LOCUS       Exported                6139 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF4A(CMV)      4123 bp    DNA   circular            
            1/3/2014

            DEFINITION  Current Name: pF4A (CMV)
FEATURES             Location/Qualifiers
     source          1..6139
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          301..870
                     /label=NRas_WT_NM_002524
     misc_feature    complement(15..202)
                     /label=SV40\polyA\signal
     misc_feature    complement(206..255)
                     /label=MCS 2
     misc_feature    complement(293..300)
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    301..870
                     /label=NRas WT
                     /note="NRas WT"
     misc_feature    complement(872..879)
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    complement(880..891)
                     /label=linker
                     /note="linker"
     misc_feature    complement(892..1404)
                     /label=NanoLuc
                     /note="NanoLuc"
     misc_feature    1418..1444
                     /label=B globin leader
                     /note="B globin leader"
     misc_feature    complement(1445..1461)
                     /label=SP6 promoter
                     /note="SP6 promoter"
     misc_feature    complement(1464..1509)
                     /label=Minimal CMV Promoter 2
     misc_feature    1487..1509
                     /label=core
                     /note="core"
     promoter        1519..2073
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     promoter        2091..2157
                     /label=EM7 bacterial promoter
     CDS             2171..2830
                     /label=Chloramphenicol resistance
     rep_origin      2999..3035
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     polyA_signal    complement(3717..3764)
                     /label=Synthetic PolyAdenylation Signal
     CDS             complement(3788..4582)
                     /label=NeoR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     misc_recomb     complement(4596..4890)
                     /label=R4 aatB
                     /note="R4 aatB"
     promoter        complement(4961..6139)
                     /label=EF1a
                     /note="EF1a"
ORIGIN
        1 aagacgcgtg acgtcggcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
       61 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
      121 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
      181 aatgtggtat ggctgattat gatccgcggc cgcaatacgt cgacgttatc agctgacttc
      241 gtacgagagc ctaggtgttt ctagaggatc cccgggtacc gagcccgaat tcgtttaaac
      301 ttacatcacc acacatggca atcccataca accctgagtc ccatcatcac tgctgttgag
      361 ttttttcatt cggtactggc gtatttctct taccagtgtg taaaaagcat cttcaacacc
      421 ctgtctggtc ttggctgagg tttcaatgaa tggaatcccg taactcttgg ccagttcgtg
      481 ggcttgtttt gtatcaactg tccttgttgg caaatcacac ttgtttccca ctagcaccat
      541 aggtacatca tccgagtctt ttactcgctt aatctgctcc ctgtagaggt taatatccgc
      601 aaatgacttg ctattattga tggcaaatac acagaggaag ccttcgcctg tcctcatgta
      661 ttggtctctc atggcactgt actcttcttg tccagctgta tccagtatgt ccaacaaaca
      721 ggtttcacca tctataacca cttgttttct gtaagaatcc tctatggtgg gatcatattc
      781 atctacaaag tggttctgga ttagctggat tgtcagtgcg cttttcccaa caccacctgc
      841 tccaaccacc accagtttgt actcagtcat ggcgatcgcg ccgctcgagc ccgccagaat
      901 gcgttcgcac agccgccagc cggtcactcc gttgatggtt actcggaaca gcagggagcc
      961 gtcggggttg atcaggcgct cgtcgataat tttgttgccg ttccacaggg tccctgttac
     1021 agtgatcttt ttgccgtcga acacggcgat gccttcatac ggccgtccga aatagtcgat
     1081 catgttcggc gtaaccccgt cgattaccag tgtgccatag tgcaggatca ccttaaagtg
     1141 atgatcatcc acagggtaca ccaccttaaa aattttttcg atctggccca tttggtcgcc
     1201 gctcagacct tcatacggga tgatgacatg gatgtcgatc ttcagcccat tttcaccgct
     1261 caggacaatc ctttggatcg gagttacgga caccccgaga ttctgaaaca aactggacac
     1321 acctccctgt tcaaggactt ggtccaggtt gtagccggct gtctgtcgcc agtccccaac
     1381 gaaatcttcg agtgtgaaga ccatggtgag cgctagctgc aaaaagaaca agtagcttgt
     1441 attctatagt gtcacctaaa tgggcgatct gacggttcac taaaccagct ctgcttatat
     1501 aggcctccct ggcgcgccac tagttattaa tagtaatcaa ttacggggtc attagttcat
     1561 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     1621 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     1681 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     1741 catcaagtgt atcatatgcc aagtccgccc cctattgacg tcaatgacgg taaatggccc
     1801 gcctggcatt atgcccagta catgacctta cgggactttc ctacttggca gtacatctac
     1861 gtattagtca tcgctattac catggtgatg cggttttggc agtacaccaa tgggcgtgga
     1921 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     1981 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta ataaccccgc cccgttgacg
     2041 caaatgggcg gtaggcgtgt acggtgggag gtcaaatcga attctaatac tgttgacaat
     2101 taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa ctaaacccag
     2161 gaggcagatc atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
     2221 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
     2281 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
     2341 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat
     2401 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
     2461 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
     2521 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
     2581 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga
     2641 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcactatgg gcaaatatta
     2701 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtctgtga
     2761 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg
     2821 cggggcgtaa gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caaccggtat
     2881 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     2941 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     3001 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     3061 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     3121 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     3181 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     3241 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     3301 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     3361 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     3421 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     3481 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     3541 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatttcaa gaagatcctt
     3601 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     3661 tcatgagatt atcaaaaagg atcttcacct agatcctttt atagtccgga atcgtcacac
     3721 aaaaaaccaa cacacagatg taatgaaaat aaagatattt tattgcggcc atcgtgatgg
     3781 ctagagttca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag
     3841 cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa
     3901 tatcacgggt agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt
     3961 cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccgt
     4021 gggtcacgac gagatcctcg ccgtcgggca tgcgcgcctt gagcctggcg aacagttcgg
     4081 ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca
     4141 tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg
     4201 gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag
     4261 caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc
     4321 ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg
     4381 atagccgcgc tgcctcgtcc tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa
     4441 aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg
     4501 tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt
     4561 gcaatccatc ttgttcaatc atggtggcat cgattcccgg tcaacatcca gtacacctcc
     4621 ggcaccaccg gcttccccaa gggcgccacg ctctcccacc acaacatcct caacaacggc
     4681 tactgggtgg gccgcacggt cggctacacg gagcaggacc gggtgtgtct gccggtgccc
     4741 ttctaccact gcttcggcat ggtcatgggc aacttgggcg ccacctccca cgccgcctgc
     4801 atcgtcatcc cggccccgtc cttcgagccg gcggcgacgc tggaggcggt gcagcgggag
     4861 aggtgcacgt ccctgtacgg cgtccccacg aagccgaatt ccaccacact ggactagatg
     4921 catgctcgac agtgtgatgg atatctgcag aattcggctt tcacgacacc tgaaatggaa
     4981 gaaaaaaact ttgaaccact gtctgaggct tgagaatgaa ccaagatcca aactcaaaaa
     5041 gggcaaattc caaggagaat tacatcaagt gccaagctgg cctaacttca gtctccaccc
     5101 actcagtgtg gggaaactcc atcgcataaa acccctcccc ccaacctaaa gacgacgtac
     5161 tccaaaagct cgagaactaa tcgaggtgcc tggacggcgc ccggtactcc gtggagtcac
     5221 atgaagcgac ggctgaggac ggaaaggccc ttttcctttg tgtgggtgac tcacccgccc
     5281 gctctcccga gcgccgcgtc ctccattttg agctgcctgc agcagggccg ggaagcggcc
     5341 atctttccgc tcacgcaact ggtgccgacc gggccagcct tgccgcccag ggcggggcga
     5401 tacacggcgg cgcgaggcca ggcaccagag caggccggcc agcttgagac tacccccgtc
     5461 cgattctcgg tggccgcgct cgcaggcccc gcctcgccga acatgtgcgc tgggacgcac
     5521 gggccccgtc gccgcccgcg gccccaaaaa ccgaaatacc agtgtgcaga tcttggcccg
     5581 catttacaag actatcttgc cagaaaaaaa gcgtcgcagc aggtcatcaa aaattttaaa
     5641 tggctagaga cttatcgaaa gcagcgagac aggcgcgaag gtgccaccag attcgcacgc
     5701 ggcggcccca gcgcccaggc caggcctcaa ctcaagcacg aggcgaaggg gctccttaag
     5761 cgcaaggcct cgaactctcc cacccacttc caacccgaag ctcgggatca agaatcacgt
     5821 actgcagcca ggtggaagta attcaaggca cgcaagggcc ataacccgta aagaggccag
     5881 gcccgcggga accacacacg gcacttacct gtgttctggc ggcaaacccg ttgcgaaaaa
     5941 gaacgttcac ggcgactact gcacttatat acggttctcc cccaccctcg ggaaaaaggc
     6001 ggagccagta cacgacatca ctttcccagt ttaccccgcg ccaccttctc taggcaccgg
     6061 ttcaattgcc gacccctccc cccaacttct cggggactgt gggcgatgtg cgctctgccc
     6121 actgacgggc accggagcc
//
