LOCUS       Exported                7022 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pFN31K(pF-Nn5K3)-SUZ12      6986 bp    DNA   circular   
                    6/10/2016

            DEFINITION  Current Name: pFN31K (pF-Nn5K3) - SUZ12 Old/Alternate 
            Name(s):CHET9 (insert) JJAZ1 (insert) KIAA0160 (insert) suppressor 
            of zeste 12 homolog (Drosophila) (insert) SUZ12 (insert) pF-Nn5K3 
            (backbone)
FEATURES             Location/Qualifiers
     source          1..7022
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..742
                     /label=CMV immediate-early enhancer/promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     intron          857..989
                     /label=chimeric intron
                     /note="Note that this is the actual sequence spliced out. 
                     The 5' donor site includes a 5' GA and the 3' acceptor site
                     adds a 3'G."
     promoter        1033..1052
                     /label=T7 RNA Polymerase promoter
     CDS             1065..1577
                     /label=N terminal NanoLuc
     misc_feature    1578..1589
                     /label=N Terminal NanoLuc linker
     misc_feature    1590..1597
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    1595..3855
                     /label=EZH2 insert
                     /note="Recieved from Kazusa in August 2010. Entered into 
                     database semi automatically by Ethan in August 2010. 
                     Accession number NM_004456 added 11/4/2010 from BLAST and 
                     length similarity. (note added in Automated Blaster by 
                     11/4/2010 3:33:44 PM"
     misc_feature    1820..1825
                     /label=EcoICR I
     misc_feature    2828..2833
                     /label=EcoICR I
     misc_feature    3852..3859
                     /label=Pme I EcoICRI Chimeric Site
                     /note="Created by Flexi cloning into C terminal fusion 
                     vector. Also occurs natually in about 1/3 of inserts."
     misc_feature    3860..3989
                     /label=Transfer Efficiency Cassette
                     /note="Feature changed from 
                     ""AAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAA
                     GCTGATCCGGCTGCTAACAAAGC"" at Jim Hartnet's request 
                     12/18/2007 to include the complete cassette."
     polyA_signal    4011..4232
                     /label=Sv40 late polyadenylation signal
     promoter        4331..4749
                     /label=SV40 enhancer and early promoter
                     /note="Name changed frm ""SV40 early enhancer/promoter 
                     region"" to ""SV40 enhancer and early promoter"" on 
                     3/21/2011 at Jim Hartnet's request to fit Sci Com's 
                     naming."
     enhancer        complement(4404..4640)
                     /label=SV40 Enhancer
                     /note="Sequence shown is from SV40 genome. Our vectors 
                     contain sequences with one or two point mutations in first 
                     few bases. These apparently do not affect function. The 
                     sequences present in our vectors are shown to the right."
     rep_origin      4647..4712
                     /direction=RIGHT
                     /label=Sv40 Min Ori
     promoter        4757..4823
                     /label=EM7 bacterial promoter
     CDS             4837..5631
                     /label=Neo-Kan Resistance
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     polyA_signal    5695..5743
                     /label=Synthetic PolyAdenylation Signal
     rep_origin      5979..6015
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     misc_feature    6686..6971
                     /label=Cer Region
                     /note="Prevents multimerization"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgct caccatggtc ttcacactcg
     1081 aagatttcgt tggggactgg cgacagacag ccggctacaa cctggaccaa gtccttgaac
     1141 agggaggtgt gtccagtttg tttcagaatc tcggggtgtc cgtaactccg atccaaagga
     1201 ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag
     1261 gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt taaggtggtg taccctgtgg
     1321 atgatcatca ctttaaggtg atcctgcact atggcacact ggtaatcgac ggggttacgc
     1381 cgaacatgat cgactatttc ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa
     1441 agatcactgt aacagggacc ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca
     1501 accccgacgg ctccctgctg ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt
     1561 gcgaacgcat tctggcgggc tcgagcggcg cgatcgccat gggccagact gggaagaaat
     1621 ctgagaaggg accagtttgt tggcggaagc gtgtaaaatc agagtacatg cgactgagac
     1681 agctcaagag gttcagacga gctgatgaag taaagagtat gtttagttcc aatcgtcaga
     1741 aaattttgga aagaacggaa atcttaaacc aagaatggaa acagcgaagg atacagcctg
     1801 tgcacatcct gacttctgtg agctcattgc gcgggactag ggagtgttcg gtgaccagtg
     1861 acttggattt tccaacacaa gtcatcccat taaagactct gaatgcagtt gcttcagtac
     1921 ccataatgta ttcttggtct cccctacagc agaattttat ggtggaagat gaaactgttt
     1981 tacataacat tccttatatg ggagatgaag ttttagatca ggatggtact ttcattgaag
     2041 aactaataaa aaattatgat gggaaagtac acggggatag agaatgtggg tttataaatg
     2101 atgaaatttt tgtggagttg gtgaatgccc ttggtcaata taatgatgat gacgatgatg
     2161 atgatggaga cgatcctgaa gaaagagaag aaaagcagaa agatctggag gatcaccgag
     2221 atgataaaga aagccgccca cctcggaaat ttccttctga taaaattttt gaagccattt
     2281 cctcaatgtt tccagataag ggcacagcag aagaactaaa ggaaaaatat aaagaactca
     2341 ccgaacagca gctcccaggc gcacttcctc ctgaatgtac ccccaacata gatggaccaa
     2401 atgctaaatc tgttcagaga gagcaaagct tacactcctt tcatacgctt ttctgtaggc
     2461 gatgttttaa atatgactgc ttcctacatc gtaagtgcaa ttattctttt catgcaacac
     2521 ccaacactta taagcggaag aacacagaaa cagctctaga caacaaacct tgtggaccac
     2581 agtgttacca gcatttggag ggagcaaagg agtttgctgc tgctctcacc gctgagcgga
     2641 taaagacccc accaaaacgt ccaggaggcc gcagaagagg acggcttccc aataacagta
     2701 gcaggcccag cacccccacc attaatgtgc tggaatcaaa ggatacagac agtgataggg
     2761 aagcagggac tgaaacgggg ggagagaaca atgataaaga agaagaagag aagaaagatg
     2821 aaacttcgag ctcctctgaa gcaaattctc ggtgtcaaac accaataaag atgaagccaa
     2881 atattgaacc tcctgagaat gtggagtgga gtggtgctga agcctcaatg tttagagtcc
     2941 tcattggcac ttactatgac aatttctgtg ccattgctag gttaattggg accaaaacat
     3001 gtagacaggt gtatgagttt agagtcaaag aatctagcat catagctcca gctcccgctg
     3061 aggatgtgga tactcctcca aggaaaaaga agaggaaaca ccggttgtgg gctgcacact
     3121 gcagaaagat acagctgaaa aaggacggct cctctaacca tgtttacaac tatcaaccct
     3181 gtgatcatcc acggcagcct tgtgacagtt cgtgcccttg tgtgatagca caaaattttt
     3241 gtgaaaagtt ttgtcaatgt agttcagagt gtcaaaaccg ctttccggga tgccgctgca
     3301 aagcacagtg caacaccaag cagtgcccgt gctacctggc tgtccgagag tgtgaccctg
     3361 acctctgtct tacttgtgga gccgctgacc attgggacag taaaaatgtg tcctgcaaga
     3421 actgcagtat tcagcggggc tccaaaaagc atctattgct ggcaccatct gacgtggcag
     3481 gctgggggat ttttatcaaa gatcctgtgc agaaaaatga attcatctca gaatactgtg
     3541 gagagattat ttctcaagat gaagctgaca gaagagggaa agtgtatgat aaatacatgt
     3601 gcagctttct gttcaacttg aacaatgatt ttgtggtgga tgcaacccgc aagggtaaca
     3661 aaattcgttt tgcaaatcat tcggtaaatc caaactgcta tgcaaaagtt atgatggtta
     3721 acggtgatca caggataggt atttttgcca agagagccat ccagactggc gaagagctgt
     3781 tttttgatta cagatacagc caggctgatg ccctgaagta tgtcggcatc gaaagagaaa
     3841 tggaaatccc tgtttaaacg aattcgggct cggtacccgg ggatcctcta gagtcgacct
     3901 gcaggcatgc aagctgatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct
     3961 gccaccgctg agcaataact agcataaccc cttggggcgg ccgcttcgag cagacatgat
     4021 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     4081 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     4141 taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt
     4201 tttaagcaag taaaacctct acaaatgtgg taaaatcgaa ttttaacaaa atattaacgc
     4261 ttacaatttc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     4321 acgcggatct gcgcagcacc atggcctgaa ataacctctg aaagaggaac ttggttaggt
     4381 accttctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     4441 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     4501 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4561 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     4621 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     4681 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     4741 aaaaagctta attaactgtt gacaattaat catcggcata gtatatcggc atagtataat
     4801 acgacaaggt gaggaactaa acccaggagg cagatcatga ttgaacaaga tggattgcac
     4861 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     4921 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     4981 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
     5041 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     5101 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     5161 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     5221 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     5281 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     5341 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
     5401 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     5461 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     5521 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     5581 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     5641 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgatgg ccgcaataaa
     5701 atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat agcgataagg
     5761 atcctctttg cgcttgcgtt ttcccttgtc cagatagccc agtagctgac attcatccgg
     5821 ggtcagcacc gtttctgcgg actggctttc tacccggtat cagctcactc aaaggcggta
     5881 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     5941 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     6001 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     6061 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     6121 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     6181 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     6241 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     6301 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     6361 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     6421 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     6481 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     6541 cagattacgc gcagaaaaaa aggatttcaa gaagatcctt tgatcttttc tacggggtct
     6601 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     6661 atcttcacct agatcctttt atagtccgga aatacaggaa cgcacgctgg atggcccttc
     6721 gctgggatgg tgaaaccatg aaaaatggca gcttcagtgg attaagtggg ggtaatgtgg
     6781 cctgtaccct ctggttgcat aggtattcat acggttaaaa tttatcaggc gcgattgcgg
     6841 cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc gcgctgaacg
     6901 cgttttagcg gtgcgtacaa ttaagggatt atggtaaatc cacttactgt ctgccctcgt
     6961 agccatcgag ataaaccgca gtactccggc cacgatgcgt ccggcgtaga ggatcgagat
     7021 ct
//
