LOCUS       Exported                8773 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF4A(CMV)      4123 bp    DNA   circular            
            1/3/2014

            DEFINITION  Current Name: pF4A (CMV)
FEATURES             Location/Qualifiers
     source          1..8773
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          301..3504
                     /label=PIK3CANM_006218
     misc_feature    complement(15..202)
                     /label=SV40\polyA\signal
     misc_feature    complement(206..255)
                     /label=MCS 2
     misc_feature    complement(293..300)
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    complement(301..3504)
                     /label=PI3KCA1
                     /note="PI3KCA1"
     misc_feature    complement(3506..3513)
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    complement(3514..3525)
                     /label=linker
                     /note="linker"
     misc_feature    complement(3526..4038)
                     /label=NanoLuc
                     /note="NanoLuc"
     misc_feature    4052..4078
                     /label=B globin leader
                     /note="B globin leader"
     misc_feature    complement(4079..4095)
                     /label=SP6 promoter
                     /note="SP6 promoter"
     misc_feature    complement(4098..4143)
                     /label=Minimal CMV Promoter 2
     misc_feature    4121..4143
                     /label=core
                     /note="core"
     promoter        4153..4707
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     promoter        4725..4791
                     /label=EM7 bacterial promoter
     CDS             4805..5464
                     /label=Chloramphenicol resistance
     rep_origin      5633..5669
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     polyA_signal    complement(6351..6398)
                     /label=Synthetic PolyAdenylation Signal
     CDS             complement(6422..7216)
                     /label=NeoR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     misc_recomb     complement(7230..7524)
                     /label=R4 aatB
                     /note="R4 aatB"
     promoter        complement(7595..8773)
                     /label=EF1a
                     /note="EF1a"
ORIGIN
        1 aagacgcgtg acgtcggcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
       61 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
      121 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
      181 aatgtggtat ggctgattat gatccgcggc cgcaatacgt cgacgttatc agctgacttc
      241 gtacgagagc ctaggtgttt ctagaggatc cccgggtacc gagcccgaat tcgtttaaac
      301 gttcaatgca tgctgtttaa ttgtgtggaa gatccaatcc atttttgttg tccagccacc
      361 atgatgtgca tcattcattt gtttcatgaa atactccaaa gcctcttgct cagttttatc
      421 taaggctagg gtctttcgaa tgtatgcaat gtcatcaaaa gattgtagtt ctggcattcc
      481 agagccaagc atcattgaga aaagatttat gaagagattg gcatgctgtc gaatagctag
      541 ataagccttg taacacatct cctgaaacct ctcaaattct cttgtctttg tgcattcttg
      601 ggctccttta ctaatcacta ttaagaaatc ctgtgtcaaa acaaatggca cacgttctcg
      661 tttataacca aattttttct tcttgtgatc caaaaagtgt ccaaaatcta tatgaaacag
      721 ttgtccatcg tctttcacca tgatgttact attgtgacga tctccaattc ccaaaatgaa
      781 ggtagctaca cagtatccag cacatgaacg tgtaaacagg tcaatggctg catcatatat
      841 ttctcctttg ttcttgtctt tgagccactg atgtagtgtg tggctgttga actgcagtgc
      901 acctttcaag ccgcctttgc actgaatttg cataatagtg tgagaatttc gcaccacctc
      961 aataagtccc acacagtcac cgattgacag acaaccataa ggtaacattc gaagatcaag
     1021 accttgattt tgccagatat tttccataat acgaataatt tgaagtgtta gcatatcttg
     1081 ccgtaaatca tccccatttt taaagatgat ctcattgttc tgaaacagta actctgacat
     1141 gatgtctggg ttctcccaat tcaaccacag tggccttttt gcagaggaca taattcgaca
     1201 ctcttcaagc ctgaggtttc ctagttgatg agcagggttt agaggagaca gaaagccctg
     1261 tagagcatcc atgaaatctg gtcgcctcat ttgctcaact aaaaacttca tctgtacctt
     1321 ttgtgtttca tccttcttct cctgtttgag aatgtcagtt aagttaatga gcttttccat
     1381 tgcctcgact tgcctattca ggtgcttcaa atacatccca catgcacgac aataggactc
     1441 caaaagcagg ccaaacctct ggctaactgt tttattgtgc atctcagatt ttaaatgcca
     1501 aaagaaaaag tgcccaatcc tttgattagt caatgctttc ttcagtaaaa atctcacaag
     1561 caagttatcc aaatattgtt catattttag gacctgtact agctgaatta aatactgaga
     1621 aagtttgtca tctgttaaat atttttccaa gcaccgaaca gcaaaacctc gaaccatagg
     1681 atctgggtaa ttacagtcca gaagttccat agcctgttca ggtttgattg gaggccaatc
     1741 ttttaccaag caatacatct gggctacttc atctctagaa ttccatttaa cagacagaag
     1801 caatttgggt agaatttcgg ggatagttac acaatagtgt ctgtgactcc atagaaaatc
     1861 tttctcctgc tcagtgattt cagagagagg atctcgtgta gaaattgctt tgagctgttc
     1921 tttgtcattt tcccttaatt cattgtctct agctagtctg ttactcagtc ctgcgtggga
     1981 atagctaaat cctgcttctc gggatacaga ccaattggca tgctcttcaa tcactgacat
     2041 atctgggaac tttaccacac tgctgaacca gtcaaactcc aactctaagc atggagtttc
     2101 tttatttgga tttgatccag taacaccaat agggttcagc aaatcttcta atccatgagg
     2161 tactggccaa agattcaaag ccatttttcc agatactaga gtgtctgtgt aatcaaacaa
     2221 gtttatattt ccccatgcca atggacagtg ttcctcttta gcaccctttc ggcctttaac
     2281 agagcaaatg gaaaggcaaa gtcgagcagc acgaggaaga tcaggaatgt atatatcata
     2341 attcagccat tcattccacc tgggattgga acaaggtact ctttgagtgt tcacattgtc
     2401 acataagggt tctcctccat ggtagatacc tgttcgaaca tagatcttat caatgtctcg
     2461 aatatttaca ttcacgtagg ttgcacaaag aatttttatt ctgagtgcac tatttataac
     2521 ccaaagggat tttgtagatg tttctccatt catatatggt gtagctgtgg aaatgcgtct
     2581 ggaataagat ggcattgtaa aacagtccat tggcagttga gaataaaggc tttctttagc
     2641 catcaacatc aaattgggca tcctcccaag cattatacag cttcttatat acttatactg
     2701 actcagagga tatttttcta ggaagtattc atcacatcca cacactttta aaatatactt
     2761 gccctgatat tctaaaacac agagttttag ttgttcagag gatagcaaca tacttcgagt
     2821 ttttttcctg attgcttcag caattacttg ttctggtaca cagtcatggt tgattttcag
     2881 agtatacttc tgcttgtcat tatttggaga aactattacc cagatcacca ctattatttg
     2941 ccctttatct aatttattat atatgtgctt tggcaattct ggtgaagatt ctacatttgg
     3001 aggatagaca tacattgctc tactatgagg tgaattgagg tccctaagat ccacagcttc
     3061 tttacaaacg ttcagaatat ttcttcggaa gtcctgtact tctggatctt taaccatatc
     3121 aaattcacac actggcatgc cgatagcaaa accaatttct cgattgagga tcttttcttc
     3181 acggttgcct actggttcaa ttacttttaa aaagggttga aaaagccgaa ggtcacaaag
     3241 tcgtcttgtt tcatcaaaaa attcttccct ttctgcttct tgagtaacac ttacgaaaat
     3301 gtaagaagat tcatcttgaa gaagttgatg gagggggtat tttcttgctt ctttaaatag
     3361 ttcatgcttt atggttatta atgtagcctc acggaggcat tctaaagtca ctatcattcc
     3421 atttggtagt aaacattcta ctaggattct tgggggcatc aagtggatgc cccacagttc
     3481 acctgatgat ggtcgtggag gcatggcgat cgcgccgctc gagcccgcca gaatgcgttc
     3541 gcacagccgc cagccggtca ctccgttgat ggttactcgg aacagcaggg agccgtcggg
     3601 gttgatcagg cgctcgtcga taattttgtt gccgttccac agggtccctg ttacagtgat
     3661 ctttttgccg tcgaacacgg cgatgccttc atacggccgt ccgaaatagt cgatcatgtt
     3721 cggcgtaacc ccgtcgatta ccagtgtgcc atagtgcagg atcaccttaa agtgatgatc
     3781 atccacaggg tacaccacct taaaaatttt ttcgatctgg cccatttggt cgccgctcag
     3841 accttcatac gggatgatga catggatgtc gatcttcagc ccattttcac cgctcaggac
     3901 aatcctttgg atcggagtta cggacacccc gagattctga aacaaactgg acacacctcc
     3961 ctgttcaagg acttggtcca ggttgtagcc ggctgtctgt cgccagtccc caacgaaatc
     4021 ttcgagtgtg aagaccatgg tgagcgctag ctgcaaaaag aacaagtagc ttgtattcta
     4081 tagtgtcacc taaatgggcg atctgacggt tcactaaacc agctctgctt atataggcct
     4141 ccctggcgcg ccactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     4201 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     4261 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     4321 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     4381 gtgtatcata tgccaagtcc gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     4441 cattatgccc agtacatgac cttacgggac tttcctactt ggcagtacat ctacgtatta
     4501 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca ccaatgggcg tggatagcgg
     4561 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     4621 caccaaaatc aacgggactt tccaaaatgt cgtaataacc ccgccccgtt gacgcaaatg
     4681 ggcggtaggc gtgtacggtg ggaggtcaaa tcgaattcta atactgttga caattaatca
     4741 tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac ccaggaggca
     4801 gatcatggag aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa
     4861 agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct
     4921 ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt
     4981 tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga
     5041 cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac
     5101 tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat
     5161 atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat
     5221 tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa
     5281 cgtggccaat atggacaact tcttcgcccc cgttttcact atgggcaaat attatacgca
     5341 aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgtct gtgatggctt
     5401 ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc
     5461 gtaagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaaccg gtatcagctc
     5521 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     5581 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     5641 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     5701 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     5761 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     5821 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     5881 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     5941 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     6001 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     6061 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     6121 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     6181 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatt tcaagaagat cctttgatct
     6241 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     6301 gattatcaaa aaggatcttc acctagatcc ttttatagtc cggaatcgtc acacaaaaaa
     6361 ccaacacaca gatgtaatga aaataaagat attttattgc ggccatcgtg atggctagag
     6421 ttcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga
     6481 taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca gcaatatcac
     6541 gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca cagtcgatga
     6601 atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg ccgtgggtca
     6661 cgacgagatc ctcgccgtcg ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg
     6721 cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct tccatccgag
     6781 tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa
     6841 gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca ggagcaaggt
     6901 gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt
     6961 cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc
     7021 gcgctgcctc gtcctgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa
     7081 ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt
     7141 gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc
     7201 catcttgttc aatcatggtg gcatcgattc ccggtcaaca tccagtacac ctccggcacc
     7261 accggcttcc ccaagggcgc cacgctctcc caccacaaca tcctcaacaa cggctactgg
     7321 gtgggccgca cggtcggcta cacggagcag gaccgggtgt gtctgccggt gcccttctac
     7381 cactgcttcg gcatggtcat gggcaacttg ggcgccacct cccacgccgc ctgcatcgtc
     7441 atcccggccc cgtccttcga gccggcggcg acgctggagg cggtgcagcg ggagaggtgc
     7501 acgtccctgt acggcgtccc cacgaagccg aattccacca cactggacta gatgcatgct
     7561 cgacagtgtg atggatatct gcagaattcg gctttcacga cacctgaaat ggaagaaaaa
     7621 aactttgaac cactgtctga ggcttgagaa tgaaccaaga tccaaactca aaaagggcaa
     7681 attccaagga gaattacatc aagtgccaag ctggcctaac ttcagtctcc acccactcag
     7741 tgtggggaaa ctccatcgca taaaacccct ccccccaacc taaagacgac gtactccaaa
     7801 agctcgagaa ctaatcgagg tgcctggacg gcgcccggta ctccgtggag tcacatgaag
     7861 cgacggctga ggacggaaag gcccttttcc tttgtgtggg tgactcaccc gcccgctctc
     7921 ccgagcgccg cgtcctccat tttgagctgc ctgcagcagg gccgggaagc ggccatcttt
     7981 ccgctcacgc aactggtgcc gaccgggcca gccttgccgc ccagggcggg gcgatacacg
     8041 gcggcgcgag gccaggcacc agagcaggcc ggccagcttg agactacccc cgtccgattc
     8101 tcggtggccg cgctcgcagg ccccgcctcg ccgaacatgt gcgctgggac gcacgggccc
     8161 cgtcgccgcc cgcggcccca aaaaccgaaa taccagtgtg cagatcttgg cccgcattta
     8221 caagactatc ttgccagaaa aaaagcgtcg cagcaggtca tcaaaaattt taaatggcta
     8281 gagacttatc gaaagcagcg agacaggcgc gaaggtgcca ccagattcgc acgcggcggc
     8341 cccagcgccc aggccaggcc tcaactcaag cacgaggcga aggggctcct taagcgcaag
     8401 gcctcgaact ctcccaccca cttccaaccc gaagctcggg atcaagaatc acgtactgca
     8461 gccaggtgga agtaattcaa ggcacgcaag ggccataacc cgtaaagagg ccaggcccgc
     8521 gggaaccaca cacggcactt acctgtgttc tggcggcaaa cccgttgcga aaaagaacgt
     8581 tcacggcgac tactgcactt atatacggtt ctcccccacc ctcgggaaaa aggcggagcc
     8641 agtacacgac atcactttcc cagtttaccc cgcgccacct tctctaggca ccggttcaat
     8701 tgccgacccc tccccccaac ttctcgggga ctgtgggcga tgtgcgctct gcccactgac
     8761 gggcaccgga gcc
//
