LOCUS       Exported                5573 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       AY864929                5969 bp    DNA     circular SYN 
            31-JAN-2005

            DEFINITION  Luciferase reporter vector pGL4.15[luc2P/Hygro], 
            complete sequence.

            ACCESSION   AY864929

            VERSION     AY864929.1  GI:58201869

            KEYWORDS    .

            SOURCE      Luciferase reporter vector pGL4.15[luc2P/Hygro]

              ORGANISM  Luciferase reporter vector pGL4.15[luc2P/Hygro]

                        other sequences; artificial sequences; vectors.

            REFERENCE   1  (bases 1 to 5969)

              AUTHORS   Paguio,A., Almond,B. and Browning,J.

              TITLE     pGL4 Luciferase Reporter Vectors Technical Manual, 
            TM259

              JOURNAL   Unpublished

            REFERENCE   2  (bases 1 to 5969)

              AUTHORS   Paguio,A., Almond,B. and Browning,J.

              TITLE     Direct Submission

              JOURNAL   Submitted (28-DEC-2004) Scientific Communications, 
            Promega

                        Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, 
            USA
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    20..807
                     /label=ABCA1 promoter
                     /note="ABCA1 promoter"
     misc_feature    56..65
                     /label=NFkB
                     /note="NFkB"
     misc_feature    176..182
                     /label=TBE1
                     /note="TBE1"
     misc_feature    267..273
                     /label=TBE2
                     /note="TBE2"
     misc_feature    543..550
                     /label=SP1
                     /note="SP1"
     misc_feature    560..565
                     /label=E BOX
                     /note="E BOX"
     misc_feature    610..616
                     /label=SP1
                     /note="SP1"
     misc_feature    638..652
                     /label=LXRE
                     /note="LXRE"
     misc_feature    707..807
                     /label=mRNA
                     /note="mRNA"
     CDS             841..1479
                     /codon_start=1
                     /label=NanoLucP
                     /note="NanoLuc; contains hPEST destabilization region"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILANSHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     misc_feature    1519..1740
                     /label=SV40 poly A
                     /note="SV40 poly A"
     misc_feature    1788..2206
                     /label=SV40 promoter
                     /note="SV40 promoter"
     CDS             2231..3268
                     /codon_start=1
                     /label=HygroR
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     polyA_signal    3292..3340
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal sequence"
     rep_origin      3664
                     /direction=RIGHT
                     /label=ColEI-derived plasmid replication origin
                     /note="ColEI-derived plasmid replication origin"
     CDS             complement(4455..5315)
                     /codon_start=1
                     /label=AmpR
                     /note="AmpR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    5420..5573
                     /label=synthetic poly
                     /note="synthetic poly(A) signal/transcriptional pause site"
ORIGIN
        1 ggcctaactg gccggtacct ctcgggtcct ctgagggacc tggggagctc aggctgggaa
       61 tctccaaggc agtaggtcgc ctatcaaaaa tcaaagtcca ggtttgtggg gggaaaacaa
      121 aagcagccca ttacccagag gactgtccgc cttcccctca ccccagccta ggcctttgaa
      181 aggaaacaaa agacaagaca aaatgattgg cgtcctgagg gagattcagc ctagagctct
      241 ctctccccca atccctccct ccggctgagg aaactaacaa aggaaaaaaa aattgcggaa
      301 agcaggattt agaggaagca aattccactg gtgcccttgg ctgccgggaa cgtggactag
      361 agagtctgcg gcgcagcccc gagcccagcg cttcccgcgc gtcttaggcc ggcgggcccg
      421 ggcgggggaa ggggacgcag accgcggacc ctaagacacc tgctgtaccc tccaccccca
      481 ccccacccac ctccccccaa ctccctagat gtgtcgtggg cggctgaacg tcgcccgttt
      541 aaggggcggg ccccggctcc acgtgctttc tgctgagtga ctgaactaca taaacagagg
      601 ccgggaacgg ggcggggagg agggagagca caggctttga ccgatagtaa cctctgcgct
      661 cggtgcagcc gaatctataa aaggaactag tcccggcaaa aaccccgtaa ttgcgagcga
      721 gagtgagtgg ggccgggacc cgcagagccg agccgaccct tctctcccgg gctgcggcag
      781 ggcagggcgg ggagctccgc gcaccaagct tggcaatccg gtactgttgg taaagccacc
      841 atggtcttca cactcgaaga tttcgttggg gactggcgac agacagccgg ctacaacctg
      901 gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc agaatctcgg ggtgtccgta
      961 actccgatcc aaaggattgt cctgagcggt gaaaatgggc tgaagatcga catccatgtc
     1021 atcatcccgt atgaaggtct gagcggcgac caaatgggcc agatcgaaaa aatttttaag
     1081 gtggtgtacc ctgtggatga tcatcacttt aaggtgatcc tgcactatgg cacactggta
     1141 atcgacgggg ttacgccgaa catgatcgac tatttcggac ggccgtatga aggcatcgcc
     1201 gtgttcgacg gcaaaaagat cactgtaaca gggaccctgt ggaacggcaa caaaattatc
     1261 gacgagcgcc tgatcaaccc cgacggctcc ctgctgttcc gagtaaccat caacggagtg
     1321 accggctggc ggctgtgcga acgcattctg gcgaattctc acggcttccc tcccgaggtg
     1381 gaggagcagg ccgccggcac cctgcccatg agctgcgccc aggagagcgg catggataga
     1441 caccctgctg cttgcgccag cgccaggatc aacgtctaag gccgcgactc tagagtcggg
     1501 gcggccggcc gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa
     1561 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     1621 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     1681 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
     1741 aaatcgataa ggatccgttt gcgtattggg cgctcttccg ctgatctgcg cagcaccatg
     1801 gcctgaaata acctctgaaa gaggaacttg gttagctacc ttctgaggcg gaaagaacca
     1861 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     1921 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     1981 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     2041 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     2101 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     2161 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcgatt cttctgacac
     2221 tagcgccacc atgaagaagc ccgaactcac cgctaccagc gttgaaaaat ttctcatcga
     2281 gaagttcgac agtgtgagcg acctgatgca gttgtcggag ggcgaagaga gccgagcctt
     2341 cagcttcgat gtcggcggac gcggctatgt actgcgggtg aatagctgcg ctgatggctt
     2401 ctacaaagac cgctacgtgt accgccactt cgccagcgct gcactaccca tccccgaagt
     2461 gttggacatc ggcgagttca gcgagagcct gacatactgc atcagtagac gcgcccaagg
     2521 cgttactctc caagacctcc ccgaaacaga gctgcctgct gtgttacagc ctgtcgccga
     2581 agctatggat gctattgccg ccgccgacct cagtcaaacc agcggcttcg gcccattcgg
     2641 gccccaaggc atcggccagt acacaacctg gcgggatttc atttgcgcca ttgctgatcc
     2701 ccatgtctac cactggcaga ccgtgatgga cgacaccgtg tccgccagcg tagctcaagc
     2761 cctggacgaa ctgatgctgt gggccgaaga ctgtcccgag gtgcgccacc tcgtccatgc
     2821 cgacttcggc agcaacaacg tcctgaccga caacggccgc atcaccgccg taatcgactg
     2881 gtccgaagct atgttcgggg acagtcagta cgaggtggcc aacatcttct tctggcggcc
     2941 ctggctggct tgcatggagc agcagactcg ctacttcgag cgccggcatc ccgagctggc
     3001 cggcagccct cgtctgcgag cctacatgct gcgcatcggc ctggatcagc tctaccagag
     3061 cctcgtggac ggcaacttcg acgatgctgc ctgggctcaa ggccgctgcg atgccatcgt
     3121 ccgcagcggg gccggcaccg tcggtcgcac acaaatcgct cgccggagcg cagccgtatg
     3181 gaccgacggc tgcgtcgagg tgctggccga cagcggcaac cgccggccca gtacacgacc
     3241 gcgcgctaag gaggtaggtc gagtttaaac tctagaaccg gtcatggccg caataaaata
     3301 tctttatttt cattacatct gtgtgttggt tttttgtgtg ttcgaactag atgctgtcga
     3361 ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact
     3421 atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca
     3481 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     3541 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     3601 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     3661 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     3721 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     3781 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     3841 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     3901 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     3961 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     4021 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     4081 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     4141 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     4201 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     4261 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     4321 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     4381 ttttaaatca atctaaagta tatatgagta aacttggtct gacagcggcc gcaaatgcta
     4441 aaccactgca gtggttacca gtgcttgatc agtgaggcac cgatctcagc gatctgccta
     4501 tttcgttcgt ccatagtggc ctgactcccc gtcgtgtaga tcactacgat tcgtgagggc
     4561 ttaccatcag gccccagcgc agcaatgatg ccgcgagagc cgcgttcacc ggcccccgat
     4621 ttgtcagcaa tgaaccagcc agcagggagg gccgagcgaa gaagtggtcc tgctactttg
     4681 tccgcctcca tccagtctat gagctgctgt cgtgatgcta gagtaagaag ttcgccagtg
     4741 agtagtttcc gaagagttgt ggccattgct actggcatcg tggtatcacg ctcgtcgttc
     4801 ggtatggctt cgttcaactc tggttcccag cggtcaagcc gggtcacatg atcacccata
     4861 ttatgaagaa atgcagtcag ctccttaggg cctccgatcg ttgtcagaag taagttggcc
     4921 gcggtgttgt cgctcatggt aatggcagca ctacacaatt ctcttaccgt catgccatcc
     4981 gtaagatgct tttccgtgac cggcgagtac tcaaccaagt cgttttgtga gtagtgtata
     5041 cggcgaccaa gctgctcttg cccggcgtct atacgggaca acaccgcgcc acatagcagt
     5101 actttgaaag tgctcatcat cgggaatcgt tcttcggggc ggaaagactc aaggatcttg
     5161 ccgctattga gatccagttc gatatagccc actcttgcac ccagttgatc ttcagcatct
     5221 tttactttca ccagcgtttc ggggtgtgca aaaacaggca agcaaaatgc cgcaaagaag
     5281 ggaatgagtg cgacacgaaa atgttggatg ctcatactcg tcctttttca atattattga
     5341 agcatttatc agggttacta gtacgtctct caaggataag taagtaatat taaggtacgg
     5401 gaggtattgg acaggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt
     5461 tttgtgtgaa tcgatagtac taacatacgc tctccatcaa aacaaaacga aacaaaacaa
     5521 actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc tct
//
