LOCUS       Exported                4891 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       DQ904459                6010 bp    DNA     circular SYN 
            26-SEP-2006

            DEFINITION  Firefly luciferase reporter vector 
            pGL4.27[luc2P/minP/Hygro],

                        complete sequence.

            ACCESSION   DQ904459

            VERSION     DQ904459.1  GI:115342887

            KEYWORDS    .

            SOURCE      Firefly luciferase reporter vector 
            pGL4.27[luc2P/minP/Hygro]

              ORGANISM  Firefly luciferase reporter vector 
            pGL4.27[luc2P/minP/Hygro]

                        other sequences; artificial sequences; vectors.

            REFERENCE   1  (bases 1 to 6010)

              AUTHORS   Swanson,B., Kopish,K. and McNamara,B.

              TITLE     pGL4 Luciferase reporter Vectors Technical Manual, 
            TM259

              JOURNAL   Unpublished

            REFERENCE   2  (bases 1 to 6010)

              AUTHORS   Swanson,B., Kopish,K. and McNamara,B.

              TITLE     Direct Submission

              JOURNAL   Submitted (16-AUG-2006) Scientific Communications, 
            Promega

                        Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, 
            USA
FEATURES             Location/Qualifiers
     source          1..4891
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    19..39
                     /label=STAT6 RE
                     /note="STAT6 RE"
     misc_feature    40..60
                     /label=STAT6 RE
                     /note="STAT6 RE"
     misc_feature    61..81
                     /label=STAT6 RE
                     /note="STAT6 RE"
     misc_feature    96..126
                     /label=minimal promoter
                     /note="minimal promoter"
     misc_feature    159..797
                     /label=NanoLucP
                     /note="NanoLucP"
     misc_feature    837..1058
                     /label=PolyA signal
                     /note="PolyA signal"
     misc_feature    1106..1524
                     /label=SV40 promoter
                     /note="SV40 promoter"
     CDS             1549..2586
                     /codon_start=1
                     /label=hygromycin resistance
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     rep_origin      2982
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="ColE1-derived plasmid replication origin"
     gene            complement(3773..4633)
                     /label=AmpR
     polyA_signal    4738..4891
                     /label=poly(A) pause site
                     /note="poly(A) pause site"
ORIGIN
        1 ggcctaactg gccggtacca gtttccgagg aactgggccc agtttccgag gaactgggcc
       61 cagtttccga ggaactgggc cgaagcttag acactagagg gtatataatg gaagctcgac
      121 ttccagcttg gcaatccggt actgttggta aagccaccat ggtcttcaca ctcgaagatt
      181 tcgttgggga ctggcgacag acagccggct acaacctgga ccaagtcctt gaacagggag
      241 gtgtgtccag tttgtttcag aatctcgggg tgtccgtaac tccgatccaa aggattgtcc
      301 tgagcggtga aaatgggctg aagatcgaca tccatgtcat catcccgtat gaaggtctga
      361 gcggcgacca aatgggccag atcgaaaaaa tttttaaggt ggtgtaccct gtggatgatc
      421 atcactttaa ggtgatcctg cactatggca cactggtaat cgacggggtt acgccgaaca
      481 tgatcgacta tttcggacgg ccgtatgaag gcatcgccgt gttcgacggc aaaaagatca
      541 ctgtaacagg gaccctgtgg aacggcaaca aaattatcga cgagcgcctg atcaaccccg
      601 acggctccct gctgttccga gtaaccatca acggagtgac cggctggcgg ctgtgcgaac
      661 gcattctggc gaattctcac ggctttccgc ctgaggttga agagcaagcc gccggtacat
      721 tgcctatgtc ctgcgcacaa gaaagcggta tggaccggca cccagccgct tgtgcttcag
      781 ctcgcatcaa cgtctaaggc cgcgactcta gagtcggggc ggccggccgc ttcgagcaga
      841 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
      901 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
      961 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     1021 ggttttttaa agcaagtaaa acctctacaa atgtggtaaa atcgataagg atccgtttgc
     1081 gtattgggcg ctcttccgct gatctgcgca gcaccatggc ctgaaataac ctctgaaaga
     1141 ggaacttggt tagctacctt ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta
     1201 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     1261 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     1321 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     1381 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     1441 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     1501 ggcctaggct tttgcaaaaa gctcgattct tctgacacta gcgccaccat gaagaagccc
     1561 gaactcaccg ctaccagcgt tgaaaaattt ctcatcgaga agttcgacag tgtgagcgac
     1621 ctgatgcagt tgtcggaggg cgaagagagc cgagccttca gcttcgatgt cggcggacgc
     1681 ggctatgtac tgcgggtgaa tagctgcgct gatggcttct acaaagaccg ctacgtgtac
     1741 cgccacttcg ccagcgctgc actacccatc cccgaagtgt tggacatcgg cgagttcagc
     1801 gagagcctga catactgcat cagtagacgc gcccaaggcg ttactctcca agacctcccc
     1861 gaaacagagc tgcctgctgt gttacagcct gtcgccgaag ctatggatgc tattgccgcc
     1921 gccgacctca gtcaaaccag cggcttcggc ccattcgggc cccaaggcat cggccagtac
     1981 acaacctggc gggatttcat ttgcgccatt gctgatcccc atgtctacca ctggcagacc
     2041 gtgatggacg acaccgtgtc cgccagcgta gctcaagccc tggacgaact gatgctgtgg
     2101 gccgaagact gtcccgaggt gcgccacctc gtccatgccg acttcggcag caacaacgtc
     2161 ctgaccgaca acggccgcat caccgccgta atcgactggt ccgaagctat gttcggggac
     2221 agtcagtacg aggtggccaa catcttcttc tggcggccct ggctggcttg catggagcag
     2281 cagactcgct acttcgagcg ccggcatccc gagctggccg gcagccctcg tctgcgagcc
     2341 tacatgctgc gcatcggcct ggatcagctc taccagagcc tcgtggacgg caacttcgac
     2401 gatgctgcct gggctcaagg ccgctgcgat gccatcgtcc gcagcggggc cggcaccgtc
     2461 ggtcgcacac aaatcgctcg ccggagcgca gccgtatgga ccgacggctg cgtcgaggtg
     2521 ctggccgaca gcggcaaccg ccggcccagt acacgaccgc gcgctaagga ggtaggtcga
     2581 gtttaaactc tagaaccggt catggccgca ataaaatatc tttattttca ttacatctgt
     2641 gtgttggttt tttgtgtgtt cgaactagat gctgtcgacc gatgcccttg agagccttca
     2701 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg
     2761 tcttctttat catgcaactc gtaggacagg tgccggcagc gctcttccgc ttcctcgctc
     2821 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     2881 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     2941 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     3001 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     3061 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     3121 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     3181 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     3241 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     3301 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     3361 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     3421 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     3481 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     3541 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     3601 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     3661 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     3721 tatgagtaaa cttggtctga cagcggccgc aaatgctaaa ccactgcagt ggttaccagt
     3781 gcttgatcag tgaggcaccg atctcagcga tctgcctatt tcgttcgtcc atagtggcct
     3841 gactccccgt cgtgtagatc actacgattc gtgagggctt accatcaggc cccagcgcag
     3901 caatgatgcc gcgagagccg cgttcaccgg cccccgattt gtcagcaatg aaccagccag
     3961 cagggagggc cgagcgaaga agtggtcctg ctactttgtc cgcctccatc cagtctatga
     4021 gctgctgtcg tgatgctaga gtaagaagtt cgccagtgag tagtttccga agagttgtgg
     4081 ccattgctac tggcatcgtg gtatcacgct cgtcgttcgg tatggcttcg ttcaactctg
     4141 gttcccagcg gtcaagccgg gtcacatgat cacccatatt atgaagaaat gcagtcagct
     4201 ccttagggcc tccgatcgtt gtcagaagta agttggccgc ggtgttgtcg ctcatggtaa
     4261 tggcagcact acacaattct cttaccgtca tgccatccgt aagatgcttt tccgtgaccg
     4321 gcgagtactc aaccaagtcg ttttgtgagt agtgtatacg gcgaccaagc tgctcttgcc
     4381 cggcgtctat acgggacaac accgcgccac atagcagtac tttgaaagtg ctcatcatcg
     4441 ggaatcgttc ttcggggcgg aaagactcaa ggatcttgcc gctattgaga tccagttcga
     4501 tatagcccac tcttgcaccc agttgatctt cagcatcttt tactttcacc agcgtttcgg
     4561 ggtgtgcaaa aacaggcaag caaaatgccg caaagaaggg aatgagtgcg acacgaaaat
     4621 gttggatgct catactcgtc ctttttcaat attattgaag catttatcag ggttactagt
     4681 acgtctctca aggataagta agtaatatta aggtacggga ggtattggac aggccgcaat
     4741 aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagtacta
     4801 acatacgctc tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc
     4861 agtgcaagtg caggtgccag aacatttctc t
//
