LOCUS       Exported                6633 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF-5A      5591 bp    DNA   circular            
            7/17/2012

            DEFINITION  Current Name: pF-5A
FEATURES             Location/Qualifiers
     source          1..6633
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 RNA Polymerase promoter  (-17 to +3)
     CDS             1065..1100
                     /codon_start=1
                     /label=HiBiT
                     /note="HiBiT"
                     /translation="MVSGWRLFKKIS"
     CDS             1101..1124
                     /codon_start=1
                     /label=8GS linker
                     /note="8GS linker"
                     /translation="GSSGGSSG"
     misc_feature    1125..1132
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1134..2465
                     /label=VPS4B
                     /note="VPS4B"
     misc_feature    2466..2473
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     polyA_signal    2625..2846
                     /label=Sv40 late polyadenylation signal
     promoter        2945..3363
                     /label=SV40 promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     CDS             3408..4202
                     /label=NeoR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    4266..4314
                     /label=Synthetic PolyAdenylation Signal
     CDS             4575..5435
                     /label=AmpR
                     /note="Removed RE site from standard beta-lactamase gene"
     rep_origin      5590..5626
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgct caccatggtg agcggctggc
     1081 ggctgttcaa gaagattagc gggagctccg gtggctcgag cggtgcgatc gccatgtcat
     1141 ccacttcgcc caacctccag aaagcgatag atctggctag caaagcagcg caagaagaca
     1201 aggctgggaa ctacgaagaa gcccttcagc tctatcagca tgctgtgcag tattttcttc
     1261 atgtcgttaa atatgaagca cagggtgata aagccaagca aagtatcagg gcaaagtgta
     1321 cagaatatct tgatagagca gaaaaactaa aggagtacct gaaaaataaa gagaaaaaag
     1381 cacagaagcc agtgaaagaa ggacagccga gtccagcaga tgagaagggg aatgacagtg
     1441 atggggaagg agaatctgat gatcctgaaa aaaagaaact acagaatcaa cttcaaggtg
     1501 ccattgttat agaacgacca aatgtgaaat ggagtgacgt tgctggactt gaaggagcca
     1561 aagaagcact gaaagaggct gtgatactgc ctattaaatt tcctcatctt tttacaggca
     1621 agagaacacc ttggagggga atcctattat ttgggccgcc tggaacagga aagtcctact
     1681 tagccaaagc tgtagcaaca gaagccaaca actcaacatt tttttcaata tcttcctctg
     1741 atcttgtttc taagtggcta ggtgaaagtg aaaaactggt taagaattta ttccaacttg
     1801 ccagagagaa caagccctcc attatcttca ttgatgaaat tgattctctc tgtggttcaa
     1861 gaagtgaaaa tgaaagtgaa gccgcacgta gaattaagac ggagttccta gtgcaaatgc
     1921 aaggggttgg tgtagacaat gatggaattt tggttctggg agctacaaat ataccctggg
     1981 ttctggattc tgccattagg cgaagatttg agaaacgaat ttatattccc ttgccggaac
     2041 cccatgcccg agcagcaatg tttaaactgc acctagggac cactcagaac agtctcacgg
     2101 aagcagactt tcgggaactt gggaggaaaa cagatggtta ttcaggggca gatataagta
     2161 tcattgtacg tgatgccctt atgcagcctg ttaggaaagt acagtcagct actcatttta
     2221 aaaaggttcg cggaccttcc cgagctgatc ctaaccatct tgtagatgat ctgctaacac
     2281 cttgctctcc aggtgaccct ggtgccattg aaatgacatg gatggatgtc cctggagata
     2341 aacttttgga gccagttgtt tccatgtcgg atatgttgcg gtcactatct aacacaaaac
     2401 ctacagtcaa tgaacatgac ttgttgaaat taaagaagtt tacagaagat tttggtcaag
     2461 aaggcgttta aacgaattcg ggctcggtac ccggggatcc tctagagtcg acctgcaggc
     2521 atgcaagctg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc
     2581 gctgagcaat aactagcata accccttggg gcggccgctt cgagcagaca tgataagata
     2641 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     2701 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     2761 caacaattgc attcatttta tgtttcaggt tcagggggag atgtgggagg tttttttaag
     2821 caagtaaaac ctctacaaat gtggtaaaat cgaattttaa caaaatatta acgcttacaa
     2881 tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgcgg
     2941 atctgcgcag caccatggcc tgaaataacc tctgaaagag gaacttggtt aggtaccttc
     3001 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     3061 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     3121 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     3181 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     3241 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     3301 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     3361 cttgattctt ctgacacaac agtctcgaac ttaaggctag agccaccatg attgaacaag
     3421 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     3481 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     3541 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     3601 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     3661 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     3721 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     3781 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     3841 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     3901 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     3961 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     4021 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     4081 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     4141 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     4201 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgatg
     4261 gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga
     4321 tagcgataag gatcctcttt gcgcttgcgt tttcccttgt ccagatagcc cagtagctga
     4381 cattcatccg gggtcagcac cgtttctgcg gactggcttt ctacgtaatg gtttcttaga
     4441 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     4501 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     4561 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     4621 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     4681 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     4741 agagttttcg ccccgaagaa cgttttccaa tgatgagcac tttcaaagtt ctgctatgtg
     4801 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     4861 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     4921 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     4981 ttctgacaac tatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     5041 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     5101 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     5161 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     5221 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     5281 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     5341 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     5401 ctgagatagg tgcctcactg attaagcatt ggtaattcga aatgaccgac caagcgacgc
     5461 ccaaccggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     5521 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     5581 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     5641 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     5701 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     5761 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     5821 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     5881 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     5941 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     6001 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     6061 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     6121 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatttca
     6181 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     6241 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt tatagtccgg
     6301 aaatacagga acgcacgctg gatggccctt cgctgggatg gtgaaaccat gaaaaatggc
     6361 agcttcagtg gattaagtgg gggtaatgtg gcctgtaccc tctggttgca taggtattca
     6421 tacggttaaa atttatcagg cgcgattgcg gcagtttttc gggtggtttg ttgccatttt
     6481 tacctgtctg ctgccgtgat cgcgctgaac gcgttttagc ggtgcgtaca attaagggat
     6541 tatggtaaat ccacttactg tctgccctcg tagccatcga gataaaccgc agtactccgg
     6601 ccacgatgcg tccggcgtag aggatcgaga tct
//
