LOCUS       Exported                5226 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       AY864929                5969 bp    DNA     circular SYN 
            31-JAN-2005

            DEFINITION  Luciferase reporter vector pGL4.15[luc2P/Hygro], 
            complete sequence.

            ACCESSION   AY864929

            VERSION     AY864929.1  GI:58201869

            KEYWORDS    .

            SOURCE      Luciferase reporter vector pGL4.15[luc2P/Hygro]

              ORGANISM  Luciferase reporter vector pGL4.15[luc2P/Hygro]

                        other sequences; artificial sequences; vectors.

            REFERENCE   1  (bases 1 to 5969)

              AUTHORS   Paguio,A., Almond,B. and Browning,J.

              TITLE     pGL4 Luciferase Reporter Vectors Technical Manual, 
            TM259

              JOURNAL   Unpublished

            REFERENCE   2  (bases 1 to 5969)

              AUTHORS   Paguio,A., Almond,B. and Browning,J.

              TITLE     Direct Submission

              JOURNAL   Submitted (28-DEC-2004) Scientific Communications, 
            Promega

                        Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, 
            USA
FEATURES             Location/Qualifiers
     source          1..5226
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    20..460
                     /label=IL2 promoter v2
                     /note="IL2 promoter v2"
     CDS             494..1132
                     /codon_start=1
                     /label=NanoLucP
                     /note="NanoLuc; contains hPEST destabilization region"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILANSHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     misc_feature    1172..1393
                     /label=SV40 poly A
                     /note="SV40 poly A"
     misc_feature    1441..1859
                     /label=SV40 promoter
                     /note="SV40 promoter"
     CDS             1884..2921
                     /codon_start=1
                     /label=HygroR
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     polyA_signal    2945..2993
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal sequence"
     rep_origin      3317
                     /direction=RIGHT
                     /label=ColEI-derived plasmid replication origin
                     /note="ColEI-derived plasmid replication origin"
     CDS             complement(4108..4968)
                     /codon_start=1
                     /label=AmpR
                     /note="AmpR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    5073..5226
                     /label=synthetic poly(A)
                     /note="synthetic poly(A)"
ORIGIN
        1 ggcctaactg gccggtacct ttattctttt catctgttta ctcttgctct tgtccaccac
       61 aatatgctat tcacatgttc agtgtagttt tatgacaaag aaaattttct gagttacttt
      121 tgtatcccca cccccttaaa gaaaggagga aaaactgttt catacagaag gcgttaattg
      181 catgaattag agctatcacc taagtgtggg ctaatgtaac aaagagggat ttcacctaca
      241 tccattcagt cagtctttgg gggtttaaag aaattccaaa gagtcatcag aagaggaaaa
      301 atgaaggtaa tgttttttca gacaggtaaa gtctttgaaa atatgtgtaa tatgtaaaac
      361 attttgacac ccccataata tttttccaga attaacagta taaattgcat ctcttgttca
      421 agagttccct atcactctct ttaatcacta ctcacagtaa gcttggcaat ccggtactgt
      481 tggtaaagcc accatggtct tcacactcga agatttcgtt ggggactggc gacagacagc
      541 cggctacaac ctggaccaag tccttgaaca gggaggtgtg tccagtttgt ttcagaatct
      601 cggggtgtcc gtaactccga tccaaaggat tgtcctgagc ggtgaaaatg ggctgaagat
      661 cgacatccat gtcatcatcc cgtatgaagg tctgagcggc gaccaaatgg gccagatcga
      721 aaaaattttt aaggtggtgt accctgtgga tgatcatcac tttaaggtga tcctgcacta
      781 tggcacactg gtaatcgacg gggttacgcc gaacatgatc gactatttcg gacggccgta
      841 tgaaggcatc gccgtgttcg acggcaaaaa gatcactgta acagggaccc tgtggaacgg
      901 caacaaaatt atcgacgagc gcctgatcaa ccccgacggc tccctgctgt tccgagtaac
      961 catcaacgga gtgaccggct ggcggctgtg cgaacgcatt ctggcgaatt ctcacggctt
     1021 ccctcccgag gtggaggagc aggccgccgg caccctgccc atgagctgcg cccaggagag
     1081 cggcatggat agacaccctg ctgcttgcgc cagcgccagg atcaacgtct aaggccgcga
     1141 ctctagagtc ggggcggccg gccgcttcga gcagacatga taagatacat tgatgagttt
     1201 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     1261 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     1321 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
     1381 tacaaatgtg gtaaaatcga taaggatccg tttgcgtatt gggcgctctt ccgctgatct
     1441 gcgcagcacc atggcctgaa ataacctctg aaagaggaac ttggttagct accttctgag
     1501 gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     1561 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     1621 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     1681 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     1741 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     1801 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcg
     1861 attcttctga cactagcgcc accatgaaga agcccgaact caccgctacc agcgttgaaa
     1921 aatttctcat cgagaagttc gacagtgtga gcgacctgat gcagttgtcg gagggcgaag
     1981 agagccgagc cttcagcttc gatgtcggcg gacgcggcta tgtactgcgg gtgaatagct
     2041 gcgctgatgg cttctacaaa gaccgctacg tgtaccgcca cttcgccagc gctgcactac
     2101 ccatccccga agtgttggac atcggcgagt tcagcgagag cctgacatac tgcatcagta
     2161 gacgcgccca aggcgttact ctccaagacc tccccgaaac agagctgcct gctgtgttac
     2221 agcctgtcgc cgaagctatg gatgctattg ccgccgccga cctcagtcaa accagcggct
     2281 tcggcccatt cgggccccaa ggcatcggcc agtacacaac ctggcgggat ttcatttgcg
     2341 ccattgctga tccccatgtc taccactggc agaccgtgat ggacgacacc gtgtccgcca
     2401 gcgtagctca agccctggac gaactgatgc tgtgggccga agactgtccc gaggtgcgcc
     2461 acctcgtcca tgccgacttc ggcagcaaca acgtcctgac cgacaacggc cgcatcaccg
     2521 ccgtaatcga ctggtccgaa gctatgttcg gggacagtca gtacgaggtg gccaacatct
     2581 tcttctggcg gccctggctg gcttgcatgg agcagcagac tcgctacttc gagcgccggc
     2641 atcccgagct ggccggcagc cctcgtctgc gagcctacat gctgcgcatc ggcctggatc
     2701 agctctacca gagcctcgtg gacggcaact tcgacgatgc tgcctgggct caaggccgct
     2761 gcgatgccat cgtccgcagc ggggccggca ccgtcggtcg cacacaaatc gctcgccgga
     2821 gcgcagccgt atggaccgac ggctgcgtcg aggtgctggc cgacagcggc aaccgccggc
     2881 ccagtacacg accgcgcgct aaggaggtag gtcgagttta aactctagaa ccggtcatgg
     2941 ccgcaataaa atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgttcgaac
     3001 tagatgctgt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc
     3061 gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg
     3121 acaggtgccg gcagcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     3181 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     3241 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     3301 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     3361 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     3421 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     3481 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     3541 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     3601 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     3661 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     3721 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     3781 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     3841 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     3901 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     3961 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     4021 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagcg
     4081 gccgcaaatg ctaaaccact gcagtggtta ccagtgcttg atcagtgagg caccgatctc
     4141 agcgatctgc ctatttcgtt cgtccatagt ggcctgactc cccgtcgtgt agatcactac
     4201 gattcgtgag ggcttaccat caggccccag cgcagcaatg atgccgcgag agccgcgttc
     4261 accggccccc gatttgtcag caatgaacca gccagcaggg agggccgagc gaagaagtgg
     4321 tcctgctact ttgtccgcct ccatccagtc tatgagctgc tgtcgtgatg ctagagtaag
     4381 aagttcgcca gtgagtagtt tccgaagagt tgtggccatt gctactggca tcgtggtatc
     4441 acgctcgtcg ttcggtatgg cttcgttcaa ctctggttcc cagcggtcaa gccgggtcac
     4501 atgatcaccc atattatgaa gaaatgcagt cagctcctta gggcctccga tcgttgtcag
     4561 aagtaagttg gccgcggtgt tgtcgctcat ggtaatggca gcactacaca attctcttac
     4621 cgtcatgcca tccgtaagat gcttttccgt gaccggcgag tactcaacca agtcgttttg
     4681 tgagtagtgt atacggcgac caagctgctc ttgcccggcg tctatacggg acaacaccgc
     4741 gccacatagc agtactttga aagtgctcat catcgggaat cgttcttcgg ggcggaaaga
     4801 ctcaaggatc ttgccgctat tgagatccag ttcgatatag cccactcttg cacccagttg
     4861 atcttcagca tcttttactt tcaccagcgt ttcggggtgt gcaaaaacag gcaagcaaaa
     4921 tgccgcaaag aagggaatga gtgcgacacg aaaatgttgg atgctcatac tcgtcctttt
     4981 tcaatattat tgaagcattt atcagggtta ctagtacgtc tctcaaggat aagtaagtaa
     5041 tattaaggta cgggaggtat tggacaggcc gcaataaaat atctttattt tcattacatc
     5101 tgtgtgttgg ttttttgtgt gaatcgatag tactaacata cgctctccat caaaacaaaa
     5161 cgaaacaaaa caaactagca aaataggctg tccccagtgc aagtgcaggt gccagaacat
     5221 ttctct
//
