LOCUS       Exported                6769 bp ds-DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pF4A(CMV)      4123 bp    DNA   circular            
            1/3/2014

            DEFINITION  Current Name: pF4A (CMV)
FEATURES             Location/Qualifiers
     source          1..6769
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(15..202)
                     /label=SV40\polyA\signal
     misc_feature    complement(206..255)
                     /label=MCS 2
     misc_feature    complement(293..300)
                     /label=PmeI site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    301..1500
                     /label=TAZ
                     /note="TAZ"
     misc_feature    complement(1502..1509)
                     /label=SgfI Site
                     /note="Used in Flexi Vector Cloning"
     misc_feature    complement(1510..1521)
                     /label=linker
                     /note="linker"
     misc_feature    complement(1522..2034)
                     /label=NanoLuc
                     /note="NanoLuc"
     misc_feature    2048..2074
                     /label=B globin leader
                     /note="B globin leader"
     misc_feature    complement(2075..2091)
                     /label=SP6 promoter
                     /note="SP6 promoter"
     misc_feature    complement(2094..2139)
                     /label=Minimal CMV Promoter 2
     misc_feature    2117..2139
                     /label=core
                     /note="core"
     promoter        2149..2703
                     /label=CMV promoter
                     /note="Has EcoICRI site removed. Named changed from ""CMV 
                     Promoter"" to ""CMV immediate-early enhancer/promoter"" 
                     3/21/2011 and J Hartnet's request to fit Sci Com's naming."
     promoter        2721..2787
                     /label=EM7 bacterial promoter
     CDS             2801..3460
                     /label=Chloramphenicol resistance
     rep_origin      3629..3665
                     /direction=RIGHT
                     /label=ColE1-derived plasmid replication origin
                     /note="The actual origin is the first base."
     polyA_signal    complement(4347..4394)
                     /label=Synthetic PolyAdenylation Signal
     CDS             complement(4418..5212)
                     /label=NeoR
                     /note="Name changed frm ""Neomycin phosphotransferase"" to 
                     ""Neomycin phosphotransferase coding region"" on 3/21/2011 
                     at Jim Hartnet's request to fit Sci Com's naming."
     misc_recomb     complement(5226..5520)
                     /label=R4 aatB
                     /note="R4 aatB"
     promoter        complement(5591..6769)
                     /label=EF1a
                     /note="EF1a"
ORIGIN
        1 aagacgcgtg acgtcggcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
       61 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
      121 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
      181 aatgtggtat ggctgattat gatccgcggc cgcaatacgt cgacgttatc agctgacttc
      241 gtacgagagc ctaggtgttt ctagaggatc cccgggtacc gagcccgaat tcgtttaaac
      301 cagccaggtt agaaagggct cacttttgtt cagagcagac tctacatcat tgaagagggg
      361 gatcaggtct tcagattcca aagttcctaa gtcaacgttt gttcctggaa gacagtcaag
      421 gaaatcaggg aaacgggtct gttggggatt gatgttcatg ggtgtttgtc ctgcgttttc
      481 tcctgtatcc atctcatcca cattgctgag gaagtcctcc ggagttgtgg ggacactgta
      541 gcaccctaac cccaggccac tgtcagtgct ctgctccctc gaatgatatg gccctccatt
      601 gaggaaagga tctgagctat tattagtgat ggatctcatg tctggggtca tcgtgggtgg
      661 gttgacagca gcctgaactg gggcaagagt ctcagcttcc atggggagct gtcgacagag
      721 ggcagcttcc tgcctcatga gctcctcttg gcgcattcga atcctttctc tctccatctg
      781 gattctctga agccgcagtt tctgctgctg ctgctgctga gtggtcagcg cattgggcat
      841 actcatgagc cctgcgggtg ggttctgagt ggggtggttc tgctggctca gggtactggg
      901 ggccatctgc tgctggtgtt ggtgattcat cacgagattt ggctgggata ctgccatgga
      961 cctctgaggc actggtgtgg aactgacggc agggtggagg ttcatatgat tcagaggctg
     1021 attcatcgcc ttcctagggt cttgccatgt ggtgattttt tctatgtgat tgaggaagta
     1081 cctctggcca gtggccgtga aggtcatctc ccagcccggg ggcagtggca gctcgtcggt
     1141 cacgtcgtag gactgctggc ggaggtgcgc gtgctgctgc gcggggctac ccgcagcacc
     1201 cgcgccggtg cccagctgca gggacgcggg cgacgagtgc gagcggacat gctgggcacc
     1261 cccagccagt cgaggccccg ggtggccgcc cgacgagtcg gtgctggact ggcgcgagtg
     1321 cgagcccgaa tcaggctcct taaagaaaga ctccggcagg atcttcttcc gccacgagct
     1381 aggcttcgga ttcatgacag agttgaagag ggcttcgagg tctgtgtcta ggtcctgcgt
     1441 gacgtggatc acttgctgcc caggcggcgg gagcggaggg ggcgccgagg ccggattcat
     1501 ggcgatcgcg ccgctcgagc ccgccagaat gcgttcgcac agccgccagc cggtcactcc
     1561 gttgatggtt actcggaaca gcagggagcc gtcggggttg atcaggcgct cgtcgataat
     1621 tttgttgccg ttccacaggg tccctgttac agtgatcttt ttgccgtcga acacggcgat
     1681 gccttcatac ggccgtccga aatagtcgat catgttcggc gtaaccccgt cgattaccag
     1741 tgtgccatag tgcaggatca ccttaaagtg atgatcatcc acagggtaca ccaccttaaa
     1801 aattttttcg atctggccca tttggtcgcc gctcagacct tcatacggga tgatgacatg
     1861 gatgtcgatc ttcagcccat tttcaccgct caggacaatc ctttggatcg gagttacgga
     1921 caccccgaga ttctgaaaca aactggacac acctccctgt tcaaggactt ggtccaggtt
     1981 gtagccggct gtctgtcgcc agtccccaac gaaatcttcg agtgtgaaga ccatggtgag
     2041 cgctagctgc aaaaagaaca agtagcttgt attctatagt gtcacctaaa tgggcgatct
     2101 gacggttcac taaaccagct ctgcttatat aggcctccct ggcgcgccac tagttattaa
     2161 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
     2221 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     2281 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     2341 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtccgccc
     2401 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     2461 cgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
     2521 cggttttggc agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
     2581 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
     2641 aaatgtcgta ataaccccgc cccgttgacg caaatgggcg gtaggcgtgt acggtgggag
     2701 gtcaaatcga attctaatac tgttgacaat taatcatcgg catagtatat cggcatagta
     2761 taatacgaca aggtgaggaa ctaaacccag gaggcagatc atggagaaaa aaatcactgg
     2821 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc
     2881 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac
     2941 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat
     3001 gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag
     3061 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag
     3121 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta
     3181 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc
     3241 caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt
     3301 cgcccccgtt ttcactatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct
     3361 ggcgattcag gttcatcatg ccgtctgtga tggcttccat gtcggcagaa tgcttaatga
     3421 attacaacag tactgcgatg agtggcaggg cggggcgtaa gcgggactct ggggttcgaa
     3481 atgaccgacc aagcgacgcc caaccggtat cagctcactc aaaggcggta atacggttat
     3541 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     3601 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     3661 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     3721 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     3781 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     3841 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     3901 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     3961 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     4021 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     4081 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     4141 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     4201 gcagaaaaaa aggatttcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     4261 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     4321 agatcctttt atagtccgga atcgtcacac aaaaaaccaa cacacagatg taatgaaaat
     4381 aaagatattt tattgcggcc atcgtgatgg ctagagttca gaagaactcg tcaagaaggc
     4441 gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt
     4501 cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat
     4561 agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca
     4621 ccatgatatt cggcaagcag gcatcgccgt gggtcacgac gagatcctcg ccgtcgggca
     4681 tgcgcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca
     4741 gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt
     4801 tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat
     4861 cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg
     4921 gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg
     4981 cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtcc tgcagttcat
     5041 tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc
     5101 ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc
     5161 tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atggtggcat
     5221 cgattcccgg tcaacatcca gtacacctcc ggcaccaccg gcttccccaa gggcgccacg
     5281 ctctcccacc acaacatcct caacaacggc tactgggtgg gccgcacggt cggctacacg
     5341 gagcaggacc gggtgtgtct gccggtgccc ttctaccact gcttcggcat ggtcatgggc
     5401 aacttgggcg ccacctccca cgccgcctgc atcgtcatcc cggccccgtc cttcgagccg
     5461 gcggcgacgc tggaggcggt gcagcgggag aggtgcacgt ccctgtacgg cgtccccacg
     5521 aagccgaatt ccaccacact ggactagatg catgctcgac agtgtgatgg atatctgcag
     5581 aattcggctt tcacgacacc tgaaatggaa gaaaaaaact ttgaaccact gtctgaggct
     5641 tgagaatgaa ccaagatcca aactcaaaaa gggcaaattc caaggagaat tacatcaagt
     5701 gccaagctgg cctaacttca gtctccaccc actcagtgtg gggaaactcc atcgcataaa
     5761 acccctcccc ccaacctaaa gacgacgtac tccaaaagct cgagaactaa tcgaggtgcc
     5821 tggacggcgc ccggtactcc gtggagtcac atgaagcgac ggctgaggac ggaaaggccc
     5881 ttttcctttg tgtgggtgac tcacccgccc gctctcccga gcgccgcgtc ctccattttg
     5941 agctgcctgc agcagggccg ggaagcggcc atctttccgc tcacgcaact ggtgccgacc
     6001 gggccagcct tgccgcccag ggcggggcga tacacggcgg cgcgaggcca ggcaccagag
     6061 caggccggcc agcttgagac tacccccgtc cgattctcgg tggccgcgct cgcaggcccc
     6121 gcctcgccga acatgtgcgc tgggacgcac gggccccgtc gccgcccgcg gccccaaaaa
     6181 ccgaaatacc agtgtgcaga tcttggcccg catttacaag actatcttgc cagaaaaaaa
     6241 gcgtcgcagc aggtcatcaa aaattttaaa tggctagaga cttatcgaaa gcagcgagac
     6301 aggcgcgaag gtgccaccag attcgcacgc ggcggcccca gcgcccaggc caggcctcaa
     6361 ctcaagcacg aggcgaaggg gctccttaag cgcaaggcct cgaactctcc cacccacttc
     6421 caacccgaag ctcgggatca agaatcacgt actgcagcca ggtggaagta attcaaggca
     6481 cgcaagggcc ataacccgta aagaggccag gcccgcggga accacacacg gcacttacct
     6541 gtgttctggc ggcaaacccg ttgcgaaaaa gaacgttcac ggcgactact gcacttatat
     6601 acggttctcc cccaccctcg ggaaaaaggc ggagccagta cacgacatca ctttcccagt
     6661 ttaccccgcg ccaccttctc taggcaccgg ttcaattgcc gacccctccc cccaacttct
     6721 cggggactgt gggcgatgtg cgctctgccc actgacgggc accggagcc
//
