LOCUS       Exported                6656 bp ds-DNA     linear   SYN 15-APR-2025
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6656
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          3309..3875
                     /label=HRAS_NM_005343
     misc_feature    1..2169
                     /label=PCR #1 (pF-BiBiTsmC)
                     /note="PCR #1 (pF-BiBiTsmC)"
     misc_feature    1..24
                     /label=BFBRSG15
                     /note="BFBRSG15"
     misc_feature    complement(1..17)
                     /label=BFBRSG16
                     /note="BFBRSG16"
     misc_feature    1..17
                     /label=PCR #2 (pF-4B)
                     /note="PCR #2 (pF-4B)"
     polyA_signal    52..273
                     /label=Sv40 late polyadenylation signal
     misc_feature    complement(297..330)
                     /label=Cre site
                     /note="Cre site"
     promoter        339..405
                     /label=EM7 bacterial promoter
     CDS             419..1213
                     /label=KanR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     misc_feature    1220..1257
                     /label=Untitled
     rep_origin      1368..1404
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     polyA_signal    complement(2086..2133)
                     /label=Synthetic PolyAdenylation Signal
     misc_feature    complement(2135..2169)
                     /label=BFBRSG14
                     /note="BFBRSG14"
     misc_feature    2145..2998
                     /label=PCR #2 (pF-4B)
                     /note="PCR #2 (pF-4B)"
     misc_feature    2145..2179
                     /label=BFBRSG13
                     /note="BFBRSG13"
     misc_feature    complement(2157..2555)
                     /label=BlastR
                     /note="BlastR"
     misc_feature    complement(2580..2998)
                     /label=SV40 promoter
                     /note="SV40 promoter"
     misc_feature    complement(2973..2998)
                     /label=BFBRSG16
                     /note="BFBRSG16"
     misc_feature    2990..2998
                     /label=BFBRSG15
                     /note="BFBRSG15"
     misc_feature    2999..3212
                     /label=PCR #1 (pF-BiBiTsmC)
                     /note="PCR #1 (pF-BiBiTsmC)"
     misc_feature    2999
                     /label=PCR #1 (pF-BiBiTsmC)
                     /note="PCR #1 (pF-BiBiTsmC)"
     misc_feature    complement(3020..3207)
                     /label=SV40\polyA\signal
     misc_feature    complement(3213..3260)
                     /label=MCS 2
     misc_feature    complement(3298..3305)
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    3309..3875
                     /label=HRas
                     /note="HRas"
     misc_feature    complement(3877..3884)
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    complement(3885..3896)
                     /label=linker
                     /note="linker"
     misc_feature    complement(3897..4409)
                     /label=NanoLuc
                     /note="NanoLuc"
     misc_feature    4423..4449
                     /label=B globin leader
                     /note="B globin leader"
     misc_feature    complement(4450..4466)
                     /label=SP6 promoter
                     /note="SP6 promoter"
     misc_feature    complement(4469..4514)
                     /label=Minimal CMV Promoter 2
     misc_feature    4492..4514
                     /label=core
                     /note="core"
     promoter        4525..5114
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          5229..5361
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        5405..5424
                     /label=T7 promoter
     misc_feature    5428..5435
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    5437..5688
                     /label=cRaf RBD aa51-134
                     /note="cRaf RBD aa51-134"
     misc_feature    5695..5739
                     /label=HT7 Linker C terminal
     misc_feature    5740..6630
                     /label=HT7 tag C terminal
     misc_feature    6651..6656
                     /label=SbfI
                     /note="SbfI"
ORIGIN
        1 ggcatgcaag ctgatttggc tgctgccacc gctgagcaag gccgcttcga gcagacatga
       61 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
      121 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
      181 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt
      241 ttttaagcaa gtaaaacctc tacaaatgtg gtaaaatctt aattaagaat tctaatataa
      301 cttcgtatag catacattat acgaagttat ggatccactg ttgacaatta atcatcggca
      361 tagtatatcg gcatagtata atacgacaag gtgaggaact aaacccagga ggcagatcat
      421 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
      481 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
      541 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
      601 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
      661 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
      721 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
      781 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
      841 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
      901 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
      961 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     1021 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     1081 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     1141 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     1201 cgagttcttc tgattcgaaa tgaccgacca agcgacgccc aaccggtatc agctcactca
     1261 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     1321 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     1381 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1441 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1501 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1561 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1621 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     1681 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     1741 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     1801 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     1861 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1921 tgcaagcagc agattacgcg cagaaaaaaa ggatttcaag aagatccttt gatcttttct
     1981 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     2041 tcaaaaagga tcttcaccta gatcctttta tagtccggaa tcgtcacaca aaaaaccaac
     2101 acacagatgt aatgaaaata aagatatttt attgcggcca tcgtgatggc tagagtttag
     2161 ccctcccaca cataaccaga gggcagcaat tcacgaatcc caactgccgt cggctgtcca
     2221 tcactgtcct tcactatggc tttgatccca ggatgcagat cgagaagcac ctgtcggcac
     2281 cgtccgcagg ggctcaagat gcccctgttc tcatttccga tcgcgacgat acaagtcagg
     2341 ttgccagctg ccgcagcagc agcagtgccc agcaccacga gttctgcaca aggtccccca
     2401 gtaaaatgat atacattgac accagtgaag atgcggccgt cgctagagag agctgcgctg
     2461 gcgacgctgt agtcttcaga gatggggatg ctgttgattg tagccgttgc tctttcaatg
     2521 agggtggatt cttcttgaga caaaggcttg gccatggtgg cgctagtgtc agaagaatcg
     2581 agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg gaatagctca
     2641 gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga
     2701 gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg
     2761 gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
     2821 tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg
     2881 gagcctgggg actttccaca ccctaactga cacacattcc acagctggtt ctttccgcct
     2941 cagaaggtac ctaaccaagt tcctctttca gaggttattt caggccatgg tgctgcgcaa
     3001 gacgcgttaa ttaagacgtc ggcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     3061 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     3121 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
     3181 ctacaaatgt ggtatggctg attatgatcc gcggccgcaa tacgtcgacg ttatcagctg
     3241 acttcgtacg agagcctagg tgtttctaga ggatccccgg gtaccgagcc cgaattcgtt
     3301 taaactcagg agagcacaca cttgcagctc atgcagccgg ggccactctc atcaggaggg
     3361 ttcagcttcc gcagcttgtg ctgccggatc tcacgcacca acgtgtagaa ggcatcctcc
     3421 actccctgcc gggtcttggc cgaggtctcg atgtagggga tgccgtagct tcgggcgagg
     3481 tcctgagcct gccgagattc cacagtgcgt gcagccaggt cacacttgtt ccccaccagc
     3541 accatgggca cgtcatccga gtccttcacc cgtttgatct gctccctgta ctggtggatg
     3601 tcctcaaaag acttggtgtt gttgatggca aacacacaca ggaagccctc cccggtgcgc
     3661 atgtactggt cccgcatggc gctgtactcc tcctggccgg cggtatccag gatgtccaac
     3721 aggcacgtct ccccatcaat gaccacctgc ttccggtagg aatcctctat agtggggtcg
     3781 tattcgtcca caaaatggtt ctggatcagc tggatggtca gcgcactctt gcccacaccg
     3841 ccggcgccca ccaccaccag cttatattcc gtcatggcga tcgcgccgct cgagcccgcc
     3901 agaatgcgtt cgcacagccg ccagccggtc actccgttga tggttactcg gaacagcagg
     3961 gagccgtcgg ggttgatcag gcgctcgtcg ataattttgt tgccgttcca cagggtccct
     4021 gttacagtga tctttttgcc gtcgaacacg gcgatgcctt catacggccg tccgaaatag
     4081 tcgatcatgt tcggcgtaac cccgtcgatt accagtgtgc catagtgcag gatcacctta
     4141 aagtgatgat catccacagg gtacaccacc ttaaaaattt tttcgatctg gcccatttgg
     4201 tcgccgctca gaccttcata cgggatgatg acatggatgt cgatcttcag cccattttca
     4261 ccgctcagga caatcctttg gatcggagtt acggacaccc cgagattctg aaacaaactg
     4321 gacacacctc cctgttcaag gacttggtcc aggttgtagc cggctgtctg tcgccagtcc
     4381 ccaacgaaat cttcgagtgt gaagaccatg gtgagcgcta gctgcaaaaa gaacaagtag
     4441 cttgtattct atagtgtcac ctaaatgggc gatctgacgg ttcactaaac cagctctgct
     4501 tatataggcc tccctggcgc gccactagtt attaatagta atcaattacg gggtcattag
     4561 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     4621 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     4681 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     4741 cagtacatca agtgtatcat atgccaagtc cgccccctat tgacgtcaat gacggtaaat
     4801 ggcccgcctg gcattatgcc cagtacatga ccttacggga ctttcctact tggcagtaca
     4861 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac accaatgggc
     4921 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     4981 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaataac cccgccccgt
     5041 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag
     5101 tgaaccgtca gatcactaga agctttattg cggtagttta tcacagttaa attgctaacg
     5161 cagtcagtgc ttctgacaca acagtctcga acttaagctg cagaagttgg tcgtgaggca
     5221 ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct
     5281 tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc
     5341 actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct taaggctaga
     5401 gtattaatac gactcactat agggctagcg atcgccatgc cttctaagac aagcaacact
     5461 atccgtgttt tcttgccgaa caagcaaaga acagtggtca atgtgcgaaa tggaatgagc
     5521 ttgcatgact gccttatgaa agcactcaag gtgaggggcc tgcaaccaga gtgctgtgca
     5581 gtgttcagac ttctccacga acacaaaggt aaaaaagcac gcttagattg gaatactgat
     5641 gctgcgtctt tgattggaga agaacttcaa gtagatttcc tggatcatgt ttctctcgag
     5701 ccaaccactg aggatctgta ctttcagagc gataacgatg gatccgaaat cggtactggc
     5761 tttccattcg acccccatta tgtggaagtc ctgggcgagc gcatgcacta cgtcgatgtt
     5821 ggtccgcgcg atggcacccc tgtgctgttc ctgcacggta acccgacctc ctcctacgtg
     5881 tggcgcaaca tcatcccgca tgttgcaccg acccatcgct gcattgctcc agacctgatc
     5941 ggtatgggca aatccgacaa accagacctg ggttatttct tcgacgacca cgtccgcttc
     6001 atggatgcct tcatcgaagc cctgggtctg gaagaggtcg tcctggtcat tcacgactgg
     6061 ggctccgctc tgggtttcca ctgggccaag cgcaatccag agcgcgtcaa aggtattgca
     6121 tttatggagt tcatccgccc tatcccgacc tgggacgaat ggccagaatt tgcccgcgag
     6181 accttccagg ccttccgcac caccgacgtc ggccgcaagc tgatcatcga tcagaacgtt
     6241 tttatcgagg gtacgctgcc gatgggtgtc gtccgcccgc tgactgaagt cgagatggac
     6301 cattaccgcg agccgttcct gaatcctgtt gaccgcgagc cactgtggcg cttcccaaac
     6361 gagctgccaa tcgccggtga gccagcgaac atcgtcgcgc tggtcgaaga atacatggac
     6421 tggctgcacc agtcccctgt cccgaagctg ctgttctggg gcaccccagg cgttctgatc
     6481 ccaccggccg aagccgctcg cctggccaaa agcctgccta actgcaaggc tgtggacatc
     6541 ggcccgggtc tgaatctgct gcaagaagac aacccggacc tgatcggcag cgagatcgcg
     6601 cgctggctgt ctactctgga gatttccggt taatagaatt ctagagtcga cctgca
//
