1381 cgaacatgat cgactatttc ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa
     1441 agatcactgt aacagggacc ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca
     1501 accccgacgg ctccctgctg ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt
     1561 gcgaacgcat tctggcgggc tcgagcggcg cgatcgccat gctacaggaa gagaaagagg
     1621 agcagatgag gctgcctagt gctgatgttt atagatttgc agagcctgac tctgaagaga
     1681 atattatatt tgaagagaac atgcagccca aggctggaat tccaattatc aaagcaggaa
     1741 ctgttattaa acttatagag aggcttacgt accatatgta cgcagatccc aattttgttc
     1801 ggacatttct tacaacatac agatcctttt gcaaacctca agaactactg agtcttataa
     1861 tagaaaggtt tgaaattcca gagcctgagc caacagaagc tgatcgcata gctatagaga
     1921 atggagatca acccttgagt gcagaactga aaagatttag aaaagaatat atacagcctg
     1981 tgcaactgcg agtattaaat gtatgtcggc actgggtaga gcaccacttc tatgattttg
     2041 aaagagatgc atatcttttg caacgaatgg aagaatttat tggaacagta agaggtaaag
     2101 caatgaaaaa atgggttgaa tccatcacta aaataatcca aaggaaaaaa attgcaagag
     2161 acaatggacc aggtcataat attacatttc agagttcacc tcccacagtt gagtggcata
     2221 taagcagacc tgggcacata gagacttttg acctgctcac cttacaccca atagaaattg
     2281 ctcgacaact cactttactt gaatcagatc tataccgagc tgtacagcca tcagaattag
     2341 ttggaagtgt gtggacaaaa gaagacaaag aaattaactc tcctaatctt ctgaaaatga
     2401 ttcgacatac caccaacctc actctgtggt ttgagaaatg tattgtagaa actgaaaatt
     2461 tagaagaaag agtagctgtg gtgagtcgaa ttattgagat tctacaagtc tttcaagagt
     2521 tgaacaactt taatggtgtc cttgaggttg tcagtgctat gaattcatca cctgtttaca
     2581 gactagacca cacatttgag caaataccaa gtcgccagaa gaaaatttta gaagaagctc
     2641 atgaattgag tgaagatcac tataagaaat atttggcaaa actcaggtct attaatccac
     2701 catgtgtgcc tttctttgga atttatctca ctaatatctt gaaaacagaa gaaggcaacc
     2761 ctgaggtcct aaaaagacat ggaaaagagc ttataaactt tagcaaaagg aggaaagtag
     2821 cagaaataac aggagagatc cagcagtacc aaaatcagcc ttactgttta cgagtagaat
     2881 cagatatcaa aaggttcttt gaaaacttga atccgatggg aaatagcatg gagaaggaat
     2941 ttacagatta tcttttcaac aaatccctag aaatagaacc acgaaaccct aagcctctcc
     3001 caagatttcc aaaaaaatat agctatcccc taaaatctcc tggtgttcgt ccatcaaacc
     3061 caagaccagg taccgtttaa acgaattcgg gctcggtacc cggggatcct ctagagtcga
     3121 cctgcaggca tgcaagctga tccggctgct aacaaagccc gaaaggaagc tgagttggct
     3181 gctgccaccg ctgagcaata actagcataa ccccttgggg cggccgcttc gagcagacat
     3241 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     3301 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     3361 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggaga tgtgggaggt
     3421 ttttttaagc aagtaaaacc tctacaaatg tggtaaaatc gaattttaac aaaatattaa
     3481 cgcttacaat ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     3541 catacgcgga tctgcgcagc accatggcct gaaataacct ctgaaagagg aacttggtta
     3601 ggtaccttct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag
     3661 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     3721 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     3781 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     3841 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     3901 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     3961 tgcaaaaagc ttaattaact gttgacaatt aatcatcggc atagtatatc ggcatagtat
     4021 aatacgacaa ggtgaggaac taaacccagg aggcagatca tgattgaaca agatggattg
     4081 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     4141 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     4201 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
     4261 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     4321 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     4381 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     4441 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     4501 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     4561 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
     4621 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     4681 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     4741 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     4801 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     4861 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga tggccgcaat
     4921 aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagcgata
     4981 aggatcctct ttgcgcttgc gttttccctt gtccagatag cccagtagct gacattcatc
     5041 cggggtcagc accgtttctg cggactggct ttctacccgg tatcagctca ctcaaaggcg
     5101 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     5161 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     5221 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     5281 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     5341 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     5401 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     5461 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     5521 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     5581 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     5641 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     5701 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     5761 cagcagatta cgcgcagaaa aaaaggattt caagaagatc ctttgatctt ttctacgggg
     5821 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     5881 aggatcttca cctagatcct tttatagtcc ggaaatacag gaacgcacgc tggatggccc
     5941 ttcgctggga tggtgaaacc atgaaaaatg gcagcttcag tggattaagt gggggtaatg
     6001 tggcctgtac cctctggttg cataggtatt catacggtta aaatttatca ggcgcgattg
     6061 cggcagtttt tcgggtggtt tgttgccatt tttacctgtc tgctgccgtg atcgcgctga
     6121 acgcgtttta gcggtgcgta caattaaggg attatggtaa atccacttac tgtctgccct
     6181 cgtagccatc gagataaacc gcagtactcc ggccacgatg cgtccggcgt agaggatcga
     6241 gatct
//
