LOCUS       Exported                7871 bp ds-DNA     circular SYN 22-APR-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Apr 22, 2019 from SnapGene Viewer 4.3.6
            https://www.snapgene.com
COMMENT     LOCUS pGloSensor(TM)-30F DEVDG 7871 bp DNA circular 29-JAN-2010
FEATURES             Location/Qualifiers
     source          1..7871
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV Promoter
     intron          857..989
                     /label=chimeric intron
     promoter        1033..1052
                     /label=T7 RNA Polymerase promoter (-17 to +3)
     CDS             1082..2725
                     /label=CP358 UG[DEVD]
     misc_feature    1649..1663
                     /label=DEVDG
     polyA_signal    2882..3103
                     /label=Sv40 late polyadenylation signal
     promoter        3202..3620
                     /label=SV40 early enhancer/promoter region
     CDS             3665..5437
                     /label=hRL-Neo
     polyA_signal    5504..5552
                     /label=Synthetic PolyA
     CDS             5813..6673
                     /label=Amp
     rep_origin      6828..6864
                     /direction=RIGHT
                     /label=ColE1
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag gtttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggaattttaa ctttactaag gagcgatcgc
     1081 catgccggga tcaactggta aaatagtacc attacacgct gttaaagttg tcgatcctac
     1141 aacaggaaaa attttggggc caaatgaacc tggagaattg tattttaaag gcccgatgat
     1201 aatgaagggt tattataata atgaagaagc tactaaagca attattgata atgacggatg
     1261 gttgcgctct ggtgatattg cttattatga caatgatggc catttttata ttgtggacag
     1321 gctgaagtca ctgattaaat ataaaggtta tcaggttgca cctgctgaaa ttgagggaat
     1381 actcttacaa catccgtata ttgttgatgc cggcgttact ggtataccgg atgaagccgc
     1441 gggcgagctt ccagctgcag gtgttgtagt acagactgga aaatatctaa acgaacaaat
     1501 cgtacaagat tatgttgcca gtcaagtttc aatagccaaa tggctacgtg gtggggtgaa
     1561 atttttggat gaaattccca aaggatcaac tggaaaaatt gacagaaaag tgttaagaca
     1621 aatgttagaa aaacacacca atggatccga cgaggtggac ggaagcttga aaaacatcct
     1681 gtatggtccg gaaccgttct acccactgga agatggtacc gctggtgaac agatgtttga
     1741 cgcattatct cgttatgcag ctattccggg ctgcatagca ttgacaaatg ctcatacaaa
     1801 agaaaatgtt ttatatgaag agtttctgaa actgtcgtgt cgtttagcgg aaagttttaa
     1861 aaagtatgga ttaaaacaaa acgacacaat agcggtgtgt agcgaaaata gtctgcaatt
     1921 tttccttcct gtaattgcat cattgtatct tggaataatt gtggcacctg ttaacgataa
     1981 atacattgaa cgtgaattaa tacacagtct tggtattgta aaaccacgca tagttttttg
     2041 ctccaagaat acttttcaaa aagtactgaa tgtaaaatct aaattaaaat ctattgaaac
     2101 tattattata ttagacttaa atgaagactt aggaggttat caatgcctca acaactttat
     2161 ttctcaaaat tccgatagta atctggacgt aaaaaaattt aaaccctatt cttttaatcg
     2221 agacgatcag gttgcgtcga ttatgttttc ttctggtaca actggtctgc cgaagggagt
     2281 catgctaact cacaagaata ttgttgcacg attttctatt gcaaaagatc ctacttttgg
     2341 taacgcaatt aatcccacgt cagcaatttt aacggtaata cctttccacc atggttttgg
     2401 tatgatgacc acattaggat actttacttg tggattccga gttgttctaa tgcacacgtt
     2461 tgaagaaaaa ctatttctac aatcattaca agattataaa gtggaaagta ctttacttgt
     2521 accaacatta atggcatttc ttgcaaaaag tgcattagtt gaaaagtacg atttatcgca
     2581 cttaaaagaa attgcatctg gtggcgcacc tttatcaaaa gaaattgggg agatggtgaa
     2641 aaaacggttt aaattaaact ttgtcaggca agggtatgga ttaacagaaa ccacttcggc
     2701 tgttttaatt acaccgaaag gtgtttaaac gaattcgggc tcggtacccg gggttcctct
     2761 agagtcgacc tgcaggcatg caagctgatc cggctgctaa caaagcccga aaggaagctg
     2821 agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcg gccgcttcga
     2881 gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     2941 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     3001 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg
     3061 tgggaggttt ttttaagcaa gtaaaacctc tacaaatgtg gtaaaatcga attttaacaa
     3121 aatattaacg cttacaattt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     3181 tcacaccgca tacgcggatc tgcgcagcac catggcctga aataacctct gaaagaggaa
     3241 cttggttagg taccttctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt
     3301 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     3361 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     3421 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     3481 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     3541 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     3601 taggcttttg caaaaaggtt gattcttctg acacaacagt ctcgaaccaa aggctggagc
     3661 caccatggct tccaaggtgt acgaccccga gcaacgcaaa cgcatgatca ctgggcctca
     3721 gtggtgggct cgctgcaagc aaatgaacgt gctggactcc ttcatcaact actatgattc
     3781 cgagaagcac gccgagaacg ccgtgatttt tctgcatggt aacgctgcct ccagctacct
     3841 gtggaggcac gtcgtgcctc acatcgagcc cgtggctaga tgcatcatcc ctgatctgat
     3901 cggaatgggt aagtccggca agagcgggaa tggctcatat cgcctcctgg atcactacaa
     3961 gtacctcacc gcttggttcg agctgctgaa ccttccaaag aaaatcatct ttgtgggcca
     4021 cgactggggg gcttgtctgg cctttcacta ctcctacgag caccaagaca agatcaaggc
     4081 catcgtccat gctgagagtg tcgtggacgt gatcgagtcc tgggacgagt ggcctgacat
     4141 cgaggaggat atcgccctga tcaagagcga agagggcgag aaaatggtgc ttgagaataa
     4201 cttcttcgtc gagaccatgc tcccaagcaa gatcatgcgg aaactggagc ctgaggagtt
     4261 cgctgcctac ctggagccat tcaaggagaa gggcgaggtt agacggccta ccctctcctg
     4321 gcctcgcgag atccctctcg ttaagggagg caagcccgac gtcgtccaga ttgtccgcaa
     4381 ctacaacgcc taccttcggg ccagcgacga tctgcctaag atgttcatcg agtccgaccc
     4441 tgggttcttt tccaacgcta ttgtcgaggg agctaagaag ttccctaaca ccgagttcgt
     4501 gaaggtgaag ggcctccact tcagccagga ggacgctcca gatgaaatgg gtaagtacat
     4561 caagagcttc gtggagcgcg tgctgaagaa cgagcagacc ggtggtggga gcggaggtgg
     4621 cggatcaggt ggcggaggct ccggagggat tgaacaagat ggattgcacg caggttctcc
     4681 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     4741 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     4801 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     4861 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     4921 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     4981 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     5041 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     5101 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     5161 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     5221 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     5281 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     5341 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     5401 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     5461 atgaccgacc aagcgacgcc caacctgcca tcacgatggc cgcaataaaa tatctttatt
     5521 ttcattacat ctgtgtgttg gttttttgtg tgaatcgata gcgataaggt tcctctttgc
     5581 gcttgcgttt tcccttgtcc agatagccca gtagctgaca ttcatccggg gtcagcaccg
     5641 tttctgcgga ctggctttct acgtaatggt ttcttagacg tcaggtggca cttttcgggg
     5701 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     5761 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     5821 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     5881 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     5941 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     6001 ttttccaatg atgagcactt tcaaagttct gctatgtggc gcggtattat cccgtattga
     6061 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     6121 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     6181 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacta tcggaggacc
     6241 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     6301 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     6361 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     6421 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     6481 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     6541 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     6601 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     6661 taagcattgg taattcgaaa tgaccgacca agcgacgccc aaccggtatc agctcactca
     6721 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     6781 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     6841 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     6901 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     6961 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7021 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7081 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7141 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7201 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7261 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7321 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7381 tgcaagcagc agattacgcg cagaaaaaaa ggatttcaag aagatccttt gatcttttct
     7441 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7501 tcaaaaagga tcttcaccta gatcctttta tagtccggaa atacaggaac gcacgctgga
     7561 tggcccttcg ctgggatggt gaaaccatga aaaatggcag cttcagtgga ttaagtgggg
     7621 gtaatgtggc ctgtaccctc tggttgcata ggtattcata cggttaaaat ttatcaggcg
     7681 cgattgcggc agtttttcgg gtggtttgtt gccattttta cctgtctgct gccgtgatcg
     7741 cgctgaacgc gttttagcgg tgcgtacaat taagggatta tggtaaatcc acttactgtc
     7801 tgccctcgta gccatcgaga taaaccgcag tactccggcc acgatgcgtc cggcgtagag
     7861 gatcgagatc t
//
