LOCUS       Exported                6687 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF-Nc5K3      5128 bp    DNA   circular            
            3/20/2012

            DEFINITION  Current Name: pF-Nc5K3
FEATURES             Location/Qualifiers
     source          1..6687
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 promoter
     misc_feature    1056..1063
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1065..3008
                     /label=cRaf
                     /note="cRaf"
     misc_feature    3015..3029
                     /label=linker
                     /note="linker"
     misc_feature    3030..3539
                     /label=NanoLuc
                     /note="NanoLuc"
     polyA_signal    3676..3897
                     /label=Sv40 late polyadenylation signal
     promoter        3996..4414
                     /label=SV40 promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     promoter        4422..4488
                     /label=EM7 bacterial promoter
     CDS             4502..5296
                     /label=NeoR / KanR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    5360..5408
                     /label=Synthetic PolyAdenylation Signal
     rep_origin      5644..5680
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatggag cacatacagg
     1081 gagcttggaa gacgatcagc aatggttttg gattcaaaga tgccgtgttt gatggctcca
     1141 gctgcatctc tcctacaata gttcagcagt ttggctatca gcgccgggca tcagatgatg
     1201 gcaaactcac agatccttct aagacaagca acactatccg tgttttcttg ccgaacaagc
     1261 aaagaacagt ggtcaatgtg cgaaatggaa tgagcttgca tgactgcctt atgaaagcac
     1321 tcaaggtgag gggcctgcaa ccagagtgct gtgcagtgtt cagacttctc cacgaacaca
     1381 aaggtaaaaa agcacgctta gattggaata ctgatgctgc gtctttgatt ggagaagaac
     1441 ttcaagtaga tttcctggat catgttcccc tcacaacaca caactttgct cggaagacgt
     1501 tcctgaagct tgccttctgt gacatctgtc agaaattcct gctcaatgga tttcgatgtc
     1561 agacttgtgg ctacaaattt catgagcact gtagcaccaa agtacctact atgtgtgtgg
     1621 actggagtaa catcagacaa ctcttattgt ttccaaattc cactattggt gatagtggag
     1681 tcccagcact accttctttg actatgcgtc gtatgcgaga gtctgtttcc aggatgcctg
     1741 ttagttctca gcacagatat tctacacctc acgccttcac ctttaacacc tccagtccct
     1801 catctgaagg ttccctctcc cagaggcaga ggtcgacatc cacacctaat gtccacatgg
     1861 tcagcaccac cctgcctgtg gacagcagga tgattgagga tgcaattcga agtcacagcg
     1921 aatcagcctc accttcagcc ctgtccagta gccccaacaa tctgagccca acaggctggt
     1981 cacagccgaa aacccccgtg ccagcacaaa gagagcgggc accagtatct gggacccagg
     2041 agaaaaacaa aattaggcct cgtggacaga gagattcaag ctattattgg gaaatagaag
     2101 ccagtgaagt gatgctgtcc actcggattg ggtcaggctc ttttggaact gtttataagg
     2161 gtaaatggca cggagatgtt gcagtaaaga tcctaaaggt tgtcgaccca accccagagc
     2221 aattccaggc cttcaggaat gaggtggctg ttctgcgcaa aacacggcat gtgaacattc
     2281 tgcttttcat ggggtacatg acaaaggaca acctggcaat tgtgacccag tggtgcgagg
     2341 gcagcagcct ctacaaacac ctgcatgtcc aggagaccaa gtttcagatg ttccagctaa
     2401 ttgacattgc ccggcagacg gctcagggaa tggactattt gcatgcaaag aacatcatcc
     2461 atagagacat gaaatccaac aatatatttc tccatgaagg cttaacagtg aaaattggag
     2521 attttggttt ggcaacagta aagtcacgct ggagtggttc tcagcaggtt gaacaaccta
     2581 ctggctctgt cctctggatg gccccagagg tgatccgaat gcaggataac aacccattca
     2641 gtttccagtc ggatgtctac tcctatggca tcgtattgta tgaactgatg acgggggagc
     2701 ttccttattc tcacatcaac aaccgagatc agatcatctt catggtgggc cgaggatatg
     2761 cctccccaga tcttagtaag ctatataaga actgccccaa agcaatgaag aggctggtag
     2821 ctgactgtgt gaagaaagta aaggaagaga ggcctctttt tccccagatc ctgtcttcca
     2881 ttgagctgct ccaacactct ctaccgaaga tcaaccggag cgcttccgag ccatccttgc
     2941 atcgggcagc ccacactgag gatatcaatg cttgcacgct gaccacgtcc ccgaggctgc
     3001 ctgtcttcgt ttctctcggc tcgagcggcg tcttcacact cgaagatttc gttggggact
     3061 ggcgacagac agccggctac aacctggacc aagtccttga acagggaggt gtgtccagtt
     3121 tgtttcagaa tctcggggtg tccgtaactc cgatccaaag gattgtcctg agcggtgaaa
     3181 atgggctgaa gatcgacatc catgtcatca tcccgtatga aggtctgagc ggcgaccaaa
     3241 tgggccagat cgaaaaaatt tttaaggtgg tgtaccctgt ggatgatcat cactttaagg
     3301 tgatcctgca ctatggcaca ctggtaatcg acggggttac gccgaacatg atcgactatt
     3361 tcggacggcc gtatgaaggc atcgccgtgt tcgacggcaa aaagatcact gtaacaggga
     3421 ccctgtggaa cggcaacaaa attatcgacg agcgcctgat caaccccgac ggctccctgc
     3481 tgttccgagt aaccatcaac ggagtgaccg gctggcggct gtgcgaacgc attctggcgt
     3541 aaggccgcga ctctagagtc gacctgcagg catgcaagct gatccggctg ctaacaaagc
     3601 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg
     3661 ggcggccgct tcgagcagac atgataagat acattgatga gtttggacaa accacaacta
     3721 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     3781 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     3841 ttcaggggga gatgtgggag gtttttttaa gcaagtaaaa cctctacaaa tgtggtaaaa
     3901 tcgaatttta acaaaatatt aacgcttaca atttcctgat gcggtatttt ctccttacgc
     3961 atctgtgcgg tatttcacac cgcatacgcg gatctgcgca gcaccatggc ctgaaataac
     4021 ctctgaaaga ggaacttggt taggtacctt ctgaggcgga aagaaccagc tgtggaatgt
     4081 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     4141 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     4201 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     4261 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     4321 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     4381 cttttttgga ggcctaggct tttgcaaaaa gcttaattaa ctgttgacaa ttaatcatcg
     4441 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccca ggaggcagat
     4501 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     4561 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     4621 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     4681 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     4741 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     4801 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     4861 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     4921 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     4981 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     5041 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     5101 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     5161 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     5221 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     5281 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     5341 ctgccatcac gatggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt
     5401 tttgtgtgaa tcgatagcga taaggatcct ctttgcgctt gcgttttccc ttgtccagat
     5461 agcccagtag ctgacattca tccggggtca gcaccgtttc tgcggactgg ctttctaccc
     5521 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     5581 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     5641 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     5701 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     5761 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     5821 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     5881 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     5941 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     6001 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     6061 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     6121 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     6181 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ttcaagaaga
     6241 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     6301 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttatagt ccggaaatac
     6361 aggaacgcac gctggatggc ccttcgctgg gatggtgaaa ccatgaaaaa tggcagcttc
     6421 agtggattaa gtgggggtaa tgtggcctgt accctctggt tgcataggta ttcatacggt
     6481 taaaatttat caggcgcgat tgcggcagtt tttcgggtgg tttgttgcca tttttacctg
     6541 tctgctgccg tgatcgcgct gaacgcgttt tagcggtgcg tacaattaag ggattatggt
     6601 aaatccactt actgtctgcc ctcgtagcca tcgagataaa ccgcagtact ccggccacga
     6661 tgcgtccggc gtagaggatc gagatct
//
