LOCUS       Exported                6656 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6656
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..3679
                     /label=BFBRSG15
                     /note="BFBRSG15"
     misc_feature    complement(1..3672)
                     /label=BFBRSG16
                     /note="BFBRSG16"
     misc_feature    1..3672
                     /label=PCR #2 (pF-4B)
                     /note="PCR #2 (pF-4B)"
     misc_feature    1..217
                     /label=PCR #1 (pF-BiBiTsmC)
                     /note="PCR #1 (pF-BiBiTsmC)"
     misc_feature    complement(19..206)
                     /label=SV40\polyA\signal
     misc_feature    complement(218..259)
                     /label=MCS 2
     misc_feature    complement(297..304)
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    308..874
                     /label=KRas 4A isoform
                     /note="KRas 4A isoform"
     misc_feature    complement(876..883)
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    complement(884..895)
                     /label=linker
                     /note="linker"
     misc_feature    complement(896..1408)
                     /label=NanoLuc
                     /note="NanoLuc"
     misc_feature    1422..1448
                     /label=B globin leader
                     /note="B globin leader"
     misc_feature    complement(1449..1465)
                     /label=SP6 promoter
                     /note="SP6 promoter"
     misc_feature    complement(1468..1513)
                     /label=Minimal CMV Promoter
     misc_feature    1491..1513
                     /label=core
                     /note="core"
     promoter        1523..2113
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          2228..2360
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        2404..2423
                     /label=T7 promoter
     misc_feature    2427..2434
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    2436..2687
                     /label=cRaf RBD (51-134)
                     /note="cRaf RBD (51-134)"
     misc_feature    2694..2738
                     /label=HT7 Linker C terminal
     misc_feature    2739..3629
                     /label=HT7 tag C terminal
     misc_feature    3650..3655
                     /label=SbfI
                     /note="SbfI"
     misc_feature    3656..5824
                     /label=PCR #1 (pF-BiBiTsmC)
                     /note="PCR #1 (pF-BiBiTsmC)"
     polyA_signal    3707..3928
                     /label=Sv40 late polyadenylation signal
     promoter        3994..4060
                     /label=EM7 bacterial promoter
     CDS             4074..4868
                     /label=KanR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     misc_feature    4875..4912
                     /label=Untitled
     rep_origin      5023..5059
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     polyA_signal    complement(5741..5788)
                     /label=Synthetic PolyAdenylation Signal
     misc_feature    complement(5790..5824)
                     /label=BFBRSG14
                     /note="BFBRSG14"
     misc_feature    5800..6653
                     /label=PCR #2 (pF-4B)
                     /note="PCR #2 (pF-4B)"
     misc_feature    5800..5834
                     /label=BFBRSG13
                     /note="BFBRSG13"
     misc_feature    complement(5812..6210)
                     /label=BlastR
                     /note="BlastR"
     misc_feature    complement(6235..6653)
                     /label=SV40 promoter
                     /note="SV40 promoter"
     misc_feature    complement(6628..6653)
                     /label=BFBRSG16
                     /note="BFBRSG16"
     misc_feature    6645..6653
                     /label=BFBRSG15
                     /note="BFBRSG15"
     misc_feature    6654..6656
                     /label=PCR #1 (pF-BiBiTsmC)
                     /note="PCR #1 (pF-BiBiTsmC)"
     misc_feature    6654
                     /label=PCR #1 (pF-BiBiTsmC)
                     /note="PCR #1 (pF-BiBiTsmC)"
ORIGIN
        1 acgcgttaat taagacgtcg gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
       61 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
      121 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
      181 tacaaatgtg gtatggctga ttatgatccg cggccgcaat acgtcgacgt tatcagctga
      241 cttcgtacga gagcctaggt gtttctagag gatccccggg taccgagccc gaattcgttt
      301 aaacttacat tataatgcat tttttaattt tcacacagcc aggagtcttt tcttctttgc
      361 tgattttttt caatctgtat tgtcggatct ccctcaccaa tgtataaaaa gcatcctcca
      421 ctctctgtct tgtctttgct gatgtttcaa taaaaggaat tccataactt cttgctaagt
      481 cctgagcctg ttttgtgtct actgttctag aaggcaaatc acatttattt cctactagga
      541 ccataggtac atcttcagag tccttaactc ttttaatttg ttctctataa tggtgaatat
      601 cttcaaatga tttagtatta tttatggcaa atacacaaag aaagccctcc ccagtcctca
      661 tgtactggtc cctcattgca ctgtactcct cttgacctgc tgtgtcgaga atatccaaga
      721 gacaggtttc tccatcaatt actacttgct tcctgtagga atcctctatt gttggatcat
      781 attcgtccac aaaatgattc tgaattagct gtatcgtcaa ggcactcttg cctacgccac
      841 cagctccaac taccacaagt ttatattcag tcatggcgat cgcgccgctc gagcccgcca
      901 gaatgcgttc gcacagccgc cagccggtca ctccgttgat ggttactcgg aacagcaggg
      961 agccgtcggg gttgatcagg cgctcgtcga taattttgtt gccgttccac agggtccctg
     1021 ttacagtgat ctttttgccg tcgaacacgg cgatgccttc atacggccgt ccgaaatagt
     1081 cgatcatgtt cggcgtaacc ccgtcgatta ccagtgtgcc atagtgcagg atcaccttaa
     1141 agtgatgatc atccacaggg tacaccacct taaaaatttt ttcgatctgg cccatttggt
     1201 cgccgctcag accttcatac gggatgatga catggatgtc gatcttcagc ccattttcac
     1261 cgctcaggac aatcctttgg atcggagtta cggacacccc gagattctga aacaaactgg
     1321 acacacctcc ctgttcaagg acttggtcca ggttgtagcc ggctgtctgt cgccagtccc
     1381 caacgaaatc ttcgagtgtg aagaccatgg tgagcgctag ctgcaaaaag aacaagtagc
     1441 ttgtattcta tagtgtcacc taaatgggcg atctgacggt tcactaaacc agctctgctt
     1501 atataggcct ccctggcgcg ccactagtta ttaatagtaa tcaattacgg ggtcattagt
     1561 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     1621 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     1681 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     1741 agtacatcaa gtgtatcata tgccaagtcc gccccctatt gacgtcaatg acggtaaatg
     1801 gcccgcctgg cattatgccc agtacatgac cttacgggac tttcctactt ggcagtacat
     1861 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca ccaatgggcg
     1921 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
     1981 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaataacc ccgccccgtt
     2041 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt
     2101 gaaccgtcag atcactagaa gctttattgc ggtagtttat cacagttaaa ttgctaacgc
     2161 agtcagtgct tctgacacaa cagtctcgaa cttaagctgc agaagttggt cgtgaggcac
     2221 tgggcaggta agtatcaagg ttacaagaca ggtttaagga gaccaataga aactgggctt
     2281 gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta ctgacatcca
     2341 ctttgccttt ctctccacag gtgtccactc ccagttcaat tacagctctt aaggctagag
     2401 tattaatacg actcactata gggctagcga tcgccatgcc ttctaagaca agcaacacta
     2461 tccgtgtttt cttgccgaac aagcaaagaa cagtggtcaa tgtgcgaaat ggaatgagct
     2521 tgcatgactg ccttatgaaa gcactcaagg tgaggggcct gcaaccagag tgctgtgcag
     2581 tgttcagact tctccacgaa cacaaaggta aaaaagcacg cttagattgg aatactgatg
     2641 ctgcgtcttt gattggagaa gaacttcaag tagatttcct ggatcatgtt tctctcgagc
     2701 caaccactga ggatctgtac tttcagagcg ataacgatgg atccgaaatc ggtactggct
     2761 ttccattcga cccccattat gtggaagtcc tgggcgagcg catgcactac gtcgatgttg
     2821 gtccgcgcga tggcacccct gtgctgttcc tgcacggtaa cccgacctcc tcctacgtgt
     2881 ggcgcaacat catcccgcat gttgcaccga cccatcgctg cattgctcca gacctgatcg
     2941 gtatgggcaa atccgacaaa ccagacctgg gttatttctt cgacgaccac gtccgcttca
     3001 tggatgcctt catcgaagcc ctgggtctgg aagaggtcgt cctggtcatt cacgactggg
     3061 gctccgctct gggtttccac tgggccaagc gcaatccaga gcgcgtcaaa ggtattgcat
     3121 ttatggagtt catccgccct atcccgacct gggacgaatg gccagaattt gcccgcgaga
     3181 ccttccaggc cttccgcacc accgacgtcg gccgcaagct gatcatcgat cagaacgttt
     3241 ttatcgaggg tacgctgccg atgggtgtcg tccgcccgct gactgaagtc gagatggacc
     3301 attaccgcga gccgttcctg aatcctgttg accgcgagcc actgtggcgc ttcccaaacg
     3361 agctgccaat cgccggtgag ccagcgaaca tcgtcgcgct ggtcgaagaa tacatggact
     3421 ggctgcacca gtcccctgtc ccgaagctgc tgttctgggg caccccaggc gttctgatcc
     3481 caccggccga agccgctcgc ctggccaaaa gcctgcctaa ctgcaaggct gtggacatcg
     3541 gcccgggtct gaatctgctg caagaagaca acccggacct gatcggcagc gagatcgcgc
     3601 gctggctgtc tactctggag atttccggtt aatagaattc tagagtcgac ctgcaggcat
     3661 gcaagctgat ttggctgctg ccaccgctga gcaaggccgc ttcgagcaga catgataaga
     3721 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     3781 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     3841 aacaacaatt gcattcattt tatgtttcag gttcaggggg agatgtggga ggttttttta
     3901 agcaagtaaa acctctacaa atgtggtaaa atcttaatta agaattctaa tataacttcg
     3961 tatagcatac attatacgaa gttatggatc cactgttgac aattaatcat cggcatagta
     4021 tatcggcata gtataatacg acaaggtgag gaactaaacc caggaggcag atcatgattg
     4081 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     4141 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     4201 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
     4261 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     4321 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     4381 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     4441 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     4501 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     4561 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
     4621 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     4681 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     4741 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     4801 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     4861 tcttctgatt cgaaatgacc gaccaagcga cgcccaaccg gtatcagctc actcaaaggc
     4921 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     4981 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     5041 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     5101 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     5161 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     5221 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     5281 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     5341 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     5401 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     5461 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     5521 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     5581 gcagcagatt acgcgcagaa aaaaaggatt tcaagaagat cctttgatct tttctacggg
     5641 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     5701 aaggatcttc acctagatcc ttttatagtc cggaatcgtc acacaaaaaa ccaacacaca
     5761 gatgtaatga aaataaagat attttattgc ggccatcgtg atggctagag tttagccctc
     5821 ccacacataa ccagagggca gcaattcacg aatcccaact gccgtcggct gtccatcact
     5881 gtccttcact atggctttga tcccaggatg cagatcgaga agcacctgtc ggcaccgtcc
     5941 gcaggggctc aagatgcccc tgttctcatt tccgatcgcg acgatacaag tcaggttgcc
     6001 agctgccgca gcagcagcag tgcccagcac cacgagttct gcacaaggtc ccccagtaaa
     6061 atgatataca ttgacaccag tgaagatgcg gccgtcgcta gagagagctg cgctggcgac
     6121 gctgtagtct tcagagatgg ggatgctgtt gattgtagcc gttgctcttt caatgagggt
     6181 ggattcttct tgagacaaag gcttggccat ggtggcgcta gtgtcagaag aatcgagctt
     6241 tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc
     6301 cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg
     6361 ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc
     6421 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc
     6481 acacctggtt gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc
     6541 tggggacttt ccacacccta actgacacac attccacagc tggttctttc cgcctcagaa
     6601 ggtacctaac caagttcctc tttcagaggt tatttcaggc catggtgctg cgcaag
//
